Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,836,145 C→A M86I (ATG→ATT)  hycI ← protease involved in processing C‑terminal end of HycE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,836,1450CA100.0% 759.3 / NA 225M86I (ATG→ATT) hycIprotease involved in processing C‑terminal end of HycE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (116/109);  total (116/109)

Only 100 of 236 total aligned reads displayed.

AACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTC  >  CP009273/2835998‑2836291
                                                                                                                                                   |                                                                                                                                                  
aaCGGTTTCTACCGCGTCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTcataat                                                                                                                                                  <  2:786418/150‑1 (MQ=255)
 aCGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCataata                                                                                                                                                 <  2:185100/150‑1 (MQ=255)
  cGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCAtaataa                                                                                                                                                >  1:3331691/1‑150 (MQ=255)
   ggTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATaaa                                                                                                                                               >  1:678601/1‑150 (MQ=255)
        cTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACAtct                                                                                                                                          >  1:2713187/1‑150 (MQ=255)
          aCCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGATAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCg                                                                                                                                        <  1:1121172/150‑1 (MQ=255)
          aCCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCg                                                                                                                                        <  1:3487423/150‑1 (MQ=255)
            cGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGc                                                                                                                                      <  1:3371391/150‑1 (MQ=255)
             gCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCg                                                                                                                                     <  2:3610508/150‑1 (MQ=255)
               aTCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGat                                                                                                                                   <  1:1965171/150‑1 (MQ=255)
                tCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGata                                                                                                                                  >  2:149340/1‑150 (MQ=255)
                tCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGata                                                                                                                                  <  1:2300535/150‑1 (MQ=255)
                 cTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGatat                                                                                                                                 >  2:85150/1‑150 (MQ=255)
                 cTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGatat                                                                                                                                 >  1:1365805/1‑150 (MQ=255)
                   ttAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATatca                                                                                                                               >  2:3528353/1‑150 (MQ=255)
                         ggCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCggg                                                                                                                         <  2:2666072/150‑1 (MQ=255)
                          gCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGt                                                                                                                        <  2:422591/150‑1 (MQ=255)
                           cTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTc                                                                                                                       <  1:1906382/150‑1 (MQ=255)
                                 cATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCgatgat                                                                                                                 >  2:2357000/1‑150 (MQ=255)
                                  aTCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAATTCACTTCGCCAATATCTACTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATAACATCCGGGTCGatgatg                                                                                                                >  1:424651/1‑150 (MQ=255)
                                   tCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGc                                                                                                               <  2:3479336/150‑1 (MQ=255)
                                    cGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCg                                                                                                              <  1:1800955/150‑1 (MQ=255)
                                    cGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTATTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCg                                                                                                              <  2:332906/150‑1 (MQ=255)
                                     gggTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCgg                                                                                                             >  2:2988677/1‑150 (MQ=255)
                                       gtagtaAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGAt                                                                                                           <  2:3484388/150‑1 (MQ=255)
                                        tagtaAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGAtc                                                                                                          >  1:1644239/1‑150 (MQ=255)
                                           taAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGAtctca                                                                                                       <  1:868657/150‑2 (MQ=255)
                                           taAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCg                                                                                                       <  2:1272694/150‑1 (MQ=255)
                                             aaaGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGcc                                                                                                     <  2:876463/150‑1 (MQ=255)
                                               aGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCgg                                                                                                   >  1:1675864/1‑150 (MQ=255)
                                                 ccGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCgggg                                                                                                 >  2:578499/1‑150 (MQ=255)
                                                 ccGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCgggg                                                                                                 >  2:1451905/1‑150 (MQ=255)
                                                    acgaTATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGttt                                                                                              <  2:1797231/150‑1 (MQ=255)
                                                    acgaTATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGttt                                                                                              <  1:105730/150‑1 (MQ=255)
                                                     cgaTATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTa                                                                                             <  2:889591/150‑1 (MQ=255)
                                                      gaTATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAg                                                                                            >  1:1025864/1‑150 (MQ=255)
                                                       atatCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGc                                                                                           <  2:678601/150‑1 (MQ=255)
                                                         atCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGccc                                                                                         <  2:573542/150‑1 (MQ=255)
                                                         atCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGccc                                                                                         >  1:2773710/1‑150 (MQ=255)
                                                            cGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCat                                                                                      <  2:372336/150‑1 (MQ=255)
                                                            cGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTAAGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCat                                                                                      <  1:105124/150‑1 (MQ=255)
                                                              gCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCatat                                                                                    <  1:3577472/150‑1 (MQ=255)
                                                              gCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCatat                                                                                    <  1:3577666/150‑1 (MQ=255)
                                                               cTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATc                                                                                   <  1:2151898/150‑1 (MQ=255)
                                                               cTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATc                                                                                   <  1:1565283/150‑1 (MQ=255)
                                                               cTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATc                                                                                   <  1:2246256/150‑1 (MQ=255)
                                                                 gAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCg                                                                                 <  2:2585035/150‑1 (MQ=255)
                                                                  aaTGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCCACGGGTCGATAAGGGAATTCAACGGCCTGCTATGGGTAGGCATAATAAACATCTCGGCGATTGCTGCCGGGGCGCTGGTGCGGGTCTCGGCGGGGGTTGGCCCCataggggg                                                                                >  2:3672633/1‑145 (MQ=255)
                                                                  aaTGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGt                                                                                <  1:74525/150‑1 (MQ=255)
                                                                  aaTGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGt                                                                                >  2:2449531/1‑150 (MQ=255)
                                                                           gAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGAc                                                                       >  1:2511474/1‑150 (MQ=255)
                                                                            aaaaTCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACa                                                                      <  2:2134026/150‑1 (MQ=255)
                                                                              aaTCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAAt                                                                    >  1:2802909/1‑150 (MQ=255)
                                                                              aaTCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAAt                                                                    <  2:542979/150‑1 (MQ=255)
                                                                                tCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATca                                                                  >  2:733620/1‑150 (MQ=255)
                                                                                tCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATca                                                                  >  2:2812173/1‑150 (MQ=255)
                                                                                tCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATca                                                                  >  1:2902485/1‑150 (MQ=255)
                                                                                tCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATca                                                                  >  2:465958/1‑150 (MQ=255)
                                                                                 cACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATcag                                                                 <  2:2215679/150‑1 (MQ=255)
                                                                                  aCTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATcagc                                                                <  2:1319170/150‑1 (MQ=255)
                                                                                    ttCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATcagcag                                                              <  1:3519155/150‑1 (MQ=255)
                                                                                     tCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGt                                                             >  2:11608/1‑150 (MQ=255)
                                                                                        ccAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCgt                                                          <  1:3794579/150‑1 (MQ=255)
                                                                                        ccAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCgt                                                          >  2:1221532/1‑150 (MQ=255)
                                                                                         cAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCgtg                                                         >  1:621486/1‑150 (MQ=255)
                                                                                         cAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCgtg                                                         >  1:2203507/1‑150 (MQ=255)
                                                                                          aaTATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCgtgt                                                        >  2:340702/1‑150 (MQ=255)
                                                                                           atatCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTc                                                       >  1:1080797/1‑150 (MQ=255)
                                                                                           atatCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTc                                                       >  1:150041/1‑150 (MQ=255)
                                                                                            tatCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCg                                                      <  1:3258859/150‑1 (MQ=255)
                                                                                            tatCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCg                                                      >  1:2552117/1‑150 (MQ=255)
                                                                                             atCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCgg                                                     >  1:2979444/1‑150 (MQ=255)
                                                                                              tcttctTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCggg                                                    >  1:3223402/1‑150 (MQ=255)
                                                                                                    ttCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACACTCAGCAGTCGTGTCGGGCGCAGt                                              >  1:2267497/1‑150 (MQ=255)
                                                                                                    ttCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGt                                              >  1:1139108/1‑150 (MQ=255)
                                                                                                    ttCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGt                                              >  2:3352676/1‑150 (MQ=255)
                                                                                                    ttCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGt                                              >  1:3531798/1‑150 (MQ=255)
                                                                                                      cAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTc                                            >  2:1423483/1‑150 (MQ=255)
                                                                                                      cAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTc                                            <  2:1036516/150‑1 (MQ=255)
                                                                                                         cTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACg                                         <  2:2771738/150‑1 (MQ=255)
                                                                                                            gTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGAt                                      <  2:3772826/150‑1 (MQ=255)
                                                                                                              cGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAg                                    <  2:519941/150‑1 (MQ=255)
                                                                                                               gATAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGc                                   >  1:3272390/1‑150 (MQ=255)
                                                                                                                 tAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGcga                                 <  2:621486/150‑1 (MQ=255)
                                                                                                                 tAAGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGcga                                 <  2:2005960/150‑1 (MQ=255)
                                                                                                                   aGGTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGc                                   >  2:2858773/1‑146 (MQ=255)
                                                                                                                    ggTAATTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGcgacga                              <  2:2324955/150‑1 (MQ=255)
                                                                                                                       aaTTCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGt                           >  1:3079712/1‑150 (MQ=255)
                                                                                                                         ttCAACGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCg                         >  1:956186/1‑150 (MQ=255)
                                                                                                                            aaCGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGttt                      >  2:2604939/1‑150 (MQ=255)
                                                                                                                              cGGCATGTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTc                    <  1:1222031/150‑1 (MQ=255)
                                                                                                                                    gTTATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTgc              <  1:3161324/150‑1 (MQ=255)
                                                                                                                                      tATGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTgcgc            >  2:3636655/1‑150 (MQ=255)
                                                                                                                                       aTGGGTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCt           >  2:3077164/1‑150 (MQ=255)
                                                                                                                                          ggTAGTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTAcc        <  1:2348367/150‑1 (MQ=255)
                                                                                                                                              gTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTAccgccg    >  2:1133440/1‑150 (MQ=255)
                                                                                                                                              gTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTAccgccg    <  1:1466815/150‑1 (MQ=255)
                                                                                                                                              gTCATAATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTAccgccg    >  2:810646/1‑150 (MQ=255)
                                                                                                                                               tcataaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGt   >  1:3656907/1‑150 (MQ=255)
                                                                                                                                               tcataaTAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGt   >  1:1605764/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCACTTCGCCAATATCTTCTTTCAACTGGTCGATAAGGTAATTCAACGGCATGTTATGGGTAGTCATCATAAACATCTCGGCGATATCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTC  >  CP009273/2835998‑2836291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑