Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,869,780 G→A intergenic (‑92/‑160) cysD ← / → iap sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,869,7800GA99.5% 683.6 / NA 207intergenic (‑92/‑160)cysD/iapsulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (100/106);  minor base T (0/1);  total (100/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

ATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGG  >  CP009273/2869633‑2869923
                                                                                                                                                   |                                                                                                                                               
atGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAag                                                                                                                                               <  2:1244009/150‑1 (MQ=255)
 tgtgGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAagt                                                                                                                                              <  2:2918098/150‑1 (MQ=255)
   tgGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAagtag                                                                                                                                            <  2:2214170/150‑1 (MQ=255)
    ggATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAagtagt                                                                                                                                           >  1:2575196/1‑150 (MQ=255)
     gATGCTTTCCGTCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGtt                                                                                                                                          <  2:2961939/150‑1 (MQ=255)
        gCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCt                                                                                                                                       >  2:2830348/1‑150 (MQ=255)
         cTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCtt                                                                                                                                      <  1:1254240/150‑1 (MQ=255)
         cTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCtt                                                                                                                                      <  2:277892/150‑1 (MQ=255)
         cTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCtt                                                                                                                                      <  2:278288/150‑1 (MQ=255)
           ttCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTaa                                                                                                                                    >  2:808577/1‑150 (MQ=255)
           ttCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTaa                                                                                                                                    >  2:2035536/1‑150 (MQ=255)
            tCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAAc                                                                                                                                   <  1:1009095/150‑1 (MQ=255)
            tCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAAc                                                                                                                                   <  2:1645643/150‑1 (MQ=255)
            tCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAAc                                                                                                                                   <  2:3156169/150‑1 (MQ=255)
            tCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAAc                                                                                                                                   <  2:3539938/150‑1 (MQ=255)
             ccGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACg                                                                                                                                  <  1:1181142/150‑1 (MQ=255)
               gCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGa                                                                                                                                >  2:2426841/1‑150 (MQ=255)
                 cTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAAt                                                                                                                              >  2:1917665/1‑150 (MQ=255)
                  tCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATa                                                                                                                             <  2:3785804/150‑1 (MQ=255)
                  tCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATa                                                                                                                             <  2:3348345/150‑1 (MQ=255)
                      gTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGa                                                                                                                         >  1:2715326/1‑150 (MQ=255)
                       ttGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGAt                                                                                                                        <  2:1735992/150‑1 (MQ=255)
                        tGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGAtt                                                                                                                       >  1:709493/1‑150 (MQ=255)
                        tGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGAtt                                                                                                                       >  2:3304751/1‑150 (MQ=255)
                          ccGCAGGTGAGTAAGTCGTATTTGATCCATAACCGGTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTAGAGGGAGCGTTAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTg                                                                                                                     <  1:2979910/150‑1 (MQ=255)
                             cAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCa                                                                                                                  <  1:3509372/150‑1 (MQ=255)
                                gTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAg                                                                                                               <  1:727750/150‑1 (MQ=255)
                                 tGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGc                                                                                                              <  1:943939/150‑1 (MQ=255)
                                  gAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCt                                                                                                             <  1:2300173/150‑1 (MQ=255)
                                      aaGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAata                                                                                                         <  2:1390691/150‑1 (MQ=255)
                                          cGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCaa                                                                                                     >  2:431028/1‑150 (MQ=255)
                                          cGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCaa                                                                                                     >  1:3780464/1‑150 (MQ=255)
                                             aTTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCaaaaa                                                                                                  >  2:2730022/1‑150 (MQ=255)
                                               ttGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGt                                                                                                <  2:650996/150‑1 (MQ=255)
                                                tGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTg                                                                                               >  2:1312259/1‑150 (MQ=255)
                                                 gATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGc                                                                                              >  1:1783766/1‑150 (MQ=255)
                                                  aTCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGGGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCt                                                                                             <  2:2529021/150‑1 (MQ=255)
                                                   tCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCtt                                                                                            <  1:726076/150‑1 (MQ=255)
                                                    ccATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTa                                                                                           >  1:161057/1‑150 (MQ=255)
                                                        aaCCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGc                                                                                       >  1:1936930/1‑150 (MQ=255)
                                                           cGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCAcc                                                                                    <  2:1840072/150‑1 (MQ=255)
                                                            gTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCg                                                                                   >  2:963918/1‑150 (MQ=255)
                                                            gTTCCTGTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATTAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCg                                                                                   <  2:3463102/150‑1 (MQ=255)
                                                              tCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGa                                                                                 >  1:3407822/1‑150 (MQ=255)
                                                                  ttGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTc                                                                             >  1:2009789/1‑150 (MQ=255)
                                                                    gCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCgg                                                                           <  2:2310705/150‑1 (MQ=255)
                                                                    gCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCgg                                                                           >  2:733507/1‑150 (MQ=255)
                                                                      aaTACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGc                                                                         >  2:1679946/1‑150 (MQ=255)
                                                                         aCCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGtt                                                                      >  2:509587/1‑150 (MQ=255)
                                                                         aCCGCTATTTTCTTGCCATCAGATGTGTCGACTATAGGGAGCGTAAGAGAACGACTGAAATTACCACTTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCACAAAGTGCTTAAGGCACCGGATTTCGGGCGtt                                                                      <  2:102199/150‑1 (MQ=255)
                                                                           cGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTa                                                                    >  2:1929810/1‑150 (MQ=255)
                                                                             cTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAgg                                                                  >  1:1432260/1‑150 (MQ=255)
                                                                              tATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGa                                                                 >  2:2639222/1‑150 (MQ=255)
                                                                              tATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGa                                                                 >  1:797071/1‑150 (MQ=255)
                                                                              tATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGa                                                                 >  2:494779/1‑150 (MQ=255)
                                                                                    cTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGAttt                                                           <  1:3805777/150‑1 (MQ=255)
                                                                                     ttGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTg                                                          >  2:2703575/1‑150 (MQ=255)
                                                                                        ccATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGaaa                                                       <  1:431028/150‑1 (MQ=255)
                                                                                         cATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAt                                                      >  1:3632415/1‑150 (MQ=255)
                                                                                         cATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAt                                                      >  2:3366361/1‑150 (MQ=255)
                                                                                          aTCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAtt                                                     <  1:618875/150‑1 (MQ=255)
                                                                                             ggATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGtt                                                  >  2:3754276/2‑150 (MQ=255)
                                                                                              gATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGttt                                                 <  2:1034084/150‑1 (MQ=255)
                                                                                              gATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGttt                                                 <  2:3484564/150‑1 (MQ=255)
                                                                                                tGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTa                                               <  2:709493/150‑1 (MQ=255)
                                                                                                tGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTa                                               >  1:2240522/1‑150 (MQ=255)
                                                                                                tGTTTAGACTATAGGGAGCGTAATAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTa                                               <  2:3505906/150‑1 (MQ=255)
                                                                                                 gTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAg                                              <  2:1490796/150‑1 (MQ=255)
                                                                                                   ttCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAgcg                                            >  1:1530874/1‑150 (MQ=255)
                                                                                                   ttCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAgcg                                            >  2:880185/1‑150 (MQ=255)
                                                                                                     cGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCa                                          >  1:1799395/1‑150 (MQ=255)
                                                                                                     cGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCa                                          >  1:3206287/1‑150 (MQ=255)
                                                                                                     cGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCa                                          >  2:1879886/1‑150 (MQ=255)
                                                                                                       aCTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACTGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGc                                        >  1:3686509/1‑150 (MQ=255)
                                                                                                            aGGGAGAGTAAGAGAAAGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGATAATTTCAAAAAGTGCTTAAGGCACCGGATTTCGGTCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAg                                   <  2:772690/150‑1 (MQ=255)
                                                                                                              ggAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGtt                                 >  2:2198449/1‑150 (MQ=255)
                                                                                                               gAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGttt                                <  2:74696/150‑1 (MQ=255)
                                                                                                                  cGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCAt                             <  2:1221929/150‑1 (MQ=255)
                                                                                                                   gTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATa                            <  1:1682118/150‑1 (MQ=255)
                                                                                                                    tAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATAc                           <  2:1280537/150‑1 (MQ=255)
                                                                                                                     aaGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACt                          <  1:358364/150‑1 (MQ=255)
                                                                                                                       gagaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTAt                        >  2:793803/1‑150 (MQ=255)
                                                                                                                       gagaAAGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAAAGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCTTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTAt                        <  1:1968304/150‑1 (MQ=255)
                                                                                                                        agaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGCTTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATg                       >  1:1125576/1‑150 (MQ=255)
                                                                                                                        agaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATg                       >  2:3684357/1‑150 (MQ=255)
                                                                                                                        agaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATg                       >  1:3051145/1‑150 (MQ=255)
                                                                                                                        agaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATg                       >  2:2353295/1‑150 (MQ=255)
                                                                                                                         gaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATgg                      <  1:2041911/150‑1 (MQ=255)
                                                                                                                          aaCGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGc                     <  1:337338/150‑1 (MQ=255)
                                                                                                                           aCGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCg                    <  1:2426841/150‑1 (MQ=255)
                                                                                                                            cGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCgg                   >  2:360219/1‑150 (MQ=255)
                                                                                                                            cGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCgg                   <  2:1172189/150‑1 (MQ=255)
                                                                                                                                tGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaaa               >  1:2239208/1‑150 (MQ=255)
                                                                                                                                tGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaaa               >  1:65398/1‑150 (MQ=255)
                                                                                                                                       aCCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGAGTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATCCTATGGCGGTGAATGAAttt        >  2:3214040/1‑150 (MQ=255)
                                                                                                                                        ccAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTg       <  1:888909/150‑1 (MQ=255)
                                                                                                                                           aTTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCAt    <  2:2239208/150‑1 (MQ=255)
                                                                                                                                           aTTAGAATAAGTAGGTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCAt    <  1:138196/150‑1 (MQ=255)
                                                                                                                                             tAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATgg  <  2:766500/150‑1 (MQ=255)
                                                                                                                                             tAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATgg  <  2:767260/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGG  >  CP009273/2869633‑2869923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑