Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,879,449 G→T S377* (TCA→TAA)  ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,879,4490GT100.0% 804.8 / NA 241S377* (TCA→TAA) ygcBR‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (100/141);  total (100/141)

Only 100 of 241 total aligned reads displayed.

TGATCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTGAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTCCAA  >  CP009273/2879302‑2879595
                                                                                                                                                   |                                                                                                                                                  
tGATCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTaa                                                                                                                                                  <  1:3322133/150‑1 (MQ=255)
  aTCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAAtt                                                                                                                                                >  2:2413090/1‑150 (MQ=255)
  aTCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAAtt                                                                                                                                                <  1:3373080/150‑1 (MQ=255)
           gCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGc                                                                                                                                       <  1:2114353/150‑1 (MQ=255)
            cAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCa                                                                                                                                      <  1:1187960/150‑1 (MQ=255)
            cAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCa                                                                                                                                      <  2:3625131/150‑1 (MQ=255)
             aaaCGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCaa                                                                                                                                     <  1:2198113/150‑1 (MQ=255)
                cGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGaa                                                                                                                                  <  2:122685/150‑1 (MQ=255)
                cGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGaa                                                                                                                                  >  1:3329743/1‑150 (MQ=255)
                cGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGaa                                                                                                                                  >  2:1806496/1‑150 (MQ=255)
                 gCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAAt                                                                                                                                 >  1:1238821/1‑150 (MQ=255)
                   cGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATaa                                                                                                                               >  2:3076842/1‑150 (MQ=255)
                   cGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATGGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATaa                                                                                                                               >  2:3510782/1‑150 (MQ=255)
                    gATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAg                                                                                                                              <  2:2943336/150‑1 (MQ=255)
                     aTTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGa                                                                                                                             <  1:722597/150‑1 (MQ=255)
                      tttGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTCCGCGCGCGTGATTTTATTGATTGACAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGAt                                                                                                                            >  2:163964/1‑150 (MQ=255)
                       ttGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGAtt                                                                                                                           <  1:2795710/150‑1 (MQ=255)
                        tGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGAttt                                                                                                                          >  2:2660664/1‑150 (MQ=255)
                        tGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGAttt                                                                                                                          <  2:2111144/150‑1 (MQ=255)
                        tGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGAttt                                                                                                                          <  2:2283294/150‑1 (MQ=255)
                          cccAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGTTGGTTAAACCGTTAATTTCCATTAGCAAGAATAAGATTTgg                                                                                                                        >  1:3065153/1‑150 (MQ=255)
                           ccAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTggg                                                                                                                       <  1:2361790/150‑1 (MQ=255)
                            cAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTgggg                                                                                                                      <  2:1966154/150‑1 (MQ=255)
                             aaGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGa                                                                                                                     <  1:1930597/150‑1 (MQ=255)
                              aGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGAt                                                                                                                    >  2:2517958/1‑150 (MQ=255)
                               gAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATg                                                                                                                   <  1:611911/150‑1 (MQ=255)
                                aaaCACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGa                                                                                                                  <  1:275002/150‑1 (MQ=255)
                                aaaCACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGa                                                                                                                  >  2:3651030/1‑150 (MQ=255)
                                aaaCACTTTCTTAGTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGa                                                                                                                  <  1:2982680/150‑1 (MQ=255)
                                  acacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaa                                                                                                                >  1:829205/1‑150 (MQ=255)
                                   cacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaaa                                                                                                               >  1:815231/1‑150 (MQ=255)
                                   cacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaaa                                                                                                               >  2:714774/1‑150 (MQ=255)
                                   cacTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAAGCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGaaaa                                                                                                               >  2:3110931/1‑150 (MQ=255)
                                     cTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTACCATGAGCAAGAATAAGATTTGGGGATGAAAATa                                                                                                             >  1:1156725/1‑150 (MQ=255)
                                        tCTTATTGCTTTGTGACACCCACTGACACCCCTGAACCCCCGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGGGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGt                                                                                                          <  2:3439784/150‑1 (MQ=255)
                                         cTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTg                                                                                                         <  1:2627740/150‑1 (MQ=255)
                                         cTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTg                                                                                                         <  1:576260/150‑1 (MQ=255)
                                          ttATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTgg                                                                                                        >  1:488924/1‑150 (MQ=255)
                                           tATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgc                                                                                                       <  1:2604099/150‑1 (MQ=255)
                                             ttGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgctc                                                                                                     <  2:1529395/150‑1 (MQ=255)
                                             ttGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgctc                                                                                                     <  2:1303068/150‑1 (MQ=255)
                                                cTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgctcgcg                                                                                                  >  1:2252621/1‑150 (MQ=255)
                                                   tgtgACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGgctcgcgctc                                                                                               >  1:172485/1‑150 (MQ=255)
                                                       aCAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCtt                                                                                           <  2:3712487/150‑1 (MQ=255)
                                                       aCAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCtt                                                                                           <  2:1595485/150‑1 (MQ=255)
                                                        cAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGACAATAAGTGGCTCGCGCTCGCTTc                                                                                          >  2:583723/1‑150 (MQ=255)
                                                           ccACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCAt                                                                                       <  1:1806496/150‑1 (MQ=255)
                                                            cACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCAtt                                                                                      >  2:1078349/1‑150 (MQ=255)
                                                              cTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATtct                                                                                    >  1:570349/1‑150 (MQ=255)
                                                               tGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATtctc                                                                                   <  2:838794/150‑1 (MQ=255)
                                                               tGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATtctc                                                                                   >  2:2220080/1‑150 (MQ=255)
                                                                 acaacaCTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGt                                                                                 <  2:1463400/150‑1 (MQ=255)
                                                                  caacaCTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTa                                                                                >  2:219054/1‑150 (MQ=255)
                                                                   aacaCTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTaa                                                                               <  2:3265994/150‑1 (MQ=255)
                                                                         gAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagcatag                                                                         >  2:3662687/1‑150 (MQ=255)
                                                                           aCCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAagcatagca                                                                       >  1:1031934/1‑150 (MQ=255)
                                                                             ccACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCAtt                                                                     <  2:414724/150‑1 (MQ=255)
                                                                                 gCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTcgcg                                                                 >  1:3704156/1‑150 (MQ=255)
                                                                                 gCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTcgcg                                                                 >  2:635725/1‑150 (MQ=255)
                                                                                   ttcttcttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGt                                                               <  2:2838031/150‑1 (MQ=255)
                                                                                       tcttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCt                                                           <  2:3273434/150‑1 (MQ=255)
                                                                                        cttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCtt                                                          <  1:3502984/150‑1 (MQ=255)
                                                                                         ttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTtg                                                         <  2:488924/150‑1 (MQ=255)
                                                                                         ttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTtg                                                         <  1:2417268/150‑1 (MQ=255)
                                                                                         ttGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTtg                                                         >  1:101006/1‑150 (MQ=255)
                                                                                            ccccTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTgtgt                                                      >  1:2477404/1‑150 (MQ=255)
                                                                                             cccTGTTCAGTACTCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGtt                                                     <  1:2517958/150‑1 (MQ=255)
                                                                                             cccTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGtt                                                     <  1:354808/150‑1 (MQ=255)
                                                                                             cccTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGtt                                                     <  1:2297358/150‑1 (MQ=255)
                                                                                              ccTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTg                                                    <  2:2313939/150‑1 (MQ=255)
                                                                                                tGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTggg                                                  >  1:2555009/1‑150 (MQ=255)
                                                                                                 gTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGa                                                 <  2:895993/150‑1 (MQ=255)
                                                                                                 gTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGa                                                 >  2:1128475/1‑150 (MQ=255)
                                                                                                     aGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGc                                             >  1:1336187/1‑150 (MQ=255)
                                                                                                      gTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCa                                            <  2:494627/150‑1 (MQ=255)
                                                                                                        aaTCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCaaa                                          >  2:407307/1‑150 (MQ=255)
                                                                                                         aTCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCaaaa                                         <  2:1432264/150‑1 (MQ=255)
                                                                                                                  gATTTTATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTa                                <  2:2808775/150‑1 (MQ=255)
                                                                                                                    ttttATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATc                              <  2:2443866/150‑1 (MQ=255)
                                                                                                                     tttATTGTTTGAAAGAGGGGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAAGAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATcc                             <  2:2014071/150‑1 (MQ=255)
                                                                                                                     tttATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAATATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATcc                             <  1:2818933/150‑1 (MQ=255)
                                                                                                                     tttATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATcc                             >  1:113600/1‑150 (MQ=255)
                                                                                                                     tttATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATcc                             <  2:3153315/150‑1 (MQ=255)
                                                                                                                      ttATTGATTGAAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCg                            <  2:3366024/150‑1 (MQ=255)
                                                                                                                           gattgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAAtt                       >  2:1174147/1‑150 (MQ=255)
                                                                                                                           gattgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAAtt                       <  1:768977/150‑1 (MQ=255)
                                                                                                                           gattgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAAtt                       >  1:1893260/1‑150 (MQ=255)
                                                                                                                            attgaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAAttt                      <  2:3346117/150‑1 (MQ=255)
                                                                                                                               gaAAGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATttgtt                   <  1:3186295/150‑1 (MQ=255)
                                                                                                                                aaaGAGGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATttgttg                  <  1:949882/150‑1 (MQ=255)
                                                                                                                                   gagGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCCATTTGTTGATc               >  1:2399971/1‑150 (MQ=255)
                                                                                                                                   gagGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATc               <  1:1458933/150‑1 (MQ=255)
                                                                                                                                    agGTGGTTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCa              <  2:3463376/150‑1 (MQ=255)
                                                                                                                                     ggtggtTAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCaa             <  2:1238821/150‑1 (MQ=255)
                                                                                                                                         gtTAAACCGTTAAGTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAg         <  2:2865478/150‑1 (MQ=255)
                                                                                                                                          ttAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGt        <  2:392550/150‑1 (MQ=255)
                                                                                                                                          ttAAACCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGt        <  2:1027883/150‑1 (MQ=255)
                                                                                                                                              aCCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTcc    <  2:152651/150‑1 (MQ=255)
                                                                                                                                              aCCGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGGTGGGAGGGCAAAAATAACACTCTCCGCACTTTGTTGATCAATAAGTTTcc    >  2:2759060/1‑150 (MQ=255)
                                                                                                                                                cGTTAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTCCaa  >  2:3016079/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGATCAATCGTGCAAACGCCGATTTGCCCAAGAAACACTTTCTTATTGCTTTGTGACAACCACTGACAACACTGAACCCACGCTTCTTCTTGCCCCTGTTCAGTAATCGCGCGTGATTTTATTGATTGAAAGAGGTGGTTAAACCGTGAATTGCCATGAGCAAGAATAAGATTTGGGGATGAAAATAAGTGGCTCGCGCTCGCTTCCATTCTCGTAAGCATAGCATTCGCGGTAGCTTGTGTTGGGAGGGCAAAAATAACACTATCCGCAATTTGTTGATCAATAAGTTTCCAA  >  CP009273/2879302‑2879595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑