Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,939,832 C→A E236* (GAA→TAA)  mltA ← membrane‑bound lytic murein transglycosylase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,939,8320CA100.0% 715.1 / NA 215E236* (GAA→TAA) mltAmembrane‑bound lytic murein transglycosylase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (108/107);  total (108/107)

Only 100 of 215 total aligned reads displayed.

AATGGAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCG  >  CP009273/2939686‑2939977
                                                                                                                                                  |                                                                                                                                                 
aatGGAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGcc                                                                                                                                                <  1:2972110/150‑1 (MQ=255)
      aCGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTg                                                                                                                                          <  1:819859/150‑1 (MQ=255)
          tCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGa                                                                                                                                      >  2:3716847/1‑150 (MQ=255)
              agGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCg                                                                                                                                  >  1:50639/1‑150 (MQ=255)
               ggCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGc                                                                                                                                 >  2:1017454/1‑150 (MQ=255)
                gCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGcc                                                                                                                                <  2:1448604/150‑1 (MQ=255)
                 cAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCt                                                                                                                               <  1:2893995/150‑1 (MQ=255)
                   aCTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGc                                                                                                                             <  1:2014468/150‑1 (MQ=255)
                    cTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCa                                                                                                                            <  2:2288756/150‑1 (MQ=255)
                     tGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCAt                                                                                                                           <  2:1361513/150‑1 (MQ=255)
                        cgcgcgACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGa                                                                                                                        <  2:2529853/150‑1 (MQ=255)
                         gcgcgACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGAc                                                                                                                       <  2:3199031/150‑1 (MQ=255)
                          cgcgACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGTCGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACa                                                                                                                      <  1:249708/150‑1 (MQ=255)
                          cgcgACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACa                                                                                                                      <  1:1623695/150‑1 (MQ=255)
                            cgACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACata                                                                                                                    >  1:2747742/1‑150 (MQ=255)
                            cgACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACata                                                                                                                    >  2:609061/1‑150 (MQ=255)
                             gACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACatat                                                                                                                   <  2:544574/150‑1 (MQ=255)
                              accaaccaGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATAtc                                                                                                                  >  2:2839031/1‑150 (MQ=255)
                               ccaaccaGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATAtct                                                                                                                 <  2:906903/150‑1 (MQ=255)
                                 aaccaGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATAtcttc                                                                                                               >  1:413249/1‑150 (MQ=255)
                                    caGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCttt                                                                                                            <  2:1897920/150‑1 (MQ=255)
                                     aGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAACGATTGCGGTTTCAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCtttt                                                                                                           <  2:1785490/150‑1 (MQ=255)
                                       cGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCtttttt                                                                                                         >  2:2774160/1‑150 (MQ=255)
                                        ggCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTc                                                                                                        >  2:1173756/1‑150 (MQ=255)
                                        ggCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTc                                                                                                        >  1:1455750/1‑150 (MQ=255)
                                           aCCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACt                                                                                                     <  2:2672673/150‑1 (MQ=255)
                                           aCCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACt                                                                                                     <  1:3392308/150‑1 (MQ=255)
                                            ccGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACtt                                                                                                    <  2:41465/150‑1 (MQ=255)
                                              gCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCg                                                                                                  >  1:3022478/1‑150 (MQ=255)
                                                aCTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGcc                                                                                                >  2:568005/1‑150 (MQ=255)
                                                      cccTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTc                                                                                          <  2:3441264/150‑1 (MQ=255)
                                                      cccTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTc                                                                                          >  2:1846817/1‑150 (MQ=255)
                                                        cTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGa                                                                                        <  2:3123614/150‑1 (MQ=255)
                                                        cTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGa                                                                                        >  1:3564737/1‑150 (MQ=255)
                                                           tCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCa                                                                                     >  2:355216/1‑150 (MQ=255)
                                                               cGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCAc                                                                                 <  2:3390320/150‑1 (MQ=255)
                                                               cGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCAc                                                                                 >  1:871853/1‑150 (MQ=255)
                                                                ggAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCAcc                                                                                <  1:1739293/150‑1 (MQ=255)
                                                                ggAGCAAAAGAGTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCAcc                                                                                <  1:1640691/150‑1 (MQ=255)
                                                                 gAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCt                                                                               <  1:1388267/150‑1 (MQ=255)
                                                                 gAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCt                                                                               <  2:1575729/150‑1 (MQ=255)
                                                                  aGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACAGATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCtt                                                                              >  2:2213499/1‑150 (MQ=255)
                                                                    cAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTAc                                                                            >  1:1014971/1‑150 (MQ=255)
                                                                    cAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTAc                                                                            <  2:2911412/150‑1 (MQ=255)
                                                                    cAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTAc                                                                            >  2:1977148/1‑150 (MQ=255)
                                                                    cAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTAc                                                                            >  2:2476047/1‑150 (MQ=255)
                                                                    cAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTAc                                                                            >  1:3200682/1‑150 (MQ=255)
                                                                     aaaaGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTAcc                                                                           <  1:122336/150‑1 (MQ=255)
                                                                      aaaGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCa                                                                          >  2:2318062/1‑150 (MQ=255)
                                                                        aGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAAt                                                                        >  1:1142144/1‑150 (MQ=255)
                                                                          aTTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAAtgc                                                                      >  1:1102063/1‑150 (MQ=255)
                                                                           ttGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAAtgct                                                                     <  2:2407471/150‑1 (MQ=255)
                                                                              cGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCg                                                                  >  1:2795363/1‑150 (MQ=255)
                                                                               ggTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGa                                                                 >  1:3420335/1‑150 (MQ=255)
                                                                                gTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGAt                                                                <  2:3615225/150‑1 (MQ=255)
                                                                                  ttAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAg                                                              <  2:2126922/150‑1 (MQ=255)
                                                                                     aagaagACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCa                                                           >  2:18438/1‑150 (MQ=255)
                                                                                      agaagaCGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCAt                                                          <  2:18356/150‑1 (MQ=255)
                                                                                       gaagaCGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATg                                                         >  2:1657060/1‑150 (MQ=255)
                                                                                         agaCGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGAc                                                       >  2:680580/1‑150 (MQ=255)
                                                                                           aCGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCg                                                     <  1:2149728/150‑1 (MQ=255)
                                                                                           aCGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCg                                                     <  2:805604/150‑1 (MQ=255)
                                                                                            cGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGt                                                    >  1:770822/1‑150 (MQ=255)
                                                                                             gAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGtt                                                   >  1:2087822/1‑150 (MQ=255)
                                                                                             gAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGtt                                                   >  1:2809614/1‑150 (MQ=255)
                                                                                             gAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGtt                                                   >  1:2328796/1‑150 (MQ=255)
                                                                                              aaaGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGttt                                                  >  2:106105/1‑150 (MQ=255)
                                                                                               aaGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGtttt                                                 >  1:1533942/1‑150 (MQ=255)
                                                                                                aGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGttttt                                                <  1:1263454/150‑1 (MQ=255)
                                                                                                   cGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTccc                                             <  1:2161143/150‑1 (MQ=255)
                                                                                                    gggTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCt                                            <  1:1293732/150‑1 (MQ=255)
                                                                                                      gTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGc                                          >  2:3067017/1‑150 (MQ=255)
                                                                                                      gTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGc                                          >  1:3100659/1‑150 (MQ=255)
                                                                                                      gTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGc                                          >  1:1202348/1‑150 (MQ=255)
                                                                                                      gTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGc                                          >  2:3402120/1‑150 (MQ=255)
                                                                                                      gTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGc                                          >  2:3014243/1‑150 (MQ=255)
                                                                                                       ttCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCa                                         <  1:2318062/150‑1 (MQ=255)
                                                                                                         cTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATa                                       >  1:944428/1‑150 (MQ=255)
                                                                                                          tGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAg                                      <  1:3716847/150‑1 (MQ=255)
                                                                                                           gTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGc                                     >  2:25813/1‑150 (MQ=255)
                                                                                                             tCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTg                                   >  1:3162247/1‑150 (MQ=255)
                                                                                                              ccAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGa                                  <  2:872805/150‑1 (MQ=255)
                                                                                                                 gcagcTCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGaaaa                               >  1:659177/1‑150 (MQ=255)
                                                                                                                      tCGCGAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTa                          >  2:450245/1‑150 (MQ=255)
                                                                                                                        gcgAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAg                        <  1:3624992/150‑1 (MQ=255)
                                                                                                                         cgAACCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGc                       >  2:1920925/1‑150 (MQ=255)
                                                                                                                           aaCCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCgg                     >  1:2555949/1‑150 (MQ=255)
                                                                                                                            aCCTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGa                    <  1:651494/150‑1 (MQ=255)
                                                                                                                             ccTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCG‑TTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACt                  <  1:609061/150‑1 (MQ=255)
                                                                                                                              cTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACt                  >  2:1491887/1‑150 (MQ=255)
                                                                                                                              cTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACt                  <  1:269952/150‑1 (MQ=255)
                                                                                                                              cTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACt                  <  2:2058520/150‑1 (MQ=255)
                                                                                                                              cTCGGCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACt                  <  2:2480504/150‑1 (MQ=255)
                                                                                                                                 ggCTTCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGcc               >  1:3301977/1‑150 (MQ=255)
                                                                                                                                    ttCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATc            <  1:313375/150‑1 (MQ=255)
                                                                                                                                     tCACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCa           <  2:3215083/150‑1 (MQ=255)
                                                                                                                                      cACTGTGTGTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCAc          <  2:2089427/150‑1 (MQ=255)
                                                                                                                                         tgtgtgTTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCaa       >  2:3497947/1‑150 (MQ=255)
                                                                                                                                             tgtTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTc   <  1:3254052/150‑1 (MQ=255)
                                                                                                                                              gtTTAGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCg  >  2:3007071/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AATGGAACGATCAGAGGCAACTGACGCGCGACCAACCAGCGGCACCGCACTTGCCCCTTTCACCGGAGCAAAAGATTGCGGTTTAAAGAAGACGAAAGACGGGTTCTGTTCCAGCAGCTCGCGAACCTCGGCTTCACTGTGTGTTTCGCCCCAGTGACGAATCGCCTGCATCGACATATCTTCTTTTTTCACTTCGCCACGGTCGATCAGCACCTTACCAATGCTGCGATAGGCATGACCGTTTTTCCCTGCATAGCTGAAAAAGTTAAGCGGACTGCCATCACCAAAGTCG  >  CP009273/2939686‑2939977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑