Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 25,546 G→T D114Y (GAC→TAC)  lspA → prolipoprotein signal peptidase (signal peptidase II)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927325,5460GT100.0% 701.6 / NA 214D114Y (GAC→TAC) lspAprolipoprotein signal peptidase (signal peptidase II)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (101/113);  total (101/113)

Only 100 of 214 total aligned reads displayed.

GGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAA  >  CP009273/25399‑25692
                                                                                                                                                   |                                                                                                                                                  
ggcggcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTAc                                                                                                                                                  >  2:722594/1‑150 (MQ=255)
ggcggcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTAc                                                                                                                                                  >  1:1678557/1‑150 (MQ=255)
      tGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCtg                                                                                                                                            >  1:3764666/1‑150 (MQ=255)
          aGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCTACCGCCTGTGGc                                                                                                                                        >  1:2054807/1‑150 (MQ=255)
          aGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGc                                                                                                                                        >  1:2559650/1‑150 (MQ=255)
          aGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGc                                                                                                                                        <  1:1985092/150‑1 (MQ=255)
            cGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCAc                                                                                                                                      <  1:2512459/150‑1 (MQ=255)
            cGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCAc                                                                                                                                      >  2:283981/1‑150 (MQ=255)
             gTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACg                                                                                                                                     >  2:3629687/1‑150 (MQ=255)
                 gTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCtt                                                                                                                                 <  1:677144/150‑1 (MQ=255)
                  ttCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTc                                                                                                                                <  1:1690793/150‑1 (MQ=255)
                   tCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCg                                                                                                                               >  1:1862519/1‑150 (MQ=255)
                      ttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTg                                                                                                                            <  1:1115060/150‑1 (MQ=255)
                       tGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGt                                                                                                                           >  1:3599113/1‑150 (MQ=255)
                        gCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTc                                                                                                                          <  2:42249/150‑1 (MQ=255)
                          cGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGa                                                                                                                        <  1:2654300/150‑1 (MQ=255)
                           ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGat                                                                                                                       >  1:2635576/1‑150 (MQ=255)
                                 gCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCCTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATc                                                                                                                 <  2:373389/150‑1 (MQ=255)
                                   gATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGa                                                                                                               >  2:2209115/1‑150 (MQ=255)
                                    aTTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGAc                                                                                                              >  1:180575/1‑150 (MQ=255)
                                    aTTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGAc                                                                                                              >  1:2302493/1‑150 (MQ=255)
                                     ttGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACt                                                                                                             <  2:1735587/150‑1 (MQ=255)
                                      tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACtt                                                                                                            <  2:2179690/150‑1 (MQ=255)
                                       ggTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTc                                                                                                           >  1:2388174/1‑150 (MQ=255)
                                        gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCt                                                                                                          >  1:899608/1‑150 (MQ=255)
                                          aTTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTAc                                                                                                        <  2:2565712/150‑1 (MQ=255)
                                           ttAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATCTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACg                                                                                                       <  2:629352/150‑1 (MQ=255)
                                                gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGTTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGc                                                                                                  <  2:2340847/150‑1 (MQ=255)
                                                gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGc                                                                                                  >  2:2282670/1‑150 (MQ=255)
                                                gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGc                                                                                                  >  2:1963970/1‑150 (MQ=255)
                                                 tGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTTGGCg                                                                                                 <  1:2797773/150‑1 (MQ=255)
                                                 tGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCg                                                                                                 <  2:1622235/150‑1 (MQ=255)
                                                   aTCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGAc                                                                                               <  1:1505606/150‑1 (MQ=255)
                                                   aTCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGAc                                                                                               >  1:3397871/1‑150 (MQ=255)
                                                    tCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACt                                                                                              <  2:2043466/150‑1 (MQ=255)
                                                     ccTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTg                                                                                             >  2:2745569/1‑150 (MQ=255)
                                                      cTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTgg                                                                                            >  2:602505/1‑150 (MQ=255)
                                                          cAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACt                                                                                        <  2:2236251/150‑1 (MQ=255)
                                                          cAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACt                                                                                        >  2:3493705/1‑150 (MQ=255)
                                                           aGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACtt                                                                                       >  2:1373897/1‑150 (MQ=255)
                                                             tgatgaTGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCg                                                                                     <  1:2411265/150‑1 (MQ=255)
                                                               atgatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGcc                                                                                   >  1:2364840/1‑150 (MQ=255)
                                                                  atgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCAcc                                                                                <  1:1782882/150‑1 (MQ=255)
                                                                   tgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCt                                                                               >  2:2406964/1‑150 (MQ=255)
                                                                    gTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCtt                                                                              <  2:1167177/150‑1 (MQ=255)
                                                                     tATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTc                                                                             <  2:2635576/150‑1 (MQ=255)
                                                                      aTCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCa                                                                            <  2:3235809/150‑1 (MQ=255)
                                                                        cGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAAc                                                                          >  2:1312136/1‑150 (MQ=255)
                                                                          cTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCt                                                                        <  1:1331277/150‑1 (MQ=255)
                                                                           tCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCtt                                                                       >  2:881892/1‑150 (MQ=255)
                                                                            cGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTg                                                                      >  2:3787458/1‑150 (MQ=255)
                                                                                ggCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGa                                                                  >  2:2942059/1‑150 (MQ=255)
                                                                                  ccACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATa                                                                >  2:2896719/1‑150 (MQ=255)
                                                                                  ccACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATa                                                                >  2:2896713/1‑150 (MQ=255)
                                                                                  ccACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATa                                                                >  1:3057688/1‑150 (MQ=255)
                                                                                    aCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACt                                                              <  2:1928402/150‑1 (MQ=255)
                                                                                        aGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCa                                                          >  2:3742367/1‑150 (MQ=255)
                                                                                           aGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCt                                                       <  1:507969/150‑1 (MQ=255)
                                                                                           aGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCt                                                       >  1:1839256/1‑150 (MQ=255)
                                                                                             cTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgt                                                     <  1:2140986/150‑1 (MQ=255)
                                                                                             cTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgt                                                     <  1:2707386/150‑1 (MQ=255)
                                                                                                aaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTc                                                  <  1:3484487/150‑1 (MQ=255)
                                                                                                 aCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCg                                                 >  1:40137/1‑150 (MQ=255)
                                                                                                   aaTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGt                                               >  2:286305/1‑150 (MQ=255)
                                                                                                   aaTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGAGTCGGt                                               >  2:1515407/1‑150 (MQ=255)
                                                                                                    atatCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTg                                              <  1:722594/150‑1 (MQ=255)
                                                                                                    atatCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTg                                              >  1:143823/1‑150 (MQ=255)
                                                                                                    atatCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTg                                              >  2:1006015/1‑150 (MQ=255)
                                                                                                    atatCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTg                                              >  2:1005638/1‑150 (MQ=255)
                                                                                                           ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACt                                       <  1:3628526/150‑1 (MQ=255)
                                                                                                            tACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTg                                      >  2:1851277/1‑150 (MQ=255)
                                                                                                               gcgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAtt                                   <  1:2946401/150‑1 (MQ=255)
                                                                                                               gcgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAtt                                   <  1:1006433/150‑1 (MQ=255)
                                                                                                                  cTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                                <  1:3215736/150‑1 (MQ=255)
                                                                                                                   tGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGc                               <  1:3561898/150‑1 (MQ=255)
                                                                                                                       tattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGa                           >  2:1028350/1‑150 (MQ=255)
                                                                                                                       tattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGa                           <  1:142510/150‑1 (MQ=255)
                                                                                                                         ttGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAg                         <  1:351047/150‑1 (MQ=255)
                                                                                                                            gcggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtt                      <  2:1596471/150‑1 (MQ=255)
                                                                                                                            gcggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtt                      <  2:404355/150‑1 (MQ=255)
                                                                                                                             cggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttt                     >  1:547122/1‑150 (MQ=255)
                                                                                                                               gcgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttttt                   <  2:2375363/150‑1 (MQ=255)
                                                                                                                               gcgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttttt                   <  1:2209012/150‑1 (MQ=255)
                                                                                                                               gcgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttttt                   <  1:2209130/150‑1 (MQ=255)
                                                                                                                                  cgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGc                >  2:2509744/1‑150 (MQ=255)
                                                                                                                                    cTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCt              <  2:899590/150‑1 (MQ=255)
                                                                                                                                    cTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCt              <  1:895927/150‑1 (MQ=255)
                                                                                                                                     tGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGTAGGTTTTTTGCCtt             <  1:1334602/150‑1 (MQ=255)
                                                                                                                                      gggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTc            >  1:2511696/1‑150 (MQ=255)
                                                                                                                                       ggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCt           <  1:1016610/150‑1 (MQ=255)
                                                                                                                                         cAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTag         <  2:180575/150‑1 (MQ=255)
                                                                                                                                         cAACCTGTTCTACCGCCTGTGGCACGGCGTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTag         <  2:2302493/150‑1 (MQ=255)
                                                                                                                                          aaCCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTaga        >  1:1584496/1‑150 (MQ=255)
                                                                                                                                          aaCCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTaga        <  1:1296005/150‑1 (MQ=255)
                                                                                                                                               gTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGaa   <  2:2974721/150‑1 (MQ=255)
                                                                                                                                               gTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGaa   >  2:3562054/1‑150 (MQ=255)
                                                                                                                                               gTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGaa   >  2:1554299/1‑150 (MQ=255)
                                                                                                                                                ttCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGaaa  <  1:494298/150‑1 (MQ=255)
                                                                                                                                                ttCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGaaa  <  1:2896719/150‑1 (MQ=255)
                                                                                                                                                ttCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGaaa  <  1:2896713/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCGGCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAA  >  CP009273/25399‑25692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑