BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,065,123	C→T	G287S (GGT→AGT)	pgk ←	phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,065,123	0	C	T	99.5%	651.8 / NA	196	G287S (GGT→AGT)	pgk	phosphoglycerate kinase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (91/104); total (91/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

AGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCA  >  CP009273/3064976‑3065269

agaACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGa                                                                                                                                                  >  1:614119/1‑150 (MQ=255)

 gaACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGATTGGTTTTCGCATTCTTCAGGATTTCTGCCAGTTCCTGAGCGGAAGCATCACTGat                                                                                                                                                 <  2:123783/150‑1 (MQ=255)

   aCGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGatat                                                                                                                                               >  2:818511/1‑150 (MQ=255)

    cGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATc                                                                                                                                              >  2:1348295/1‑150 (MQ=255)

     gCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATcc                                                                                                                                             <  2:575968/150‑1 (MQ=255)

     gCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATcc                                                                                                                                             <  2:2294869/150‑1 (MQ=255)

      cTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGGATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCa                                                                                                                                            >  2:6168/1‑150 (MQ=255)

       ttCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAg                                                                                                                                           <  1:3747702/150‑1 (MQ=255)

        tCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAgg                                                                                                                                          <  2:792170/150‑1 (MQ=255)

           cTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATc                                                                                                                                       <  2:938020/150‑1 (MQ=255)

            tGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCt                                                                                                                                      >  2:2849687/1‑150 (MQ=255)

            tGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCt                                                                                                                                      <  2:162861/150‑1 (MQ=255)

             gTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTg                                                                                                                                     <  1:945980/150‑1 (MQ=255)

             gTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTg                                                                                                                                     >  1:2469497/1‑150 (MQ=255)

              tCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGc                                                                                                                                    >  1:121541/1‑150 (MQ=255)

                tGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGATGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTc                                                                                                                                  <  2:2421495/150‑1 (MQ=255)

                    aTAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCa                                                                                                                              <  2:411415/150‑1 (MQ=255)

                       gCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCt                                                                                                                           <  1:2656268/150‑1 (MQ=255)

                         gTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCttt                                                                                                                         <  1:1344473/150‑1 (MQ=255)

                          ttAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTc                                                                                                                        >  1:1310412/1‑150 (MQ=255)

                            aGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTcac                                                                                                                      <  2:2348489/150‑1 (MQ=255)

                             gCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTcaca                                                                                                                     >  2:2468984/1‑150 (MQ=255)

                                   aTTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTa                                                                                                               >  1:1104237/1‑150 (MQ=255)

                                    tttCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTaa                                                                                                              >  2:1132586/1‑150 (MQ=255)

                                    tttCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTaa                                                                                                              >  2:671700/1‑150 (MQ=255)

                                          tACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGAtt                                                                                                        <  2:2776528/150‑1 (MQ=255)

                                           aCCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGAttt                                                                                                       <  2:2555101/150‑1 (MQ=255)

                                            ccTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTc                                                                                                      <  1:2404593/150‑1 (MQ=255)

                                               ttGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAgg                                                                                                   <  2:1950036/150‑1 (MQ=255)

                                                 gCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGt                                                                                                 <  2:3670347/150‑1 (MQ=255)

                                                  cGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTa                                                                                                <  1:1081456/150‑1 (MQ=255)

                                                  cGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTa                                                                                                <  2:1133593/150‑1 (MQ=255)

                                                   ggAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAg                                                                                               <  1:1691852/150‑1 (MQ=255)

                                                   ggAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAg                                                                                               >  2:1304212/1‑150 (MQ=255)

                                                     aaGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGcc                                                                                             <  2:2692768/150‑1 (MQ=255)

                                                      aGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCg                                                                                            >  2:1171393/1‑150 (MQ=255)

                                                        ttCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGt                                                                                          >  2:1478663/1‑150 (MQ=255)

                                                         tCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTg                                                                                         <  1:1631110/150‑1 (MQ=255)

                                                         tCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTg                                                                                         >  1:89483/1‑150 (MQ=255)

                                                           gggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCa                                                                                       >  1:3571963/1‑150 (MQ=255)

                                                           gggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCa                                                                                       <  2:1390516/150‑1 (MQ=255)

                                                           gggAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCa                                                                                       <  2:2920399/150‑1 (MQ=255)

                                                             gAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGt                                                                                     >  1:668916/1‑150 (MQ=255)

                                                                  cNAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCag                                                                                >  1:1637994/1‑150 (MQ=255)

                                                                  cGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCag                                                                                >  2:3714276/1‑150 (MQ=255)

                                                                  cGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCag                                                                                >  2:3641631/1‑150 (MQ=255)

                                                                   gAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCaga                                                                               >  2:183781/1‑150 (MQ=255)

                                                                   gAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCaga                                                                               <  2:3073673/150‑1 (MQ=255)

                                                                    aaCACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCagag                                                                              <  1:3332348/150‑1 (MQ=255)

                                                                    aaCACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCagag                                                                              >  1:1929739/1‑150 (MQ=255)

                                                                     acacGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAgaga                                                                             >  1:3497699/1‑150 (MQ=255)

                                                                        cGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACt                                                                          <  2:1595731/150‑1 (MQ=255)

                                                                         gCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTc                                                                         >  1:3451029/1‑150 (MQ=255)

                                                                          ccAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCg                                                                        >  1:2896270/1‑150 (MQ=255)

                                                                           cAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACAGCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCgg                                                                       >  2:2106344/1‑150 (MQ=255)

                                                                              ccGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTg                                                                    >  1:3222012/1‑150 (MQ=255)

                                                                               cGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGc                                                                   >  2:3397759/1‑150 (MQ=255)

                                                                                 gACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTa                                                                 >  1:218637/1‑150 (MQ=255)

                                                                                  aCCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTAc                                                                >  1:885554/1‑150 (MQ=255)

                                                                                    cGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTAcgc                                                              >  1:3691978/1‑150 (MQ=255)

                                                                                      ttCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGa                                                            >  2:207087/1‑150 (MQ=255)

                                                                                        ccACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAAc                                                          <  2:2040177/150‑1 (MQ=255)

                                                                                         cacaGAATGGTTTTCGCATTCGTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACa                                                         <  2:1280800/150‑1 (MQ=255)

                                                                                            aGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCa                                                      >  2:2803696/1‑150 (MQ=255)

                                                                                               aTGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAc                                                   <  1:1349427/150‑1 (MQ=255)

                                                                                               aTGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGAc                                                   <  1:1132586/150‑1 (MQ=255)

                                                                                                 ggTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACgg                                                 >  1:2001963/1‑150 (MQ=255)

                                                                                                   ttttCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGaa                                               >  1:2134732/1‑150 (MQ=255)

                                                                                                     ttCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGATACGCGAACATCAGACGGAAcc                                             <  1:2849687/150‑1 (MQ=255)

                                                                                                      tCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGACCCg                                            >  2:1764889/1‑150 (MQ=255)

                                                                                                       cGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCgg                                           <  1:1447864/150‑1 (MQ=255)

                                                                                                        gCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCggg                                          >  1:2359941/1‑150 (MQ=255)

                                                                                                           ttcttcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATg                                       >  1:844611/1‑150 (MQ=255)

                                                                                                             cttcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGtt                                     <  2:1024601/150‑1 (MQ=255)

                                                                                                               tcAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGc                                   <  1:3011678/150‑1 (MQ=255)

                                                                                                                cAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCa                                  >  2:258173/1‑150 (MQ=255)

                                                                                                                  ggATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAgg                                <  2:1444420/150‑1 (MQ=255)

                                                                                                                  ggATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAgg                                <  1:3641631/150‑1 (MQ=255)

                                                                                                                  ggATTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAgg                                <  1:3714276/150‑1 (MQ=255)

                                                                                                                    aTTTCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAggtg                              >  2:1757118/1‑150 (MQ=255)

                                                                                                                     tttCAGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAggtgg                             >  1:113949/1‑150 (MQ=255)

                                                                                                                         aGCCAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcag                         >  1:2746938/1‑150 (MQ=255)

                                                                                                                           ccAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcagca                       <  1:1478663/150‑1 (MQ=255)

                                                                                                                           ccAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcagca                       <  1:3424871/150‑1 (MQ=255)

                                                                                                                           ccAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcagca                       >  2:2125333/1‑150 (MQ=255)

                                                                                                                            cAGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTcagcag                      >  2:3511788/1‑150 (MQ=255)

                                                                                                                             aGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGa                     >  1:2729338/1‑150 (MQ=255)

                                                                                                                             aGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGa                     >  2:3776688/1‑150 (MQ=255)

                                                                                                                             aGTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGa                     >  1:1397755/1‑150 (MQ=255)

                                                                                                                              gTTCCTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGAc                    <  2:3545869/150‑1 (MQ=255)

                                                                                                                                  cTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGttt                <  2:536722/150‑1 (MQ=255)

                                                                                                                                  cTGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGttt                <  1:818511/150‑1 (MQ=255)

                                                                                                                                   tGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTa               >  2:3523687/1‑150 (MQ=255)

                                                                                                                                   tGAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTa               >  1:3730509/1‑150 (MQ=255)

                                                                                                                                    gAGCGGAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAg              <  2:3298367/150‑1 (MQ=255)

                                                                                                                                        ggAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTc          <  1:576900/150‑1 (MQ=255)

                                                                                                                                        ggAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTc          <  2:1929739/150‑1 (MQ=255)

                                                                                                                                         gAAGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCg         >  1:529627/1‑150 (MQ=255)

                                                                                                                                          aaGCATCACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGt        >  1:359046/1‑150 (MQ=255)

                                                                                                                                                cACTGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCa  >  1:410802/1‑150 (MQ=255)

AGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGTTCCACAGAATGGTTTTCGCATTCTTCAGGATTTCAGCCAGTTCCTGAGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCA  >  CP009273/3064976‑3065269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`