Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,088,065 C→A V125V (GTG→GTT)  yggR ← putative PilT family AAA+ ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,088,0650CA99.5% 642.0 / NA 193V125V (GTG→GTT) yggRputative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (114/78);  minor base T (0/1);  total (114/79)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

TTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATCG  >  CP009273/3087918‑3088211
                                                                                                                                                   |                                                                                                                                                  
ttCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAAc                                                                                                                                                  >  2:3659440/1‑150 (MQ=255)
ttCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAAc                                                                                                                                                  >  2:2336831/1‑150 (MQ=255)
  cccGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATAGTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCa                                                                                                                                                <  2:768899/150‑1 (MQ=255)
   ccGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAg                                                                                                                                               <  2:2907250/150‑1 (MQ=255)
     gctgctGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGaa                                                                                                                                             >  2:855844/1‑150 (MQ=255)
      ctgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAAt                                                                                                                                            >  1:386329/1‑150 (MQ=255)
      ctgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAAt                                                                                                                                            <  1:1395115/150‑1 (MQ=255)
      ctgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAAt                                                                                                                                            >  1:1843177/1‑150 (MQ=255)
       tgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATc                                                                                                                                           >  2:163131/1‑150 (MQ=255)
       tgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATc                                                                                                                                           <  2:42835/150‑1 (MQ=255)
       tgctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATc                                                                                                                                           <  1:976439/150‑1 (MQ=255)
         ctgGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAg                                                                                                                                         >  2:1016367/1‑150 (MQ=255)
             aTCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCa                                                                                                                                     <  2:748384/150‑1 (MQ=255)
              tCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAt                                                                                                                                    >  1:2203149/1‑150 (MQ=255)
               cAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAtt                                                                                                                                   >  2:2099687/1‑150 (MQ=255)
                aaaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATtc                                                                                                                                  >  1:789360/1‑150 (MQ=255)
                aaaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATtc                                                                                                                                  >  2:368503/1‑150 (MQ=255)
                aaaCATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATtc                                                                                                                                  <  2:432055/150‑1 (MQ=255)
                    aTCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGc                                                                                                                              >  1:33445/1‑150 (MQ=255)
                         tggctggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTg                                                                                                                         >  2:1323420/1‑150 (MQ=255)
                            ctggcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGt                                                                                                                      >  2:1776625/1‑150 (MQ=255)
                               gcATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAAt                                                                                                                   <  2:241853/150‑1 (MQ=255)
                                cATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAAtt                                                                                                                  <  2:1626188/150‑1 (MQ=255)
                                cATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAAtt                                                                                                                  <  2:3521603/150‑1 (MQ=255)
                                 aTAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTc                                                                                                                 >  2:2827561/1‑150 (MQ=255)
                                 aTAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTc                                                                                                                 >  2:1835413/1‑150 (MQ=255)
                                        tatTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAAt                                                                                                          >  1:2390860/1‑150 (MQ=255)
                                         atTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATa                                                                                                         <  2:3578564/150‑1 (MQ=255)
                                         atTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATa                                                                                                         <  2:2073290/150‑1 (MQ=255)
                                            cAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTTACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCg                                                                                                      <  1:3590421/150‑1 (MQ=255)
                                             aaCAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGt                                                                                                     >  2:356307/1‑150 (MQ=255)
                                             aaCAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGt                                                                                                     >  2:176047/1‑150 (MQ=255)
                                             aaCAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGt                                                                                                     >  2:223431/1‑150 (MQ=255)
                                               cAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTg                                                                                                   <  1:290838/150‑1 (MQ=255)
                                               cAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTg                                                                                                   <  1:218530/150‑1 (MQ=255)
                                                   aTCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTgc                                                                                               >  2:2248896/1‑150 (MQ=255)
                                                    tCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTgcg                                                                                              <  2:876614/150‑1 (MQ=255)
                                                    tCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTgcg                                                                                              <  1:1016367/150‑1 (MQ=255)
                                                    tCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAAGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTgcg                                                                                              <  1:1614293/150‑1 (MQ=255)
                                                       tCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCa                                                                                           <  1:2015711/150‑1 (MQ=255)
                                                        ccAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCACTACCGTTGGTGCGCCaa                                                                                          >  1:2428898/1‑150 (MQ=255)
                                                          aGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGc                                                                                        >  1:1420032/1‑150 (MQ=255)
                                                           gCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCt                                                                                       <  1:3142410/150‑1 (MQ=255)
                                                            cGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTg                                                                                      >  2:1843224/1‑150 (MQ=255)
                                                                aGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCATCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCg                                                                                  >  1:3431566/1‑150 (MQ=255)
                                                                aGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCg                                                                                  >  2:545923/1‑150 (MQ=255)
                                                                  aaTATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAg                                                                                >  2:818064/1‑150 (MQ=255)
                                                                   atatGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGc                                                                               >  2:1964300/1‑150 (MQ=255)
                                                                     atGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTg                                                                             <  1:163131/150‑1 (MQ=255)
                                                                              ggCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTg                                                                    <  1:3678572/150‑1 (MQ=255)
                                                                               gCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGc                                                                   <  2:2650072/150‑1 (MQ=255)
                                                                               gCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGc                                                                   >  1:2118797/1‑150 (MQ=255)
                                                                                cATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCg                                                                  >  2:1895902/1‑150 (MQ=255)
                                                                                 aTGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGa                                                                 >  1:1758883/1‑150 (MQ=255)
                                                                                 aTGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGa                                                                 >  1:2881244/1‑150 (MQ=255)
                                                                                 aTGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGa                                                                 >  2:2654500/1‑150 (MQ=255)
                                                                                    ttgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAGTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAgg                                                              <  2:1480691/150‑1 (MQ=255)
                                                                                     tgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGt                                                             <  1:3524488/150‑1 (MQ=255)
                                                                                     tgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGt                                                             >  1:712837/1‑150 (MQ=255)
                                                                                     tgattgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGt                                                             >  1:294381/1‑150 (MQ=255)
                                                                                       attgaGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTaa                                                           >  1:977918/1‑150 (MQ=255)
                                                                                          gagaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAg                                                        >  2:3729797/1‑150 (MQ=255)
                                                                                          gagaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAg                                                        >  1:445795/1‑150 (MQ=255)
                                                                                           agaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGc                                                       <  2:386329/150‑1 (MQ=255)
                                                                                            gaTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGcc                                                      <  1:2784727/150‑1 (MQ=255)
                                                                                             aTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCg                                                     >  1:1707789/1‑150 (MQ=255)
                                                                                             aTAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCg                                                     >  1:866348/1‑150 (MQ=255)
                                                                                               aGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTa                                                   <  1:1928011/150‑1 (MQ=255)
                                                                                                gCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTaa                                                  >  2:726636/1‑150 (MQ=255)
                                                                                                  cAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACg                                                >  2:3231125/1‑150 (MQ=255)
                                                                                                   aaCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACga                                               >  2:350214/1‑149 (MQ=255)
                                                                                                    aCCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGcc                                              <  2:3207165/150‑1 (MQ=255)
                                                                                                     ccATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCAGTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCa                                             <  2:2905189/150‑1 (MQ=255)
                                                                                                       aTCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGc                                           <  1:1189127/150‑1 (MQ=255)
                                                                                                         cgccgcCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGa                                         >  2:2372470/1‑150 (MQ=255)
                                                                                                           ccgccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGaaa                                       <  1:572198/150‑1 (MQ=255)
                                                                                                            cgccAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAat                                      <  2:670540/150‑1 (MQ=255)
                                                                                                               cAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcc                                   <  1:2249090/150‑1 (MQ=255)
                                                                                                                aGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgcca                                  >  1:2407970/1‑150 (MQ=255)
                                                                                                                  cGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatg                                <  2:33445/150‑1 (MQ=255)
                                                                                                                   gTGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatgc                               >  2:334955/1‑150 (MQ=255)
                                                                                                                    tGGTAGATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAatgccatgcc                              <  1:632027/150‑1 (MQ=255)
                                                                                                                         gATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCGAGCGAAATGCCATGCCGTTgc                         <  2:1440950/150‑1 (MQ=255)
                                                                                                                         gATTTGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTgc                         >  1:2510180/1‑150 (MQ=255)
                                                                                                                             tGCCACTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGa                     <  2:1707789/150‑1 (MQ=255)
                                                                                                                                 aCTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATgc                 >  1:500389/1‑150 (MQ=255)
                                                                                                                                 aCTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATgc                 >  1:500491/1‑150 (MQ=255)
                                                                                                                                  cTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATgcg                <  1:2553300/150‑1 (MQ=255)
                                                                                                                                  cTCCCCGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATgcg                >  2:2190947/1‑150 (MQ=255)
                                                                                                                                     cccGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTg             >  2:3029464/1‑150 (MQ=255)
                                                                                                                                     cccGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTg             >  2:2126196/1‑150 (MQ=255)
                                                                                                                                     cccGTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTg             >  2:2126175/1‑150 (MQ=255)
                                                                                                                                        gTCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCa          <  2:1226376/150‑1 (MQ=255)
                                                                                                                                         tCGCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTACCAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCAc         >  2:1048277/1‑150 (MQ=255)
                                                                                                                                           gCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGc       <  2:3135601/150‑1 (MQ=255)
                                                                                                                                           gCCCCCGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAAGTCCGGCAATACCGTTGGTGCGCCAAACTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGc       <  2:3251559/150‑1 (MQ=255)
                                                                                                                                             ccccGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCaa     <  1:1245205/150‑1 (MQ=255)
                                                                                                                                              cccGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAAt    <  2:1095549/150‑1 (MQ=255)
                                                                                                                                               ccGTAACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTAGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATc   >  1:1103751/1‑150 (MQ=255)
                                                                                                                                               ccGTAACCAGAATCAGGCCATTCACGCTCTTGAGTAATTCCGGCAATACCGTTGGAGCGCCAAGCAGTTCGAGCTGAGGGCAGTGCGAAGGAAACAGCCGAAACGCCAGCGAAATGCCATGCCGATGCGCGAATTCGCTGCCACGCAATc   >  1:3195878/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCCCGCTGCTGGATCAAACATCGCTGGCTGGCATAGAGATATTCAACAGGATCTTCCAGCGTCAGAATATGCGCATCGGCATGTTGATTGAGATAGCCAACCATCGCCGCCAGCGTGGTAGATTTGCCACTCCCCGTCGCCCCCGTCACCAGAATCAGGCCATTCTCGCTCTTGAGTAATTCCGGCAATACCGTTGGTGCGCCAAGCTGTTCGAGCTGCGGGCAGTGCGAAGGTAACAGCCGTAACGCCAGCGAAATGCCATGCCGTTGCGCGAATGCGCTGCCACGCAATCG  >  CP009273/3087918‑3088211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑