Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,116,446 A→C L240R (CTG→CGG)  glcB ← malate synthase G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,116,4460AC99.6% 941.5 / ‑4.8 242L240R (CTG→CGG) glcBmalate synthase G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (112/129);  minor base T (1/0);  total (113/129)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 242 total aligned reads displayed.

TGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTA  >  CP009273/3116300‑3116592
                                                                                                                                                  |                                                                                                                                                  
tGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCt                                                                                                                                                 <  2:2437540/150‑1 (MQ=255)
   ggTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCaa                                                                                                                                              <  2:2887464/150‑1 (MQ=255)
    gTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAat                                                                                                                                             >  2:2334521/1‑150 (MQ=255)
       cagcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATg                                                                                                                                          >  1:2106677/1‑150 (MQ=255)
       cagcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATg                                                                                                                                          >  2:1149130/1‑150 (MQ=255)
       cagcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATg                                                                                                                                          >  1:1361173/1‑150 (MQ=255)
       cagcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATg                                                                                                                                          <  2:654286/150‑1 (MQ=255)
        agcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGc                                                                                                                                         >  1:1850909/1‑150 (MQ=255)
         gcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCa                                                                                                                                        <  2:1735599/150‑1 (MQ=255)
         gcagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCa                                                                                                                                        <  2:3629689/150‑1 (MQ=255)
          cagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAg                                                                                                                                       <  1:863927/150‑1 (MQ=255)
          cagGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAg                                                                                                                                       >  2:1105974/1‑150 (MQ=255)
           agGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAgg                                                                                                                                      >  2:143424/1‑150 (MQ=255)
            ggATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGc                                                                                                                                     >  2:2495219/1‑150 (MQ=255)
                tttATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGtt                                                                                                                                 <  1:2194015/150‑1 (MQ=255)
                tttATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGtt                                                                                                                                 >  1:2906146/1‑150 (MQ=255)
                tttATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCAGGATCGTCTTTGCCAATCCGCCCATTGGCATATATTTGCCGCTCAATATGCAGGCCGtt                                                                                                                                 <  1:3713503/150‑1 (MQ=255)
                  tATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTAt                                                                                                                               >  2:2359789/1‑150 (MQ=255)
                  tATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTAt                                                                                                                               >  2:3068201/1‑150 (MQ=255)
                   aTCGTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCATGCCGTTAtt                                                                                                                              <  1:3794228/150‑1 (MQ=255)
                       tCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTc                                                                                                                          <  2:11023/150‑1 (MQ=255)
                       tCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTc                                                                                                                          <  2:936495/150‑1 (MQ=255)
                             tCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCaaa                                                                                                                    <  2:1896531/150‑1 (MQ=255)
                               aaCCGCCGCGACCGAATCTTCGCAGTCGAGTATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGGAGGCCGTTATTTTTCAGCAAAAt                                                                                                                  <  2:2186951/150‑1 (MQ=255)
                                   gccgcGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAg                                                                                                              >  1:2814283/1‑150 (MQ=255)
                                    ccgcGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAgg                                                                                                             <  1:2042820/150‑1 (MQ=255)
                                     cgcgACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGt                                                                                                            >  1:1240194/1‑150 (MQ=255)
                                     cgcgACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGt                                                                                                            <  1:2558202/150‑1 (MQ=255)
                                        gACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGACAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATTCAGGTctg                                                                                                         >  2:2285651/1‑148 (MQ=255)
                                           cGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGgcgc                                                                                                      >  1:3548288/1‑150 (MQ=255)
                                            gAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCa                                                                                                     >  1:1596063/1‑150 (MQ=255)
                                            gAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCa                                                                                                     >  1:589214/1‑150 (MQ=255)
                                            gAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCa                                                                                                     <  2:120222/150‑1 (MQ=255)
                                              aTCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGc                                                                                                   >  1:2376274/1‑150 (MQ=255)
                                              aTCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGc                                                                                                   >  1:1841574/1‑150 (MQ=255)
                                                cTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCgg                                                                                                 <  2:2221683/150‑1 (MQ=255)
                                                  tCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCa                                                                                               >  2:3394088/1‑150 (MQ=255)
                                                   cGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCTATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCAt                                                                                              >  1:1499872/1‑150 (MQ=255)
                                                     cAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCg                                                                                            <  1:1275020/150‑1 (MQ=255)
                                                      aGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGc                                                                                           >  1:2294856/1‑150 (MQ=255)
                                                        tCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCa                                                                                         <  2:2132758/150‑1 (MQ=255)
                                                         cGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCAc                                                                                        <  1:2495982/150‑1 (MQ=255)
                                                            gaATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGt                                                                                     >  2:316002/1‑150 (MQ=255)
                                                             aaTGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTa                                                                                    <  2:505845/150‑1 (MQ=255)
                                                               tGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAAc                                                                                  <  1:695226/150‑1 (MQ=255)
                                                                ggTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAAcc                                                                                 <  2:772133/150‑1 (MQ=255)
                                                                ggTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAAcc                                                                                 <  2:3362512/150‑1 (MQ=255)
                                                                 gTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCg                                                                                <  1:414064/150‑1 (MQ=255)
                                                                      gATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACaaa                                                                           <  1:3189446/150‑1 (MQ=255)
                                                                       aTAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAAc                                                                          >  2:2918681/1‑150 (MQ=255)
                                                                         agcagcTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACtg                                                                        >  2:3499745/1‑150 (MQ=255)
                                                                           cagcTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACtgtg                                                                      <  1:2580100/150‑1 (MQ=255)
                                                                                tCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGa                                                                 <  1:2805406/150‑1 (MQ=255)
                                                                                 cgacgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAg                                                                <  2:3307634/150‑1 (MQ=255)
                                                                                  gacgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGt                                                               <  1:3632926/150‑1 (MQ=255)
                                                                                   acgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTa                                                              >  2:1282492/1‑150 (MQ=255)
                                                                                    cgaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTAc                                                             <  1:429431/150‑1 (MQ=255)
                                                                                     gaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACg                                                            <  1:2067998/150‑1 (MQ=255)
                                                                                     gaTAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACg                                                            <  1:1084677/150‑1 (MQ=255)
                                                                                        aaCATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTaa                                                         <  2:1512984/150‑1 (MQ=255)
                                                                                        aaCATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTaa                                                         <  2:589214/150‑1 (MQ=255)
                                                                                           aTCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGt                                                      <  2:2568478/150‑1 (MQ=255)
                                                                                           aTCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGt                                                      >  2:2020053/1‑150 (MQ=255)
                                                                                            tCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTg                                                     <  2:3751093/150‑1 (MQ=255)
                                                                                             cGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTgg                                                    >  1:3367722/1‑150 (MQ=255)
                                                                                             cGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTgg                                                    >  2:2667477/1‑150 (MQ=255)
                                                                                             cGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTgg                                                    >  2:762581/1‑150 (MQ=255)
                                                                                                tGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGACGCCTCGGCATCGCCACTGAAACCGACAAACTGTGCTGGAGTACGTAACGTGGttt                                                 >  1:3005818/1‑150 (MQ=255)
                                                                                                  aTGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCt                                               <  1:2359789/150‑1 (MQ=255)
                                                                                                   tgtgCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCtt                                              <  2:3723113/150‑1 (MQ=255)
                                                                                                     tgCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTa                                            >  1:3782033/1‑150 (MQ=255)
                                                                                                     tgCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTa                                            <  2:1430941/150‑1 (MQ=255)
                                                                                                       cgcCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTAcc                                          <  2:87591/150‑1 (MQ=255)
                                                                                                       cgcCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTAcc                                          >  2:1659158/1‑150 (MQ=255)
                                                                                                           ggATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCAttt                                      >  1:1700790/1‑150 (MQ=255)
                                                                                                            gATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCAtttt                                     >  1:3254429/1‑150 (MQ=255)
                                                                                                               cGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCa                                  >  2:3410745/1‑150 (MQ=255)
                                                                                                               cGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCa                                  >  1:509134/1‑150 (MQ=255)
                                                                                                                gtatttGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCaa                                 <  1:1784306/147‑1 (MQ=255)
                                                                                                                 tCTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAAc                                <  2:1841574/150‑1 (MQ=255)
                                                                                                                  cTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATTCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACt                               <  2:2985996/150‑1 (MQ=255)
                                                                                                                  cTTTGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACt                               >  2:3176899/1‑150 (MQ=255)
                                                                                                                    ttGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTgg                             <  1:3447286/150‑1 (MQ=255)
                                                                                                                     tGCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGa                            >  1:907872/1‑150 (MQ=255)
                                                                                                                      gCCAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGAt                           <  2:3548221/150‑1 (MQ=255)
                                                                                                                       ccAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATg                          <  2:1596063/150‑1 (MQ=255)
                                                                                                                        cAATCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGc                         <  2:1291129/150‑1 (MQ=255)
                                                                                                                           tCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTa                      <  1:3575589/150‑1 (MQ=255)
                                                                                                                           tCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTa                      <  2:156200/150‑1 (MQ=255)
                                                                                                                           tCCGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTa                      <  2:2677987/150‑1 (MQ=255)
                                                                                                                             cGCCCATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAAt                    >  2:281001/1‑150 (MQ=255)
                                                                                                                                 cATTGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGtt                <  1:3068201/150‑1 (MQ=255)
                                                                                                                                   ttGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTa              <  2:24326/150‑1 (MQ=255)
                                                                                                                                   ttGGCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTa              >  2:1791015/1‑150 (MQ=255)
                                                                                                                                     ggCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATc            >  2:2656094/1‑150 (MQ=255)
                                                                                                                                      gCATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCa           <  1:3746160/150‑1 (MQ=255)
                                                                                                                                       cATCGATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCaa          >  1:545312/1‑150 (MQ=255)
                                                                                                                                           gATTTGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAaccac      >  2:1290667/1‑150 (MQ=255)
                                                                                                                                              ttGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCtt   <  1:143424/150‑1 (MQ=255)
                                                                                                                                               tGCCGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTa  >  1:110604/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATGGTACTGATAGCAGCTTCGACGATAACATCGTTGATGTGCGCCGGATCGTCTTTGCCAATCCGCCCATTGGCATCGATTTGCAGCTCAATATGCAGGCCGTTATTTTTCAGCAAAATGCAGGTCGGCGCAGCGGCATCGCCACGGTAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTA  >  CP009273/3116300‑3116592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑