Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,122,356 G→T L242F (TTG→TTT)  glcC → glycolate‑inducible glc operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,122,3560GT99.6% 741.0 / NA 226L242F (TTG→TTT) glcCglycolate‑inducible glc operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (94/131);  minor base A (1/0);  total (95/131)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

GCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGAC  >  CP009273/3122209‑3122501
                                                                                                                                                   |                                                                                                                                                 
gCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTAt                                                                                                                                                 <  2:467087/150‑1 (MQ=255)
gCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTAt                                                                                                                                                 <  2:1547730/150‑1 (MQ=255)
  aaaaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTc                                                                                                                                               <  2:3794131/150‑1 (MQ=255)
  aaaaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTc                                                                                                                                               >  1:571494/1‑150 (MQ=255)
  aaaaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTc                                                                                                                                               <  1:1977028/150‑1 (MQ=255)
  aaaaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTc                                                                                                                                               <  1:1934286/150‑1 (MQ=255)
  aaaaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTc                                                                                                                                               >  2:2560479/1‑150 (MQ=255)
   aaaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCg                                                                                                                                              >  1:2263068/1‑150 (MQ=255)
     aaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCt                                                                                                                                            <  1:3344845/150‑1 (MQ=255)
       aaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcg                                                                                                                                          <  2:3513187/150‑1 (MQ=255)
        aGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcgg                                                                                                                                         <  1:3117396/150‑1 (MQ=255)
         gCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcggc                                                                                                                                        >  1:1147646/1‑150 (MQ=255)
          cAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcggcg                                                                                                                                       >  1:3162051/1‑150 (MQ=255)
          cAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcggcg                                                                                                                                       <  1:3005324/150‑1 (MQ=255)
            gatcgatcGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGt                                                                                                                                     <  2:131250/150‑1 (MQ=255)
             atcgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAATGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTg                                                                                                                                    >  2:439130/1‑150 (MQ=255)
             atcgatcgCCAGCATGCGCGGATCTACAACGCGCAGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGAGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTg                                                                                                                                    >  2:1142584/1‑150 (MQ=255)
               cgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGcc                                                                                                                                  <  2:3595821/150‑1 (MQ=255)
                gatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCg                                                                                                                                 >  2:3331733/1‑150 (MQ=255)
                   cgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTg                                                                                                                              <  2:2991235/150‑1 (MQ=255)
                   cgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTg                                                                                                                              >  2:1047735/1‑150 (MQ=255)
                   cgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTg                                                                                                                              >  1:2451170/1‑150 (MQ=255)
                     ccAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGga                                                                                                                            >  1:901903/1‑150 (MQ=255)
                          aTGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGa                                                                                                                       >  2:1566561/1‑150 (MQ=255)
                           tGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGaa                                                                                                                      <  1:2944922/150‑1 (MQ=255)
                                 gATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAg                                                                                                                >  2:350004/1‑150 (MQ=255)
                                 gATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAg                                                                                                                >  2:3234960/1‑150 (MQ=255)
                                   tCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGtt                                                                                                              >  1:1345126/1‑150 (MQ=255)
                                     tACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAg                                                                                                            <  2:2422662/150‑1 (MQ=255)
                                      aCAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGc                                                                                                           <  1:1029906/150‑1 (MQ=255)
                                        aaCGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTg                                                                                                         >  1:2857883/1‑150 (MQ=255)
                                          cgcgGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGt                                                                                                       >  1:3788181/1‑150 (MQ=255)
                                              gtgtTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTa                                                                                                   <  2:856898/150‑1 (MQ=255)
                                                   gCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTG‑AAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTg                                                                                             <  2:2901434/150‑1 (MQ=255)
                                                      gCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCt                                                                                           <  1:2407228/150‑1 (MQ=255)
                                                       cGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTCTTACATCTGCtt                                                                                          >  2:3784141/1‑150 (MQ=255)
                                                       cGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCtt                                                                                          >  1:935366/1‑150 (MQ=255)
                                                          cTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTc                                                                                       <  2:3049895/150‑1 (MQ=255)
                                                            gCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCAt                                                                                     <  2:161847/150‑1 (MQ=255)
                                                             ccGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATa                                                                                    <  2:3457794/150‑1 (MQ=255)
                                                              cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATAc                                                                                   >  2:561724/1‑150 (MQ=255)
                                                              cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATAc                                                                                   <  1:3620278/150‑1 (MQ=255)
                                                              cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATAc                                                                                   <  2:3177423/150‑1 (MQ=255)
                                                                 aCGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAAt                                                                                <  2:2244975/150‑1 (MQ=255)
                                                                    tCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCgg                                                                             <  1:923056/150‑1 (MQ=255)
                                                                     cGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                            <  2:2618461/150‑1 (MQ=255)
                                                                     cGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                            <  1:1548602/150‑1 (MQ=255)
                                                                      gCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                           >  1:717962/1‑150 (MQ=255)
                                                                         cAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACg                                                                        <  2:1032393/150‑1 (MQ=255)
                                                                          aGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc                                                                       >  1:1039236/1‑150 (MQ=255)
                                                                           gcgcgcAGCTCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCAGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCt                                                                      <  1:1268084/150‑1 (MQ=255)
                                                                           gcgcgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCATCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTTAATCTGCTTTTCATACAATCGGTAACGCt                                                                      <  1:2377764/150‑1 (MQ=255)
                                                                            cgcgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCtt                                                                     <  1:2632049/150‑1 (MQ=255)
                                                                            cgcgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCtt                                                                     >  2:2720126/1‑150 (MQ=255)
                                                                              cgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGt                                                                   <  2:2119601/150‑1 (MQ=255)
                                                                                cagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTAc                                                                 >  1:1224499/1‑150 (MQ=255)
                                                                                 agcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACg                                                                <  1:2152730/150‑1 (MQ=255)
                                                                                       cgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCg                                                          >  2:328053/1‑150 (MQ=255)
                                                                                       cgATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCg                                                          <  2:218840/150‑1 (MQ=255)
                                                                                        gATCATGTGCGGACCATGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGc                                                         >  1:2619920/1‑150 (MQ=255)
                                                                                          tCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGccc                                                       >  2:2813195/1‑150 (MQ=255)
                                                                                            aTGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCa                                                     >  1:402588/1‑150 (MQ=255)
                                                                                              gtgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAAt                                                   <  2:1324789/150‑1 (MQ=255)
                                                                                              gtgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAAt                                                   <  2:3658799/150‑1 (MQ=255)
                                                                                                gCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGc                                                 <  1:971828/150‑1 (MQ=255)
                                                                                                   gACCGTGAAAAAGAATCTCCACGATATAGAGCTGGAAGGCCACCATTTTATTCTCTCGGCGGTGCCACTTGAGAAGAACATCAGATAGCTGGTATTAAATCTGCTTTTCATAAAATCAATAACGCTTGTACGACTCCGCCCCAATGCttt                                              >  1:507485/1‑150 (MQ=255)
                                                                                                     ccGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTcc                                            <  1:558103/150‑1 (MQ=255)
                                                                                                      cGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                           >  2:765474/1‑150 (MQ=255)
                                                                                                      cGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCa                                           <  1:1585087/150‑1 (MQ=255)
                                                                                                            aaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGt                                     <  1:1070775/150‑1 (MQ=255)
                                                                                                            aaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGt                                     <  1:3398787/150‑1 (MQ=255)
                                                                                                            aaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGt                                     <  1:1790148/150‑1 (MQ=255)
                                                                                                              aGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa                                   >  1:1016605/1‑150 (MQ=255)
                                                                                                               gAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt                                  <  1:2831448/150‑1 (MQ=255)
                                                                                                                 aTCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                                <  2:1175169/150‑1 (MQ=255)
                                                                                                                 aTCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                                <  1:210804/150‑1 (MQ=255)
                                                                                                                  tctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCa                               >  2:3070806/1‑150 (MQ=255)
                                                                                                                  tctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCa                               >  2:3070998/1‑150 (MQ=255)
                                                                                                                   ctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCAt                              <  2:3292270/150‑1 (MQ=255)
                                                                                                                    tcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATg                             >  2:3549388/1‑150 (MQ=255)
                                                                                                                      cACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGcc                           <  1:3677054/150‑1 (MQ=255)
                                                                                                                       aCGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGcca                          <  1:1765525/150‑2 (MQ=255)
                                                                                                                        cGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTg                         <  1:2980843/150‑1 (MQ=255)
                                                                                                                          atatCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                       >  2:637464/1‑150 (MQ=255)
                                                                                                                            atCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAtt                     <  1:451403/150‑1 (MQ=255)
                                                                                                                               gAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGt                  >  2:232908/1‑150 (MQ=255)
                                                                                                                                 gCTGGAAGGCCACCATTTTATTCGCTCGTCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGttt                <  2:3752113/150‑1 (MQ=255)
                                                                                                                                 gCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGttt                <  1:3505420/150‑1 (MQ=255)
                                                                                                                                  cTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc               <  2:1731901/150‑1 (MQ=255)
                                                                                                                                    ggAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACACTCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa             >  2:1766033/1‑150 (MQ=255)
                                                                                                                                    ggAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa             >  2:1764849/1‑150 (MQ=255)
                                                                                                                                    ggAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa             >  2:1513048/1‑150 (MQ=255)
                                                                                                                                     gAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAg            <  1:3331733/150‑1 (MQ=255)
                                                                                                                                     gAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAg            >  1:1326375/1‑150 (MQ=255)
                                                                                                                                      aaGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAgg           >  2:1378564/1‑150 (MQ=255)
                                                                                                                                      aaGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAgg           <  1:1647455/150‑1 (MQ=255)
                                                                                                                                      aaGGCCACCATTTAATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAgg           >  2:2026723/1‑150 (MQ=255)
                                                                                                                                          ccaccaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATcc       >  1:1123337/1‑150 (MQ=255)
                                                                                                                                            accaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCAt     <  1:1728031/150‑1 (MQ=255)
                                                                                                                                               aTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGAc  <  1:615254/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGAC  >  CP009273/3122209‑3122501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑