Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 250,909 A→T L432Q (CTG→CAG)  pepD ← aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273250,9090AT99.6% 833.0 / NA 250L432Q (CTG→CAG) pepDaminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (124/125);  total (125/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

TCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTA  >  CP009273/250762‑251055
                                                                                                                                                   |                                                                                                                                                  
    tcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCcg                                                                                                                                              <  2:3083523/150‑1 (MQ=255)
    tcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCcg                                                                                                                                              >  1:2630712/1‑150 (MQ=255)
    tcAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCcg                                                                                                                                              <  2:568271/150‑1 (MQ=255)
     cagcagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCcgc                                                                                                                                             >  1:3720844/1‑150 (MQ=255)
        cagTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTg                                                                                                                                          <  2:3143509/150‑1 (MQ=255)
         agTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTgg                                                                                                                                         >  1:1843120/1‑150 (MQ=255)
           ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGAt                                                                                                                                       <  1:1592744/150‑1 (MQ=255)
            tcagtcagCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATa                                                                                                                                      <  1:450592/150‑1 (MQ=255)
                   gcagTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGa                                                                                                                               >  1:849121/1‑150 (MQ=255)
                     agTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATg                                                                                                                             <  2:1994837/150‑1 (MQ=255)
                      gtgtCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGt                                                                                                                            <  1:1168176/150‑1 (MQ=255)
                       tgtACAGTAATGACCTACGCTTTCGATGTTAACTTGCTCATTCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCAATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGtt                                                                                                                           <  1:2868588/150‑1 (MQ=255)
                        gtCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTc                                                                                                                          <  2:2746940/150‑1 (MQ=255)
                           cAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGc                                                                                                                       <  2:75981/150‑1 (MQ=255)
                            aGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCg                                                                                                                      >  2:3394601/1‑150 (MQ=255)
                              tAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                                                    <  2:703668/150‑1 (MQ=255)
                              tAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                                                    <  2:869405/150‑1 (MQ=255)
                              tAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                                                    >  1:3615414/1‑150 (MQ=255)
                              tAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                                                    <  2:2609394/150‑1 (MQ=255)
                               aaTGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCt                                                                                                                   <  2:154471/150‑1 (MQ=255)
                                  gACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgt                                                                                                                <  2:2104865/150‑1 (MQ=255)
                                   aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgtt                                                                                                               <  2:1395993/150‑1 (MQ=255)
                                   aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgtt                                                                                                               <  2:959961/150‑1 (MQ=255)
                                    ccTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgttg                                                                                                              <  1:1683053/150‑1 (MQ=255)
                                    ccTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgttg                                                                                                              <  1:724936/150‑1 (MQ=255)
                                    ccTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgttg                                                                                                              <  1:1553152/150‑1 (MQ=255)
                                     cTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                             <  1:2944155/150‑1 (MQ=255)
                                     cTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                             <  1:1027021/150‑1 (MQ=255)
                                     cTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGa                                                                                                             >  1:3287828/1‑150 (MQ=255)
                                      tACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGaa                                                                                                            >  2:2120508/1‑150 (MQ=255)
                                      tACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGaa                                                                                                            >  2:3502816/1‑150 (MQ=255)
                                      tACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCAGGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGAT‑ATCTGGATGTTCGGCGTCTTGTTGAAc                                                                                                           <  2:3124043/150‑1 (MQ=255)
                                         gCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAg                                                                                                         <  2:2675942/150‑1 (MQ=255)
                                          cTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAgg                                                                                                        <  2:2414421/150‑1 (MQ=255)
                                          cTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAgg                                                                                                        >  1:3519931/1‑150 (MQ=255)
                                           tttCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGgc                                                                                                       >  1:2057994/1‑150 (MQ=255)
                                               gATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTg                                                                                                   <  1:3232463/150‑1 (MQ=255)
                                                 tgtgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGAt                                                                                                 >  1:2598095/1‑150 (MQ=255)
                                                   tgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAg                                                                                               >  2:2270291/1‑150 (MQ=255)
                                                   tgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAg                                                                                               <  1:1130653/150‑1 (MQ=255)
                                                     aaCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGt                                                                                             >  1:891095/1‑150 (MQ=255)
                                                      aCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGtt                                                                                            <  1:2430164/150‑1 (MQ=255)
                                                      aCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGtt                                                                                            <  2:409768/150‑1 (MQ=255)
                                                         tGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCa                                                                                         <  1:2581936/150‑1 (MQ=255)
                                                                 cGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAg                                                                                 >  2:2753294/1‑150 (MQ=255)
                                                                  ggAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGa                                                                                <  1:266845/150‑1 (MQ=255)
                                                                   gagaGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGAt                                                                               <  1:612338/150‑1 (MQ=255)
                                                                   gagaGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTAGAACATACCAAATTCCTGGCCCGCGAGGATAATCTGGATTTACGGCGTCTTGTAGAACAGGCGCTGATAGGTTTCACGAACCAGAt                                                                               >  2:1109622/1‑150 (MQ=255)
                                                                     gagTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGc                                                                             <  1:272991/150‑1 (MQ=255)
                                                                       gtgtGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAt                                                                           >  2:1490681/1‑150 (MQ=255)
                                                                             aCCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCgg                                                                     <  2:832350/150‑1 (MQ=255)
                                                                             aCCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCgg                                                                     >  2:924632/1‑150 (MQ=255)
                                                                              ccGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGga                                                                    >  2:547159/1‑150 (MQ=255)
                                                                               cGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGgag                                                                   <  2:2423241/150‑1 (MQ=255)
                                                                                ggTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGgaga                                                                  <  1:334938/150‑1 (MQ=255)
                                                                                ggTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGgaga                                                                  <  1:3501322/150‑1 (MQ=255)
                                                                                  tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAt                                                                >  2:329666/1‑150 (MQ=255)
                                                                                  tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAt                                                                >  2:2292253/1‑150 (MQ=255)
                                                                                   gATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACAGTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAtt                                                               <  2:1657794/150‑1 (MQ=255)
                                                                                    aTAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTa                                                              >  1:1992757/1‑150 (MQ=255)
                                                                                    aTAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTa                                                              >  2:3332810/1‑150 (MQ=255)
                                                                                     tAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAg                                                             >  2:92946/1‑150 (MQ=255)
                                                                                      aGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGc                                                            <  1:125223/150‑1 (MQ=255)
                                                                                       gTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCg                                                           >  1:3764127/1‑150 (MQ=255)
                                                                                       gTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCg                                                           <  1:3403116/150‑1 (MQ=255)
                                                                                        ttGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGACTTAGCGt                                                          >  2:159374/1‑150 (MQ=255)
                                                                                          ggCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTcc                                                        <  1:2949807/150‑1 (MQ=255)
                                                                                              cGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCt                                                    <  1:762049/150‑1 (MQ=255)
                                                                                               gATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTg                                                   <  1:567078/150‑1 (MQ=255)
                                                                                                 tAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgcc                                                 >  2:1235985/1‑150 (MQ=255)
                                                                                                      aCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccagcca                                            <  1:303765/150‑1 (MQ=255)
                                                                                                      aCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccagcca                                            >  2:1314304/1‑150 (MQ=255)
                                                                                                      aCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccagcca                                            >  2:1314774/1‑150 (MQ=255)
                                                                                                         aTGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGa                                         <  1:2287205/150‑1 (MQ=255)
                                                                                                          tGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGat                                        <  2:3725745/150‑1 (MQ=255)
                                                                                                           gTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGata                                       >  2:1843648/1‑150 (MQ=255)
                                                                                                            tCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGatat                                      <  1:3304864/150‑1 (MQ=255)
                                                                                                               aTTTCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATgcg                                   >  1:1759789/1‑150 (MQ=255)
                                                                                                                  tCCGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCt                                <  1:1000999/150‑1 (MQ=255)
                                                                                                                   ccGGATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCtt                               <  1:1400314/150‑1 (MQ=255)
                                                                                                                      gATACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCg                            >  2:3352595/1‑150 (MQ=255)
                                                                                                                        tACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCt                          <  1:2270291/150‑1 (MQ=255)
                                                                                                                        tACGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCt                          >  1:658132/1‑150 (MQ=255)
                                                                                                                         aCGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCtt                         >  2:2961301/1‑150 (MQ=255)
                                                                                                                          cGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTc                        >  1:1290751/1‑150 (MQ=255)
                                                                                                                          cGGTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTc                        <  1:3120223/150‑1 (MQ=255)
                                                                                                                           ggTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCg                       <  2:3430130/150‑1 (MQ=255)
                                                                                                                            gTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCgg                      >  2:732358/1‑150 (MQ=255)
                                                                                                                            gTTTTTTGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCgg                      >  2:664853/1‑150 (MQ=255)
                                                                                                                               ttttGAACAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGttt                   <  1:2793043/150‑1 (MQ=255)
                                                                                                                                    aaCAGACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTcgcg              <  2:1067723/150‑1 (MQ=255)
                                                                                                                                        gACCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccag          >  1:1448414/1‑150 (MQ=255)
                                                                                                                                         aCCACATTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccagc         >  2:981787/1‑150 (MQ=255)
                                                                                                                                            acaTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGccagccag      <  2:3098879/150‑1 (MQ=255)
                                                                                                                                             caTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGt     >  2:2551383/1‑150 (MQ=255)
                                                                                                                                              aTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGtt    >  1:1864405/1‑150 (MQ=255)
                                                                                                                                              aTTCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGtt    >  2:1576051/1‑150 (MQ=255)
                                                                                                                                               ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGGTGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGttt   <  2:2598095/150‑1 (MQ=255)
                                                                                                                                               ttCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGttt   <  2:2811343/150‑1 (MQ=255)
                                                                                                                                                tCCTGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTa  <  1:2405566/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTA  >  CP009273/250762‑251055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑