Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,217,289 A→T H433L (CAC→CTC)  ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,217,2890AT98.9% 594.6 / NA 178H433L (CAC→CTC) ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (99/77);  total (101/77)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

ACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAG  >  CP009273/3217142‑3217435
                                                                                                                                                   |                                                                                                                                                  
aCGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTcg                                                                                                                                                  >  1:2119852/1‑150 (MQ=255)
     cTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAg                                                                                                                                             >  2:1585310/1‑150 (MQ=255)
       gtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaa                                                                                                                                           >  2:1392249/1‑150 (MQ=255)
       gtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaa                                                                                                                                           <  2:408304/150‑1 (MQ=255)
       gtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaa                                                                                                                                           >  2:3784041/1‑150 (MQ=255)
            atatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAcc                                                                                                                                      <  2:1942707/150‑1 (MQ=255)
             tatCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCa                                                                                                                                     >  2:3427385/1‑150 (MQ=255)
                   cGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTc                                                                                                                               >  1:1628447/1‑150 (MQ=255)
                      ccTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGat                                                                                                                            >  2:3504277/1‑150 (MQ=255)
                       cTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatc                                                                                                                           >  1:1955955/1‑150 (MQ=255)
                         gTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcat                                                                                                                         <  1:3537444/150‑1 (MQ=255)
                         gTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcat                                                                                                                         <  1:1717027/150‑1 (MQ=255)
                             gtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcatc                                                                                                                     >  2:117057/1‑150 (MQ=255)
                                    cGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGc                                                                                                              >  2:3375343/1‑150 (MQ=255)
                                     ggAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCt                                                                                                             >  1:2545800/1‑150 (MQ=255)
                                       aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTgg                                                                                                           >  1:3004680/1‑150 (MQ=255)
                                              cGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGa                                                                                                    >  2:2374403/1‑150 (MQ=255)
                                               gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCACTGaa                                                                                                   >  2:1589462/1‑150 (MQ=255)
                                               gTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGaa                                                                                                   >  1:3577068/1‑150 (MQ=255)
                                                tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAAt                                                                                                  <  1:541240/150‑1 (MQ=255)
                                                tCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAAt                                                                                                  <  1:303061/150‑1 (MQ=255)
                                                  gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATcc                                                                                                <  1:662043/150‑1 (MQ=255)
                                                  gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATcc                                                                                                >  2:3015286/1‑150 (MQ=255)
                                                   aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCg                                                                                               >  1:3735306/1‑150 (MQ=255)
                                                   aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCg                                                                                               >  1:3734986/1‑150 (MQ=255)
                                                     tCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGc                                                                                             >  2:1446356/1‑150 (MQ=255)
                                                      cGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                                                                            <  1:1592781/150‑1 (MQ=255)
                                                      cGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                                                                            <  1:3230617/150‑1 (MQ=255)
                                                       gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTa                                                                                           >  2:1385433/1‑150 (MQ=255)
                                                       gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTa                                                                                           <  2:30278/150‑1 (MQ=255)
                                                           ggCTTTGCTAATGTCGGCGATATTCGCCGTATAACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGAATTGATCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCAACATCTGGACGCTGGGCAATGAATCCGGCTATGGc                                                                                       >  2:1720511/1‑150 (MQ=255)
                                                           ggCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGc                                                                                       <  2:359215/150‑1 (MQ=255)
                                                              tttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGt                                                                                    >  2:2169522/1‑150 (MQ=255)
                                                              tttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGt                                                                                    <  1:453821/150‑1 (MQ=255)
                                                              tttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGt                                                                                    >  2:2655703/1‑150 (MQ=255)
                                                               ttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTa                                                                                   <  2:2401893/150‑1 (MQ=255)
                                                               ttGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTAGGGCTGTa                                                                                   <  2:2230057/150‑1 (MQ=255)
                                                                tGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTaa                                                                                  <  2:680676/150‑1 (MQ=255)
                                                                 gCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAAc                                                                                 <  1:924264/150‑1 (MQ=255)
                                                                    aaTGTCGGCGATATGAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATc                                                                              <  1:2284342/150‑1 (MQ=255)
                                                                      tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcg                                                                            <  2:695886/150‑1 (MQ=255)
                                                                            cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGAt                                                                      >  1:2657066/1‑150 (MQ=255)
                                                                            cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGAt                                                                      <  2:3670225/150‑1 (MQ=255)
                                                                             gATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATg                                                                     >  2:3288996/1‑150 (MQ=255)
                                                                             gATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATg                                                                     <  1:264243/150‑1 (MQ=255)
                                                                               tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTa                                                                   <  1:1282087/150‑1 (MQ=255)
                                                                               tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTa                                                                   >  1:455650/1‑150 (MQ=255)
                                                                               tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTa                                                                   >  1:455606/1‑150 (MQ=255)
                                                                               tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTa                                                                   >  1:1401764/1‑150 (MQ=255)
                                                                                atTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAc                                                                  >  1:1007943/1‑150 (MQ=255)
                                                                                 ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAcc                                                                 >  2:2923657/1‑150 (MQ=255)
                                                                                 ttAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAcc                                                                 >  2:1760236/1‑150 (MQ=255)
                                                                                   aGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCAt                                                               >  1:2283651/1‑150 (MQ=255)
                                                                                     ccGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgc                                                             >  1:400196/1‑150 (MQ=255)
                                                                                       gTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcgg                                                           <  2:2725930/150‑1 (MQ=255)
                                                                                       gTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcgg                                                           >  2:2561737/1‑150 (MQ=255)
                                                                                         aTTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcggcg                                                         >  1:1503200/1‑150 (MQ=255)
                                                                                         aTTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcggcg                                                         >  2:1845543/1‑150 (MQ=255)
                                                                                           tACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaa                                                       <  2:3479375/150‑1 (MQ=255)
                                                                                                 cgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCt                                                 <  2:1286635/150‑1 (MQ=255)
                                                                                                 cgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCt                                                 >  1:2439908/1‑150 (MQ=255)
                                                                                                  gaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTg                                                >  2:1669106/1‑150 (MQ=255)
                                                                                                   aTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTgg                                               <  1:1392249/150‑1 (MQ=255)
                                                                                                   aTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTgg                                               >  2:2770701/1‑150 (MQ=255)
                                                                                                    tCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCTCTGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGTCAATGTATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAATCGCTGGa                                              <  2:805361/150‑1 (MQ=255)
                                                                                                      cGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATg                                            >  1:1057149/1‑150 (MQ=255)
                                                                                                       gCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGa                                           >  2:1826176/1‑150 (MQ=255)
                                                                                                         aGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGaca                                         <  1:1776334/150‑1 (MQ=255)
                                                                                                           tGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAcg                                       <  2:141474/150‑1 (MQ=255)
                                                                                                             ggAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAcgcg                                     <  1:3472917/150‑1 (MQ=255)
                                                                                                             ggAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAcgcg                                     <  1:3064421/150‑1 (MQ=255)
                                                                                                              gAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGa                                    >  2:3504265/1‑150 (MQ=255)
                                                                                                                 aaaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTg                                 >  2:854625/1‑150 (MQ=255)
                                                                                                                    gTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                              <  2:2110206/150‑1 (MQ=255)
                                                                                                                    gTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                              <  2:3731324/150‑1 (MQ=255)
                                                                                                                    gTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTg                              <  2:3022743/150‑1 (MQ=255)
                                                                                                                        aCGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCAtt                          >  1:1188339/1‑150 (MQ=255)
                                                                                                                        aCGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCAtt                          <  1:2667464/150‑1 (MQ=255)
                                                                                                                          gTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGACAGCGCTGGATGACACGCGACTGGTGCATTAc                        >  2:2321374/1‑150 (MQ=255)
                                                                                                                          gTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTAc                        >  2:3470935/1‑150 (MQ=255)
                                                                                                                          gTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTAc                        >  2:3475113/1‑150 (MQ=255)
                                                                                                                            cGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACga                      <  1:85106/150‑1 (MQ=255)
                                                                                                                            cGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACga                      <  1:2085597/150‑1 (MQ=255)
                                                                                                                             gAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaa                     >  2:876975/1‑150 (MQ=255)
                                                                                                                              aGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACgaag                    >  2:2566410/1‑150 (MQ=255)
                                                                                                                                 gcATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGaagaag                 >  1:3744733/1‑150 (MQ=255)
                                                                                                                                   aTTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAt               >  1:3152077/1‑150 (MQ=255)
                                                                                                                                   aTTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAt               <  1:1585285/150‑1 (MQ=255)
                                                                                                                                      gTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgc            <  1:2283914/150‑1 (MQ=255)
                                                                                                                                       ttCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg           <  1:2968825/150‑1 (MQ=255)
                                                                                                                                         cGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGAt         <  2:2501491/150‑1 (MQ=255)
                                                                                                                                         cGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGAt         <  2:3577068/150‑1 (MQ=255)
                                                                                                                                          gCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATg        <  1:2084370/150‑1 (MQ=255)
                                                                                                                                           ccATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAACGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGc       >  2:3752541/1‑150 (MQ=255)
                                                                                                                                           ccATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGc       >  1:3440482/1‑150 (MQ=255)
                                                                                                                                             atatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTg     <  2:1554781/150‑1 (MQ=255)
                                                                                                                                             atatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTg     <  1:2210508/150‑1 (MQ=255)
                                                                                                                                              tatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGa    <  2:2826297/150‑1 (MQ=255)
                                                                                                                                               atCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGaa   <  1:619771/150‑1 (MQ=255)
                                                                                                                                               atCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGaa   >  2:3094687/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAG  >  CP009273/3217142‑3217435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑