Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 255,487 A→T S109T (TCT→ACT)  phoE ← outer membrane phosphoporin protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273255,4870AT100.0% 633.8 / NA 194S109T (TCT→ACT) phoEouter membrane phosphoporin protein E
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (83/111);  total (83/111)

Only 100 of 194 total aligned reads displayed.

CGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAAC  >  CP009273/255340‑255633
                                                                                                                                                   |                                                                                                                                                  
 ggTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAcc                                                                                                                                                 <  2:2593116/150‑1 (MQ=255)
  gtgtTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTAccc                                                                                                                                                <  2:2669531/150‑1 (MQ=255)
     ttCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCaaa                                                                                                                                             >  2:2790299/1‑150 (MQ=255)
      tCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAAt                                                                                                                                            <  1:3584653/150‑1 (MQ=255)
       ccGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATc                                                                                                                                           <  2:882034/150‑1 (MQ=255)
       ccGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATc                                                                                                                                           <  2:1219370/150‑1 (MQ=255)
         gATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCtt                                                                                                                                         <  1:3043646/150‑1 (MQ=255)
             cGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTtata                                                                                                                                     <  2:2001143/150‑1 (MQ=255)
             cGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTtata                                                                                                                                     <  2:2218773/150‑1 (MQ=255)
              gTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTatat                                                                                                                                    <  1:2434288/150‑1 (MQ=255)
              gTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTatat                                                                                                                                    <  1:3668549/150‑1 (MQ=255)
              gTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTatat                                                                                                                                    <  1:2799145/150‑1 (MQ=255)
              gTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTatat                                                                                                                                    <  1:2434441/150‑1 (MQ=255)
                cGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATAttt                                                                                                                                  <  2:43781/150‑1 (MQ=255)
                 gCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTc                                                                                                                                 >  1:1775094/1‑150 (MQ=255)
                 gCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTc                                                                                                                                 <  2:2682473/150‑1 (MQ=255)
                   cAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCaa                                                                                                                               >  2:1026645/1‑150 (MQ=255)
                    aGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAc                                                                                                                              <  2:684649/150‑1 (MQ=255)
                    aGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAc                                                                                                                              >  1:400283/1‑150 (MQ=255)
                     gACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAAcc                                                                                                                             <  2:2275097/150‑1 (MQ=255)
                        cGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCgg                                                                                                                          >  1:962373/1‑150 (MQ=255)
                         gCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGc                                                                                                                         <  2:2131729/150‑1 (MQ=255)
                          cTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCa                                                                                                                        <  2:648386/150‑1 (MQ=255)
                          cTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCa                                                                                                                        <  1:3377276/150‑1 (MQ=255)
                           tGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCaa                                                                                                                       >  2:2354852/1‑150 (MQ=255)
                            ggCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCaaa                                                                                                                      >  2:601105/1‑150 (MQ=255)
                              cgcgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAg                                                                                                                    >  1:407736/1‑150 (MQ=255)
                               gcgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGc                                                                                                                   <  2:1072855/150‑1 (MQ=255)
                               gcgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGACACCAAATTCCTGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGc                                                                                                                   <  2:3717803/150‑1 (MQ=255)
                                cgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGTATCGCCACCAACTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATAGAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCg                                                                                                                  <  2:476096/150‑1 (MQ=255)
                                cgTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCg                                                                                                                  <  2:916982/150‑1 (MQ=255)
                                 gTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCga                                                                                                                 >  2:706830/1‑150 (MQ=255)
                                 gTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCga                                                                                                                 >  1:1469767/1‑150 (MQ=255)
                                  tttGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCgag                                                                                                                <  1:53722/150‑1 (MQ=255)
                                    tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAc                                                                                                              <  1:3032693/150‑1 (MQ=255)
                                    tGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGAc                                                                                                              <  1:2668105/150‑1 (MQ=255)
                                         aTAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATACTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtt                                                                                                         >  2:274535/1‑150 (MQ=255)
                                          tAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGACCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTCTATTTCAACCCGGCAAAAGCGAGACGCGttt                                                                                                        >  2:3330599/1‑150 (MQ=255)
                                            aaGTTGTCGGACAGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCTGTCCAGGCTTCAACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAATTAACCAAAACTTTATATTTCAACCCGGAAAAAGAGAGACGAGttttt                                                                                                      >  2:1778822/1‑150 (MQ=255)
                                             aGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGtttttt                                                                                                     <  1:2533654/150‑1 (MQ=255)
                                                tGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCt                                                                                                  >  1:210629/1‑150 (MQ=255)
                                                tGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCt                                                                                                  >  2:1033911/1‑150 (MQ=255)
                                                      tCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAg                                                                                            >  1:3512222/1‑150 (MQ=255)
                                                          cgcCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATc                                                                                        <  1:1094592/150‑1 (MQ=255)
                                                          cgcCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATc                                                                                        >  2:2182605/1‑150 (MQ=255)
                                                           gcCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCa                                                                                       >  2:1612061/1‑150 (MQ=255)
                                                              gAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActc                                                                                    >  2:3753873/1‑150 (MQ=255)
                                                               aGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCActct                                                                                   >  2:496023/1‑150 (MQ=255)
                                                                  aaTCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTg                                                                                >  1:2677370/1‑150 (MQ=255)
                                                                   aTCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGc                                                                               >  1:840200/1‑150 (MQ=255)
                                                                      gCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCttt                                                                            <  1:629295/150‑1 (MQ=255)
                                                                      gCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCttt                                                                            >  2:343321/1‑150 (MQ=255)
                                                                         accaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttatt                                                                         <  1:2319044/150‑1 (MQ=255)
                                                                         accaAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTttatt                                                                         <  2:1517058/150‑1 (MQ=255)
                                                                            aaaTTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTAcc                                                                      >  1:274546/1‑150 (MQ=255)
                                                                              agtCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCgg                                                                    <  2:3430065/148‑1 (MQ=255)
                                                                               ttCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGc                                                                   <  2:775003/150‑1 (MQ=255)
                                                                                  cGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCaaa                                                                >  1:137235/1‑150 (MQ=255)
                                                                                   gggAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAAc                                                               >  2:2323000/1‑150 (MQ=255)
                                                                                     gAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAActc                                                             <  2:1376965/150‑1 (MQ=255)
                                                                                      aaCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAActct                                                            <  1:3022533/150‑1 (MQ=255)
                                                                                      aaCATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAActct                                                            >  2:1263605/1‑150 (MQ=255)
                                                                                          tatCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCtt                                                        <  1:2807456/150‑1 (MQ=255)
                                                                                           atCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTc                                                       >  1:1764199/1‑150 (MQ=255)
                                                                                             cGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTccc                                                     <  2:400283/150‑1 (MQ=255)
                                                                                                 ccAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACg                                                 <  1:2259008/150‑1 (MQ=255)
                                                                                                  cAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGa                                                >  2:2527760/1‑150 (MQ=255)
                                                                                                   aGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGAc                                               >  2:2811345/1‑150 (MQ=255)
                                                                                                   aGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGAc                                               >  2:1672595/1‑150 (MQ=255)
                                                                                                   aGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGAc                                               >  1:710851/1‑150 (MQ=255)
                                                                                                    ggCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGAcc                                              <  1:3272475/150‑1 (MQ=255)
                                                                                                     gCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCa                                             <  1:134138/150‑1 (MQ=255)
                                                                                                      cTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCAt                                            >  1:379671/1‑150 (MQ=255)
                                                                                                      cTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCAt                                            >  1:785941/1‑150 (MQ=255)
                                                                                                          cACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAAcc                                        <  2:2425708/150‑1 (MQ=255)
                                                                                                          cACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAAcc                                        >  1:100273/1‑150 (MQ=255)
                                                                                                           aCGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACca                                       <  1:2411792/150‑1 (MQ=255)
                                                                                                                aTACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtcag                                  >  1:687937/1‑150 (MQ=255)
                                                                                                                 tACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtcagt                                 >  2:741847/1‑150 (MQ=255)
                                                                                                                 tACAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACcagtcagt                                 >  2:951922/1‑150 (MQ=255)
                                                                                                                  aCAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGtt                                <  2:2840152/150‑1 (MQ=255)
                                                                                                                   cAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTg                               <  2:2589551/150‑1 (MQ=255)
                                                                                                                   cAACGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTg                               >  1:277078/1‑150 (MQ=255)
                                                                                                                    aaCGCCCCCAGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGa                              >  2:270912/1‑150 (MQ=255)
                                                                                                                             aGGTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAAtt                     <  2:561521/150‑1 (MQ=255)
                                                                                                                               gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTtg                   <  2:2116162/150‑1 (MQ=255)
                                                                                                                               gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTtg                   <  2:1309165/150‑1 (MQ=255)
                                                                                                                               gTTACGACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTtg                   <  2:1309125/150‑1 (MQ=255)
                                                                                                                                    gACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt              >  2:2537507/1‑150 (MQ=255)
                                                                                                                                    gACCATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGttt              >  2:632396/1‑150 (MQ=255)
                                                                                                                                       cATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGc           >  1:1299473/1‑150 (MQ=255)
                                                                                                                                       cATAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGc           >  2:2095334/1‑150 (MQ=255)
                                                                                                                                        aTAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGcc          >  1:3802147/1‑150 (MQ=255)
                                                                                                                                        aTAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGcc          <  1:2855723/150‑1 (MQ=255)
                                                                                                                                         tAATCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCt         <  1:2057399/150‑1 (MQ=255)
                                                                                                                                          aaTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCtt        <  2:291556/150‑1 (MQ=255)
                                                                                                                                           aTCGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCttt       <  2:3114649/150‑1 (MQ=255)
                                                                                                                                             cGAAAGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGa     <  1:1563483/150‑1 (MQ=255)
                                                                                                                                               aaaGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGaaa   >  1:1131921/1‑150 (MQ=255)
                                                                                                                                                aaGTACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAAc  <  1:1612061/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGTGTTCCGATACGTCGCCAGACCGCTGGCGCGTTTGGTCATAAAGTTGTCGGTCTGCGCCGAGGAATCGCCACCAAATTCCGGGAACATATCGGTCCAGGCTTCCACGTCATACAACGCCCCCAGGTTACGACCATAATCGAAAGAACCCAAATCTTTATATTTCAACCCGGCAAAAGCGAGACGCGTTTTTTGCTGTGCAGTATCACTCTCTGCTTTATTACCGGCAAACTCTGCTTCCCAACGACCATAACCAGTCAGTTGATCGTTAATTTGTGTTTCGCCTTTGAAAC  >  CP009273/255340‑255633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑