Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,302,027 G→T S86* (TCG→TAG)  nlpI ← lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,302,0270GT100.0% 622.3 / NA 186S86* (TCG→TAG) nlpIlipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (101/85);  total (101/85)

Only 100 of 186 total aligned reads displayed.

TTCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGT  >  CP009273/3301880‑3302170
                                                                                                                                                   |                                                                                                                                               
ttCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaa                                                                                                                                               >  2:3724793/1‑150 (MQ=255)
 tCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaa                                                                                                                                              >  2:334909/1‑150 (MQ=255)
 tCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaa                                                                                                                                              >  2:334925/1‑150 (MQ=255)
     gTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATc                                                                                                                                          >  1:601700/1‑150 (MQ=255)
          cgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAc                                                                                                                                     >  2:761403/1‑150 (MQ=255)
          cgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAc                                                                                                                                     <  2:695305/150‑1 (MQ=255)
             aGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAcgcg                                                                                                                                  <  2:1967963/150‑1 (MQ=255)
              gTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACgcgc                                                                                                                                 <  2:1270251/150‑1 (MQ=255)
              gTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACgcgc                                                                                                                                 <  2:313549/150‑1 (MQ=255)
              gTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACgcgc                                                                                                                                 >  2:2727870/1‑150 (MQ=255)
               ttGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCt                                                                                                                                <  1:1867186/150‑1 (MQ=255)
                tGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTa                                                                                                                               >  2:2733107/1‑150 (MQ=255)
                  tAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAAt                                                                                                                             <  2:3800517/150‑1 (MQ=255)
                  tAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAAt                                                                                                                             <  2:282413/150‑1 (MQ=255)
                    aGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGc                                                                                                                           >  1:871529/1‑150 (MQ=255)
                           tCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGa                                                                                                                    <  1:1332411/150‑1 (MQ=255)
                            cAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGAc                                                                                                                   >  1:2083659/1‑150 (MQ=255)
                            cAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGAc                                                                                                                   >  2:3405428/1‑150 (MQ=255)
                               gCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCga                                                                                                                <  1:1946411/150‑1 (MQ=255)
                                cTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCgag                                                                                                               <  1:245537/150‑1 (MQ=255)
                                  cAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGAc                                                                                                             >  2:1916548/1‑150 (MQ=255)
                                  cAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGAc                                                                                                             >  1:2539714/1‑150 (MQ=255)
                                        cAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCAt                                                                                                       <  2:2022267/150‑1 (MQ=255)
                                        cAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCAt                                                                                                       <  2:906933/150‑1 (MQ=255)
                                         aGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATa                                                                                                      >  2:3555830/1‑150 (MQ=255)
                                         aGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATa                                                                                                      <  2:40546/150‑1 (MQ=255)
                                           aaTCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATaca                                                                                                    <  2:2666664/150‑1 (MQ=255)
                                              cAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATacaaca                                                                                                 >  1:2760958/1‑150 (MQ=255)
                                              cAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATacaaca                                                                                                 >  2:2541980/1‑150 (MQ=255)
                                               aaaCGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAacac                                                                                                >  1:488088/1‑150 (MQ=255)
                                                aaCGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACt                                                                                               >  2:3626887/1‑150 (MQ=255)
                                                aaCGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACt                                                                                               <  2:627831/150‑1 (MQ=255)
                                                 aCGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTc                                                                                              <  1:710070/150‑1 (MQ=255)
                                                  cGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTcc                                                                                             <  1:3199624/150‑1 (MQ=255)
                                                  cGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTcc                                                                                             <  1:3472036/150‑1 (MQ=255)
                                                    cTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCcgc                                                                                           >  1:1647370/1‑150 (MQ=255)
                                                    cTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCcgc                                                                                           >  2:612909/1‑150 (MQ=255)
                                                     ttCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCcgcg                                                                                          >  2:674477/1‑150 (MQ=255)
                                                     ttCATAGGCAGCATCAAAATAGCCTGCCTGCGTTAAATATATGCCTAAGTAATGGAATAGTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTAACATACACCACGCcgcg                                                                                          >  2:1682793/1‑150 (MQ=255)
                                                       cATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCt                                                                                        <  2:861077/150‑1 (MQ=255)
                                                        aTAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTc                                                                                       >  1:415906/1‑150 (MQ=255)
                                                         tAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCa                                                                                      >  2:557085/1‑150 (MQ=255)
                                                            gcagcaTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCatat                                                                                   <  2:1321774/150‑1 (MQ=255)
                                                                caTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaaaa                                                                               >  2:2482525/1‑150 (MQ=255)
                                                                 aTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAg                                                                              >  1:356401/1‑150 (MQ=255)
                                                                  tCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGc                                                                             <  2:1536500/150‑1 (MQ=255)
                                                                   cAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCt                                                                            >  1:2719281/1‑150 (MQ=255)
                                                                   cAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCt                                                                            <  2:329211/150‑1 (MQ=255)
                                                                     aaaTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCtgt                                                                          >  1:3443359/1‑150 (MQ=255)
                                                                      aaTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCtgtg                                                                         <  2:1056311/150‑1 (MQ=255)
                                                                         tgcctgccTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTgcgc                                                                      >  1:3036449/1‑150 (MQ=255)
                                                                         tgcctgccTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTgcgc                                                                      >  1:3282468/1‑150 (MQ=255)
                                                                            ctgcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGtt                                                                   >  2:1061100/1‑150 (MQ=255)
                                                                             tgcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTc                                                                  <  2:3437737/150‑1 (MQ=255)
                                                                             tgcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTc                                                                  <  2:2167503/150‑1 (MQ=255)
                                                                               cctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGt                                                                >  2:2069304/1‑150 (MQ=255)
                                                                                ctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTc                                                               >  1:1065433/1‑150 (MQ=255)
                                                                                ctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTc                                                               >  1:1065137/1‑150 (MQ=255)
                                                                                ctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTc                                                               >  1:3190691/1‑150 (MQ=255)
                                                                                ctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTc                                                               >  1:3621739/1‑150 (MQ=255)
                                                                                  gcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCAt                                                             <  1:1701856/150‑1 (MQ=255)
                                                                                   cGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATc                                                            <  1:2123520/150‑1 (MQ=255)
                                                                                      tAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGt                                                         <  2:1145482/150‑1 (MQ=255)
                                                                                        aaTATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTa                                                       <  2:830209/150‑1 (MQ=255)
                                                                                          tatatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTaaa                                                     >  1:822066/1‑150 (MQ=255)
                                                                                          tatatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTaaa                                                     >  1:2217863/1‑150 (MQ=255)
                                                                                           atatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAg                                                    >  1:636985/1‑150 (MQ=255)
                                                                                           atatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAg                                                    <  2:1364518/150‑1 (MQ=255)
                                                                                             atGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGcc                                                  >  2:1733396/1‑150 (MQ=255)
                                                                                               gCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCg                                                >  1:2051384/1‑150 (MQ=255)
                                                                                                ccTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGa                                               >  2:803024/1‑150 (MQ=255)
                                                                                                     gTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGc                                          <  1:669186/150‑1 (MQ=255)
                                                                                                       aaTTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCaa                                        >  2:2947505/1‑150 (MQ=255)
                                                                                                          tGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGa                                     >  1:2992558/1‑150 (MQ=255)
                                                                                                             aTACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAttt                                  <  2:1054306/150‑1 (MQ=255)
                                                                                                                 ttCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTc                              <  2:1084014/150‑1 (MQ=255)
                                                                                                                  tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTcc                             >  1:2295253/1‑150 (MQ=255)
                                                                                                                  tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTcc                             <  1:2492996/150‑1 (MQ=255)
                                                                                                                  tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTcc                             <  1:3405428/150‑1 (MQ=255)
                                                                                                                   cAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCa                            >  1:474475/1‑150 (MQ=255)
                                                                                                                    aGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCAt                           <  1:1217305/150‑1 (MQ=255)
                                                                                                                       cATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACg                        >  2:1856059/1‑150 (MQ=255)
                                                                                                                          atCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGc                     >  2:1499308/1‑150 (MQ=255)
                                                                                                                          atCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGc                     >  1:1509176/1‑150 (MQ=255)
                                                                                                                            ccGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAGAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCa                   >  2:701073/1‑150 (MQ=255)
                                                                                                                            ccGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCa                   >  2:701072/1‑150 (MQ=255)
                                                                                                                            ccGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCa                   >  2:701402/1‑150 (MQ=255)
                                                                                                                               gTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGaa                >  1:339800/1‑150 (MQ=255)
                                                                                                                                  ggATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCa             >  1:1621821/1‑150 (MQ=255)
                                                                                                                                  ggATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCa             <  2:2988979/150‑1 (MQ=255)
                                                                                                                                  ggATTGCCAGCGCTTGCTAAAAATCGTTACGCGATAATGCCCTCAGACCGAGACTATCATACTACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCa             <  1:1849493/150‑1 (MQ=255)
                                                                                                                                   gATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCAc            <  1:1732849/150‑1 (MQ=255)
                                                                                                                                     ttGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACtt          <  1:823773/150‑1 (MQ=255)
                                                                                                                                     ttGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACtt          <  1:2727870/150‑1 (MQ=255)
                                                                                                                                         cAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctg      <  2:2375720/150‑1 (MQ=255)
                                                                                                                                         cAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctg      >  1:2553311/1‑150 (MQ=255)
                                                                                                                                          aGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctgc     <  1:1916591/150‑1 (MQ=255)
                                                                                                                                          aGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctgc     >  1:308583/1‑150 (MQ=255)
                                                                                                                                           gcgcTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctgct    <  1:1419416/150‑1 (MQ=255)
                                                                                                                                            cgcTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctgctg   >  1:3502249/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TTCAAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGT  >  CP009273/3301880‑3302170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑