BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,340,038	C→A	I34I (ATC→ATA)	ptsN →	sugar‑specific enzyme IIA component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,340,038	0	C	A	100.0%	711.5 / NA	214	I34I (ATC→ATA)	ptsN	sugar‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (106/108); total (106/108)

Only 100 of 222 total aligned reads displayed.

CGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCG  >  CP009273/3339892‑3340181

 gAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAAtaatca                                                                                                                                             <  2:457736/150‑1 (MQ=255)

 gAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAAtaatca                                                                                                                                             >  1:1361913/1‑150 (MQ=255)

 gAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAAtaatca                                                                                                                                             <  2:275693/150‑1 (MQ=255)

    ccTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAGATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCg                                                                                                                                          <  1:1050341/150‑1 (MQ=255)

     cTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGa                                                                                                                                         >  2:1697026/1‑150 (MQ=255)

         tCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTg                                                                                                                                     >  2:2190699/1‑150 (MQ=255)

          ccACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTgg                                                                                                                                    <  2:357749/150‑1 (MQ=255)

               gTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGgcggcg                                                                                                                               <  1:2956041/150‑1 (MQ=255)

                tttGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGa                                                                                                                              <  2:1641623/150‑1 (MQ=255)

                 ttGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGaa                                                                                                                             >  1:283879/1‑150 (MQ=255)

                   gAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGTAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAaac                                                                                                                           <  1:2567685/150‑1 (MQ=255)

                   gAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAaac                                                                                                                           <  2:2134545/150‑1 (MQ=255)

                   gAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAaac                                                                                                                           <  1:105862/150‑1 (MQ=255)

                    aGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAaaca                                                                                                                          >  1:288983/1‑150 (MQ=255)

                     gTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAaacaa                                                                                                                         <  1:3652001/150‑1 (MQ=255)

                      tGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAaacaac                                                                                                                        <  2:3549856/150‑1 (MQ=255)

                        ggCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACtt                                                                                                                      >  1:1467713/1‑150 (MQ=255)

                            ggTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGcc                                                                                                                  <  2:3196082/150‑1 (MQ=255)

                            ggTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGcc                                                                                                                  >  1:2478762/1‑150 (MQ=255)

                             gTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCt                                                                                                                 <  1:2463410/150‑1 (MQ=255)

                                cTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTgcc                                                                                                              <  2:1804898/150‑1 (MQ=255)

                                cTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTgcc                                                                                                              >  1:788632/1‑150 (MQ=255)

                                cTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTgcc                                                                                                              >  1:1022975/1‑150 (MQ=255)

                                   aGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTgccgcc                                                                                                           >  2:823013/1‑150 (MQ=255)

                                    ggTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCt                                                                                                          >  2:2142631/1‑150 (MQ=255)

                                      tGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCa                                                                                                        <  1:408386/150‑1 (MQ=255)

                                         aaTTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAggt                                                                                                     >  2:3668634/1‑150 (MQ=255)

                                         aaTTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAggt                                                                                                     <  1:3141373/150‑1 (MQ=255)

                                          aTTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCTGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAggng                                                                                                    <  1:735642/150‑1 (MQ=255)

                                           ttATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAggtgg                                                                                                   <  2:3495899/150‑1 (MQ=255)

                                              tGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGttt                                                                                                >  1:1864098/1‑150 (MQ=255)

                                                aCAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGttttt                                                                                              >  2:3522457/1‑150 (MQ=255)

                                                aCAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGttttt                                                                                              >  2:1237049/1‑150 (MQ=255)

                                                aCAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGttttt                                                                                              <  2:1051403/150‑1 (MQ=255)

                                                 cAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTg                                                                                             <  2:3584282/150‑1 (MQ=255)

                                                   aataatGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGaa                                                                                           >  1:3543044/1‑150 (MQ=255)

                                                      aatGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCt                                                                                        <  2:1308415/150‑1 (MQ=255)

                                                      aatGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCt                                                                                        <  1:1294599/150‑1 (MQ=255)

                                                      aatGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCt                                                                                        <  2:1437352/150‑1 (MQ=255)

                                                         gATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATc                                                                                     >  1:3497432/1‑150 (MQ=255)

                                                             cAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGa                                                                                 >  2:2205924/1‑150 (MQ=255)

                                                              aaCTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGAc                                                                                <  2:3782845/150‑1 (MQ=255)

                                                              aaCTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGAc                                                                                <  1:903198/150‑1 (MQ=255)

                                                              aaCTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGAc                                                                                >  1:1626393/1‑150 (MQ=255)

                                                               aCTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGAcg                                                                               >  1:595214/1‑150 (MQ=255)

                                                                  ctACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCgg                                                                            >  2:284710/1‑150 (MQ=255)

                                                                  ctACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCgg                                                                            >  2:3738784/1‑150 (MQ=255)

                                                                  ctACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCgg                                                                            >  1:27101/1‑150 (MQ=255)

                                                                     cAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGaa                                                                         <  2:2947038/150‑1 (MQ=255)

                                                                          tAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAAt                                                                    >  2:2478264/1‑150 (MQ=255)

                                                                           aGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATg                                                                   <  2:1887048/150‑1 (MQ=255)

                                                                             cAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATggg                                                                 >  2:668534/1‑150 (MQ=255)

                                                                             cAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATggg                                                                 >  1:1274251/1‑150 (MQ=255)

                                                                              aGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGc                                                                <  2:2876864/150‑1 (MQ=255)

                                                                               gtgtTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGTGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCa                                                               >  1:1736111/1‑150 (MQ=255)

                                                                               gtgtTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGTGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCa                                                               >  1:1736042/1‑150 (MQ=255)

                                                                                 gtTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGt                                                             <  1:1346397/150‑1 (MQ=255)

                                                                                 gtTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGt                                                             >  1:3552465/1‑150 (MQ=255)

                                                                                 gtTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGt                                                             <  1:1604140/150‑1 (MQ=255)

                                                                                  ttCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTa                                                            <  1:1511075/150‑1 (MQ=255)

                                                                                    cTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTAcc                                                          >  1:1333180/1‑150 (MQ=255)

                                                                                    cTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTAcc                                                          <  1:1777909/150‑1 (MQ=255)

                                                                                      tAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCgg                                                        >  2:3390884/1‑150 (MQ=255)

                                                                                        aCAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTa                                                      <  1:451512/150‑1 (MQ=255)

                                                                                         cAGGGTATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGAGTCGAAACAACTTAGACTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTAt                                                     <  1:3283310/150‑1 (MQ=255)

                                                                                          aGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATc                                                    <  2:537351/150‑1 (MQ=255)

                                                                                           gggAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCg                                                   >  2:2322438/1‑150 (MQ=255)

                                                                                              aaTGTACGCGAAGCCGCGTCCACTGTAAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAACTGGCGGCGAAACAACTTATCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCa                                                >  1:3298256/1‑150 (MQ=255)

                                                                                                tGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAAt                                              <  2:3032918/150‑1 (MQ=255)

                                                                                                   aCGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGt                                           >  2:3120749/1‑150 (MQ=255)

                                                                                                      cgAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTAtt                                        <  1:2375094/150‑1 (MQ=255)

                                                                                                       gAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTg                                       >  2:337636/1‑150 (MQ=255)

                                                                                                        aaGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATGCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGc                                      >  1:1899794/1‑150 (MQ=255)

                                                                                                            cgcgTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCAtt                                  <  1:1493940/150‑1 (MQ=255)

                                                                                                             gcgTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTc                                 <  2:788632/150‑1 (MQ=255)

                                                                                                             gcgTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTc                                 <  2:1022975/150‑1 (MQ=255)

                                                                                                              cgTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTcc                                >  2:1588362/1‑150 (MQ=255)

                                                                                                              cgTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTcc                                <  2:3436589/150‑1 (MQ=255)

                                                                                                               gTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCg                               >  1:964499/1‑150 (MQ=255)

                                                                                                               gTCCACAGTCAGAGCAAAAACCGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCg                               <  2:259663/150‑1 (MQ=255)

                                                                                                                    cTGTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATgg                          <  2:1991226/150‑1 (MQ=255)

                                                                                                                      gTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCa                        <  2:3207026/150‑1 (MQ=255)

                                                                                                                      gTCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCa                        >  1:2610138/1‑150 (MQ=255)

                                                                                                                       tCAGAGCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCaa                       <  2:27101/150‑1 (MQ=255)

                                                                                                                         agagCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAAc                     <  2:706837/150‑1 (MQ=255)

                                                                                                                         agagCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAAc                     >  1:1240744/1‑150 (MQ=255)

                                                                                                                         agagCAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAAc                     <  2:1361913/150‑1 (MQ=255)

                                                                                                                             cAAAAAACGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGga                 <  2:2353567/150‑1 (MQ=255)

                                                                                                                              aaaaaaCGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGgaa                >  1:2155543/1‑150 (MQ=255)

                                                                                                                               aaaaaCGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGgaag               <  1:2477725/150‑1 (MQ=255)

                                                                                                                                aaaaCGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGgaaga              <  1:3387959/150‑1 (MQ=255)

                                                                                                                                  aaCGCGCCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGaagaag            >  2:3442009/1‑150 (MQ=255)

                                                                                                                                     gcgcCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATa         <  2:1320892/150‑1 (MQ=255)

                                                                                                                                     gcgcCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAAAAGATa         <  1:2426212/150‑1 (MQ=255)

                                                                                                                                       gcCCTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATAct       >  2:437901/1‑150 (MQ=255)

                                                                                                                                         ccTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATActct     <  2:1625718/150‑1 (MQ=255)

                                                                                                                                         ccTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATActct     >  1:3079777/1‑150 (MQ=255)

                                                                                                                                          cTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTg    >  2:2543813/1‑150 (MQ=255)

                                                                                                                                          cTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTg    <  2:410018/150‑1 (MQ=255)

                                                                                                                                          cTGGAAATAATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTg    >  1:223249/1‑150 (MQ=255)

CGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTATCCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCG  >  CP009273/3339892‑3340181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`