Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,351,650 G→T E1189D (GAG→GAT)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,351,6500GT100.0% 846.8 / NA 255E1189D (GAG→GAT) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (111/144);  total (111/144)

Only 100 of 255 total aligned reads displayed.

TGAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCG  >  CP009273/3351503‑3351796
                                                                                                                                                   |                                                                                                                                                  
tGAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATAc                                                                                                                                                  <  2:1142379/150‑1 (MQ=255)
 gAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACt                                                                                                                                                 <  1:1051877/150‑1 (MQ=255)
 gAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACt                                                                                                                                                 <  2:697647/150‑1 (MQ=255)
  aGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTg                                                                                                                                                >  1:597939/1‑150 (MQ=255)
    tttATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGcc                                                                                                                                              >  1:2983703/1‑150 (MQ=255)
     ttATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCg                                                                                                                                             >  1:1115925/1‑150 (MQ=255)
     ttATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCg                                                                                                                                             >  2:2818397/1‑150 (MQ=255)
     ttATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCg                                                                                                                                             >  1:3016825/1‑150 (MQ=255)
        tCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAAc                                                                                                                                          >  2:1971630/1‑150 (MQ=255)
        tCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAAc                                                                                                                                          <  1:784259/150‑1 (MQ=255)
           cccGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGc                                                                                                                                       >  2:1368232/1‑150 (MQ=255)
              gTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATc                                                                                                                                    <  2:3509332/150‑1 (MQ=255)
                 aaaCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAg                                                                                                                                 <  1:962128/150‑1 (MQ=255)
                   aCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGt                                                                                                                               <  1:1738641/150‑1 (MQ=255)
                    cccGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTa                                                                                                                              >  2:151424/1‑150 (MQ=255)
                     ccGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTaa                                                                                                                             >  2:2684302/1‑150 (MQ=255)
                      cgcgAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAg                                                                                                                            <  2:3784742/150‑1 (MQ=255)
                      cgcgAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAg                                                                                                                            >  2:173227/1‑150 (MQ=255)
                          aGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCAc                                                                                                                        >  2:3697154/1‑150 (MQ=255)
                             tGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGATGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCActct                                                                                                                     <  1:1477712/150‑1 (MQ=255)
                             tGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCActct                                                                                                                     <  1:11820/150‑1 (MQ=255)
                                tGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACt                                                                                                                  <  2:330537/150‑1 (MQ=255)
                                tGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACt                                                                                                                  <  2:311544/150‑1 (MQ=255)
                                tGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACt                                                                                                                  >  2:3066439/1‑150 (MQ=255)
                                tGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACt                                                                                                                  <  1:1368232/150‑1 (MQ=255)
                                 ggCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTg                                                                                                                 <  2:2981392/150‑1 (MQ=255)
                                        cTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaa                                                                                                          <  2:1330129/150‑1 (MQ=255)
                                          tGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaaa                                                                                                        <  2:406718/150‑1 (MQ=255)
                                           ggCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaac                                                                                                       <  2:2480432/150‑1 (MQ=255)
                                           ggCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaac                                                                                                       >  2:132803/1‑150 (MQ=255)
                                               tAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAAcc                                                                                                   >  2:812276/1‑150 (MQ=255)
                                                aaCACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAAccc                                                                                                  <  1:2255517/150‑1 (MQ=255)
                                                  cacGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACccgc                                                                                                >  1:1717742/1‑150 (MQ=255)
                                                   acGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACccgcc                                                                                               <  1:3443079/150‑1 (MQ=255)
                                                    cGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACccgccg                                                                                              >  1:3449927/1‑150 (MQ=255)
                                                      tCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCATACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGtt                                                                                            <  2:750327/150‑1 (MQ=255)
                                                      tCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGtt                                                                                            <  2:979382/150‑1 (MQ=255)
                                                      tCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGtt                                                                                            <  1:47689/150‑1 (MQ=255)
                                                      tCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGtt                                                                                            <  1:3230766/150‑1 (MQ=255)
                                                       cTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGttt                                                                                           <  2:208408/150‑1 (MQ=255)
                                                          gtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGAt                                                                                        <  1:3168618/150‑1 (MQ=255)
                                                           tggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATa                                                                                       >  1:119456/1‑150 (MQ=255)
                                                           tggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATa                                                                                       <  2:119456/150‑1 (MQ=255)
                                                              aTCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACg                                                                                    <  1:927850/150‑1 (MQ=255)
                                                                cTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGc                                                                                  <  2:2117784/150‑1 (MQ=255)
                                                                 tGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                                 >  1:71135/1‑150 (MQ=255)
                                                                 tGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                                 >  2:3196947/1‑150 (MQ=255)
                                                                 tGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                                 >  2:150614/1‑150 (MQ=255)
                                                                  gATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCct                                                                                >  1:160162/1‑150 (MQ=255)
                                                                         cGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAc                                                                         <  1:3663133/150‑1 (MQ=255)
                                                                          gCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcg                                                                        <  1:2740468/150‑1 (MQ=255)
                                                                            aCCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg                                                                      <  2:2523951/150‑1 (MQ=255)
                                                                              cGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCa                                                                    >  2:660254/1‑150 (MQ=255)
                                                                               gACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAg                                                                   >  2:1177119/1‑150 (MQ=255)
                                                                                aCCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGt                                                                  >  2:3343899/1‑150 (MQ=255)
                                                                                 ccTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGtt                                                                 <  1:235299/150‑1 (MQ=255)
                                                                                 ccTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGtt                                                                 <  2:2233063/150‑1 (MQ=255)
                                                                                 ccTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGtt                                                                 <  2:325489/150‑1 (MQ=255)
                                                                                 ccTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGtt                                                                 <  2:2313063/150‑1 (MQ=255)
                                                                                  ctgctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtg                                                                <  2:16641/150‑1 (MQ=255)
                                                                                     ctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgctg                                                             <  1:2733549/150‑1 (MQ=255)
                                                                                     ctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGACGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgctg                                                             <  1:2307167/150‑1 (MQ=255)
                                                                                       gAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCa                                                           <  1:1940875/150‑1 (MQ=255)
                                                                                       gAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCa                                                           <  1:3647301/150‑1 (MQ=255)
                                                                                         aaGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAAc                                                         <  2:1656983/150‑1 (MQ=255)
                                                                                         aaGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAAc                                                         >  2:1427144/1‑150 (MQ=255)
                                                                                           gagCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAg                                                       <  1:517177/150‑1 (MQ=255)
                                                                                              cTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCg                                                    <  1:2056818/150‑1 (MQ=255)
                                                                                              cTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCg                                                    <  2:3016825/150‑1 (MQ=255)
                                                                                              cTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCg                                                    <  1:320888/150‑1 (MQ=255)
                                                                                                   cGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAAcc                                               <  1:2607782/150‑1 (MQ=255)
                                                                                                    ggTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCg                                              >  2:162816/1‑150 (MQ=255)
                                                                                                    ggTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCg                                              <  1:2845945/150‑1 (MQ=255)
                                                                                                     gTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt                                             <  1:1084177/150‑1 (MQ=255)
                                                                                                     gTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGt                                             <  2:2468502/150‑1 (MQ=255)
                                                                                                      tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGtt                                            <  1:2496353/150‑1 (MQ=255)
                                                                                                      tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGtt                                            >  1:1650705/1‑150 (MQ=255)
                                                                                                      tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGtt                                            >  1:3546218/1‑150 (MQ=255)
                                                                                                       ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGttt                                           <  2:2597058/150‑1 (MQ=255)
                                                                                                          aCCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGACAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTc                                        >  2:2345923/1‑150 (MQ=255)
                                                                                                             gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGAt                                     >  2:50192/1‑150 (MQ=255)
                                                                                                               cAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGa                                   >  2:1780295/1‑150 (MQ=255)
                                                                                                                aaaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAg                                  >  1:1471169/1‑150 (MQ=255)
                                                                                                                aaaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACAGCATCCAGGTAATGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGACCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAg                                  >  1:2125583/1‑150 (MQ=255)
                                                                                                                 aaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAgc                                 >  2:1882667/1‑150 (MQ=255)
                                                                                                                 aaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAgc                                 >  2:1834118/1‑150 (MQ=255)
                                                                                                                    agcagAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGcc                              <  2:2890365/150‑1 (MQ=255)
                                                                                                                    agcagAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGcc                              >  1:807705/1‑150 (MQ=255)
                                                                                                                      cagAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCag                            <  2:2149913/150‑1 (MQ=255)
                                                                                                                      cagAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCag                            <  1:419414/150‑1 (MQ=255)
                                                                                                                        gAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCagag                          >  1:1881122/1‑150 (MQ=255)
                                                                                                                              ggCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAAc                    <  1:1996546/150‑1 (MQ=255)
                                                                                                                                   tGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCt               <  1:524469/150‑1 (MQ=255)
                                                                                                                                   tGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACACTACTGCACCGAAAACAACCCACCAATTGATAACGACCTGCTGAACTCGCAGTTGCTACAACAAGCGAAACCGATTGTCGATGAGCGCCAGAGCAAAACCTACt               >  1:1131318/1‑150 (MQ=255)
                                                                                                                                    gTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTg              >  1:2361521/1‑150 (MQ=255)
                                                                                                                                     tCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTgg             <  2:3427406/150‑1 (MQ=255)
                                                                                                                                         aGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCg         <  2:454026/150‑1 (MQ=255)
                                                                                                                                          gctgctGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGa        <  1:856949/150‑1 (MQ=255)
                                                                                                                                           ctgctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGat       <  1:348810/150‑1 (MQ=255)
                                                                                                                                               tgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTc   >  1:2473538/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCG  >  CP009273/3351503‑3351796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑