Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,373,985 C→A intergenic (+37/‑117) yhcB → / → degQ DUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,373,9850CA100.0% 749.1 / NA 234intergenic (+37/‑117)yhcB/degQDUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (85/149);  total (85/149)

Only 100 of 241 total aligned reads displayed.

AATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  >  CP009273/3373838‑3374129
                                                                                                                                                   |                                                                                                                                                
aaTCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTtatc                                                                                                                                                >  1:1337717/1‑150 (MQ=255)
 aTCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTtatct                                                                                                                                               >  1:1686946/1‑150 (MQ=255)
     tCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATctccct                                                                                                                                           <  1:2014425/150‑1 (MQ=255)
       tGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATctccctcc                                                                                                                                         <  1:612270/150‑1 (MQ=255)
        ggCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCtccctccc                                                                                                                                        >  1:2824558/1‑150 (MQ=255)
           agagTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGa                                                                                                                                     >  1:3799937/1‑150 (MQ=255)
             agTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACt                                                                                                                                   <  1:3074371/150‑1 (MQ=255)
               tCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTAt                                                                                                                                 <  2:3773927/150‑1 (MQ=255)
                  gAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCAt                                                                                                                              >  1:1427920/1‑150 (MQ=255)
                   aaGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCAtt                                                                                                                             <  1:3685907/150‑1 (MQ=255)
                     gCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATCTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTa                                                                                                                           <  1:1711540/150‑1 (MQ=255)
                      ccAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTaa                                                                                                                          <  2:662746/150‑1 (MQ=255)
                       cAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAAt                                                                                                                         >  1:2364684/1‑150 (MQ=255)
                        aGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATc                                                                                                                        <  1:862227/150‑1 (MQ=255)
                          cAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTg                                                                                                                      >  2:1491858/1‑150 (MQ=255)
                            aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGgt                                                                                                                    <  2:3438667/150‑1 (MQ=255)
                            aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGgt                                                                                                                    >  2:2168035/1‑150 (MQ=255)
                            aCGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCACTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGgt                                                                                                                    >  2:257835/1‑150 (MQ=255)
                               aTCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGtc                                                                                                                 <  2:3153104/150‑1 (MQ=255)
                                   ggCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCAt                                                                                                             <  1:1635661/150‑1 (MQ=255)
                                    gCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCAtt                                                                                                            >  2:400587/1‑150 (MQ=255)
                                     cACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTg                                                                                                           >  1:1753036/1‑150 (MQ=255)
                                     cACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTg                                                                                                           <  2:1599694/150‑1 (MQ=255)
                                      aCCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGt                                                                                                          <  1:1857424/150‑1 (MQ=255)
                                         ggTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTTTTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAg                                                                                                       <  1:244602/150‑1 (MQ=255)
                                           tGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGcc                                                                                                     <  1:541793/150‑1 (MQ=255)
                                           tGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGcc                                                                                                     <  2:1583761/150‑1 (MQ=255)
                                            gCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCg                                                                                                    <  1:3041543/150‑1 (MQ=255)
                                              aGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTc                                                                                                  >  2:1176039/1‑150 (MQ=255)
                                                 tGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                               >  1:3447104/1‑150 (MQ=255)
                                                 tGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                               <  2:1499842/150‑1 (MQ=255)
                                                 tGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGa                                                                                               <  1:106667/150‑1 (MQ=255)
                                                   ccTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGaaa                                                                                             <  1:3649814/150‑1 (MQ=255)
                                                    cTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGaaaa                                                                                            >  1:1156631/1‑150 (MQ=255)
                                                      cgcgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAAtt                                                                                          <  1:2127541/150‑1 (MQ=255)
                                                      cgcgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAAtt                                                                                          <  1:3530345/150‑1 (MQ=255)
                                                       gcgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTc                                                                                         <  2:3243490/150‑1 (MQ=255)
                                                       gcgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTc                                                                                         <  2:3243459/150‑1 (MQ=255)
                                                        cgACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCa                                                                                        >  1:49044/1‑150 (MQ=255)
                                                         gACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       >  2:1946151/1‑150 (MQ=255)
                                                         gACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       >  1:997978/1‑150 (MQ=255)
                                                         gACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       <  1:2416872/150‑1 (MQ=255)
                                                         gACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCaa                                                                                       >  1:803910/1‑150 (MQ=255)
                                                           cTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATa                                                                                     <  2:2051927/150‑1 (MQ=255)
                                                             aTTCTGCAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTACTTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAAc                                                                                   <  2:2835883/150‑1 (MQ=255)
                                                              ttCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACa                                                                                  <  2:2912698/150‑1 (MQ=255)
                                                               tCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACAt                                                                                 <  2:383239/150‑1 (MQ=255)
                                                                  gAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCaa                                                                              <  2:1627873/150‑1 (MQ=255)
                                                                     ggCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTTATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTATCCGTGTGAAAATTCAATAACATCAAACt                                                                           <  1:3571136/150‑1 (MQ=255)
                                                                      gcgcATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTg                                                                          <  2:3382458/150‑1 (MQ=255)
                                                                       cgcATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGt                                                                         <  2:2355936/150‑1 (MQ=255)
                                                                         cATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGttt                                                                       <  1:1485252/150‑1 (MQ=255)
                                                                         cATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGttt                                                                       <  2:1747927/150‑1 (MQ=255)
                                                                          aTCCGGCCTGCTGCGTACTGGCGNGAAGCGNGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTANCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCNTCTGAAAATTCAATAACATCAAACTGtttt                                                                      >  2:2417729/1‑150 (MQ=255)
                                                                          aTCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGtttt                                                                      <  1:3232064/150‑1 (MQ=255)
                                                                             cGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGaa                                                                   >  1:1514249/1‑150 (MQ=255)
                                                                              ggCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAAt                                                                  <  2:3570366/150‑1 (MQ=255)
                                                                                   gctgcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCtt                                                             <  2:574155/150‑1 (MQ=255)
                                                                                     tgcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCtttt                                                           >  2:2397270/1‑150 (MQ=255)
                                                                                      gcGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTc                                                          <  1:3404273/150‑1 (MQ=255)
                                                                                        gTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCtt                                                        <  2:1897570/150‑1 (MQ=255)
                                                                                         tACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTa                                                       >  1:1606780/1‑150 (MQ=255)
                                                                                         tACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTa                                                       <  2:1670303/150‑1 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                      <  1:3001327/150‑1 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                      >  1:2876364/1‑150 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                      <  2:2267725/150‑1 (MQ=255)
                                                                                          aCTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTAt                                                      >  1:974047/1‑150 (MQ=255)
                                                                                             ggCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAt                                                   <  1:1577562/150‑1 (MQ=255)
                                                                                             ggCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAt                                                   <  1:3183803/150‑1 (MQ=255)
                                                                                             ggCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAt                                                   <  2:1222667/150‑1 (MQ=255)
                                                                                              gcgcGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCAtt                                                  <  2:2727396/150‑1 (MQ=255)
                                                                                               cgcgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTc                                                 <  2:1998450/150‑1 (MQ=255)
                                                                                               cgcgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTc                                                 <  2:2659869/150‑1 (MQ=255)
                                                                                               cgcgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTc                                                 >  2:629546/1‑150 (MQ=255)
                                                                                                 cgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAg                                               >  2:3519709/1‑150 (MQ=255)
                                                                                                 cgAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAg                                               >  1:253803/1‑150 (MQ=255)
                                                                                                  gAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAgg                                              <  2:734060/150‑1 (MQ=255)
                                                                                                  gAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAgg                                              >  2:3674550/1‑150 (MQ=255)
                                                                                                   aaGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGt                                             <  2:1337717/150‑1 (MQ=255)
                                                                                                     gcgcGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTAc                                           <  2:375135/150‑1 (MQ=255)
                                                                                                      cgcgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACg                                          <  2:826659/150‑1 (MQ=255)
                                                                                                       gcgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACga                                         <  2:3195862/150‑1 (MQ=255)
                                                                                                        cgACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACgag                                        <  1:1974005/150‑1 (MQ=255)
                                                                                                             aaTTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAg                                   <  2:1737900/150‑1 (MQ=255)
                                                                                                             aaTTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAg                                   >  1:2184365/1‑150 (MQ=255)
                                                                                                              atttatttTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAgg                                  <  1:1056609/150‑1 (MQ=255)
                                                                                                              atttatttTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAgg                                  >  2:611255/1‑150 (MQ=255)
                                                                                                                ttatttTTCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGaa                                >  2:1059529/1‑150 (MQ=255)
                                                                                                                       tCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaa                         <  2:1032401/150‑1 (MQ=255)
                                                                                                                       tCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaa                         <  2:2062585/150‑1 (MQ=255)
                                                                                                                       tCGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaa                         >  1:1197593/1‑150 (MQ=255)
                                                                                                                        cGGGCGCAGCCATTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGaaa                        >  1:2454194/1‑150 (MQ=255)
                                                                                                                               aGCCAATGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTATTATCATTCAGGTACGAGAGCAGGAATAATGAAAaaacaaa                 >  1:1286778/1‑150 (MQ=255)
                                                                                                                                   aTTGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCa             <  2:2336747/150‑1 (MQ=255)
                                                                                                                                     tGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGc           <  1:3504703/150‑1 (MQ=255)
                                                                                                                                     tGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGc           <  1:2287748/150‑1 (MQ=255)
                                                                                                                                     tGCGCCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGc           >  2:2138248/1‑150 (MQ=255)
                                                                                                                                      gcgcCCTCCTCTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCt          <  2:1686946/150‑1 (MQ=255)
                                                                                                                                            tcctcTTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAg    <  1:2948423/150‑1 (MQ=255)
                                                                                                                                              ctctTATCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTg  <  1:3357712/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTTTTCGGGCGCAGCCATTGCGCCCTCCTCTTCTCTCCCTCCCCGACTATCATTTAATCTGGTGTCTCATTGTTAGCCGTCTGAAAATTCAATAACATCAAACTGTTTTGAATCTCTTTTCTTATCATTCAGGTACGAGAGCAGGAATAATGAAAAAACAAACCCAGCTGTTGAGTG  >  CP009273/3373838‑3374129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑