Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 259,494 G→T C75* (TGC→TGA)  yafW ← CP4‑6 prophage; antitoxin of the YkfI‑YafW toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273259,4940GT100.0% 796.6 / NA 236C75* (TGC→TGA) yafWCP4‑6 prophage; antitoxin of the YkfI‑YafW toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (110/126);  total (110/126)

Only 100 of 236 total aligned reads displayed.

CCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATGCAG  >  CP009273/259347‑259640
                                                                                                                                                   |                                                                                                                                                  
  gcccgcTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGt                                                                                                                                                <  1:1748636/150‑1 (MQ=255)
   cccgcTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTg                                                                                                                                               >  2:1593389/1‑150 (MQ=255)
    ccgcTGTGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTgg                                                                                                                                              <  1:885546/150‑1 (MQ=255)
     cgcTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTggg                                                                                                                                             >  1:2963092/1‑150 (MQ=255)
       cTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGGTGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCa                                                                                                                                           >  2:3207921/1‑150 (MQ=255)
        tGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCAt                                                                                                                                          >  2:3438150/1‑150 (MQ=255)
         gAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCAtg                                                                                                                                         <  2:3069743/150‑1 (MQ=255)
         gAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCAtg                                                                                                                                         <  1:3434789/150‑1 (MQ=255)
          aGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCAtgg                                                                                                                                        >  2:3142624/1‑150 (MQ=255)
          aGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCAtgg                                                                                                                                        >  2:851197/1‑150 (MQ=255)
           gTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCAtggt                                                                                                                                       >  1:1660892/1‑150 (MQ=255)
            ttATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCAtggtg                                                                                                                                      >  2:3657142/1‑150 (MQ=255)
             tATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCAtggtgg                                                                                                                                     <  1:3451725/150‑1 (MQ=255)
              aTTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTggg                                                                                                                                    <  1:3676424/150‑1 (MQ=255)
               ttGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTgggg                                                                                                                                   <  1:2898190/150‑1 (MQ=255)
                  cAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCt                                                                                                                                <  1:924573/150‑1 (MQ=255)
                  cAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCt                                                                                                                                <  2:614742/150‑1 (MQ=255)
                  cAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCt                                                                                                                                <  1:1146/150‑1 (MQ=255)
                   aGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTg                                                                                                                               <  1:2194261/150‑1 (MQ=255)
                    ggTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGa                                                                                                                              >  2:295119/1‑150 (MQ=255)
                       aaaGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGtt                                                                                                                           >  1:1191064/1‑150 (MQ=255)
                           ttttCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCAcc                                                                                                                       >  1:1514978/1‑150 (MQ=255)
                           ttttCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCAcc                                                                                                                       >  2:493638/1‑150 (MQ=255)
                            tttCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCg                                                                                                                      >  1:2808435/1‑150 (MQ=255)
                               cATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCggtg                                                                                                                   >  2:1752277/1‑150 (MQ=255)
                                 tGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCggtggt                                                                                                                 >  2:2816647/1‑150 (MQ=255)
                                   tttGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCa                                                                                                               <  2:2067452/150‑1 (MQ=255)
                                      gCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCa                                                                                                            >  2:1413355/1‑150 (MQ=255)
                                       ctctCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCAt                                                                                                           <  2:3340628/150‑1 (MQ=255)
                                         ctcGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCg                                                                                                         <  1:1807608/150‑1 (MQ=255)
                                         ctcGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCg                                                                                                         <  1:1715065/150‑1 (MQ=255)
                                         ctcGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCg                                                                                                         <  1:1593389/150‑1 (MQ=255)
                                         ctcGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCg                                                                                                         >  1:10380/1‑150 (MQ=255)
                                           cGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGAt                                                                                                       <  1:3190665/150‑1 (MQ=255)
                                              gtAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTcc                                                                                                    <  1:3358172/150‑1 (MQ=255)
                                              gtAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTcc                                                                                                    <  2:3501137/150‑1 (MQ=255)
                                                aGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCAt                                                                                                  >  2:1633933/1‑150 (MQ=255)
                                                 ggTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATc                                                                                                 <  1:299956/150‑1 (MQ=255)
                                                  gTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCt                                                                                                <  1:2034539/150‑1 (MQ=255)
                                                  gTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCt                                                                                                <  2:2418353/150‑1 (MQ=255)
                                                    aaTTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTgg                                                                                              >  2:2597958/1‑150 (MQ=255)
                                                    aaTTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTgg                                                                                              >  2:2196101/1‑150 (MQ=255)
                                                     aTTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTgga                                                                                             >  2:421359/1‑150 (MQ=255)
                                                        aaCGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTggagga                                                                                          >  2:882086/1‑150 (MQ=255)
                                                         aCGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGAt                                                                                         <  2:760582/150‑1 (MQ=255)
                                                          cGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATa                                                                                        <  2:2356608/150‑1 (MQ=255)
                                                          cGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATa                                                                                        >  2:1461270/1‑150 (MQ=255)
                                                           gCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATaa                                                                                       <  1:3653159/150‑1 (MQ=255)
                                                             tGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAg                                                                                     >  2:1045965/1‑150 (MQ=255)
                                                             tGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAg                                                                                     >  2:1827895/1‑150 (MQ=255)
                                                               aGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTg                                                                                   >  2:1553856/1‑150 (MQ=255)
                                                                   gggTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCggg                                                                               >  1:2546419/1‑150 (MQ=255)
                                                                    ggTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGa                                                                              <  2:2642256/150‑1 (MQ=255)
                                                                       aaaTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATg                                                                           <  2:3761138/150‑1 (MQ=255)
                                                                        aaTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGt                                                                          <  2:2395882/150‑1 (MQ=255)
                                                                         aTGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGtt                                                                         <  2:6565/150‑1 (MQ=255)
                                                                          tGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGttt                                                                        >  2:75084/1‑150 (MQ=255)
                                                                           ggCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTc                                                                       >  1:3174385/1‑150 (MQ=255)
                                                                           ggCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTc                                                                       >  1:2400765/1‑150 (MQ=255)
                                                                            gCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCa                                                                      <  2:1455984/150‑1 (MQ=255)
                                                                             cGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCAt                                                                     <  2:3067164/150‑1 (MQ=255)
                                                                              gATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATc                                                                    <  2:448420/150‑1 (MQ=255)
                                                                              gATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATc                                                                    >  2:1903440/1‑150 (MQ=255)
                                                                               aTGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATcc                                                                   <  2:2287113/150‑1 (MQ=255)
                                                                                    taCGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCtt                                                              <  1:3210909/150‑1 (MQ=255)
                                                                                      agTAGCCGCAACTGCCAAGGGTGTAGGCTTTGCATGTTAAATAGTTGTGGTACATGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTcc                                                            <  1:381791/149‑1 (MQ=255)
                                                                                         aGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGACTGTTTCATCCAGCTTCCGGc                                                         >  2:2795470/1‑150 (MQ=255)
                                                                                             gCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTc                                                     <  2:2780670/150‑1 (MQ=255)
                                                                                              cAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCg                                                    <  1:3207813/150‑1 (MQ=255)
                                                                                               aaCTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGa                                                   >  2:1849247/1‑150 (MQ=255)
                                                                                               aaCTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATCTTTCATCCAGCTTCCGGCATTCGa                                                   >  2:1138074/1‑150 (MQ=255)
                                                                                                aCTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGAt                                                  <  1:1533494/150‑1 (MQ=255)
                                                                                                aCTGCCAAGGGTGTCGGCATCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGAt                                                  >  1:1475593/1‑150 (MQ=255)
                                                                                                 cTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATg                                                 >  2:2552506/1‑150 (MQ=255)
                                                                                                  tGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGt                                                >  1:630654/1‑150 (MQ=255)
                                                                                                    ccAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCa                                              >  1:2151801/1‑150 (MQ=255)
                                                                                                      aatggTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACt                                            >  1:831409/4‑150 (MQ=255)
                                                                                                            gtCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACtt                                      >  2:3199020/1‑150 (MQ=255)
                                                                                                             tCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTg                                     <  2:530081/150‑1 (MQ=255)
                                                                                                             tCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTg                                     <  2:951552/150‑1 (MQ=255)
                                                                                                              cGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGc                                    <  2:1236767/150‑1 (MQ=255)
                                                                                                               ggCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGcc                                   >  2:2291279/1‑150 (MQ=255)
                                                                                                               ggCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGcc                                   <  1:2692492/150‑1 (MQ=255)
                                                                                                                    cGCAGGTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGa                              >  2:1205153/1‑150 (MQ=255)
                                                                                                                        ggTTAAATCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGTTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCt                          <  1:476068/150‑1 (MQ=255)
                                                                                                                            aaaTCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGcc                      >  1:2843521/1‑150 (MQ=255)
                                                                                                                             aaTCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGccc                     <  1:882086/150‑1 (MQ=255)
                                                                                                                               tCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCgg                   <  2:3514456/150‑1 (MQ=255)
                                                                                                                               tCGTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCgg                   <  1:421359/150‑1 (MQ=255)
                                                                                                                                 gTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTc                 <  2:115715/150‑1 (MQ=255)
                                                                                                                                 gTTGTGGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTc                 <  1:2551302/150‑1 (MQ=255)
                                                                                                                                   tgtgGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAg               >  2:1026562/1‑150 (MQ=255)
                                                                                                                                   tgtgGTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAg               <  1:452778/150‑1 (MQ=255)
                                                                                                                                      ggTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCa            <  1:645543/150‑1 (MQ=255)
                                                                                                                                      ggTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCa            <  1:1413355/150‑1 (MQ=255)
                                                                                                                                       gTACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGTAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAg           >  1:2240417/1‑150 (MQ=255)
                                                                                                                                        tACAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGa          >  2:1243781/1‑150 (MQ=255)
                                                                                                                                         aCAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGAt         <  2:2808435/150‑1 (MQ=255)
                                                                                                                                         aCAGGGTAACTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGAt         >  1:3368163/1‑150 (MQ=255)
                                                                                                                                                aaCTCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATGCAg  >  1:3555657/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGCCCGCTGAGTTATTGCAGGTAAAGTTTTCATGTTTGCTCTCGTGTAGGTAATTAACGCTGAGTGGGGTAAATGGCGATGTATACGTAGCCGCAACTGCCAAGGGTGTCGGCTTCGCAGGTTAAATCGTTGTGGTACAGGGTAACGCAGTGGGCATGGTGGGGGCTGAGTTCACCGGTGGTCAGCATCGATTCCATCTGGAGGATAAAGTGCGGGAATGTTTCATCCAGCTTCCGGCATTCGATGTCACTGAACTTGCCGGTGATGCTGGCCCGGTCAGCCAGATAATGCAG  >  CP009273/259347‑259640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑