Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,470,272 C→A V169V (GTG→GTT)  fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,470,2720CA99.6% 854.6 / NA 257V169V (GTG→GTT) fkpAFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (136/120);  total (136/121)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

AACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGC  >  CP009273/3470125‑3470418
                                                                                                                                                   |                                                                                                                                                  
aaCCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAAc                                                                                                                                                  >  1:812310/1‑150 (MQ=255)
aaCCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAAc                                                                                                                                                  >  2:353863/1‑150 (MQ=255)
aaCCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAAc                                                                                                                                                  >  2:3264678/1‑150 (MQ=255)
      tttGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTacaac                                                                                                                                            <  2:1267902/150‑1 (MQ=255)
          aTCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTa                                                                                                                                        <  1:1694783/150‑1 (MQ=255)
            cTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATc                                                                                                                                      <  2:200925/150‑1 (MQ=255)
             ttACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCg                                                                                                                                     >  1:2463047/1‑150 (MQ=255)
             ttACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCg                                                                                                                                     >  1:216041/1‑150 (MQ=255)
             ttACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCg                                                                                                                                     >  2:2615530/1‑150 (MQ=255)
             ttACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCg                                                                                                                                     <  1:1766203/150‑1 (MQ=255)
             ttACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAATATCg                                                                                                                                     >  1:3109783/1‑150 (MQ=255)
                  gCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTc                                                                                                                                >  1:3728199/1‑150 (MQ=255)
                   ccTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCt                                                                                                                               <  2:1002993/150‑1 (MQ=255)
                     tttCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCttt                                                                                                                             <  2:3757792/150‑1 (MQ=255)
                     tttCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCttt                                                                                                                             <  1:2867927/150‑1 (MQ=255)
                      ttCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTc                                                                                                                            <  1:2661024/150‑1 (MQ=255)
                       tCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCg                                                                                                                           <  2:558176/150‑1 (MQ=255)
                        cTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCgg                                                                                                                          >  2:367860/1‑150 (MQ=255)
                        cTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCgg                                                                                                                          <  2:691361/150‑1 (MQ=255)
                         ttGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGt                                                                                                                         >  2:955914/1‑150 (MQ=255)
                           gATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGc                                                                                                                       <  2:2512008/150‑1 (MQ=255)
                            aTGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCt                                                                                                                      >  1:2167953/1‑150 (MQ=255)
                            aTGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCt                                                                                                                      >  1:1926873/1‑150 (MQ=255)
                             tGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCtt                                                                                                                     >  2:1122580/1‑150 (MQ=255)
                                 cttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGcc                                                                                                                 >  2:1310971/1‑150 (MQ=255)
                                  ttcAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCt                                                                                                                >  1:1518191/1‑150 (MQ=255)
                                    cAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCttt                                                                                                              <  2:2791134/150‑1 (MQ=255)
                                    cAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCttt                                                                                                              >  2:3108577/1‑150 (MQ=255)
                                    cAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCttt                                                                                                              <  1:1043278/150‑1 (MQ=255)
                                     aGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTa                                                                                                             >  1:2630748/1‑150 (MQ=255)
                                      gACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTAc                                                                                                            <  1:2935096/150‑1 (MQ=255)
                                       aCCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTAcc                                                                                                           >  1:1831291/1‑150 (MQ=255)
                                        ccTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCg                                                                                                          >  2:2447804/1‑150 (MQ=255)
                                          ttCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGc                                                                                                        >  1:755546/1‑150 (MQ=255)
                                          ttCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGc                                                                                                        >  1:579271/1‑150 (MQ=255)
                                            cTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCtt                                                                                                      <  2:2959685/150‑1 (MQ=255)
                                            cTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCtt                                                                                                      <  2:2116858/150‑1 (MQ=255)
                                            cTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCtt                                                                                                      >  1:3792577/1‑150 (MQ=255)
                                              gTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTct                                                                                                    >  1:2540324/1‑150 (MQ=255)
                                              gTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTct                                                                                                    <  1:2581933/150‑1 (MQ=255)
                                               tCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTcta                                                                                                   >  1:1460016/1‑150 (MQ=255)
                                               tCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTcta                                                                                                   >  1:1148459/1‑150 (MQ=255)
                                                    cccGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTAcc                                                                                              <  1:2747446/150‑1 (MQ=255)
                                                    cccGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTAcc                                                                                              >  1:551416/1‑150 (MQ=255)
                                                     ccGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCt                                                                                             <  1:725957/150‑1 (MQ=255)
                                                          aTAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAATATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATaa                                                                                        >  1:1966188/1‑150 (MQ=255)
                                                           tAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATaaa                                                                                       >  2:2360523/1‑150 (MQ=255)
                                                            aaCACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAAAAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAac                                                                                      >  1:1658288/1‑150 (MQ=255)
                                                             acacCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAacc                                                                                     <  2:1155436/150‑1 (MQ=255)
                                                               acCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccag                                                                                   <  1:1122580/150‑1 (MQ=255)
                                                               acCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccag                                                                                   <  1:3382910/150‑1 (MQ=255)
                                                                 cGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagac                                                                                 <  2:2459807/150‑1 (MQ=255)
                                                                 cGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagac                                                                                 >  2:1847497/1‑150 (MQ=255)
                                                                  gTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTTTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAaccagacc                                                                                <  2:3583149/150‑1 (MQ=255)
                                                                     cAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGt                                                                             <  2:397047/150‑1 (MQ=255)
                                                                           gaaagaaaGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAaga                                                                       >  1:2818701/1‑150 (MQ=255)
                                                                            aaagaaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAagag                                                                      >  1:1578536/1‑150 (MQ=255)
                                                                             aagaaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAgg                                                                     >  2:3372499/1‑150 (MQ=255)
                                                                                aaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGttt                                                                  <  2:1113693/150‑1 (MQ=255)
                                                                                aaagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTGACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGttt                                                                  <  2:508722/150‑1 (MQ=255)
                                                                                 aagCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGtttt                                                                 >  2:2797483/1‑150 (MQ=255)
                                                                                  agCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTc                                                                <  2:812991/150‑1 (MQ=255)
                                                                                   gCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTca                                                               <  2:1393234/150‑1 (MQ=255)
                                                                                    cGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTcac                                                              >  2:579841/1‑150 (MQ=255)
                                                                                       ttCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACAcc                                                           >  2:1553450/1‑150 (MQ=255)
                                                                                         caccacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCtt                                                         >  2:1763027/1‑150 (MQ=255)
                                                                                          accacGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCttt                                                        <  1:852315/150‑1 (MQ=255)
                                                                                              cGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTctct                                                    <  1:1310971/150‑1 (MQ=255)
                                                                                              cGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTctct                                                    >  2:1144248/1‑150 (MQ=255)
                                                                                                 gtgtAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTg                                                 >  1:3027643/1‑150 (MQ=255)
                                                                                                     aaGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCaa                                             >  1:1254660/1‑150 (MQ=255)
                                                                                                        agTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAAtt                                          >  1:603497/1‑150 (MQ=255)
                                                                                                             tCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcg                                     >  2:1096875/1‑150 (MQ=255)
                                                                                                             tCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcg                                     <  1:955914/150‑1 (MQ=255)
                                                                                                              cGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcgc                                    >  1:2306595/1‑150 (MQ=255)
                                                                                                              cGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTcgc                                    >  1:2505844/1‑150 (MQ=255)
                                                                                                                 aCTCTTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGt                                 <  1:353863/150‑1 (MQ=255)
                                                                                                                    ctTTACCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCAGATAAACCAGACCCGTTGAAGAGGTTTTCACACCATACTCTTTGGCAAATTTCTCGCGGTAct                              >  1:3182828/1‑150 (MQ=255)
                                                                                                                        aCCGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCttt                          >  1:2343005/1‑150 (MQ=255)
                                                                                                                          cGTCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTAc                        >  1:2732167/1‑150 (MQ=255)
                                                                                                                            tCGATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCt                      <  2:1900470/150‑1 (MQ=255)
                                                                                                                              gATCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCttt                    <  1:3621496/150‑1 (MQ=255)
                                                                                                                               aTCAGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCtttt                   >  1:3087660/1‑150 (MQ=255)
                                                                                                                                  aGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCt                >  2:2721632/1‑150 (MQ=255)
                                                                                                                                  aGCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCt                <  1:2447804/150‑1 (MQ=255)
                                                                                                                                   gCGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCtt               <  2:3728199/150‑1 (MQ=255)
                                                                                                                                    cGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTc              >  2:88446/1‑150 (MQ=255)
                                                                                                                                    cGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTc              <  2:1701459/150‑1 (MQ=255)
                                                                                                                                    cGTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTc              >  1:2965563/1‑150 (MQ=255)
                                                                                                                                     gTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCg             <  2:2306595/150‑1 (MQ=255)
                                                                                                                                     gTACCTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCg             >  1:881248/1‑150 (MQ=255)
                                                                                                                                        ccTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTa          >  2:3486826/1‑150 (MQ=255)
                                                                                                                                        ccTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTa          <  1:1036428/150‑1 (MQ=255)
                                                                                                                                         cTTTGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTAt         <  2:2177321/150‑1 (MQ=255)
                                                                                                                                          tttGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATc        >  2:1796052/1‑150 (MQ=255)
                                                                                                                                           ttGTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCa       <  1:1538095/150‑1 (MQ=255)
                                                                                                                                             gTAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGc     <  1:3459666/150‑1 (MQ=255)
                                                                                                                                              tAGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGcc    >  1:650867/1‑150 (MQ=255)
                                                                                                                                               aGTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCcg   <  1:833852/150‑1 (MQ=255)
                                                                                                                                                gTTAACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCcgc  >  2:290827/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCAGACCTTCTGTCCAACCCGGGATAACACCGTCCAGACGGAAAGAAAGCGGTTCACCACGGGTGTAAGAGTTGTCGAACTCTTTACCGTCGATCAGCGTACCTTTGTAGTTCACTACAACAGTATCGCTGTCTTTCGGTGCTTCGCCTTTACCGGCTTCTACTACCTGATAAACCAGACCAGTTGAAGAGGTTTTCACACCTTTCTCTTTGGCAAATTTCTCGCGGTACTCTTTACCTTTTGCTTCGTTATCAGCCGC  >  CP009273/3470125‑3470418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑