BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,479,769	A→T	E97D (GAA→GAT)	crp →	cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,479,769	0	A	T	100.0%	874.1 / NA	260	E97D (GAA→GAT)	crp	cAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (137/123); total (137/123)

Only 100 of 263 total aligned reads displayed.

GGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCA  >  CP009273/3479622‑3479913

ggCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGatat                                                                                                                                                <  2:1813137/150‑1 (MQ=255)

  cAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATAttt                                                                                                                                              >  1:3055046/1‑150 (MQ=255)

  cAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATAttt                                                                                                                                              <  2:169502/150‑1 (MQ=255)

   aGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTc                                                                                                                                             <  2:2346966/150‑1 (MQ=255)

    gTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCg                                                                                                                                            <  1:1955677/150‑1 (MQ=255)

    gTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCGATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCg                                                                                                                                            <  2:1085863/150‑1 (MQ=255)

      gCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTa                                                                                                                                          <  1:1594594/150‑1 (MQ=255)

        tGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACa                                                                                                                                        >  1:726875/1‑150 (MQ=255)

        tGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACa                                                                                                                                        >  2:3212488/1‑150 (MQ=255)

          aTCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaa                                                                                                                                      >  1:1318913/1‑150 (MQ=255)

          aTCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaa                                                                                                                                      <  2:936164/150‑1 (MQ=255)

          aTCAAAGACGAAGAGGGTAAAGAAATGATACTCTCCTATCTGAATCAGGGTGATTTTATTGTCGAACTGATCCTGTTTGAAGAGGGCCAGTAACGTAGCGCATGGGTACGTGCGAAAACCGCCTATGAAGTGGCTGATATTTCGTACaaa                                                                                                                                      >  1:2048503/1‑150 (MQ=255)

           tCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaaa                                                                                                                                     <  2:571054/150‑1 (MQ=255)

            cAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaaaa                                                                                                                                    >  2:2710045/1‑150 (MQ=255)

             aaaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaaaaa                                                                                                                                   <  1:3344510/150‑1 (MQ=255)

                 aCGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTc                                                                                                                               <  1:1985657/150‑1 (MQ=255)

                     agagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCa                                                                                                                           >  1:2222621/1‑150 (MQ=255)

                      gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCaa                                                                                                                          <  2:3342752/150‑1 (MQ=255)

                      gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCaa                                                                                                                          <  2:3226202/150‑1 (MQ=255)

                      gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCaa                                                                                                                          >  1:974613/1‑150 (MQ=255)

                      gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCaa                                                                                                                          >  1:974516/1‑150 (MQ=255)

                       agGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAAt                                                                                                                         <  2:2612213/150‑1 (MQ=255)

                       agGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAAt                                                                                                                         <  2:705109/150‑1 (MQ=255)

                       agGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAAt                                                                                                                         <  1:3519469/150‑1 (MQ=255)

                              aGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCa                                                                                                                  <  1:3369404/150‑1 (MQ=255)

                              aGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCa                                                                                                                  <  1:2641881/150‑1 (MQ=255)

                               gAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAg                                                                                                                 <  2:1375011/150‑1 (MQ=255)

                                aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAgg                                                                                                                <  1:355641/150‑1 (MQ=255)

                                aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAgg                                                                                                                >  2:3598347/1‑150 (MQ=255)

                                aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAgg                                                                                                                <  2:862051/150‑1 (MQ=255)

                                  aTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa                                                                                                              >  1:258161/1‑150 (MQ=255)

                                  aTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa                                                                                                              >  2:2898114/1‑150 (MQ=255)

                                  aTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa                                                                                                              >  1:211820/1‑150 (MQ=255)

                                      tCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAcc                                                                                                          <  2:3444254/150‑1 (MQ=255)

                                       ccTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                                                                         <  1:2335447/150‑1 (MQ=255)

                                       ccTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAccc                                                                                                         >  1:1689182/1‑150 (MQ=255)

                                             ctgtCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                   >  1:2543479/4‑150 (MQ=255)

                                             cTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                   <  2:537298/150‑1 (MQ=255)

                                             cTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                   <  2:1597489/150‑1 (MQ=255)

                                              tctCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                                                                  <  1:2679084/148‑1 (MQ=255)

                                               aTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                 >  2:948592/1‑150 (MQ=255)

                                               aTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                 >  2:144532/1‑150 (MQ=255)

                                                 cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTg                                                                                               <  1:238045/150‑1 (MQ=255)

                                                   gAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAt                                                                                             <  1:1372038/150‑1 (MQ=255)

                                                   gAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAt                                                                                             >  2:2848109/1‑150 (MQ=255)

                                                       cAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                                                                         >  2:3520704/1‑150 (MQ=255)

                                                       cAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCCCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt                                                                                         >  2:212090/1‑150 (MQ=255)

                                                         gggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                                                                       <  1:428424/150‑1 (MQ=255)

                                                          ggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTg                                                                                      >  2:1220323/1‑150 (MQ=255)

                                                            tGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTc                                                                                    <  1:2934676/150‑1 (MQ=255)

                                                            tGATTTTATTGACGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTc                                                                                    >  2:3149623/1‑150 (MQ=255)

                                                             gATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCt                                                                                   <  1:936593/150‑1 (MQ=255)

                                                              aTTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTg                                                                                  >  2:817156/1‑150 (MQ=255)

                                                                  tATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcaca                                                                              <  2:2454307/150‑1 (MQ=255)

                                                                   aTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAg                                                                             >  2:1054153/1‑150 (MQ=255)

                                                                    ttGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGa                                                                            >  1:1549466/1‑150 (MQ=255)

                                                                     tGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                                                                           >  2:2813709/1‑150 (MQ=255)

                                                                     tGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                                                                           <  2:469280/150‑1 (MQ=255)

                                                                      ggCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATg                                                                          <  2:3773165/150‑1 (MQ=255)

                                                                         gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcg                                                                       >  1:1156409/1‑150 (MQ=255)

                                                                           aCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGcgcg                                                                     <  2:432558/150‑1 (MQ=255)

                                                                                 ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt                                                               >  1:1244475/1‑150 (MQ=255)

                                                                                    gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCa                                                            >  1:2439371/1‑150 (MQ=255)

                                                                                       tGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGt                                                         >  1:1867926/1‑150 (MQ=255)

                                                                                           gagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt                                                     <  1:1026729/150‑1 (MQ=255)

                                                                                            agGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt                                                    >  2:3384804/1‑150 (MQ=255)

                                                                                             gggCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTc                                                   <  2:3187403/150‑1 (MQ=255)

                                                                                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCaga                                                <  1:144532/150‑1 (MQ=255)

                                                                                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCaga                                                >  2:1927713/1‑150 (MQ=255)

                                                                                                ccAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACATCaga                                                >  2:1941558/1‑150 (MQ=255)

                                                                                                 cAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCagag                                               >  1:3136506/1‑150 (MQ=255)

                                                                                                  aGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAgaga                                              >  1:1012607/1‑150 (MQ=255)

                                                                                                    gAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGaaa                                            <  1:570493/150‑1 (MQ=255)

                                                                                                     aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAg                                           >  1:1298966/1‑150 (MQ=255)

                                                                                                     aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAg                                           >  1:82157/1‑150 (MQ=255)

                                                                                                      aCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGt                                          <  1:1622394/150‑1 (MQ=255)

                                                                                                          aGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGc                                      <  2:3420187/150‑1 (MQ=255)

                                                                                                            cgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCaa                                    >  1:2348348/1‑150 (MQ=255)

                                                                                                              cATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAAcc                                  >  1:3199233/1‑150 (MQ=255)

                                                                                                               aTGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCt                                 <  1:1059811/150‑1 (MQ=255)

                                                                                                                tGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTg                                <  2:1318913/150‑1 (MQ=255)

                                                                                                                   gTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCg                             <  1:2205799/150‑1 (MQ=255)

                                                                                                                    tACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGt                            >  2:909996/1‑150 (MQ=255)

                                                                                                                         gCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATGTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTc                       <  2:1475674/150‑1 (MQ=255)

                                                                                                                            aaaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc                    <  2:2763415/150‑1 (MQ=255)

                                                                                                                            aaaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc                    >  2:1199818/1‑150 (MQ=255)

                                                                                                                            aaaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc                    <  1:1424351/150‑1 (MQ=255)

                                                                                                                              aaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGt                  >  1:3354702/1‑150 (MQ=255)

                                                                                                                                 cGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGAc               <  2:2543479/150‑1 (MQ=255)

                                                                                                                                 cGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGAc               >  2:2186180/1‑150 (MQ=255)

                                                                                                                                    cTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACggg            >  1:820139/1‑150 (MQ=255)

                                                                                                                                     tgtgAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGc           >  1:2974099/1‑150 (MQ=255)

                                                                                                                                     tgtgAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGc           >  1:1400341/1‑150 (MQ=255)

                                                                                                                                         aaGTGGCTGATATTTCGTACAAATAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCa       <  2:3055046/150‑1 (MQ=255)

                                                                                                                                         aaGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCa       >  2:1748916/1‑150 (MQ=255)

                                                                                                                                            tGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg    <  2:974516/150‑1 (MQ=255)

                                                                                                                                            tGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg    <  1:2020317/150‑1 (MQ=255)

                                                                                                                                            tGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg    >  2:2337920/1‑150 (MQ=255)

                                                                                                                                            tGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg    >  2:2220303/1‑150 (MQ=255)

                                                                                                                                              gCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGca  <  1:3384804/150‑1 (MQ=255)

GGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCA  >  CP009273/3479622‑3479913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`