Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,538,583 C→A R115S (CGC→AGC)  gntX → DNA catabolic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,538,5830CA99.6% 818.2 / ‑5.0 245R115S (CGC→AGC) gntXDNA catabolic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (129/115);  minor base T (0/1);  total (129/116)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 245 total aligned reads displayed.

GTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGACCCAG  >  CP009273/3538439‑3538729
                                                                                                                                                |                                                                                                                                                  
 ttGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCg                                                                                                                                              <  2:2993738/150‑1 (MQ=255)
   gCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTc                                                                                                                                            <  1:2138576/150‑1 (MQ=255)
      gACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGc                                                                                                                                         >  1:1247951/1‑150 (MQ=255)
      gACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGc                                                                                                                                         <  1:2620132/150‑1 (MQ=255)
      gACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGc                                                                                                                                         >  1:2314414/1‑150 (MQ=255)
       aCTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCg                                                                                                                                        >  1:1231742/1‑150 (MQ=255)
         tATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGtt                                                                                                                                      <  2:1163696/150‑1 (MQ=255)
         tATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGtt                                                                                                                                      <  2:2642438/150‑1 (MQ=255)
              gccgccGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGtt                                                                                                                                 <  2:2996505/150‑1 (MQ=255)
               ccgccgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTa                                                                                                                                >  1:852114/1‑150 (MQ=255)
                 gccgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATg                                                                                                                              >  1:2056182/1‑150 (MQ=255)
                 gccgTTAAGTCCGCCTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATg                                                                                                                              <  1:2891367/150‑1 (MQ=255)
                  ccgTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATgg                                                                                                                             >  2:2597671/1‑150 (MQ=255)
                      tAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAgc                                                                                                                         >  1:1834191/1‑150 (MQ=255)
                       aaGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAgcg                                                                                                                        >  2:1844721/1‑150 (MQ=255)
                       aaGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAgcg                                                                                                                        >  1:1528798/1‑150 (MQ=255)
                             gCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCa                                                                                                                  >  1:1716900/1‑150 (MQ=255)
                              cTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCAc                                                                                                                 <  1:3641521/150‑1 (MQ=255)
                                tATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTg                                                                                                               <  2:2522599/150‑1 (MQ=255)
                                  tCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGc                                                                                                             >  1:668863/1‑150 (MQ=255)
                                     accaGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTc                                                                                                          <  1:3080656/150‑1 (MQ=255)
                                      ccaGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCg                                                                                                         <  2:3051612/150‑1 (MQ=255)
                                        aGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCggg                                                                                                       <  1:3000925/150‑1 (MQ=255)
                                         gCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCgggg                                                                                                      >  2:97426/1‑150 (MQ=255)
                                         gCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCgggg                                                                                                      >  2:799626/1‑150 (MQ=255)
                                             aaaTTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGAttt                                                                                                  <  1:1336726/150‑1 (MQ=255)
                                             aaaTTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGAttt                                                                                                  <  1:498273/150‑1 (MQ=255)
                                              aaTTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTa                                                                                                 >  1:2144555/1‑150 (MQ=255)
                                              aaTTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTa                                                                                                 >  1:2253399/1‑150 (MQ=255)
                                              aaTTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTa                                                                                                 <  1:3652736/150‑1 (MQ=255)
                                               aTTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTaa                                                                                                >  1:663508/1‑150 (MQ=255)
                                               aTTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTaa                                                                                                >  1:3269425/1‑150 (MQ=255)
                                                ttttCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAAt                                                                                               >  1:194546/1‑150 (MQ=255)
                                                 tttCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATc                                                                                              <  2:1033405/150‑1 (MQ=255)
                                                  ttCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCa                                                                                             >  2:3582239/1‑150 (MQ=255)
                                                  gtCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCa                                                                                             <  1:2066599/149‑1 (MQ=255)
                                                  gtCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCa                                                                                             <  2:3157107/149‑1 (MQ=255)
                                                     ccGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCagag                                                                                          <  2:2314414/150‑1 (MQ=255)
                                                     ccGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCagag                                                                                          <  2:406840/150‑1 (MQ=255)
                                                       ggCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCg                                                                                        >  2:941066/1‑150 (MQ=255)
                                                       ggCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCg                                                                                        >  2:1586351/1‑150 (MQ=255)
                                                        gcgcAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGa                                                                                       <  2:3511793/150‑1 (MQ=255)
                                                          gcAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGAtt                                                                                     >  1:389536/1‑150 (MQ=255)
                                                           cAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGAttt                                                                                    <  2:220796/150‑1 (MQ=255)
                                                            aGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTg                                                                                   >  2:2367848/1‑150 (MQ=255)
                                                             gTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGc                                                                                  >  2:2715196/1‑150 (MQ=255)
                                                             gTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGc                                                                                  >  2:3531358/1‑150 (MQ=255)
                                                               gAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCtg                                                                                >  2:2226878/1‑150 (MQ=255)
                                                                 aaTCGCCAGCTCCCTGTTTTGTTTGTTACTATTGGATGTCTTAGACGCTTGTCGCACCTCCGGGTTGGAATTGGCGGTTTGGATCGTCAGCGTTCAGTTATGGCAGCGGCGTCACTGGCGTCGGTGATTTAATCAGAGCGATTTGCtgtg                                                                              <  1:485104/150‑1 (MQ=255)
                                                                    cGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCa                                                                           <  1:1423980/150‑1 (MQ=255)
                                                                    cGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCa                                                                           <  2:3747321/150‑1 (MQ=255)
                                                                       cAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGcc                                                                        >  1:3716150/1‑150 (MQ=255)
                                                                        aGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCg                                                                       >  2:1667486/1‑150 (MQ=255)
                                                                            cccTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTAt                                                                   >  2:1762619/1‑150 (MQ=255)
                                                                               tGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCAc                                                                >  1:107885/1‑150 (MQ=255)
                                                                               tGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCAc                                                                >  2:710755/1‑150 (MQ=255)
                                                                               tGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCAc                                                                <  2:2347815/150‑1 (MQ=255)
                                                                                 tCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGc                                                              >  2:1468602/1‑150 (MQ=255)
                                                                                 tCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGc                                                              >  2:3652594/1‑150 (MQ=255)
                                                                                  cACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCt                                                             >  2:3146868/1‑150 (MQ=255)
                                                                                   aCGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTg                                                            <  2:1779232/150‑1 (MQ=255)
                                                                                     gTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGt                                                          <  2:282954/150‑1 (MQ=255)
                                                                                           tACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACt                                                    <  2:2518277/150‑1 (MQ=255)
                                                                                            aCTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTg                                                   <  1:2597671/150‑1 (MQ=255)
                                                                                                ttGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCaa                                               <  1:935943/150‑1 (MQ=255)
                                                                                                 tGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAAt                                              <  1:2944386/150‑1 (MQ=255)
                                                                                                  ggAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATg                                             <  2:3716150/150‑1 (MQ=255)
                                                                                                   gAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATgg                                            >  2:188553/1‑150 (MQ=255)
                                                                                                      gTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGAt                                         >  2:2385949/1‑150 (MQ=255)
                                                                                                          tACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGca                                     <  2:663508/150‑2 (MQ=255)
                                                                                                           acacGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGa                                    <  2:2132336/150‑1 (MQ=255)
                                                                                                            cacGCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGaa                                   >  2:2409826/1‑150 (MQ=255)
                                                                                                               gCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGcc                                <  2:1016665/150‑1 (MQ=255)
                                                                                                               gCTCGTCGCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGAGTCAGCCGTTATCACGCTGGTTGCACTGCCACTGGGATAGCGAAGcc                                >  1:2015798/1‑150 (MQ=255)
                                                                                                                  cgtcgCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTc                             <  2:3611332/150‑1 (MQ=255)
                                                                                                                  cgtcgCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTc                             >  2:170609/1‑150 (MQ=255)
                                                                                                                   gtcgCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCACTGGGATAGCGAAGCCGTca                            >  1:3554411/1‑150 (MQ=255)
                                                                                                                   gtcgCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTca                            <  2:3435435/150‑1 (MQ=255)
                                                                                                                    tcgCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTcac                           >  1:1665305/1‑150 (MQ=255)
                                                                                                                     cgCACCACCGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTcaca                          >  2:549917/1‑150 (MQ=255)
                                                                                                                        accaccGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGt                       >  1:2681303/1‑150 (MQ=255)
                                                                                                                         ccaccGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTa                      >  1:1953186/1‑150 (MQ=255)
                                                                                                                          caccGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTac                     >  2:1991177/1‑150 (MQ=255)
                                                                                                                            ccGGGTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTacac                   <  1:355737/150‑1 (MQ=255)
                                                                                                                              gggTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACgg                 <  2:1933569/150‑1 (MQ=255)
                                                                                                                                gTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTaca                    >  1:3513840/1‑145 (MQ=255)
                                                                                                                                gTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGc               >  1:2816251/1‑150 (MQ=255)
                                                                                                                                gTTGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGc               >  1:2731082/1‑150 (MQ=255)
                                                                                                                                 ttGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGcc              <  2:1188295/150‑1 (MQ=255)
                                                                                                                                 ttGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGcc              <  2:2442065/150‑1 (MQ=255)
                                                                                                                                 ttGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGcc              <  2:2511630/150‑1 (MQ=255)
                                                                                                                                 ttGCAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGcc              <  2:3088545/150‑1 (MQ=255)
                                                                                                                                    cAATTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGTATAGCGAAGCCGTCACACGTACACGGGCCACt           >  1:3401611/1‑150 (MQ=255)
                                                                                                                                     aaTTGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTg          <  1:3373201/150‑1 (MQ=255)
                                                                                                                                       ttGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCg        <  2:908358/150‑1 (MQ=255)
                                                                                                                                        tGCCGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGa       >  2:3208375/1‑150 (MQ=255)
                                                                                                                                           cGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGAccc    >  1:657876/1‑150 (MQ=255)
                                                                                                                                           cGGATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGAccc    <  1:3303475/150‑1 (MQ=255)
                                                                                                                                             gATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGACCCAg  >  2:1481674/1‑150 (MQ=255)
                                                                                                                                             gATAGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGACCCAg  >  2:3379987/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAGCTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCACCACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGAGCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACTGCGACCCAG  >  CP009273/3538439‑3538729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑