BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,566,100	G→T	R255S (CGC→AGC)	glgB ←	1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,566,100	0	G	T	100.0%	803.8 / NA	240	R255S (CGC→AGC)	glgB	1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (124/116); total (124/116)

Only 100 of 240 total aligned reads displayed.

GGTTGGCTGATAACCCCAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCGACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCTTCAAAGGCA  >  CP009273/3565954‑3566245

ggTTGGCTGATAACCCCAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACg                                                                                                                                                >  1:201420/1‑150 (MQ=255)

ggTTGGCTGATAACCCCAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACg                                                                                                                                                >  1:1789436/1‑150 (MQ=255)

   tGGCTGATAACCCCAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCa                                                                                                                                             >  2:56047/1‑150 (MQ=255)

      cTGATAACCCCAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGa                                                                                                                                          >  2:2236039/1‑150 (MQ=255)

       tGATAACCCCAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGaa                                                                                                                                         >  2:3789672/1‑150 (MQ=255)

          tAACCCCAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGTTCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAAccc                                                                                                                                      <  2:1716652/150‑1 (MQ=255)

            aCCCCAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAg                                                                                                                                    >  2:2459007/1‑150 (MQ=255)

              cccAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGt                                                                                                                                  >  1:1520142/1‑150 (MQ=255)

              cccAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGt                                                                                                                                  <  1:3186712/150‑1 (MQ=255)

                cAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGa                                                                                                                                <  1:2352364/150‑1 (MQ=255)

                    tGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACtt                                                                                                                            >  1:2452668/1‑150 (MQ=255)

                    tGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGATGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACtt                                                                                                                            >  1:2452768/1‑150 (MQ=255)

                           gAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAAt                                                                                                                     >  2:1923095/1‑150 (MQ=255)

                             aGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATag                                                                                                                   <  2:1177420/150‑1 (MQ=255)

                             aGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATag                                                                                                                   >  2:309066/1‑150 (MQ=255)

                             aGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATag                                                                                                                   >  2:1490085/1‑150 (MQ=255)

                             aGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATag                                                                                                                   >  2:1286903/1‑150 (MQ=255)

                             aGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATag                                                                                                                   <  1:1939873/150‑1 (MQ=255)

                              gggATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATaga                                                                                                                  <  1:1441691/150‑1 (MQ=255)

                                gATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAgaga                                                                                                                <  1:2170948/150‑1 (MQ=255)

                                 aTGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGAt                                                                                                               <  1:3225447/150‑1 (MQ=255)

                                 aTGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGAt                                                                                                               <  1:760558/150‑1 (MQ=255)

                                    cTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTgg                                                                                                            <  1:1998973/150‑1 (MQ=255)

                                     tCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGgc                                                                                                           <  2:2915233/150‑1 (MQ=255)

                                     tCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGgc                                                                                                           >  1:1757577/1‑150 (MQ=255)

                                           aTGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCa                                                                                                     <  1:1981085/150‑1 (MQ=255)

                                            tGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGACCCCAGGTGAACTTCATAAATAGAGATTGGCGCATCaa                                                                                                    >  1:3476769/1‑150 (MQ=255)

                                            tGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCaa                                                                                                    >  2:2539116/1‑150 (MQ=255)

                                               gCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACt                                                                                                 <  1:3316075/150‑1 (MQ=255)

                                                  gtagtTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGAt                                                                                              >  1:3643084/1‑150 (MQ=255)

                                                  gtagtTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGAt                                                                                              <  2:2285184/150‑1 (MQ=255)

                                                   tagtTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGAtt                                                                                             <  2:3106863/150‑1 (MQ=255)

                                                    agtTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTc                                                                                            >  2:1538661/1‑150 (MQ=255)

                                                     gtTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCg                                                                                           >  1:368102/1‑150 (MQ=255)

                                                       tCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCt                                                                                         <  1:1891582/150‑1 (MQ=255)

                                                         gAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCttt                                                                                       >  1:2681277/1‑150 (MQ=255)

                                                          aGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCtttt                                                                                      >  2:482286/1‑150 (MQ=255)

                                                          aGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCtttt                                                                                      >  1:2128883/1‑150 (MQ=255)

                                                          aGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCtttt                                                                                      <  1:1699721/150‑1 (MQ=255)

                                                            gTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCtttttt                                                                                    <  1:2193687/150‑1 (MQ=255)

                                                            gTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCtttttt                                                                                    <  1:3769203/150‑1 (MQ=255)

                                                             tGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTg                                                                                   >  1:313096/1‑150 (MQ=255)

                                                             tGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTg                                                                                   >  1:314729/1‑150 (MQ=255)

                                                             tGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTg                                                                                   >  1:1840683/1‑150 (MQ=255)

                                                               ggTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTgcg                                                                                 >  2:26730/1‑150 (MQ=255)

                                                                   aaGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGctct                                                                             >  2:2859395/1‑150 (MQ=255)

                                                                    aGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCtt                                                                            >  2:2490217/1‑150 (MQ=255)

                                                                    aGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCtt                                                                            >  2:2009637/1‑150 (MQ=255)

                                                                       ccatccatTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAg                                                                         >  1:1174464/1‑150 (MQ=255)

                                                                       ccatccatTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAg                                                                         <  2:1963217/150‑1 (MQ=255)

                                                                        catccatTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGt                                                                        >  2:1617399/1‑150 (MQ=255)

                                                                          tccatTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCt                                                                      <  1:106054/150‑1 (MQ=255)

                                                                            catTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGt                                                                    >  1:2321948/1‑150 (MQ=255)

                                                                             atTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTa                                                                   <  2:2765602/150‑1 (MQ=255)

                                                                             atTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTa                                                                   <  1:293658/150‑1 (MQ=255)

                                                                               ttAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACa                                                                 <  1:565705/150‑1 (MQ=255)

                                                                                tAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACaa                                                                >  1:3491708/1‑150 (MQ=255)

                                                                                 aGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAAc                                                               <  1:2834314/150‑1 (MQ=255)

                                                                                   cATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCt                                                             >  2:781587/1‑150 (MQ=255)

                                                                                      aaGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCtttt                                                          <  2:735636/150‑1 (MQ=255)

                                                                                       aGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTc                                                         <  2:2110828/150‑1 (MQ=255)

                                                                                       aGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTc                                                         <  2:201420/150‑1 (MQ=255)

                                                                                        ggCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTcc                                                        <  2:3643973/150‑1 (MQ=255)

                                                                                        ggCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTcc                                                        <  1:1617399/150‑1 (MQ=255)

                                                                                            ccAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCa                                                    <  2:888471/150‑1 (MQ=255)

                                                                                              aGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGc                                                  >  1:2833279/1‑150 (MQ=255)

                                                                                              aGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGc                                                  >  2:2595713/1‑150 (MQ=255)

                                                                                                      ggCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAAt                                          <  2:1127609/150‑1 (MQ=255)

                                                                                                      ggCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAAt                                          >  2:2080880/1‑150 (MQ=255)

                                                                                                      ggCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAAt                                          <  1:486800/150‑1 (MQ=255)

                                                                                                         cAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAag                                       >  1:3066815/1‑150 (MQ=255)

                                                                                                          aGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAaga                                      >  2:3566026/1‑150 (MQ=255)

                                                                                                            cTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAgaga                                    <  2:640246/150‑1 (MQ=255)

                                                                                                             tCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGAc                                   <  1:2060373/150‑1 (MQ=255)

                                                                                                                cgGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGAcgcg                                <  2:1520142/150‑1 (MQ=255)

                                                                                                                cgGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGAcgcg                                <  2:422153/150‑1 (MQ=255)

                                                                                                                cgGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGAcgcg                                >  2:3065296/1‑150 (MQ=255)

                                                                                                                cgGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGAcgcg                                >  1:920983/1‑150 (MQ=255)

                                                                                                                  gTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGt                              <  1:1287006/150‑1 (MQ=255)

                                                                                                                  gTAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGt                              <  2:2452699/150‑1 (MQ=255)

                                                                                                                   tAGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCTGGCAGCCCGCAAATAAGAGACGCGGtt                             <  2:2714987/150‑1 (MQ=255)

                                                                                                                    aGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGttt                            >  2:7260/1‑150 (MQ=255)

                                                                                                                    aGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGttt                            <  1:1744438/150‑1 (MQ=255)

                                                                                                                    aGCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGttt                            <  2:1180575/150‑1 (MQ=255)

                                                                                                                     gCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTc                           <  1:3081884/150‑1 (MQ=255)

                                                                                                                     gCTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTc                           >  1:2550500/1‑150 (MQ=255)

                                                                                                                      cTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTcc                          <  1:595132/150‑1 (MQ=255)

                                                                                                                      cTCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTcc                          <  1:3581023/150‑1 (MQ=255)

                                                                                                                       tCAACCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCg                         <  1:1444203/150‑1 (MQ=255)

                                                                                                                         aaCCAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCggg                       >  1:1379457/1‑150 (MQ=255)

                                                                                                                           ccAGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGgcg                     <  2:21840/150‑1 (MQ=255)

                                                                                                                             aGAAATTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCa                   >  1:148460/1‑150 (MQ=255)

                                                                                                                               aaaTTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCAtt                 <  1:1012112/150‑1 (MQ=255)

                                                                                                                                 aTTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTg               >  1:2381380/1‑150 (MQ=255)

                                                                                                                                 aTTGTTGTCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTg               <  2:1297955/150‑1 (MQ=255)

                                                                                                                                    gttgtCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTgcgc            >  2:846292/1‑150 (MQ=255)

                                                                                                                                      tgtCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCtt          <  1:1553272/150‑1 (MQ=255)

                                                                                                                                      tgtCGGTGTGGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGCCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCtt          <  2:1789436/150‑1 (MQ=255)

                                                                                                                                            tgtgGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCTTCAAAgg    <  1:2539116/150‑1 (MQ=255)

                                                                                                                                             gtgGCTACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCTTCAAAGGc   >  1:3480742/1‑150 (MQ=255)

GGTTGGCTGATAACCCCAACTGCCATCGAAGGGATGCTCGTTAATGGGCAGTAGTTCGAGGTGGGTAAAGCCCATCCATTTAGCATAAGGCACCAGTTGATCGGCCAGCTCGCGGTAGCTCAACCAGAAATTGTTGTCGGTGTGGCGACGCCAGGAACCCAGGTGAACTTCATAAATAGAGATTGGCGCATCAAACTGATTCGCTTTTTTGCGCTCTTCAGTCTGTACAACCTTTTCCGGCAGCCCGCAAATAAGAGACGCGGTTTCCGGGCGCATTTGCGCTTCAAAGGCA  >  CP009273/3565954‑3566245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`