Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,607,110 C→A I28I (ATC→ATA)  nikA → nickel‑binding, heme‑binding periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,607,1100CA100.0% 759.0 / NA 230I28I (ATC→ATA) nikAnickel‑binding, heme‑binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (121/109);  total (121/109)

Only 100 of 235 total aligned reads displayed.

TATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGAC  >  CP009273/3606963‑3607256
                                                                                                                                                   |                                                                                                                                                  
  tGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacca                                                                                                                                                <  1:2320672/150‑1 (MQ=255)
   gACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaaccac                                                                                                                                               >  2:713061/1‑150 (MQ=255)
     cGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCg                                                                                                                                             >  1:1126235/1‑150 (MQ=255)
       aaTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCTTTGGGGTTACCATGCTCTCCACACTCCGCCGAACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                           <  1:3178243/150‑1 (MQ=255)
       aaTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                           >  1:584296/1‑150 (MQ=255)
           cTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGc                                                                                                                                       >  2:3282929/1‑150 (MQ=255)
           cTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGc                                                                                                                                       <  2:2997835/150‑1 (MQ=255)
               aaaTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGt                                                                                                                                   >  2:3230089/1‑150 (MQ=255)
               aaaTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGt                                                                                                                                   <  1:2873533/150‑1 (MQ=255)
                aaTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTg                                                                                                                                  >  2:3116047/1‑150 (MQ=255)
                   cGTCATACTTATTTCCGCCATCTATTTTTATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAAt                                                                                                                               <  1:3219855/150‑1 (MQ=255)
                   cGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAAt                                                                                                                               <  1:2572147/150‑1 (MQ=255)
                   cGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAAt                                                                                                                               >  2:1060572/1‑150 (MQ=255)
                    gTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATg                                                                                                                              <  1:1500510/150‑1 (MQ=255)
                     tCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGt                                                                                                                             >  1:325600/1‑150 (MQ=255)
                      cATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTc                                                                                                                            >  1:2097317/1‑150 (MQ=255)
                        tACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCgg                                                                                                                          <  1:1759979/150‑1 (MQ=255)
                         aCTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCggg                                                                                                                         >  1:1335949/1‑150 (MQ=255)
                          cTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                        >  1:3036490/1‑150 (MQ=255)
                          cTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                        >  1:2963725/1‑150 (MQ=255)
                          cTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                        >  1:1061339/1‑150 (MQ=255)
                           ttATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGcc                                                                                                                       >  1:3322780/1‑150 (MQ=255)
                            tATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCa                                                                                                                      >  1:2545703/1‑150 (MQ=255)
                             aTTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCAc                                                                                                                     >  1:1366932/1‑150 (MQ=255)
                              tttCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACt                                                                                                                    >  2:1357609/1‑150 (MQ=255)
                                    ccATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAccc                                                                                                              >  2:1572856/1‑150 (MQ=255)
                                     cATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCg                                                                                                             >  2:3089121/1‑150 (MQ=255)
                                           ttttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCtt                                                                                                       >  2:3492553/1‑150 (MQ=255)
                                            tttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCttt                                                                                                      <  1:2160358/150‑1 (MQ=255)
                                              tAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTac                                                                                                    >  2:492594/1‑150 (MQ=255)
                                               aaTCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTaca                                                                                                   >  2:2226869/1‑150 (MQ=255)
                                                aTCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTacac                                                                                                  <  1:3117410/150‑1 (MQ=255)
                                                 tCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACg                                                                                                 <  1:432259/150‑1 (MQ=255)
                                                    aTTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCt                                                                                              <  1:780035/150‑1 (MQ=255)
                                                    aTTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCt                                                                                              >  2:2420279/1‑150 (MQ=255)
                                                     ttGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGACTGTCGGGCCACTAAACCCGCACCTTTACACGCCTa                                                                                             >  1:1869781/1‑150 (MQ=255)
                                                           ttACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCTATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGa                                                                                       >  1:1207712/1‑150 (MQ=255)
                                                           ttACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGa                                                                                       <  2:3442093/150‑1 (MQ=255)
                                                            tACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGAt                                                                                      >  2:1398027/1‑150 (MQ=255)
                                                            tACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGAt                                                                                      <  1:3162884/150‑1 (MQ=255)
                                                             aCCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATg                                                                                     <  1:2994803/150‑1 (MQ=255)
                                                               cATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGtt                                                                                   >  2:2542398/1‑150 (MQ=255)
                                                                aTGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTc                                                                                  <  1:337618/150‑1 (MQ=255)
                                                                aTGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTc                                                                                  >  1:238023/1‑150 (MQ=255)
                                                                 tGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCg                                                                                 <  2:659953/150‑1 (MQ=255)
                                                                 tGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCg                                                                                 <  2:659959/150‑1 (MQ=255)
                                                                   ctctCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGcc                                                                               <  1:1276346/150‑1 (MQ=255)
                                                                    tctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGccc                                                                              <  2:1232313/150‑1 (MQ=255)
                                                                     ctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCa                                                                             >  1:3803067/1‑150 (MQ=255)
                                                                     ctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCa                                                                             >  1:3784322/1‑150 (MQ=255)
                                                                     ctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCa                                                                             >  2:1188974/1‑150 (MQ=255)
                                                                     ctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCa                                                                             >  2:561076/1‑150 (MQ=255)
                                                                          cacTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCa                                                                        <  1:3466360/150‑1 (MQ=255)
                                                                           acTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCAt                                                                       <  1:2838027/150‑1 (MQ=255)
                                                                               cgccgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGtt                                                                   >  2:2073162/1‑150 (MQ=255)
                                                                                  cgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTAt                                                                <  1:1479721/150‑1 (MQ=255)
                                                                                  cgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTAt                                                                >  1:828459/1‑150 (MQ=255)
                                                                                     aCTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGaa                                                             <  1:661098/150‑1 (MQ=255)
                                                                                      ctctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAAc                                                            <  1:1673584/150‑1 (MQ=255)
                                                                                        ctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCa                                                          >  2:411495/1‑150 (MQ=255)
                                                                                         tATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCAt                                                         <  1:8844/150‑1 (MQ=255)
                                                                                           tttGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTg                                                       <  2:382827/150‑1 (MQ=255)
                                                                                             tGCGCTGCTGGCTTGTGCGTCTTTGATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGt                                                     <  2:2156698/150‑1 (MQ=255)
                                                                                              gcgcTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTg                                                    <  2:1562060/150‑1 (MQ=255)
                                                                                               cgcTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGa                                                   <  2:2042460/150‑1 (MQ=255)
                                                                                                  tgctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAat                                                <  2:3766703/150‑1 (MQ=255)
                                                                                                  tgctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAat                                                <  2:884512/150‑1 (MQ=255)
                                                                                                    ctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAatat                                              <  1:1908231/150‑1 (MQ=255)
                                                                                                    ctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAatat                                              <  1:3602166/150‑1 (MQ=255)
                                                                                                      ggCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCa                                            <  1:278748/150‑1 (MQ=255)
                                                                                                        cTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAgg                                          <  1:3770850/150‑1 (MQ=255)
                                                                                                         ttGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGc                                         <  2:808920/150‑1 (MQ=255)
                                                                                                             gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGAc                                     <  1:459136/150‑1 (MQ=255)
                                                                                                               gTCTTTTATCGTCCATGCCGCTGCACCAGATGAGATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACgg                                   >  2:2837119/1‑150 (MQ=255)
                                                                                                                tCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGt                                  <  2:584296/150‑1 (MQ=255)
                                                                                                                tCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGt                                  <  1:2325683/150‑1 (MQ=255)
                                                                                                                tCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGt                                  <  1:516247/150‑1 (MQ=255)
                                                                                                                  ttttATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTc                                >  1:3692747/1‑150 (MQ=255)
                                                                                                                    ttATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCgg                              >  1:1097124/1‑150 (MQ=255)
                                                                                                                     tATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGt                             >  1:1421745/1‑150 (MQ=255)
                                                                                                                     tATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGt                             >  1:2199803/1‑150 (MQ=255)
                                                                                                                     tATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGt                             >  2:1307132/1‑150 (MQ=255)
                                                                                                                     tATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGt                             >  1:2431722/1‑150 (MQ=255)
                                                                                                                      aTCGTCCATGCCGCTGCACCAGATGAAAAAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTg                            >  2:2628960/1‑150 (MQ=255)
                                                                                                                        cGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGAt                          >  2:2944138/1‑150 (MQ=255)
                                                                                                                          tCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATcc                        >  1:1237090/1‑150 (MQ=255)
                                                                                                                           ccATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATccc                       <  1:485165/150‑1 (MQ=255)
                                                                                                                              tGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtg                    <  2:1335949/150‑1 (MQ=255)
                                                                                                                                ccGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggc                  <  1:2414938/150‑1 (MQ=255)
                                                                                                                                 cGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggct                 >  2:3295617/1‑150 (MQ=255)
                                                                                                                                   cTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggctgg               <  2:2710647/150‑1 (MQ=255)
                                                                                                                                    tGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggctggc              >  2:1096547/1‑150 (MQ=255)
                                                                                                                                     gCACCAGATGAAATAACCTCCGCCTGGCCGGTGTATGTCGGGCCACTAACCCCGCACCTTTACTCGCCTACCCCGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGATATATCAGGCAGACGGTTCGTTGATCCCGTGGCTGGCa             <  2:2183026/150‑1 (MQ=255)
                                                                                                                                     gCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCa             <  2:3036490/150‑1 (MQ=255)
                                                                                                                                      cACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaa            <  1:326841/150‑1 (MQ=255)
                                                                                                                                        ccAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaaaa          >  2:1707417/1‑150 (MQ=255)
                                                                                                                                        ccAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaaaa          >  2:2789388/1‑150 (MQ=255)
                                                                                                                                             tGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCGGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTg     >  2:1953476/1‑150 (MQ=255)
                                                                                                                                              gAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTgg    >  2:3563304/1‑150 (MQ=255)
                                                                                                                                               aaaTAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGa   >  1:2608042/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGAC  >  CP009273/3606963‑3607256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑