Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,657,375 G→A P202S (CCT→TCT)  gadW ← transcriptional activator of gadA and gadBC; repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,657,3750GA99.5% 729.8 / NA 218P202S (CCT→TCT) gadWtranscriptional activator of gadA and gadBC; repressor of gadX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (117/100);  minor base T (0/1);  total (117/101)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

CAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATAC  >  CP009273/3657228‑3657520
                                                                                                                                                   |                                                                                                                                                 
cAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGaaa                                                                                                                                                 <  1:901848/150‑1 (MQ=255)
 aaTATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAt                                                                                                                                                <  2:3238169/150‑1 (MQ=255)
 aaTATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAt                                                                                                                                                <  2:3792101/150‑1 (MQ=255)
  atatTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAtt                                                                                                                                               >  2:1515434/1‑150 (MQ=255)
   tatTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAttt                                                                                                                                              >  2:3562036/1‑150 (MQ=255)
    atTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTg                                                                                                                                             >  2:3460484/1‑150 (MQ=255)
     ttCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAAAGAAATTTGa                                                                                                                                            >  1:582370/1‑150 (MQ=255)
       cAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACg                                                                                                                                          >  2:2039292/1‑150 (MQ=255)
        aaaTGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGt                                                                                                                                         >  2:176267/1‑150 (MQ=255)
          aTGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTaa                                                                                                                                       <  1:2310690/150‑1 (MQ=255)
             cGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTc                                                                                                                                    >  1:1067699/1‑150 (MQ=255)
                aaTATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAg                                                                                                                                 <  1:1209574/150‑1 (MQ=255)
                    tGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAAt                                                                                                                             >  1:3108523/1‑150 (MQ=255)
                        aGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTc                                                                                                                         >  2:3647582/1‑150 (MQ=255)
                        aGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTc                                                                                                                         <  2:3214339/150‑1 (MQ=255)
                         ggAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCt                                                                                                                        <  1:2796189/150‑1 (MQ=255)
                          gAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTg                                                                                                                       >  2:958133/1‑150 (MQ=255)
                           aaaaGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTgg                                                                                                                      <  2:1277678/150‑1 (MQ=255)
                              aGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCa                                                                                                                   >  1:3639594/1‑150 (MQ=255)
                                 tACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCg                                                                                                                >  1:641289/1‑150 (MQ=255)
                                    cTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACa                                                                                                             >  2:2556358/1‑150 (MQ=255)
                                     tGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACAt                                                                                                            >  1:1275803/1‑150 (MQ=255)
                                     tGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACAt                                                                                                            >  1:1293417/1‑150 (MQ=255)
                                        cGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCt                                                                                                         <  2:888654/150‑1 (MQ=255)
                                        cGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCt                                                                                                         <  1:2494416/150‑1 (MQ=255)
                                        cGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCt                                                                                                         <  1:1603940/150‑1 (MQ=255)
                                         gAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTg                                                                                                        >  2:1701147/1‑150 (MQ=255)
                                          aaTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTgg                                                                                                       <  2:3352146/150‑1 (MQ=255)
                                          aaTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTgg                                                                                                       <  2:2216557/150‑1 (MQ=255)
                                           aTGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGa                                                                                                      <  1:1088575/150‑1 (MQ=255)
                                            tGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAg                                                                                                     <  1:2621350/150‑1 (MQ=255)
                                            tGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAg                                                                                                     <  2:400983/150‑1 (MQ=255)
                                             gTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAgg                                                                                                    <  2:1335659/150‑1 (MQ=255)
                                              ttGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGc                                                                                                   <  2:3774374/150‑1 (MQ=255)
                                              ttGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGc                                                                                                   <  1:1093595/150‑1 (MQ=255)
                                                gcgcAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGa                                                                                                 <  1:3766785/150‑1 (MQ=255)
                                                 cgcAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAg                                                                                                <  1:1397394/150‑1 (MQ=255)
                                                     aaCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAat                                                                                            >  1:977761/1‑150 (MQ=255)
                                                     aaCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAat                                                                                            <  1:3562036/150‑1 (MQ=255)
                                                        tGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATAtt                                                                                         >  2:546397/1‑150 (MQ=255)
                                                         gATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATAttt                                                                                        <  2:1218372/150‑1 (MQ=255)
                                                          aTGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATAtttt                                                                                       >  2:2573033/1‑150 (MQ=255)
                                                            gtgGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTAc                                                                                     <  2:3197615/150‑1 (MQ=255)
                                                             tgGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACt                                                                                    <  2:116753/150‑1 (MQ=255)
                                                              ggCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTg                                                                                   >  1:3799740/1‑150 (MQ=255)
                                                              ggCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTg                                                                                   <  2:3109443/150‑1 (MQ=255)
                                                               gCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAATAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGa                                                                                  <  1:2466690/150‑1 (MQ=255)
                                                                 gTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa                                                                                >  2:619334/1‑150 (MQ=255)
                                                                  ttACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAAc                                                                               <  2:1044362/150‑1 (MQ=255)
                                                                    acacCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAg                                                                             >  2:3277676/1‑150 (MQ=255)
                                                                    acacCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAg                                                                             >  1:2837402/1‑150 (MQ=255)
                                                                     cacCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAgg                                                                            <  1:2280781/150‑1 (MQ=255)
                                                                          taataTTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTAttt                                                                       <  2:2893974/150‑1 (MQ=255)
                                                                          taataTTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTAttt                                                                       >  2:3255562/1‑150 (MQ=255)
                                                                           aataTTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTAtttt                                                                      >  2:3696025/1‑150 (MQ=255)
                                                                            attttttCGATATGTGTTTATATAGTACGATGTACTGCTATAGCCACTTTTTTCCGATATAGTATGCAGAGTATTTTGTCTTAACTCGTGTATTCGTCTGGCCTTCGACATCCTGGAGGCGTGTAATATTTTACTGTAACAGGTAtttta                                                                     <  2:3474196/144‑2 (MQ=255)
                                                                              atTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCAt                                                                   <  2:2749946/150‑1 (MQ=255)
                                                                              atTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCAt                                                                   >  1:2762387/1‑150 (MQ=255)
                                                                                 gTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCt                                                                >  2:197983/1‑150 (MQ=255)
                                                                                    gAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCa                                                             <  1:472480/150‑1 (MQ=255)
                                                                                     aaaTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCaa                                                            >  2:1063049/1‑150 (MQ=255)
                                                                                      aaTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAAc                                                           <  1:3654908/150‑1 (MQ=255)
                                                                                          tgtTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtttt                                                       >  1:1562988/1‑150 (MQ=255)
                                                                                               ataAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTg                                                  >  1:2382237/1‑150 (MQ=255)
                                                                                                 aaaGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGAt                                                >  1:3776917/1‑150 (MQ=255)
                                                                                                  aaGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGAtt                                               <  1:2872005/150‑1 (MQ=255)
                                                                                                  aaGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGAtt                                               >  1:64902/1‑150 (MQ=255)
                                                                                                   aGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTCTTTTCATCCTGCAACTTTTTTTTGATTa                                              >  2:1465862/1‑150 (MQ=255)
                                                                                                    gTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAg                                             <  1:3460484/150‑1 (MQ=255)
                                                                                                    gTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAg                                             >  2:1397140/1‑150 (MQ=255)
                                                                                                     tACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGa                                            >  2:2615006/1‑150 (MQ=255)
                                                                                                     tACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGa                                            >  1:1953401/1‑150 (MQ=255)
                                                                                                     tACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGa                                            <  1:1607767/150‑1 (MQ=255)
                                                                                                     tACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGa                                            <  1:730104/150‑1 (MQ=255)
                                                                                                      aCGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGAc                                           <  1:1813163/150‑1 (MQ=255)
                                                                                                         aTGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGActct                                        >  1:102866/1‑150 (MQ=255)
                                                                                                          tGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACtctc                                       >  2:3240929/1‑150 (MQ=255)
                                                                                                              cTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTg                                   >  1:45151/1‑150 (MQ=255)
                                                                                                              cTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTg                                   >  1:2680478/1‑150 (MQ=255)
                                                                                                              cTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTg                                   >  2:2814538/1‑150 (MQ=255)
                                                                                                                gCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGt                                 <  1:3341639/150‑1 (MQ=255)
                                                                                                                gCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGt                                 >  2:557342/1‑150 (MQ=255)
                                                                                                                  tataGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGtat                               <  2:977761/150‑1 (MQ=255)
                                                                                                                   ataGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCTCTGGtata                              >  1:2118835/1‑150 (MQ=255)
                                                                                                                     aGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACa                            >  1:3471062/1‑150 (MQ=255)
                                                                                                                              tttCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCcg                   >  2:2355020/1‑150 (MQ=255)
                                                                                                                              tttCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCcg                   >  2:2962419/1‑150 (MQ=255)
                                                                                                                                   gCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGata              >  2:2175604/1‑150 (MQ=255)
                                                                                                                                   gCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGata              >  2:1635300/1‑150 (MQ=255)
                                                                                                                                   gCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGata              >  2:857531/1‑150 (MQ=255)
                                                                                                                                    cAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGatat             >  1:516346/1‑150 (MQ=255)
                                                                                                                                    cAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGatat             >  1:2591118/1‑150 (MQ=255)
                                                                                                                                     aaTAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATc            <  1:176267/150‑1 (MQ=255)
                                                                                                                                     aaTAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATc            <  2:1140295/150‑1 (MQ=255)
                                                                                                                                      atatTATGCAGAGAAATTTGACGTAACTCGAGTAAACGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTCTACATTCTTTCCTCGATATcg           >  2:1031741/1‑150 (MQ=255)
                                                                                                                                      aTAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcg           <  1:480154/150‑1 (MQ=255)
                                                                                                                                        aGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcgcg         >  2:1571797/1‑150 (MQ=255)
                                                                                                                                          tATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCa       <  1:146169/150‑1 (MQ=255)
                                                                                                                                          tATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCa       >  1:1103941/1‑150 (MQ=255)
                                                                                                                                               agagAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATAc  >  1:2285619/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATAC  >  CP009273/3657228‑3657520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑