Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,732,549 G→T D565Y (GAT→TAT)  malS → alpha‑amylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,732,5490GT100.0% 720.0 / NA 214D565Y (GAT→TAT) malSalpha‑amylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (104/110);  total (104/110)

Only 100 of 216 total aligned reads displayed.

CGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGAT  >  CP009273/3732402‑3732695
                                                                                                                                                   |                                                                                                                                                  
     cGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCg                                                                                                                                             >  2:732967/1‑150 (MQ=255)
     cGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCg                                                                                                                                             >  1:2847214/1‑150 (MQ=255)
           cgatgcgatgATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGtt                                                                                                                                       <  1:653129/150‑1 (MQ=255)
              tgcgatgATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCg                                                                                                                                    <  1:1394390/150‑1 (MQ=255)
                   tgatCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAg                                                                                                                               >  2:1652274/1‑150 (MQ=255)
                   tgatCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAg                                                                                                                               <  1:2324053/150‑1 (MQ=255)
                    gatCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAgg                                                                                                                              <  1:1877675/150‑1 (MQ=255)
                       cAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAggggg                                                                                                                           >  1:2072532/1‑150 (MQ=255)
                        aaTTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGc                                                                                                                          <  1:2199299/150‑1 (MQ=255)
                        aaTTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGc                                                                                                                          <  1:290964/150‑1 (MQ=255)
                         aTTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTCTACCCGCCTGTTCCGTGAAGGGGGCg                                                                                                                         >  1:2961659/1‑150 (MQ=255)
                         aTTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCg                                                                                                                         <  2:1826814/150‑1 (MQ=255)
                             cGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACaa                                                                                                                     >  1:3319724/1‑150 (MQ=255)
                             cGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACaa                                                                                                                     >  2:631258/1‑150 (MQ=255)
                               aTTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAag                                                                                                                   <  1:2560424/150‑1 (MQ=255)
                                ttATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAagc                                                                                                                  >  1:2229612/1‑150 (MQ=255)
                                ttATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAagc                                                                                                                  <  1:3385597/150‑1 (MQ=255)
                                 tATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAagca                                                                                                                 <  1:2132951/150‑1 (MQ=255)
                                 tATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGGCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAagca                                                                                                                 >  2:2499797/1‑150 (MQ=255)
                                  aTCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAagcag                                                                                                                <  1:701414/150‑1 (MQ=255)
                                    cAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAgcagca                                                                                                              >  2:2850928/1‑150 (MQ=255)
                                      ggAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCaga                                                                                                            >  2:3774416/1‑150 (MQ=255)
                                        aGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGt                                                                                                          >  2:1910645/1‑150 (MQ=255)
                                        aGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGAGAAGGGGGCGACAAAGCAGCAGAGt                                                                                                          >  1:579030/1‑150 (MQ=255)
                                         gCAGGCGGCGATAGCAGTCGACTGTCTGGCGCAGCTGGAGACGACCTGGCAGCAAATGGCGGAGACAGTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATTCCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGTGtt                                                                                                         <  2:2177248/150‑1 (MQ=255)
                                           aGGCGGCGAAATCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGttat                                                                                                       <  1:1001587/150‑1 (MQ=255)
                                           aGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGttat                                                                                                       <  2:2246359/150‑1 (MQ=255)
                                           aGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGttat                                                                                                       >  1:60399/1‑150 (MQ=255)
                                           aGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGttat                                                                                                       >  2:1431118/1‑150 (MQ=255)
                                           aGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGttat                                                                                                       <  2:347394/150‑1 (MQ=255)
                                            ggcggcGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGttatt                                                                                                      >  2:2314946/1‑150 (MQ=255)
                                             gcggcgAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGttatta                                                                                                     <  1:2527397/150‑1 (MQ=255)
                                               ggcgAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACt                                                                                                   >  1:319905/1‑150 (MQ=255)
                                               ggcgAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACt                                                                                                   >  1:3512894/1‑150 (MQ=255)
                                                 cgAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTAt                                                                                                 >  1:3709650/1‑150 (MQ=255)
                                                 cgAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTAt                                                                                                 <  2:3548691/150‑1 (MQ=255)
                                                  gAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTAtt                                                                                                >  2:1361169/1‑150 (MQ=255)
                                                   aaaGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTa                                                                                               >  2:2311107/1‑150 (MQ=255)
                                                   aaaGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTa                                                                                               >  2:68720/1‑150 (MQ=255)
                                                   aaaGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTa                                                                                               >  2:1979456/1‑150 (MQ=255)
                                                    aaGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAg                                                                                              <  2:602437/150‑1 (MQ=255)
                                                    aaGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAg                                                                                              <  2:3430833/150‑1 (MQ=255)
                                                       cAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAgcgc                                                                                           >  1:2565627/1‑150 (MQ=255)
                                                          tCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAg                                                                                        >  2:1588868/1‑150 (MQ=255)
                                                          tCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAg                                                                                        <  1:860385/150‑1 (MQ=255)
                                                            gACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGgc                                                                                      <  1:1740744/150‑1 (MQ=255)
                                                               tGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGcgcg                                                                                   <  1:447848/150‑1 (MQ=255)
                                                               tGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGcgcg                                                                                   <  1:2354402/150‑1 (MQ=255)
                                                                  cTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTa                                                                                <  1:270190/150‑1 (MQ=255)
                                                                         aGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGAGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCt                                                                         >  1:3525336/1‑150 (MQ=255)
                                                                          gATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCtt                                                                        >  2:2579275/1‑150 (MQ=255)
                                                                          gATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCtt                                                                        <  1:392519/150‑1 (MQ=255)
                                                                          gATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCtt                                                                        <  1:1251913/150‑1 (MQ=255)
                                                                           aTGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCttt                                                                       <  2:769330/150‑1 (MQ=255)
                                                                           aTGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCttt                                                                       >  1:3418320/1‑150 (MQ=255)
                                                                            tGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCtttt                                                                      >  2:3326304/1‑150 (MQ=255)
                                                                            tGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCtttt                                                                      >  2:68015/1‑150 (MQ=255)
                                                                               aTACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATg                                                                   <  2:2402912/150‑1 (MQ=255)
                                                                               aTACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATg                                                                   <  2:1503400/150‑1 (MQ=255)
                                                                                    aCCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGtgat                                                              >  2:3062820/1‑150 (MQ=255)
                                                                                     ccTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGtgatg                                                             <  2:1843311/150‑1 (MQ=255)
                                                                                     ccTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGtgatg                                                             >  1:1614425/1‑150 (MQ=255)
                                                                                      cTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGtgatga                                                            >  1:3564745/1‑150 (MQ=255)
                                                                                           agcaAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCt                                                       <  2:1191455/150‑1 (MQ=255)
                                                                                               aaTGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTcgcg                                                   <  1:732967/150‑1 (MQ=255)
                                                                                               aaTGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTcgcg                                                   >  1:2763029/1‑150 (MQ=255)
                                                                                                   gCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCg                                               <  2:1745923/150‑1 (MQ=255)
                                                                                                     ggAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGtt                                             >  1:1290620/1‑150 (MQ=255)
                                                                                                     ggAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGtt                                             <  2:19658/150‑1 (MQ=255)
                                                                                                     ggAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGtt                                             >  1:2036506/1‑150 (MQ=255)
                                                                                                      gagaAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTc                                            <  1:2492089/150‑1 (MQ=255)
                                                                                                       agaAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCg                                           >  1:3076086/1‑150 (MQ=255)
                                                                                                          aaTTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTc                                        >  2:331299/1‑150 (MQ=255)
                                                                                                           aTTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTcc                                       >  1:1634381/1‑150 (MQ=255)
                                                                                                            ttGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCt                                      <  2:2275857/150‑1 (MQ=255)
                                                                                                             tGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTa                                     >  2:107323/1‑150 (MQ=255)
                                                                                                              gCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTAc                                    <  1:3722533/150‑1 (MQ=255)
                                                                                                               cAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACa                                   >  2:3792815/1‑150 (MQ=255)
                                                                                                               cAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACa                                   <  1:3047937/150‑1 (MQ=255)
                                                                                                                 gggTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAgg                                 <  2:454454/150‑1 (MQ=255)
                                                                                                                 gggTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAgg                                 <  2:2085509/150‑1 (MQ=255)
                                                                                                                 gggTTTCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAgg                                 <  2:3286603/150‑1 (MQ=255)
                                                                                                                   gTTTCAACGTGTTGAGCTACCTCTCGTCGCATTAGACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGtt                               <  2:3594962/150‑1 (MQ=255)
                                                                                                                      tCAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTg                            <  1:1565510/150‑1 (MQ=255)
                                                                                                                       cAACGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGa                           <  1:1771252/150‑1 (MQ=255)
                                                                                                                        aaCGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGAt                          >  1:1456995/1‑150 (MQ=255)
                                                                                                                        aaCGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGAt                          >  1:2049300/1‑150 (MQ=255)
                                                                                                                        aaCGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGAt                          >  1:1927076/1‑150 (MQ=255)
                                                                                                                        aaCGTGTTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGAt                          <  2:3148188/150‑1 (MQ=255)
                                                                                                                             gtTGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCt                     <  1:1979112/150‑1 (MQ=255)
                                                                                                                              ttGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTg                    <  1:990041/150‑1 (MQ=255)
                                                                                                                              ttGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTg                    >  1:2392867/1‑150 (MQ=255)
                                                                                                                               tGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGc                   <  1:1521202/150‑1 (MQ=255)
                                                                                                                               tGAGCTACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGc                   <  1:1089895/150‑1 (MQ=255)
                                                                                                                                    tACCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGt              >  1:2398954/1‑150 (MQ=255)
                                                                                                                                     aCCTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTa             >  1:3188245/1‑150 (MQ=255)
                                                                                                                                      ccTCTCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTac            <  2:214269/150‑1 (MQ=255)
                                                                                                                                       ctctCGTCGCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTaca           <  2:249126/150‑1 (MQ=255)
                                                                                                                                              cgCATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCgg    >  2:2805977/1‑150 (MQ=255)
                                                                                                                                                cATTATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGat  <  2:3613600/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAGCAGTCGACTGTCTGGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGAT  >  CP009273/3732402‑3732695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑