Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,767,410 G→T V590V (GTG→GTT)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,767,4100GT100.0% 653.4 / NA 195V590V (GTG→GTT) mtlAfused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (109/86);  total (109/86)

Only 100 of 195 total aligned reads displayed.

ACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATC  >  CP009273/3767264‑3767556
                                                                                                                                                  |                                                                                                                                                  
   ccGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGt                                                                                                                                              <  1:2779479/150‑1 (MQ=255)
    cGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATGGCCCGTCTGGTTATTGGTa                                                                                                                                             <  2:1759352/150‑1 (MQ=255)
      aCTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTAtt                                                                                                                                           <  2:2022210/150‑1 (MQ=255)
        ttATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgc                                                                                                                                         >  2:1688116/1‑150 (MQ=255)
        ttATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgc                                                                                                                                         <  2:3799264/150‑1 (MQ=255)
         tagcTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgct                                                                                                                                        >  1:1081831/4‑150 (MQ=255)
           tCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgctgc                                                                                                                                      <  1:624622/150‑1 (MQ=255)
            cTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGTAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgctgca                                                                                                                                     <  1:1626242/150‑2 (MQ=255)
            cTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGcc                                                                                                                                     <  1:1549384/150‑1 (MQ=255)
             tGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGccc                                                                                                                                    <  2:63340/150‑1 (MQ=255)
                gTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTa                                                                                                                                 >  1:2891217/1‑150 (MQ=255)
                gTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTa                                                                                                                                 <  2:2254136/150‑1 (MQ=255)
                gTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTa                                                                                                                                 <  2:2457482/150‑1 (MQ=255)
                    gTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaacaa                                                                                                                             <  2:399295/150‑1 (MQ=255)
                     tCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaacaac                                                                                                                            >  2:2927925/1‑150 (MQ=255)
                      cTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACg                                                                                                                           <  2:3598509/150‑1 (MQ=255)
                      cTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACg                                                                                                                           <  1:169851/150‑1 (MQ=255)
                        aTCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAg                                                                                                                         >  1:415729/1‑150 (MQ=255)
                        aTCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAg                                                                                                                         <  1:2145956/150‑1 (MQ=255)
                        aTCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAg                                                                                                                         >  1:1754533/1‑150 (MQ=255)
                               tGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTc                                                                                                                  >  1:2587252/1‑150 (MQ=255)
                                gCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCa                                                                                                                 <  1:1041629/150‑1 (MQ=255)
                                   acacGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGt                                                                                                              >  1:2068963/1‑150 (MQ=255)
                                   acacGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGt                                                                                                              >  1:2069210/1‑150 (MQ=255)
                                          acggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCAcc                                                                                                       <  2:3181780/150‑1 (MQ=255)
                                            ggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAg                                                                                                     >  2:134993/1‑150 (MQ=255)
                                            ggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAg                                                                                                     >  2:135133/1‑150 (MQ=255)
                                               tGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCt                                                                                                  >  1:1109787/1‑150 (MQ=255)
                                                     gAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCaa                                                                                            >  1:3309838/1‑150 (MQ=255)
                                                       aaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATGGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATg                                                                                          <  1:364167/150‑1 (MQ=255)
                                                         gATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                        >  1:1024693/1‑150 (MQ=255)
                                                         gATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                        >  1:414604/1‑150 (MQ=255)
                                                         gATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                        >  1:1024778/1‑150 (MQ=255)
                                                           tCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACt                                                                                      >  1:1314163/1‑150 (MQ=255)
                                                            cgcgTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTg                                                                                     >  2:774146/1‑150 (MQ=255)
                                                             gcgTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTgg                                                                                    <  1:1224645/150‑1 (MQ=255)
                                                               gTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgat                                                                                  >  2:532777/1‑150 (MQ=255)
                                                                tACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatg                                                                                 <  1:857226/150‑1 (MQ=255)
                                                                tACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatg                                                                                 >  2:2142588/1‑150 (MQ=255)
                                                                    gAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGAtgatga                                                                             >  1:2404752/1‑150 (MQ=255)
                                                                      aaaCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                           >  2:2034234/1‑150 (MQ=255)
                                                                       aaCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTc                                                                          >  2:1308829/1‑150 (MQ=255)
                                                                       aaCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTc                                                                          >  1:148080/1‑150 (MQ=255)
                                                                         cGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCg                                                                        <  1:1196268/150‑1 (MQ=255)
                                                                          gggCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGt                                                                       >  1:1682785/1‑150 (MQ=255)
                                                                              gtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                   >  2:2670265/1‑150 (MQ=255)
                                                                              gtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                   >  2:1055355/1‑150 (MQ=255)
                                                                              gtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                   >  2:2956438/1‑150 (MQ=255)
                                                                              gtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                   <  1:90609/150‑1 (MQ=255)
                                                                              gtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                   <  1:2386251/150‑1 (MQ=255)
                                                                              gtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                   <  1:1190145/150‑1 (MQ=255)
                                                                              gtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                   >  1:3187548/1‑150 (MQ=255)
                                                                              gtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                   >  1:3187577/1‑150 (MQ=255)
                                                                                  tgtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGc                                                               >  2:369349/1‑150 (MQ=255)
                                                                                  tgtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGc                                                               >  2:1338393/1‑150 (MQ=255)
                                                                                   gtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCg                                                              <  2:491778/150‑1 (MQ=255)
                                                                                   gtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCg                                                              >  1:3391362/1‑150 (MQ=255)
                                                                                    ttCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGt                                                             <  1:933809/150‑1 (MQ=255)
                                                                                     tCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTc                                                            <  1:211936/150‑1 (MQ=255)
                                                                                      cTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCt                                                           <  2:886570/150‑1 (MQ=255)
                                                                                        gCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTgg                                                         >  1:2332953/1‑150 (MQ=255)
                                                                                           aGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGcac                                                      <  2:1523321/150‑1 (MQ=255)
                                                                                              aCCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGcacaca                                                   <  1:3676841/150‑1 (MQ=255)
                                                                                                ccGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAcacc                                                 <  1:146304/150‑1 (MQ=255)
                                                                                                   gAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACaccacc                                              >  2:2572319/1‑150 (MQ=255)
                                                                                                     aGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAg                                            <  1:3175127/150‑1 (MQ=255)
                                                                                                       gCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCg                                          <  1:129849/150‑1 (MQ=255)
                                                                                                         gTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTg                                        <  2:3455355/150‑1 (MQ=255)
                                                                                                            cgcTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGAt                                     >  2:3302824/1‑150 (MQ=255)
                                                                                                             gcTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATg                                    <  2:2069210/150‑1 (MQ=255)
                                                                                                              cTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGa                                   <  1:2308746/150‑1 (MQ=255)
                                                                                                                tCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAg                                 >  1:3079051/1‑150 (MQ=255)
                                                                                                                 cGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGt                                <  1:3717837/150‑1 (MQ=255)
                                                                                                                     gaagaagaaGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCCCCAGCGTGGATGAAGTGCTg                            >  1:3533302/1‑150 (MQ=255)
                                                                                                                      aagaagaagATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTgg                           >  1:2012265/1‑150 (MQ=255)
                                                                                                                       agaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGa                          >  2:723259/1‑150 (MQ=255)
                                                                                                                        gaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGac                         >  2:1091213/1‑149 (MQ=255)
                                                                                                                         aagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAc                        <  1:610960/150‑1 (MQ=255)
                                                                                                                          agaagaTGACATTGCCCGCCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAct                       <  2:185941/150‑1 (MQ=255)
                                                                                                                            aagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgc                     >  2:2478126/1‑150 (MQ=255)
                                                                                                                            aagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgc                     >  2:2573841/1‑150 (MQ=255)
                                                                                                                              gaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgctg                   <  2:2776197/150‑1 (MQ=255)
                                                                                                                              gaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgctg                   >  1:2716344/1‑150 (MQ=255)
                                                                                                                               aTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTgg                  >  2:545808/1‑150 (MQ=255)
                                                                                                                               aTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTgg                  >  2:1266134/1‑150 (MQ=255)
                                                                                                                               aTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTgg                  >  2:2409761/1‑150 (MQ=255)
                                                                                                                                tGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGc                 >  1:2481531/1‑150 (MQ=255)
                                                                                                                                tGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGc                 >  2:1361103/1‑150 (MQ=255)
                                                                                                                                 gACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCa                >  1:97822/1‑150 (MQ=255)
                                                                                                                                  aCATTGCCAGTCTTGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTTGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCATCGTGGATGAAGTGCTGGAACTGCTGGCat               <  2:1919687/150‑2 (MQ=255)
                                                                                                                                   cATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAgg              >  2:2844443/1‑150 (MQ=255)
                                                                                                                                    aTTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt             >  2:978013/1‑150 (MQ=255)
                                                                                                                                    aTTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt             >  1:186990/1‑150 (MQ=255)
                                                                                                                                    aTTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt             >  2:3582268/1‑150 (MQ=255)
                                                                                                                                       gCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgt          >  1:722453/1‑150 (MQ=255)
                                                                                                                                        cccGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgta         <  1:1897148/150‑1 (MQ=255)
                                                                                                                                            tCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaagta     >  2:2649853/1‑150 (MQ=255)
                                                                                                                                            tCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaagta     <  2:24758/150‑1 (MQ=255)
                                                                                                                                               ggTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTaatc  <  1:2927925/150‑1 (MQ=255)
                                                                                                                                               ggTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTaatc  >  2:1122793/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATC  >  CP009273/3767264‑3767556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑