BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,825,702	C→A	K732N (AAG→AAT)	yicI ←	putative alpha‑glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,825,702	0	C	A	100.0%	634.9 / NA	189	K732N (AAG→AAT)	yicI	putative alpha‑glucosidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (97/92); total (97/92)

Only 100 of 189 total aligned reads displayed.

TACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTC  >  CP009273/3825557‑3825848

tACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcg                                                                                                                                                >  2:1409597/1‑150 (MQ=255)

tACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcg                                                                                                                                                <  2:417646/150‑1 (MQ=255)

  cccGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcc                                                                                                                                              >  2:3315663/1‑150 (MQ=255)

    cGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcctc                                                                                                                                            <  2:3509125/150‑1 (MQ=255)

     ggCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATtcgcctcg                                                                                                                                           >  1:824186/1‑150 (MQ=255)

        aTTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGccc                                                                                                                                        <  1:1508670/150‑1 (MQ=255)

          tCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGc                                                                                                                                      >  2:2498215/1‑150 (MQ=255)

               cAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAg                                                                                                                                 <  2:2742115/150‑1 (MQ=255)

                aaTAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGt                                                                                                                                >  1:2061398/1‑150 (MQ=255)

                    gATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTcaca                                                                                                                            >  2:366170/1‑150 (MQ=255)

                    gATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTcaca                                                                                                                            <  1:99328/150‑1 (MQ=255)

                    gATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTcaca                                                                                                                            >  2:2696447/1‑150 (MQ=255)

                     aTTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAg                                                                                                                           >  1:474914/1‑150 (MQ=255)

                       tACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTa                                                                                                                         <  1:2925329/150‑1 (MQ=255)

                       tACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTa                                                                                                                         <  1:2055501/150‑1 (MQ=255)

                          aaCGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATc                                                                                                                      <  1:2104002/150‑1 (MQ=255)

                          aaCGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATc                                                                                                                      <  2:759834/150‑1 (MQ=255)

                           aCGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCg                                                                                                                     <  2:3579805/150‑1 (MQ=255)

                           aCGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCg                                                                                                                     <  2:3644314/150‑1 (MQ=255)

                            cGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCgt                                                                                                                    >  1:1185335/1‑150 (MQ=255)

                               aaTTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGtt                                                                                                                 <  1:3130773/150‑1 (MQ=255)

                                   gTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCa                                                                                                             <  1:354645/150‑1 (MQ=255)

                                    tCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAg                                                                                                            >  2:2409517/1‑150 (MQ=255)

                                    tCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAg                                                                                                            >  1:2526406/1‑150 (MQ=255)

                                     cAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGt                                                                                                           >  1:2341310/1‑150 (MQ=255)

                                       gcgcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTAc                                                                                                         <  1:1970583/150‑1 (MQ=255)

                                       gcgcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTAc                                                                                                         <  1:2511364/150‑1 (MQ=255)

                                         gcATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGt                                                                                                       >  1:109437/1‑150 (MQ=255)

                                           aTTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgc                                                                                                     >  1:1402708/1‑150 (MQ=255)

                                           aTTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgc                                                                                                     <  2:1449169/150‑1 (MQ=255)

                                            ttCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgct                                                                                                    <  2:1185335/150‑1 (MQ=255)

                                                  tGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCtttt                                                                                              <  2:2978008/150‑1 (MQ=255)

                                                  tGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCtttt                                                                                              <  1:2756921/150‑1 (MQ=255)

                                                  tGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCtttt                                                                                              <  2:3340092/150‑1 (MQ=255)

                                                    aGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaa                                                                                            <  2:711735/150‑1 (MQ=255)

                                                      gCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaag                                                                                          >  2:1131835/1‑150 (MQ=255)

                                                      gCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaag                                                                                          >  1:345840/1‑150 (MQ=255)

                                                       cTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCTGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagt                                                                                         <  2:1983341/150‑1 (MQ=255)

                                                       cTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagt                                                                                         <  2:2277076/150‑1 (MQ=255)

                                                        ttCACCACCAGCCCCTGCTCTCTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagta                                                                                        <  1:3389457/150‑1 (MQ=255)

                                                        ttCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagta                                                                                        >  2:1671263/1‑150 (MQ=255)

                                                        ttCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagta                                                                                        <  1:170145/150‑1 (MQ=255)

                                                        ttCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagta                                                                                        >  1:1209932/1‑150 (MQ=255)

                                                          caccacCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagtaaa                                                                                      >  2:3799717/1‑150 (MQ=255)

                                                               ccaGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCTCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAATACGTGCTGCTTTTAAAGTAAAGATCa                                                                                 >  1:74600/1‑150 (MQ=255)

                                                               ccaGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCa                                                                                 <  1:767324/150‑1 (MQ=255)

                                                                 aGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAcc                                                                               <  2:824186/150‑1 (MQ=255)

                                                                    cccTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCGGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGAt                                                                            <  1:2665255/150‑1 (MQ=255)

                                                                    cccTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGAt                                                                            <  1:1496224/150‑1 (MQ=255)

                                                                     ccTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTGTCACAACCGTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGANc                                                                           <  1:3320967/150‑1 (MQ=255)

                                                                       tGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCg                                                                         <  1:1376534/150‑1 (MQ=255)

                                                                          tCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTca                                                                      <  2:801113/150‑1 (MQ=255)

                                                                           cACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTcag                                                                     <  1:2542753/150‑1 (MQ=255)

                                                                               ttCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCagcagc                                                                 >  2:32033/1‑150 (MQ=255)

                                                                                 cAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCgg                                                               <  2:2292774/150‑1 (MQ=255)

                                                                                  aGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCggg                                                              >  1:2240419/1‑150 (MQ=255)

                                                                                  aGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCggg                                                              >  2:3761228/1‑150 (MQ=255)

                                                                                     cTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCAc                                                           >  1:2957032/1‑150 (MQ=255)

                                                                                      tGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACt                                                          <  2:3771397/150‑1 (MQ=255)

                                                                                         gAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTca                                                       <  1:2849432/150‑1 (MQ=255)

                                                                                         gAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTca                                                       >  2:1950824/1‑150 (MQ=255)

                                                                                         gAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTca                                                       <  1:2155859/150‑1 (MQ=255)

                                                                                          aaCCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTcac                                                      <  2:284871/150‑1 (MQ=255)

                                                                                           aCCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTcaca                                                     >  2:1401720/1‑150 (MQ=255)

                                                                                            ccGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAg                                                    <  1:1260751/150‑1 (MQ=255)

                                                                                            ccGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAg                                                    <  2:213253/150‑1 (MQ=255)

                                                                                               tCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACg                                                 >  1:506478/1‑150 (MQ=255)

                                                                                                  tGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCt                                              <  1:1765997/150‑1 (MQ=255)

                                                                                                     aGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCa                                           <  1:174032/150‑1 (MQ=255)

                                                                                                        ccATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGc                                        >  1:9157/1‑150 (MQ=255)

                                                                                                           tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCg                                     >  1:2431339/1‑150 (MQ=255)

                                                                                                           tttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCg                                     <  1:1359954/150‑1 (MQ=255)

                                                                                                            ttACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGt                                    >  2:3540873/1‑150 (MQ=255)

                                                                                                               cTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCtt                                 >  2:2220489/1‑150 (MQ=255)

                                                                                                               cTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCtt                                 <  1:2409517/150‑1 (MQ=255)

                                                                                                               cTTTCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCtt                                 >  1:2582773/1‑150 (MQ=255)

                                                                                                                  tCACAACATTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCa                              >  2:483602/1‑150 (MQ=255)

                                                                                                                    acaacaTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGa                            >  2:1506655/1‑150 (MQ=255)

                                                                                                                     caacaTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAt                           >  1:3195120/1‑150 (MQ=255)

                                                                                                                      aacaTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAtt                          <  2:907063/150‑1 (MQ=255)

                                                                                                                      aacaTTGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGAtt                          >  1:629170/1‑150 (MQ=255)

                                                                                                                           tGCGCAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAaga                     >  2:2777426/1‑150 (MQ=255)

                                                                                                                            gcgcAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAagag                    <  2:2061398/150‑1 (MQ=255)

                                                                                                                            gcgcAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAagag                    <  2:2061390/150‑1 (MQ=255)

                                                                                                                             cgcAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAgg                   >  1:2264976/1‑150 (MQ=255)

                                                                                                                              gcAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGt                  <  1:1278548/150‑1 (MQ=255)

                                                                                                                              gcAGGCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGt                  <  2:2642888/150‑1 (MQ=255)

                                                                                                                                 ggCACAGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGa               <  2:1235757/150‑1 (MQ=255)

                                                                                                                                    acaGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATg            >  2:3174783/1‑150 (MQ=255)

                                                                                                                                      aGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGcc          <  1:661224/150‑1 (MQ=255)

                                                                                                                                      aGTGTCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGcc          >  2:1929936/1‑150 (MQ=255)

                                                                                                                                       gtgtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCg         <  1:1004393/150‑1 (MQ=255)

                                                                                                                                       gtgtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCg         <  1:1004165/150‑1 (MQ=255)

                                                                                                                                         gtCCAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTg       >  1:46873/1‑150 (MQ=255)

                                                                                                                                           ccAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGcc     >  2:615180/1‑150 (MQ=255)

                                                                                                                                           ccAGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGcc     >  2:2730829/1‑150 (MQ=255)

                                                                                                                                             aGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCtt   >  2:2564493/1‑150 (MQ=255)

                                                                                                                                             aGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCtt   <  1:35916/150‑1 (MQ=255)

                                                                                                                                             aGTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCtt   >  1:1370691/1‑150 (MQ=255)

                                                                                                                                              gTTATTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTc  <  2:345840/150‑1 (MQ=255)

TACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTC  >  CP009273/3825557‑3825848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`