Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 569,705 G→A 5.9% R98R (AGG→AGA)  quuD → DLP12 prophage; putative antitermination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273569,7050GA 5.9% 1319.7 / 44.6 371R98R (AGG→AGA) quuDDLP12 prophage; putative antitermination protein
Reads supporting (aligned to +/- strand):  ref base G (168/179);  new base A (10/12);  total (178/193)
Fisher's exact test for biased strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 378 total aligned reads displayed.

GACGCGATGATCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATA  >  CP009273/569556‑569854
                                                                                                                                                     |                                                                                                                                                     
gACGCGATGATCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAgg                                                                                                                                                       <  1:2942751/150‑1 (MQ=255)
   gcgATGATCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGATTa                                                                                                                                                    <  1:2311086/150‑1 (MQ=255)
      atgatCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAg                                                                                                                                                 >  2:4810863/1‑150 (MQ=255)
      atgatCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAg                                                                                                                                                 >  1:1458717/1‑150 (MQ=255)
        gatCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGaa                                                                                                                                               >  1:4764867/1‑150 (MQ=255)
         atCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGACGGTATGACATTCATGTCAATGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGaat                                                                                                                                              >  1:2539152/1‑149 (MQ=255)
         atCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGAATTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAg                                                                                                                                              >  2:3250117/1‑150 (MQ=255)
          tCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAgg                                                                                                                                             <  2:2569781/150‑1 (MQ=255)
           cATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGc                                                                                                                                            <  2:3408160/150‑1 (MQ=255)
              ttGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGa                                                                                                                                         <  2:3995799/150‑1 (MQ=255)
                 ctggTGCATGGCCCGTCTGAAAAAGAACAACAGCTATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTATGATGGTTATATCGGGAAAAGGTTACAGAATTCTGAggg                                                                                                                                      >  2:2218792/3‑150 (MQ=255)
                 cGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAggg                                                                                                                                      >  1:4438479/1‑150 (MQ=255)
                 cGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAggg                                                                                                                                      >  1:1831411/1‑150 (MQ=255)
                  gggTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGc                                                                                                                                     >  1:5133254/1‑150 (MQ=255)
                   ggTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCa                                                                                                                                    >  2:2403581/1‑150 (MQ=255)
                      gCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATaa                                                                                                                                 <  1:3298722/150‑1 (MQ=255)
                       cATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAAt                                                                                                                                >  1:4226016/1‑150 (MQ=255)
                       cATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAAt                                                                                                                                <  2:246077/150‑1 (MQ=255)
                        aTGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAAtt                                                                                                                               <  1:2514071/150‑1 (MQ=255)
                         tGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGGAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTg                                                                                                                              <  1:3104080/150‑1 (MQ=255)
                             ccGTCTGAAAAAGAACAACAGTGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAgg                                                                                                                          <  1:1739123/150‑1 (MQ=255)
                              cGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAggg                                                                                                                         <  1:3814831/150‑1 (MQ=255)
                              cGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGATTACAGAAGGCTGAGGGCATAATTGAAggg                                                                                                                         <  1:4309534/150‑1 (MQ=255)
                               gTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGAGTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATACTTGAAGGGa                                                                                                                        >  2:4116370/1‑150 (MQ=255)
                                   gAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTATGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGtt                                                                                                                    >  2:376351/1‑150 (MQ=255)
                                    aaaaaGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTa                                                                                                                   <  1:5231521/150‑1 (MQ=255)
                                         gAACAACAGAGATTTTCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGc                                                                                                              <  2:31182/150‑1 (MQ=255)
                                            caacaGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCAtt                                                                                                           <  2:3702611/150‑1 (MQ=255)
                                            caacaGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCAtt                                                                                                           >  2:3023883/1‑150 (MQ=255)
                                            caacaGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCAtt                                                                                                           <  1:224433/150‑1 (MQ=255)
                                             aacaGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTa                                                                                                          <  2:2178900/150‑1 (MQ=255)
                                              acaGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAg                                                                                                         <  1:247246/150‑1 (MQ=255)
                                               caGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGa                                                                                                        >  2:2111725/1‑150 (MQ=255)
                                                   gATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATc                                                                                                    >  1:2692926/1‑150 (MQ=255)
                                                    aTTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATcc                                                                                                   <  1:3831906/150‑1 (MQ=255)
                                                     tttGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCg                                                                                                  <  1:2023744/150‑1 (MQ=255)
                                                      ttGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCgg                                                                                                 <  2:1770744/150‑1 (MQ=255)
                                                          aCGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTag                                                                                             >  2:2621265/1‑150 (MQ=255)
                                                            gATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTagag                                                                                           <  1:4952492/150‑1 (MQ=255)
                                                            gATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGATTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTagag                                                                                           >  2:4472390/1‑150 (MQ=255)
                                                                tattaGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATgg                                                                                       >  2:4700725/1‑150 (MQ=255)
                                                                   tagtcgATTATTATGTAGTCTGTAAGACATTCATGTCACTGGCAGGAAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGAATAATTGAAGGGATGTTAATCGCATTAGATATCCGGTTAGAGATCGata                                                                                    >  2:4973586/1‑150 (MQ=255)
                                                                    agtagATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGatat                                                                                   >  1:3940174/1‑150 (MQ=255)
                                                                    agtagATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGAGGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGatat                                                                                   <  1:902654/150‑1 (MQ=255)
                                                                      tagATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCg                                                                                 <  1:4060031/150‑1 (MQ=255)
                                                                       agATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCgt                                                                                >  1:3716708/1‑150 (MQ=255)
                                                                       agATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCgt                                                                                >  2:1659214/1‑150 (MQ=255)
                                                                        gATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCgtt                                                                               <  1:3406720/150‑1 (MQ=255)
                                                                         attattATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCgttg                                                                              <  1:640554/150‑1 (MQ=255)
                                                                           tattatGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCgttgtt                                                                            >  1:1254456/1‑150 (MQ=255)
                                                                           tattatGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCgttgtt                                                                            <  1:1751502/150‑1 (MQ=255)
                                                                            attatGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTta                                                                           <  2:5031255/150‑1 (MQ=255)
                                                                             ttatGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTtaa                                                                          <  1:2865484/150‑1 (MQ=255)
                                                                               atGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCAGGTTAGAGATGGATATCGTTGTtaata                                                                        >  2:3321694/1‑150 (MQ=255)
                                                                                  tAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAAct                                                                     >  1:198181/1‑150 (MQ=255)
                                                                                     tCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGGAAAGATTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCta                                                                  <  1:1339248/150‑1 (MQ=255)
                                                                                      cGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCtaa                                                                 >  2:880271/1‑150 (MQ=255)
                                                                                       ggTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCtaat                                                                <  1:59711/150‑1 (MQ=255)
                                                                                       ggTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCtaat                                                                >  1:3165544/1‑150 (MQ=255)
                                                                                       ggTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCtaat                                                                >  2:768893/1‑150 (MQ=255)
                                                                                         tATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCtaatta                                                              <  2:1283322/150‑1 (MQ=255)
                                                                                             aCATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAatat                                                          >  2:2803733/1‑150 (MQ=255)
                                                                                              cATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATg                                                         <  1:966239/150‑1 (MQ=255)
                                                                                                    tGTCACTGGCAGGTGAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAAtt                                                   <  1:5017640/150‑1 (MQ=255)
                                                                                                    tGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAAtt                                                   >  2:4639390/1‑150 (MQ=255)
                                                                                                            gCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTa                                           <  1:4153517/150‑1 (MQ=255)
                                                                                                              aGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTaaa                                         >  1:1077227/1‑150 (MQ=255)
                                                                                                              aGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTaaa                                         >  1:1091849/1‑150 (MQ=255)
                                                                                                              aGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTaaa                                         >  2:1031446/1‑150 (MQ=255)
                                                                                                              aGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTaaa                                         >  1:1346185/1‑150 (MQ=255)
                                                                                                              aGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTaaa                                         >  1:4637780/1‑150 (MQ=255)
                                                                                                               ggTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTaaaa                                        >  1:4030165/1‑150 (MQ=255)
                                                                                                                 tAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAat                                      <  2:3946048/150‑1 (MQ=255)
                                                                                                                  aaGCATTGCTGCTCTGATGGTTATCTCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAatt                                     >  2:2646626/1‑150 (MQ=255)
                                                                                                                      aTTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAAttatta                                 <  2:65423/150‑1 (MQ=255)
                                                                                                                      aTTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAAttatta                                 <  2:623591/150‑1 (MQ=255)
                                                                                                                      aTTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAAttatta                                 >  2:494949/1‑150 (MQ=255)
                                                                                                                      aTTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAAttatta                                 <  1:3486904/150‑1 (MQ=255)
                                                                                                                         gatgctCTTATGGTTATATCGGGAAAAGTTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTaaaa                              <  2:5240246/148‑1 (MQ=255)
                                                                                                                          ctgctCTGATGGTTATATCGGGAAATGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTaaaaa                             >  1:2089714/1‑150 (MQ=255)
                                                                                                                          ctgctCTGATGGTTATATCGGGAAAATGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTaaaaa                             <  2:2496955/150‑1 (MQ=255)
                                                                                                                          ctgctCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTaaaaa                             >  1:4728074/1‑150 (MQ=255)
                                                                                                                          ctgctCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTaaaaa                             >  1:2124674/1‑150 (MQ=255)
                                                                                                                           ttctctGATGGTTATATCGGGAAAATGTTACAGAAGGCTGAGTGCATTATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAAt                            <  2:4180029/148‑1 (MQ=255)
                                                                                                                            gctCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATg                           >  2:90336/1‑150 (MQ=255)
                                                                                                                                  aTGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGt                     >  2:3493795/1‑150 (MQ=255)
                                                                                                                                    ggTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTg                   <  1:5022188/150‑1 (MQ=255)
                                                                                                                                     gTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTga                  <  2:1458717/150‑1 (MQ=255)
                                                                                                                                      ttATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATAGTTTACTAAAAATTATTAAAAATGGGGCGTTgag                 <  2:3376147/150‑1 (MQ=255)
                                                                                                                                       tataTCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTgaga                >  2:557667/1‑150 (MQ=255)
                                                                                                                                        atatCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGAc               >  2:1421159/1‑150 (MQ=255)
                                                                                                                                        atatCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGAc               <  2:382408/150‑1 (MQ=255)
                                                                                                                                         tatCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACg              >  1:725710/1‑150 (MQ=255)
                                                                                                                                              tgAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGccccc         >  2:1909205/2‑150 (MQ=255)
                                                                                                                                               gAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATTGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCa        <  2:454639/150‑1 (MQ=255)
                                                                                                                                               gAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCa        >  2:3610204/1‑150 (MQ=255)
                                                                                                                                               gAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCa        <  1:1542395/150‑1 (MQ=255)
                                                                                                                                               gAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCa        <  1:1008423/150‑1 (MQ=255)
                                                                                                                                                aaaaGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCaa       >  2:3908707/1‑150 (MQ=255)
                                                                                                                                                 aaaGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCaaa      >  1:1382220/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACGCGATGATCATTTGCGGGTGCATGGCCCGTCTGAAAAAGAACAACAGCGATTTGCACGATTTATTAGTAGATTATTATGTAGTCGGTATGACATTCATGTCACTGGCAGGTAAGCATTGCTGCTCTGATGGTTATATCGGGAAAAGGTTACAGAAGGCTGAGGGCATAATTGAAGGGATGTTAATGGCATTAGATATCCGGTTAGAGATGGATATCGTTGTTAATAACTCTAATTAATATGCCAATTGTTTACTAAAAATTATTAAAAATGGGGCGTTGAGACGCCCCCAAAAATA  >  CP009273/569556‑569854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑