BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	583,341	T→G	65.7%	S33R (AGT→CGT)	ybcH ←	PRK09936 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	583,341	0	T	G	65.7%	482.3 / inf	328	S33R (AGT→CGT)	ybcH	PRK09936 family protein
Reads supporting (aligned to +/- strand): ref base T (45/68); new base G (106/109); total (151/177)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

CGCAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACG  >  CP009273/583192‑583488

cgcAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACt                                                                                                                                                     >  1:2624564/1‑150 (MQ=255)

  cAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTAt                                                                                                                                                   <  1:4739724/150‑1 (MQ=255)

   aGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAtc                                                                                                                                                  <  1:3048649/150‑1 (MQ=255)

    gCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTAtct                                                                                                                                                 <  1:671905/150‑1 (MQ=255)

    gCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAtct                                                                                                                                                 <  2:813680/150‑1 (MQ=255)

        cGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGt                                                                                                                                             <  1:2488197/150‑1 (MQ=255)

          gCCCGCTTAAACAATACCGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGttt                                                                                                                                           <  1:136063/150‑1 (MQ=255)

          gCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGttt                                                                                                                                           >  2:4702288/1‑150 (MQ=255)

          gCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGttt                                                                                                                                           <  1:3447445/150‑1 (MQ=255)

          gCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGttt                                                                                                                                           <  2:3318858/150‑1 (MQ=255)

           cccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGtttt                                                                                                                                          <  2:4181826/150‑1 (MQ=255)

           cccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtttt                                                                                                                                          <  1:4316868/150‑1 (MQ=255)

            ccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCGTCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtg                                                                                                                                         >  2:4606183/1‑150 (MQ=255)

               cTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTgg                                                                                                                                      <  2:3444146/150‑1 (MQ=255)

                  aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTCTCGGTAACCTGACGATCTCGGTTTTGTGGTTg                                                                                                                                   >  2:1366407/1‑150 (MQ=255)

                  aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTg                                                                                                                                   >  2:5138250/1‑150 (MQ=255)

                  aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTg                                                                                                                                   <  2:778105/150‑1 (MQ=255)

                  aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTg                                                                                                                                   >  1:1104874/1‑150 (MQ=255)

                   aaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGc                                                                                                                                  <  2:4412030/150‑1 (MQ=255)

                      aaTAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAg                                                                                                                               >  1:1560598/1‑150 (MQ=255)

                      aaTAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCAGTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAg                                                                                                                               >  1:1086853/1‑150 (MQ=255)

                              gcgcTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATAcc                                                                                                                       <  1:3589362/150‑1 (MQ=255)

                               cgcTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCt                                                                                                                      <  1:820856/150‑1 (MQ=255)

                                    ttCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCAt                                                                                                                 <  2:3128462/150‑1 (MQ=255)

                                      ctggctggTTAAATGGATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCg                                                                                                               <  2:2094621/150‑1 (MQ=255)

                                           tggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAg                                                                                                          >  1:4212654/1‑150 (MQ=255)

                                             ggTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGa                                                                                                        >  1:44713/1‑150 (MQ=255)

                                              gTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCACAGGaa                                                                                                       >  2:230012/1‑150 (MQ=255)

                                              gTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaa                                                                                                       >  1:5101117/1‑150 (MQ=255)

                                              gTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaa                                                                                                       >  1:3722790/1‑150 (MQ=255)

                                                aaaTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaaa                                                                                                     >  2:3645443/1‑150 (MQ=255)

                                                aaaTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaaa                                                                                                     >  2:3750982/1‑150 (MQ=255)

                                                aaaTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaaa                                                                                                     >  1:4377128/1‑150 (MQ=255)

                                                  aTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGTTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAg                                                                                                   <  1:1431607/150‑1 (MQ=255)

                                                    gCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTCACCTGACGCTCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAggg                                                                                                 >  2:1692300/1‑150 (MQ=255)

                                                      aTCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCt                                                                                               >  2:4896765/1‑150 (MQ=255)

                                                        cGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGa                                                                                             <  1:1929835/150‑1 (MQ=255)

                                                         gCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGAc                                                                                            <  1:100711/150‑1 (MQ=255)

                                                          ccGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGAcc                                                                                           <  1:314167/150‑1 (MQ=255)

                                                           cGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCa                                                                                          >  2:2389044/1‑150 (MQ=255)

                                                           cGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCa                                                                                          <  1:4535703/150‑1 (MQ=255)

                                                             tAACGGGTCCATTGCAAAACAAGGGTCTCGAAGCCTTGCAACCGTAACTGACTCATCAGCCCCTGCCACTGGGTAGCGGTCACCTGACGCTCTCGGGTGTGAGGTTGCCCGATCATACCTGTCAGCGAGAGGGAAACTGGGCTGccccac                                                                                        >  2:2825124/1‑147 (MQ=255)

                                                             tAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaa                                                                                        >  2:369422/1‑150 (MQ=255)

                                                             tAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaa                                                                                        >  1:3002640/1‑150 (MQ=255)

                                                               aCGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATAGCAAAGGAAAAAGGGCTGACCAAAAg                                                                                      >  2:1808337/1‑150 (MQ=255)

                                                                 gggTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCa                                                                                    >  2:2772135/1‑150 (MQ=255)

                                                                  ggTCCATTGCAAAACAAGGTTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAg                                                                                   <  2:3552198/150‑1 (MQ=255)

                                                                   gTCCATTGCAAAACAAGGGTATCGTAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACGGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGGTAATACCTTTCATCGCAAGGGCAACAGGGATGACCGAAAGCGgt                                                                                  >  2:3994587/1‑150 (MQ=255)

                                                                    tCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgtg                                                                                 <  1:741646/150‑1 (MQ=255)

                                                                     ccATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgtgt                                                                                >  2:1969211/1‑150 (MQ=255)

                                                                       aTTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCa                                                                              <  1:843638/150‑1 (MQ=255)

                                                                          gCAAAACAAGGGTATCGAAGCCTTTCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGATAAAGGGCTGACCAAAAGCAGTGTCAGCa                                                                           <  1:4937649/150‑1 (MQ=255)

                                                                           cAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCaa                                                                          >  2:2455783/1‑150 (MQ=255)

                                                                           cAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCaa                                                                          >  2:3335303/1‑150 (MQ=255)

                                                                            aaaaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAAt                                                                         >  1:151647/1‑150 (MQ=255)

                                                                                  aGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGACACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAAGACCTTTCATCGCAAATGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaaa                                                                   <  2:66443/150‑1 (MQ=255)

                                                                                   gggTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaaaa                                                                  >  1:647475/1‑150 (MQ=255)

                                                                                    ggTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAAt                                                                 <  1:2165540/150‑1 (MQ=255)

                                                                                     gTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATg                                                                <  2:4768536/150‑1 (MQ=255)

                                                                                       aTCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGaa                                                              <  2:4788021/150‑1 (MQ=255)

                                                                                        tCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAAc                                                             >  2:538277/1‑150 (MQ=255)

                                                                                        tCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAAc                                                             <  1:2931509/150‑1 (MQ=255)

                                                                                         cGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACt                                                            >  2:3406417/1‑150 (MQ=255)

                                                                                                ttGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAt                                                     >  1:4120175/1‑150 (MQ=255)

                                                                                                ttGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAt                                                     <  1:1170906/150‑1 (MQ=255)

                                                                                                 tGCAATCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGGTGCCAGTTAATACCTTTCATCGCAATGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAACATGAACTTACGCAtt                                                    >  2:1273968/1‑150 (MQ=255)

                                                                                                 tGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAtt                                                    >  1:1855821/1‑150 (MQ=255)

                                                                                                  gCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAttt                                                   <  1:2052884/150‑1 (MQ=255)

                                                                                                    aaaCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTAc                                                 >  1:2096829/1‑150 (MQ=255)

                                                                                                        gTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGt                                             >  2:2906612/1‑150 (MQ=255)

                                                                                                         tAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTg                                            <  1:853815/150‑1 (MQ=255)

                                                                                                         tAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTg                                            <  1:132335/150‑1 (MQ=255)

                                                                                                         tAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTTCCAGTg                                            >  2:1661757/1‑150 (MQ=255)

                                                                                                          aaCTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGc                                           <  2:4326248/150‑1 (MQ=255)

                                                                                                            cTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCAc                                         <  1:3416017/150‑1 (MQ=255)

                                                                                                             tGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAATGGGCTGACCAAAAGCAGTGTCAGCAATAAGTATATGAAGTTAAGCATTTACCTGTGGACt                                        >  2:2716864/1‑150 (MQ=255)

                                                                                                             tGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACt                                        <  2:4114499/150‑1 (MQ=255)

                                                                                                                cTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCa                                     <  2:5071114/150‑1 (MQ=255)

                                                                                                                cTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCa                                     <  1:3947762/150‑1 (MQ=255)

                                                                                                                 tcatcaGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCaa                                    <  1:3763414/150‑1 (MQ=255)

                                                                                                                  catcaGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTCCGAAGTTACCAGTGCACTCCAAt                                   >  2:2953503/1‑150 (MQ=255)

                                                                                                                     caGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGt                                >  1:4169798/1‑150 (MQ=255)

                                                                                                                       gCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTga                              <  1:1968094/150‑1 (MQ=255)

                                                                                                                        ccccTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGTACTCCAATGGTgag                             <  1:694115/150‑1 (MQ=255)

                                                                                                                        ccccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTgag                             >  2:5206373/1‑150 (MQ=255)

                                                                                                                          ccTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCACAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGaa                           >  2:3634742/1‑150 (MQ=255)

                                                                                                                            tGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAc                         <  2:1229290/150‑1 (MQ=255)

                                                                                                                              ccACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAcgc                       >  2:160119/1‑150 (MQ=255)

                                                                                                                               cACTGTGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCTAAGGAAAAAGGGCTGACCAAAAGCAGTTTCAGCAATACGATAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAcgcg                      <  2:1026365/150‑1 (MQ=255)

                                                                                                                               cACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAcgcg                      >  1:2251202/1‑150 (MQ=255)

                                                                                                                               cACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAcgcg                      <  1:4859451/150‑1 (MQ=255)

                                                                                                                                     gTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGttgttg                >  1:3264319/1‑150 (MQ=255)

                                                                                                                                      tATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGACCTTACGCAGTTACCAGTGCACTCCAATGGTGAGAATCGGGTGGTTgc               >  2:4987078/1‑150 (MQ=255)

                                                                                                                                       aTCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTgcg              >  1:4115009/1‑150 (MQ=255)

                                                                                                                                        tCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTgcgc             <  2:2609372/150‑1 (MQ=255)

                                                                                                                                             aaCCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTcc        >  1:2393183/1‑150 (MQ=255)

                                                                                                                                              aCCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCg       >  1:171826/1‑150 (MQ=255)

                                                                                                                                               ccTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCa                                          <  2:3089159/114‑1 (MQ=255)

                                                                                                                                                   aCTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACg  >  1:3727893/1‑150 (MQ=255)

                                                                                                                                                   aCTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACg  >  1:1110994/1‑150 (MQ=255)

CGCAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACG  >  CP009273/583192‑583488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`