BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	601,154	T→A	14.3%	L63F (TTA→TTT)	ybdF ←	DUF419 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	601,154	0	T	A	14.3%	985.0 / 129.7	328	L63F (TTA→TTT)	ybdF	DUF419 family protein
Reads supporting (aligned to +/- strand): ref base T (170/111); new base A (26/21); total (196/132)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

TTAGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGC  >  CP009273/601005‑601303

ttAGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGAttt                                                                                                                                                       <  2:1951362/150‑1 (MQ=255)

ttAGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGAttt                                                                                                                                                       <  1:2721940/150‑1 (MQ=255)

  aGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGAtttca                                                                                                                                                     >  2:4478277/1‑148 (MQ=255)

    ccAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACa                                                                                                                                                   >  2:4952385/1‑150 (MQ=255)

      aGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAgg                                                                                                                                                 <  2:698451/150‑1 (MQ=255)

       gACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAggg                                                                                                                                                >  2:4448084/1‑150 (MQ=255)

        aCCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGa                                                                                                                                               >  1:1920150/1‑150 (MQ=255)

          cATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTAGAGTTGCTGATTTATCTGGGAtt                                                                                                                                             >  2:2672294/1‑150 (MQ=255)

            tCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAtttt                                                                                                                                           >  1:100444/1‑150 (MQ=255)

            tCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGAtttt                                                                                                                                           <  2:3065033/150‑1 (MQ=255)

             cAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATAGTTGCTGATTTAACAGGGAttttt                                                                                                                                          >  1:1959692/1‑150 (MQ=255)

              aaCCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTtg                                                                                                                                         >  2:413868/1‑150 (MQ=255)

                 caccaGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACATGGATTTTTGTgg                                                                                                                                      >  2:3621599/1‑150 (MQ=255)

                 caccaGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTgg                                                                                                                                      >  2:5195595/1‑150 (MQ=255)

                    caGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATc                                                                                                                                   <  1:3395722/150‑1 (MQ=255)

                       aTTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGa                                                                                                                                <  1:8051/150‑1 (MQ=255)

                        ttCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGAc                                                                                                                               <  2:2563717/150‑1 (MQ=255)

                           cACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTc                                                                                                                            >  1:633752/1‑150 (MQ=255)

                           cACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTc                                                                                                                            >  1:3259084/1‑150 (MQ=255)

                               aaTCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGCCTTCTGAt                                                                                                                        >  2:3195483/1‑150 (MQ=255)

                               aaTCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGAt                                                                                                                        >  2:188088/1‑150 (MQ=255)

                                  cGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGa                                                                                                                     <  1:3914919/150‑1 (MQ=255)

                                   gTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGAt                                                                                                                    >  1:3729062/1‑150 (MQ=255)

                                   gTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGAt                                                                                                                    >  1:1078647/1‑150 (MQ=255)

                                    ttGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATa                                                                                                                   <  1:4458847/150‑1 (MQ=255)

                                     tGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATaa                                                                                                                  <  1:3383561/150‑1 (MQ=255)

                                      gATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATAATTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATaaa                                                                                                                 >  1:5245766/1‑150 (MQ=255)

                                       aTCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAg                                                                                                                <  2:2282071/150‑1 (MQ=255)

                                         cAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGAGAAAggg                                                                                                              >  2:465093/1‑150 (MQ=255)

                                           gATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGa                                                                                                            <  2:1885534/150‑1 (MQ=255)

                                                  aaGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCcgcg                                                                                                     >  2:876197/1‑150 (MQ=255)

                                                   aGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGt                                                                                                    <  1:2226212/150‑1 (MQ=255)

                                                   aGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGt                                                                                                    >  2:3965760/1‑150 (MQ=255)

                                                       aCGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCt                                                                                                >  1:3166627/1‑150 (MQ=255)

                                                        cGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTc                                                                                               >  1:3856212/1‑150 (MQ=255)

                                                         gCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTcc                                                                                              <  2:2040369/150‑1 (MQ=255)

                                                           ttCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCcga                                                                                            >  1:960297/1‑150 (MQ=255)

                                                             cGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCcgacg                                                                                          >  2:851363/1‑150 (MQ=255)

                                                              ggAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCcgacga                                                                                         >  2:349261/1‑150 (MQ=255)

                                                              ggAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCcgacga                                                                                         >  1:171589/1‑150 (MQ=255)

                                                               gagaTTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCgacgac                                                                                        <  1:4834892/150‑1 (MQ=255)

                                                               gagaTTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCgacgac                                                                                        >  2:1386779/1‑150 (MQ=255)

                                                                agaTTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACa                                                                                       <  2:1165184/150‑1 (MQ=255)

                                                                 gaTTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTGTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAg                                                                                      >  2:4741709/1‑150 (MQ=255)

                                                                 gaTTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAg                                                                                      >  2:4805558/1‑150 (MQ=255)

                                                                  aTTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGc                                                                                     >  2:2334697/1‑150 (MQ=255)

                                                                   tttCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCa                                                                                    <  2:1425668/150‑1 (MQ=255)

                                                                   tttCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCa                                                                                    <  1:2047009/150‑1 (MQ=255)

                                                                   tttCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCa                                                                                    <  2:2895959/150‑1 (MQ=255)

                                                                    ttcttcGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCAt                                                                                   <  2:3768611/150‑1 (MQ=255)

                                                                    ttcttcGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCAt                                                                                   >  1:3291205/1‑150 (MQ=255)

                                                                     tcttcGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGAGTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGATCCGACGACATCaaa                                                                                  >  2:5043339/1‑148 (MQ=255)

                                                                          gCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAAt                                                                             <  1:5062403/150‑1 (MQ=255)

                                                                           ccGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATc                                                                            >  2:3738369/1‑150 (MQ=255)

                                                                               gATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTgc                                                                        >  2:4273859/1‑150 (MQ=255)

                                                                                 tACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTgccg                                                                      <  1:2465596/150‑1 (MQ=255)

                                                                                 tACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTgccg                                                                      >  1:2550706/1‑150 (MQ=255)

                                                                                     cTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCaa                                                                  >  2:151357/1‑150 (MQ=255)

                                                                                     cTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATCAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCaa                                                                  >  1:3610458/1‑150 (MQ=255)

                                                                                       gAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAAtt                                                                <  2:1495039/150‑1 (MQ=255)

                                                                                                tGCTTTTTATTCATGTGATACCATGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGAGGATCGGACTTCAGATAGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAAACTTTCCGACAATATAAAAAACa                                                       >  2:3715304/1‑150 (MQ=255)

                                                                                                  cTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATc                                                     <  2:3869647/150‑1 (MQ=255)

                                                                                                     tttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTCGCTCCGACGACAGCATAAAAATCTTGACGCCAATTTTAAAAACATCGaa                                                  >  2:2842866/1‑150 (MQ=255)

                                                                                                      ttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAAc                                                 <  1:2904250/150‑1 (MQ=255)

                                                                                                      ttATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAAc                                                 <  2:4056447/150‑1 (MQ=255)

                                                                                                       tATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACt                                                >  1:704668/1‑150 (MQ=255)

                                                                                                        tttCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTc                                               <  1:447188/149‑1 (MQ=255)

                                                                                                           cATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCgg                                            >  1:4599449/1‑150 (MQ=255)

                                                                                                           cATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCgg                                            >  2:1614149/1‑150 (MQ=255)

                                                                                                              gtgATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGcc                                         >  2:5214477/1‑150 (MQ=255)

                                                                                                               tgATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCa                                        <  1:413868/150‑1 (MQ=255)

                                                                                                                 aTACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCCTACAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCaaa                                      >  2:172625/1‑150 (MQ=255)

                                                                                                                     ccTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGc                                  <  1:4707594/150‑1 (MQ=255)

                                                                                                                      cTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCACTTTTAAAAACATCGAACTCCGGGCCAAAAGGcc                                 >  1:1562207/1‑150 (MQ=255)

                                                                                                                      cTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGcn                                 <  2:1224293/150‑2 (MQ=255)

                                                                                                                      cTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGcc                                 >  2:1125413/1‑150 (MQ=255)

                                                                                                                      cTGGCTTAATGCTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGcc                                 <  2:1854959/150‑1 (MQ=255)

                                                                                                                       tGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCa                                <  2:4196167/150‑1 (MQ=255)

                                                                                                                       tGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCa                                >  2:2416165/1‑150 (MQ=255)

                                                                                                                       tGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCa                                >  1:3042793/1‑150 (MQ=255)

                                                                                                                        ggCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACAACGAACTCCGGGCCAAAAGGCCAg                               >  1:1763021/1‑150 (MQ=255)

                                                                                                                        ggCTTAATGCTTGGGTATATTGGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAg                               <  2:351681/150‑1 (MQ=255)

                                                                                                                           ttAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCaa                            >  1:4174208/1‑150 (MQ=255)

                                                                                                                            tAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCaaa                           >  1:2672370/1‑150 (MQ=255)

                                                                                                                              aTGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGc                         <  1:2451796/150‑1 (MQ=255)

                                                                                                                                 cTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCg                      >  2:4346610/1‑150 (MQ=255)

                                                                                                                                 cTTGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCg                      <  1:4952385/150‑1 (MQ=255)

                                                                                                                                  ttGGGTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGa                     <  1:3057424/150‑1 (MQ=255)

                                                                                                                                   tGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGAc                    <  1:4680091/150‑1 (MQ=255)

                                                                                                                                   tGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGAc                    <  1:2306281/150‑1 (MQ=255)

                                                                                                                                    gggTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACa                   >  1:4877092/1‑150 (MQ=255)

                                                                                                                                      gTATATTTGCTGATTAAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACaaa                 >  2:4523919/1‑150 (MQ=255)

                                                                                                                                          atTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGt             >  1:4340186/1‑150 (MQ=255)

                                                                                                                                          atTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGt             >  2:2541215/1‑150 (MQ=255)

                                                                                                                                          atTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGt             >  2:2113920/1‑150 (MQ=255)

                                                                                                                                              gCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACt         >  1:2633805/1‑150 (MQ=255)

                                                                                                                                              gCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACt         >  1:5108292/1‑150 (MQ=255)

                                                                                                                                                tGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCa       >  1:4012144/1‑150 (MQ=255)

                                                                                                                                                 gATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCaa      >  2:1841320/1‑150 (MQ=255)

                                                                                                                                                     tAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGc  >  1:1307692/1‑150 (MQ=255)

TTAGCCAGACCATCAACCACCAGATTCCACGAATCGTTGATCAGATCGCGAAGTAACGCTTCGGAGATTTCTTCGCCGGGATACACTGAAATCCAGTGCTTTTTATTCATGTGATACCCTGGCTTAATGCTTGGGTATATTTGCTGATTTAACAGGGATTTTTGTGGATCGGACTTCAGATTGATAAAGGGGACGCCGCGTAGCTCCGACGACAGCATAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGC  >  CP009273/601005‑601303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`