BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	708,018	C→A	6.7%	D81Y (GAC→TAC)	ybfF ←	acyl‑CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	708,018	0	C	A	6.7%	1022.2 / 35.6	274	D81Y (GAC→TAC)	ybfF	acyl‑CoA esterase
Reads supporting (aligned to +/- strand): ref base C (121/134); new base A (5/14); total (126/148)
Fisher's exact test for biased strand distribution p-value = 9.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

AGCCGCAAAAATCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGG  >  CP009273/707869‑708166

   cGCAAAAATCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGAt                                                                                                                                                   <  1:4471648/150‑1 (MQ=255)

   cGCAAAAATCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGAt                                                                                                                                                   <  1:5105249/150‑1 (MQ=255)

    gCAAAAATCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATc                                                                                                                                                  <  2:4457978/150‑1 (MQ=255)

    gCAAAAATCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATc                                                                                                                                                  <  1:989129/150‑1 (MQ=255)

     cAAAAATCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATct                                                                                                                                                 <  2:2916114/150‑1 (MQ=255)

     cAAAAATCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTAGATct                                                                                                                                                 <  2:3393030/150‑1 (MQ=255)

      aaaaaTCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctg                                                                                                                                                >  2:1740889/1‑150 (MQ=255)

         aaTCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATctgctg                                                                                                                                             >  1:3128065/1‑150 (MQ=255)

                  atGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCag                                                                                                                                    <  1:3032794/150‑1 (MQ=255)

                  atGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCGTTGTCGATCTGCTGTGCATCCag                                                                                                                                    >  1:3666166/1‑150 (MQ=255)

                      cGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTa                                                                                                                                >  2:1214128/1‑150 (MQ=255)

                      cGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTa                                                                                                                                >  1:3299837/1‑150 (MQ=255)

                       ggCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTAt                                                                                                                               <  2:2203248/150‑1 (MQ=255)

                        gCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATc                                                                                                                              <  2:393544/150‑1 (MQ=255)

                          gTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATcaa                                                                                                                            >  2:1020364/1‑150 (MQ=255)

                          gTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATcaa                                                                                                                            <  2:3376598/150‑1 (MQ=255)

                          gTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTAGATCTGCTGTGCATCCAGAGTATcaa                                                                                                                            >  2:1299311/1‑150 (MQ=255)

                           tACGTGAGAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATcaac                                                                                                                           <  2:5217259/150‑1 (MQ=255)

                              gTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTCTCAACAAg                                                                                                                        >  2:5082631/1‑150 (MQ=255)

                                  tAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATcc                                                                                                                    <  2:13165/150‑1 (MQ=255)

                                   aGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCt                                                                                                                   <  2:1717468/150‑1 (MQ=255)

                                        aCCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGcc                                                                                                              <  1:418534/150‑1 (MQ=255)

                                          cGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCAt                                                                                                            <  2:2414711/150‑1 (MQ=255)

                                            gcgcGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCg                                                                                                          <  1:4197412/150‑1 (MQ=255)

                                              gcgATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGcc                                                                                                        <  2:4190530/150‑1 (MQ=255)

                                                gATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCgg                                                                                                      >  1:1888607/1‑150 (MQ=255)

                                                 atatCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCggg                                                                                                     <  2:2611867/150‑1 (MQ=255)

                                                 atatCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCggg                                                                                                     >  1:904158/1‑150 (MQ=255)

                                                 atatCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTAGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCggg                                                                                                     <  1:1136336/150‑1 (MQ=255)

                                                    tcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTaa                                                                                                  >  1:4392178/1‑150 (MQ=255)

                                                    tcgatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTAGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTaa                                                                                                  <  1:2216222/150‑1 (MQ=255)

                                                      gatcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAAtt                                                                                                >  2:1225085/1‑150 (MQ=255)

                                                       atcgCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTACGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTc                                                                                               <  2:3881178/150‑1 (MQ=255)

                                                           ccaccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCAtaa                                                                                           >  1:1076082/1‑148 (MQ=255)

                                                           ccaccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTa                                                                                           <  1:2612928/150‑1 (MQ=255)

                                                           ccaccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTa                                                                                           <  1:4072771/150‑1 (MQ=255)

                                                           ccaccaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTa                                                                                           <  1:5202107/150‑1 (MQ=255)

                                                             accaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTAcc                                                                                         >  2:107384/1‑150 (MQ=255)

                                                             accaGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTAcc                                                                                         >  1:2491166/1‑150 (MQ=255)

                                                                 gTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGAt                                                                                     <  1:3006516/150‑1 (MQ=255)

                                                                   ttATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGAtct                                                                                   <  1:3059041/150‑1 (MQ=255)

                                                                         aTGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGt                                                                             <  1:394718/150‑1 (MQ=255)

                                                                          tGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTg                                                                            >  1:107909/1‑150 (MQ=255)

                                                                          tGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTg                                                                            >  1:167896/1‑150 (MQ=255)

                                                                              aTCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAg                                                                        <  2:2357888/150‑1 (MQ=255)

                                                                                 ggAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGAcc                                                                     <  1:5240293/150‑1 (MQ=255)

                                                                                 ggAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGAcc                                                                     <  2:3674488/150‑1 (MQ=255)

                                                                                  gAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCg                                                                    >  1:229662/1‑150 (MQ=255)

                                                                                  gAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCg                                                                    >  1:1480616/1‑150 (MQ=255)

                                                                                    ggCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTg                                                                  <  2:2268083/150‑1 (MQ=255)

                                                                                    ggCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTg                                                                  <  1:4982530/150‑1 (MQ=255)

                                                                                      cTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGt                                                                >  1:4716496/1‑150 (MQ=255)

                                                                                       tAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGtt                                                               <  2:4108952/150‑1 (MQ=255)

                                                                                       tAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGtt                                                               <  2:1193317/150‑1 (MQ=255)

                                                                                       tAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGtt                                                               <  1:3244456/150‑1 (MQ=255)

                                                                                       tAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGtt                                                               <  2:2803505/150‑1 (MQ=255)

                                                                                        aGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGtca                                                              <  1:1233292/150‑3 (MQ=255)

                                                                                         gTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTAc                                                             <  1:1646106/150‑1 (MQ=255)

                                                                                         gTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTAc                                                             <  1:1646028/150‑1 (MQ=255)

                                                                                         gTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTAc                                                             <  1:615500/150‑1 (MQ=255)

                                                                                             aGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCat                                                         <  2:4979255/150‑1 (MQ=255)

                                                                                              gTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCata                                                        >  2:4067824/1‑150 (MQ=255)

                                                                                               tAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCatat                                                       <  2:3668051/150‑1 (MQ=255)

                                                                                                aaGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATc                                                      >  1:846462/1‑150 (MQ=255)

                                                                                                aaGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATc                                                      <  1:4613868/150‑1 (MQ=255)

                                                                                                aaGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTAGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATc                                                      <  1:3619372/150‑1 (MQ=255)

                                                                                                 aGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCa                                                     >  2:1570091/1‑150 (MQ=255)

                                                                                                   tGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAAc                                                   >  1:781615/1‑150 (MQ=255)

                                                                                                   tGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAAc                                                   >  1:3406496/1‑150 (MQ=255)

                                                                                                    gCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGACAGACCGTGGTTACGCATATCAAcc                                                  >  2:3192604/1‑150 (MQ=255)

                                                                                                    gCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAAcc                                                  <  2:1999270/150‑1 (MQ=255)

                                                                                                        ttACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGga                                              >  2:3427710/1‑150 (MQ=255)

                                                                                                         tACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGgag                                             >  1:1201521/1‑149 (MQ=255)

                                                                                                          aCCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGgatg                                            <  2:3120472/150‑1 (MQ=255)

                                                                                                            cGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGgatgat                                          >  1:1976046/1‑150 (MQ=255)

                                                                                                            cGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGgatgat                                          <  1:4652196/150‑1 (MQ=255)

                                                                                                             gCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGata                                         <  2:908732/150‑1 (MQ=255)

                                                                                                             gCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGata                                         <  2:477202/150‑1 (MQ=255)

                                                                                                              cTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGatat                                        <  2:4553414/150‑1 (MQ=255)

                                                                                                                ttACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTAGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATtg                                      <  1:1571637/150‑1 (MQ=255)

                                                                                                                 tACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATtgt                                     >  1:4123855/1‑150 (MQ=255)

                                                                                                                  aCCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATtgtg                                    >  1:307872/1‑150 (MQ=255)

                                                                                                                  aCCGCCCAGGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATtgtg                                    >  1:3173356/1‑150 (MQ=255)

                                                                                                                   ccGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGa                                   <  2:3096071/150‑1 (MQ=255)

                                                                                                                      cccATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCg                                <  1:4882396/150‑1 (MQ=255)

                                                                                                                            gAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTAcc                          <  1:4738382/150‑1 (MQ=255)

                                                                                                                            gAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATAGTGTGATCGTTTagc                          >  2:710533/1‑148 (MQ=255)

                                                                                                                            gAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCAAATCCACCTGGATGATCTTGTGATCGTTTAcc                          >  2:1908481/1‑150 (MQ=255)

                                                                                                                              gTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAg                        <  2:3553427/150‑1 (MQ=255)

                                                                                                                              gTGACCGATAAATGTTGCTTTGTAGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAg                        <  1:4347386/150‑1 (MQ=255)

                                                                                                                              gTGACCGATAAATGTTGCTTTGTAGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAg                        <  1:1319282/150‑1 (MQ=255)

                                                                                                                                  ccGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGTAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATcg                    >  2:4270869/1‑150 (MQ=255)

                                                                                                                                       aaaTGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGc               >  1:697767/1‑150 (MQ=255)

                                                                                                                                             tGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTAc         >  2:3261472/1‑150 (MQ=255)

                                                                                                                                             tGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTAc         >  2:4961821/1‑150 (MQ=255)

                                                                                                                                              gCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACg        <  2:1768025/150‑1 (MQ=255)

                                                                                                                                              gCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACg        <  1:2691047/150‑1 (MQ=255)

                                                                                                                                                   gTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAg   >  1:1761419/1‑150 (MQ=255)

                                                                                                                                                   gTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGAGATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCacg   >  2:2061551/1‑148 (MQ=255)

                                                                                                                                                    tCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAgg  >  1:277523/1‑150 (MQ=255)

AGCCGCAAAAATCTCATCATGACGGCGTACGTGATAGTCGACCGGCGCGATATCGATCGCCACCAGTTTATCGATGCGATCGGAGGCTAGTGCAGTAAGTGCCATTACCGCTTTACCGCCCATGGAGTGACCGATAAATGTTGCTTTGTCGATCTGCTGTGCATCCAGAGTATCAACAAGATCCTGCGCCATCGCCGGGTAATTCATTACCGGATCTCTCGGTGAAAGACCGTGGTTACGCATATCAACCTGGATGATATTGTGATCGTTTACCAGATCGCGAGCCAGTACGCCAAGG  >  CP009273/707869‑708166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`