Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 876,447 G→A 69.9% D89N (GAT→AAT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273876,4470GA69.9% 445.4 / 281.4 296D89N (GAT→AAT) dacCD‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)
Reads supporting (aligned to +/- strand):  ref base G (38/51);  new base A (93/114);  total (131/165)
Fisher's exact test for biased strand distribution p-value = 7.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 299 total aligned reads displayed.

GCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATT  >  CP009273/876299‑876596
                                                                                                                                                    |                                                                                                                                                     
gcgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGa                                                                                                                                                      <  1:3107138/150‑1 (MQ=255)
 cgTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGat                                                                                                                                                     >  1:4759698/1‑150 (MQ=255)
  gTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGata                                                                                                                                                    >  1:1318562/1‑150 (MQ=255)
   tGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAatat                                                                                                                                                   <  1:1909827/150‑1 (MQ=255)
      aTGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGt                                                                                                                                                <  1:82306/150‑1 (MQ=255)
       tGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTg                                                                                                                                               >  2:383455/1‑150 (MQ=255)
         gATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGAc                                                                                                                                             >  2:1449974/1‑150 (MQ=255)
              tAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGAcggtcg                                                                                                                                        >  1:273391/1‑150 (MQ=255)
                    aTTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAg                                                                                                                                  >  1:3742806/1‑150 (MQ=255)
                     ttACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGa                                                                                                                                 <  2:2120496/150‑1 (MQ=255)
                         gCCAGCGGTAAAGTGCTGGCAGACGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTCTGTGGTGGGGCCGGCGCTTAAGGCCGATCAGATTAAACTCACCGATATGGTGACGGTCGGTACAGATGcc                                                                                                                             >  2:4887379/1‑150 (MQ=255)
                          ccAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCt                                                                                                                            <  2:1107639/150‑1 (MQ=255)
                          ccAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCt                                                                                                                            <  2:2400008/150‑1 (MQ=255)
                            aGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTgg                                                                                                                          <  1:3950719/150‑1 (MQ=255)
                            aGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTgg                                                                                                                          >  1:1782313/1‑150 (MQ=255)
                            aGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTgg                                                                                                                          >  1:4325089/1‑150 (MQ=255)
                            aGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTgg                                                                                                                          >  1:2619058/1‑150 (MQ=255)
                                gTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATCAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGa                                                                                                                      >  2:2870634/1‑150 (MQ=255)
                                     gTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGa                                                                                                                 <  1:2208394/150‑1 (MQ=255)
                                      tGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGaa                                                                                                                <  2:3878896/150‑1 (MQ=255)
                                        cTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAAt                                                                                                              <  2:4974505/150‑1 (MQ=255)
                                         tGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATc                                                                                                             >  1:358247/1‑150 (MQ=255)
                                         tGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATc                                                                                                             <  2:2077130/150‑1 (MQ=255)
                                           gCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCg                                                                                                           <  1:3362689/150‑1 (MQ=255)
                                            cAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGACATCCgg                                                                                                          >  2:4402153/1‑150 (MQ=255)
                                             aGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGc                                                                                                         >  1:773123/1‑150 (MQ=255)
                                              gAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGAGAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCa                                                                                                        >  1:249298/1‑150 (MQ=255)
                                                aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACt                                                                                                      <  1:961890/150‑1 (MQ=255)
                                                aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACt                                                                                                      <  1:3700977/150‑1 (MQ=255)
                                                aGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACt                                                                                                      >  2:4209964/1‑150 (MQ=255)
                                                        cGGATGAGTAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGtt                                                                                              <  2:2937182/150‑1 (MQ=255)
                                                        cGGATGAGAAACTTGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGtt                                                                                              <  1:1084031/150‑1 (MQ=255)
                                                        cGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGtt                                                                                              <  2:612347/150‑1 (MQ=255)
                                                            tGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATc                                                                                          >  1:4037018/1‑150 (MQ=255)
                                                             gagaAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCg                                                                                         >  2:1742457/1‑150 (MQ=255)
                                                                 aaCTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTaa                                                                                     <  1:2774578/150‑1 (MQ=255)
                                                                 aaCTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTaa                                                                                     <  1:490297/150‑1 (MQ=255)
                                                                  aCTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAAt                                                                                    <  1:4228747/150‑1 (MQ=255)
                                                                    tGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGt                                                                                  >  1:1517167/1‑150 (MQ=255)
                                                                    tGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGt                                                                                  >  2:4378480/1‑150 (MQ=255)
                                                                        tCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCt                                                                              >  2:3405731/1‑150 (MQ=255)
                                                                         cccGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTc                                                                             <  1:1429342/150‑1 (MQ=255)
                                                                          ccGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCa                                                                            <  2:1927334/150‑1 (MQ=255)
                                                                              gAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAcc                                                                        >  1:442183/1‑150 (MQ=255)
                                                                              gAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAcc                                                                        <  2:3938419/150‑1 (MQ=255)
                                                                              gAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAcc                                                                        <  2:1310964/150‑1 (MQ=255)
                                                                              gAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAAcc                                                                        >  1:2991165/1‑150 (MQ=255)
                                                                                 ccTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCggg                                                                     >  1:3186678/1‑150 (MQ=255)
                                                                                    gACTAAACTCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGa                                                                  <  2:404186/150‑1 (MQ=255)
                                                                                         aaaTCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAgg                                                             >  1:2750149/1‑150 (MQ=255)
                                                                                         aaaTCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAgg                                                             >  1:2054879/1‑150 (MQ=255)
                                                                                             cATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc                                                         >  1:430660/1‑150 (MQ=255)
                                                                                             cATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTc                                                         <  1:3766338/150‑1 (MQ=255)
                                                                                                gACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGt                                                      <  1:4271179/150‑1 (MQ=255)
                                                                                                 aCCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTg                                                     >  2:210499/1‑150 (MQ=255)
                                                                                                  ccAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTgg                                                    <  2:773656/150‑1 (MQ=255)
                                                                                                   cAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGc                                                   <  2:3260741/150‑1 (MQ=255)
                                                                                                     gCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAg                                                 <  2:4552522/150‑1 (MQ=255)
                                                                                                      cTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCTGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGa                                                <  1:2295047/150‑1 (MQ=255)
                                                                                                       tATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAATCCGGGCGATCAGGTTTCGGTGGCAGAc                                               >  2:5162118/1‑150 (MQ=255)
                                                                                                       tATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGAc                                               >  1:4456123/1‑150 (MQ=255)
                                                                                                         tgtgGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACtt                                             <  2:2798984/150‑1 (MQ=255)
                                                                                                          gtgGTTGGGCAGGCGCTTATGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTg                                            <  2:4685771/150‑1 (MQ=255)
                                                                                                          gtgGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTg                                            <  1:1402090/150‑1 (MQ=255)
                                                                                                            ggTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGaa                                          <  2:4759698/150‑1 (MQ=255)
                                                                                                             gTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAAc                                         >  2:35294/1‑150 (MQ=255)
                                                                                                               tGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAACTCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACaa                                       >  1:1942410/1‑150 (MQ=255)
                                                                                                                  gCAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAgg                                    <  1:1472101/150‑1 (MQ=255)
                                                                                                                  gCAGGCGATTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAgg                                    <  2:3452148/150‑1 (MQ=255)
                                                                                                                   cAGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgt                                   >  1:5204637/1‑150 (MQ=255)
                                                                                                                    aGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtg                                  <  1:1569178/150‑1 (MQ=255)
                                                                                                                    aGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtg                                  <  1:5074513/150‑1 (MQ=255)
                                                                                                                    aGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtg                                  >  1:5191649/1‑150 (MQ=255)
                                                                                                                    aGGCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACGTGACCAAAGgtg                                  >  2:3225209/1‑150 (MQ=255)
                                                                                                                    aGGCGCTGAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtg                                  >  1:2404136/1‑150 (MQ=255)
                                                                                                                     ggCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtgt                                 <  2:4530844/150‑1 (MQ=255)
                                                                                                                     ggCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtgt                                 <  2:461120/150‑1 (MQ=255)
                                                                                                                     ggCGCTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtgt                                 >  1:570495/1‑150 (MQ=255)
                                                                                                                     ggCGCTTAAGGCCGATAAGATTAAACTCACCAATAGGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGgtgt                                 <  2:518756/150‑1 (MQ=255)
                                                                                                                       cgcTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGa                               <  1:732010/150‑1 (MQ=255)
                                                                                                                         cTTAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGAtt                             <  1:2340453/150‑1 (MQ=255)
                                                                                                                          ttAAGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTa                            >  1:4668373/1‑150 (MQ=255)
                                                                                                                            aaGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATc                          <  1:2879585/150‑1 (MQ=255)
                                                                                                                            aaGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATc                          <  2:848354/150‑1 (MQ=255)
                                                                                                                            aaGGCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAAAAAAGGTGTGATTATc                          >  1:3681784/1‑150 (MQ=255)
                                                                                                                               gCCGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAg                       >  2:2960175/1‑150 (MQ=255)
                                                                                                                                 cGATTAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTc                     <  2:4565931/150‑1 (MQ=255)
                                                                                                                                 cGATAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTc                     <  1:4501108/150‑1 (MQ=255)
                                                                                                                                   aTAAGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCg                   <  2:3379664/150‑1 (MQ=255)
                                                                                                                                    tAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTAGAACAAAGGAGTGATTATCCAGTCCgg                  >  2:4337179/1‑150 (MQ=255)
                                                                                                                                     aaGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGt                 >  1:3228551/1‑150 (MQ=255)
                                                                                                                                     aaGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGt                 <  2:1552684/150‑1 (MQ=255)
                                                                                                                                     aaGATTAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGt                 >  1:4073451/1‑150 (MQ=255)
                                                                                                                                      aGATTCAACTCACCAATCTGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTa                <  2:3723816/150‑1 (MQ=255)
                                                                                                                                        aTTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAt              <  1:3899785/150‑1 (MQ=255)
                                                                                                                                        aTTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAAt              >  2:4140271/1‑150 (MQ=255)
                                                                                                                                          tAAACTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTACTGa            >  2:3103689/1‑150 (MQ=255)
                                                                                                                                            aaCTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACg          >  1:347178/1‑150 (MQ=255)
                                                                                                                                            aaCTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACg          >  1:629919/1‑150 (MQ=255)
                                                                                                                                              cTCACCAATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGcc        <  1:3119664/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATT  >  CP009273/876299‑876596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑