BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,121,966	C→T	6.8%	R118R (CGC→CGT)	yceH →	UPF0502 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,121,966	0	C	T	6.8%	1149.1 / 47.3	331	R118R (CGC→CGT)	yceH	UPF0502 family protein
Reads supporting (aligned to +/- strand): ref base C (161/145); new base T (3/19); total (165/166)
Fisher's exact test for biased strand distribution p-value = 5.74e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 337 total aligned reads displayed.

GCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAG  >  CP009273/1121818‑1122115

gCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCa                                                                                                                                                      <  1:3353730/150‑1 (MQ=255)

  acaGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGTAgc                                                                                                                                                    <  1:3697646/150‑1 (MQ=255)

  acaGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATGTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGACCTGCGCAgc                                                                                                                                                    >  1:3262027/1‑150 (MQ=255)

   caGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCAACACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgcc                                                                                                                                                   >  2:3163040/1‑150 (MQ=255)

     gTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgccgc                                                                                                                                                 <  1:3020614/150‑1 (MQ=255)

      tGAGCGGTTTTGGTAATCGTGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATATGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCCGGTGAACTGCGAAgccgcg                                                                                                                                                >  2:5153685/1‑150 (MQ=255)

      tGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgccgcg                                                                                                                                                >  2:200890/1‑150 (MQ=255)

        aGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgccgcgcc                                                                                                                                              <  1:4999528/150‑1 (MQ=255)

        aGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgccgcgcc                                                                                                                                              >  2:3295143/1‑150 (MQ=255)

        aGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAgccgcgcc                                                                                                                                              <  2:4431278/150‑1 (MQ=255)

           ggTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGccgcgccgcg                                                                                                                                           >  2:3643912/1‑150 (MQ=255)

            gTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCcgcgccgcgc                                                                                                                                          >  2:5041600/1‑150 (MQ=255)

               ttGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTACTCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGaa                                                                                                                                       <  2:1777754/150‑1 (MQ=255)

                 ggTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATg                                                                                                                                     >  1:884648/1‑150 (MQ=255)

                 ggTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATg                                                                                                                                     >  1:1437735/1‑150 (MQ=255)

                    aaTCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTAt                                                                                                                                  >  1:3543753/1‑150 (MQ=255)

                     aTCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATg                                                                                                                                 <  2:825294/150‑1 (MQ=255)

                      tCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGa                                                                                                                                >  2:2991747/1‑150 (MQ=255)

                      tCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGa                                                                                                                                >  2:1979960/1‑150 (MQ=255)

                       cGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGTAGCCGCGCCGCGCGAATGTATGaa                                                                                                                               <  1:3171318/150‑1 (MQ=255)

                         ggTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGCATGTATGAAtt                                                                                                                             >  2:2340428/1‑150 (MQ=255)

                            cACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAg                                                                                                                          >  1:4374595/1‑150 (MQ=255)

                             aCCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGc                                                                                                                         >  2:1887948/1‑150 (MQ=255)

                              ccAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTCTGAATTCAGCg                                                                                                                        >  2:4848363/1‑150 (MQ=255)

                              ccAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCg                                                                                                                        <  2:4224270/150‑1 (MQ=255)

                                aaaTATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGat                                                                                                                      >  2:1234473/1‑150 (MQ=255)

                                aaaTATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGat                                                                                                                      <  1:659024/150‑1 (MQ=255)

                                aaaTATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGat                                                                                                                      <  1:1326601/150‑1 (MQ=255)

                                 aaTATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGata                                                                                                                     <  2:2955620/150‑1 (MQ=255)

                                  atatGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGTAGCCGCGCCGCGCGAATGTATGAATTCAGCGatat                                                                                                                    <  2:3329107/150‑1 (MQ=255)

                                    atGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGTAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATgg                                                                                                                  >  2:4344075/1‑150 (MQ=255)

                                    atGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATgg                                                                                                                  <  1:2307817/150‑1 (MQ=255)

                                    atGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATgg                                                                                                                  <  2:4598504/150‑1 (MQ=255)

                                    atGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATgg                                                                                                                  >  1:4305530/1‑150 (MQ=255)

                                     tGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGc                                                                                                                 <  2:2462026/150‑1 (MQ=255)

                                        gCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGa                                                                                                              <  1:2554627/150‑1 (MQ=255)

                                        gCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGa                                                                                                              >  2:4739335/1‑150 (MQ=255)

                                         cAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGaa                                                                                                             <  1:942502/150‑1 (MQ=255)

                                         cAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGGTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGan                                                                                                             <  2:216649/150‑2 (MQ=255)

                                          aaCGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGTAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAg                                                                                                            <  1:1173690/150‑1 (MQ=255)

                                            cGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTg                                                                                                          >  2:2668975/1‑150 (MQ=255)

                                             gTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTgg                                                                                                         <  1:4218550/150‑1 (MQ=255)

                                             gTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTgg                                                                                                         >  1:2981501/1‑150 (MQ=255)

                                               ttttGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAg                                                                                                       >  2:1377711/1‑150 (MQ=255)

                                                 ttGTAATTCAGTATTTGGCGATCTGAAACTGCGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGAGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTc                                                                                                     <  2:2281209/150‑1 (MQ=255)

                                                 ttGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGTAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTc                                                                                                     <  1:1111293/150‑1 (MQ=255)

                                                  tGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGTAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCg                                                                                                    <  1:2616496/150‑1 (MQ=255)

                                                    tAATTCAGAAGTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGAc                                                                                                  <  2:2519777/150‑1 (MQ=255)

                                                        tCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTg                                                                                              <  2:253549/150‑1 (MQ=255)

                                                        tCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTg                                                                                              <  1:749736/150‑1 (MQ=255)

                                                        tCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACCGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTg                                                                                              >  1:2073320/1‑150 (MQ=255)

                                                          aGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTCTGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGa                                                                                            >  2:27055/1‑150 (MQ=255)

                                                          aGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGa                                                                                            >  1:4027038/1‑150 (MQ=255)

                                                            aaTTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGaac                                                                                          >  1:1831654/1‑150 (MQ=255)

                                                               ttGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGaacaac                                                                                       >  2:4148605/1‑150 (MQ=255)

                                                               ttGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGaacaac                                                                                       >  1:1519709/1‑150 (MQ=255)

                                                               ttGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGaacaac                                                                                       <  1:696967/150‑1 (MQ=255)

                                                                tGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACt                                                                                      >  2:277191/1‑150 (MQ=255)

                                                                 ggCGATCTGAAACTGAGCGCAGCGGAAGTGGAGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGTAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTg                                                                                     >  1:5221861/1‑150 (MQ=255)

                                                                    gATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCa                                                                                  <  1:2725800/150‑1 (MQ=255)

                                                                        tGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATc                                                                              >  1:2896870/1‑150 (MQ=255)

                                                                          aaaCTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATcgc                                                                            >  2:713183/1‑150 (MQ=255)

                                                                           aaCTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATcgcg                                                                           >  2:1015742/1‑150 (MQ=255)

                                                                               gAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGa                                                                       >  2:1563644/1‑150 (MQ=255)

                                                                                aGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGAt                                                                      <  2:4078062/150‑1 (MQ=255)

                                                                                   gcAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGt                                                                   >  1:2409390/1‑150 (MQ=255)

                                                                                     aGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTcc                                                                 <  1:3295371/150‑1 (MQ=255)

                                                                                      gCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCt                                                                <  2:3894229/150‑1 (MQ=255)

                                                                                      gCGGAAGTGGAGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCTGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCt                                                                <  2:1755738/150‑1 (MQ=255)

                                                                                       cGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTCTGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCtg                                                               >  2:4536532/1‑149 (MQ=255)

                                                                                        ggAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCttt                                                              <  1:4736462/150‑1 (MQ=255)

                                                                                         gAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCtttt                                                             >  1:3445129/1‑150 (MQ=255)

                                                                                           aGTGGCGTTAATCACCACGTTGTTATTTCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACAGGCAAATCGCGAAGATGGTCCTTTtgt                                                           >  1:1463512/1‑150 (MQ=255)

                                                                                           aGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTtgt                                                           <  1:1320490/150‑1 (MQ=255)

                                                                                           aGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAAGGTCTGAATTCAGCGAGATGGGGGAAGTGGAGTCGACGCTGGAACAACTGGCTAATCGCGAAGATGGTCCgtgtgt                                                           >  2:4983265/1‑150 (MQ=255)

                                                                                                cGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGc                                                      <  2:1167346/150‑1 (MQ=255)

                                                                                                  ttAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGt                                                    >  1:2969674/1‑150 (MQ=255)

                                                                                                     aTCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTg                                                 <  1:3643912/150‑1 (MQ=255)

                                                                                                     aTCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTg                                                 >  2:2238906/1‑150 (MQ=255)

                                                                                                      tCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTgg                                                <  2:3019306/150‑1 (MQ=255)

                                                                                                       caccacGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGc                                               >  2:1577153/1‑150 (MQ=255)

                                                                                                          cacGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGTAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCcg                                            <  2:2085564/150‑1 (MQ=255)

                                                                                                                gttATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAAcc                                      <  1:3866980/150‑1 (MQ=255)

                                                                                                                  tATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCgg                                    >  2:1315107/1‑150 (MQ=255)

                                                                                                                     tGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGtg                                 <  2:2877106/150‑2 (MQ=255)

                                                                                                                     tGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTa                                 <  1:836403/150‑1 (MQ=255)

                                                                                                                     tGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTa                                 <  1:1200341/150‑1 (MQ=255)

                                                                                                                      gCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTaa                                <  2:3434515/150‑1 (MQ=255)

                                                                                                                       cGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTaaa                               >  2:2353096/1‑150 (MQ=255)

                                                                                                                        gtggtgCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAAc                              <  2:2861925/150‑1 (MQ=255)

                                                                                                                                 aGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAAcc                     >  2:4686340/1‑150 (MQ=255)

                                                                                                                                 aGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAAcc                     <  1:1232176/150‑1 (MQ=255)

                                                                                                                                  gACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCg                    >  1:3738675/1‑150 (MQ=255)

                                                                                                                                    cGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCt                  <  1:1367624/150‑1 (MQ=255)

                                                                                                                                      ccAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTAc                >  1:468808/1‑150 (MQ=255)

                                                                                                                                         ggTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATg             >  1:554819/1‑150 (MQ=255)

                                                                                                                                             aaCTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATc         >  2:897890/1‑150 (MQ=255)

                                                                                                                                              aCTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCt        <  2:884648/150‑1 (MQ=255)

                                                                                                                                               cTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCtt       <  2:45722/150‑1 (MQ=255)

                                                                                                                                                tGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCttt      <  1:166310/150‑1 (MQ=255)

GCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAG  >  CP009273/1121818‑1122115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`