BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,122,159	G→T	6.7%	A183S (GCG→TCG)	yceH →	UPF0502 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,122,159	0	G	T	6.7%	1047.1 / 35.9	283	A183S (GCG→TCG)	yceH	UPF0502 family protein
Reads supporting (aligned to +/- strand): ref base G (123/140); new base T (9/11); total (132/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

TGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCA  >  CP009273/1122010‑1122308

tGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAAcg                                                                                                                                                       <  1:4944996/150‑1 (MQ=255)

tGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTGTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAAcg                                                                                                                                                       >  2:3302970/1‑150 (MQ=255)

  gAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAAcgcg                                                                                                                                                     >  2:2606672/1‑150 (MQ=255)

   aGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCGGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCgg                                                                                                                                                    <  1:5208582/150‑1 (MQ=255)

     tCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGtt                                                                                                                                                  <  2:571523/150‑1 (MQ=255)

     tCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGtt                                                                                                                                                  <  1:4536219/150‑1 (MQ=255)

       gACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGa                                                                                                                                                <  1:4860097/150‑1 (MQ=255)

       gACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGa                                                                                                                                                >  1:3042469/1‑150 (MQ=255)

       gACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGa                                                                                                                                                >  1:866814/1‑150 (MQ=255)

         cGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACg                                                                                                                                              >  1:2624353/1‑150 (MQ=255)

         cGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACg                                                                                                                                              <  1:893517/150‑1 (MQ=255)

           cTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGt                                                                                                                                            <  2:2299787/150‑1 (MQ=255)

           cTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGt                                                                                                                                            <  1:270640/150‑1 (MQ=255)

           cTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGt                                                                                                                                            <  1:270535/150‑1 (MQ=255)

            tGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTg                                                                                                                                           >  1:2397423/1‑150 (MQ=255)

               aacaacTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGAtt                                                                                                                                        >  1:1263832/1‑150 (MQ=255)

                  aacTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTAc                                                                                                                                     >  2:1430528/1‑150 (MQ=255)

                    cTGGCAACTCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAg                                                                                                                                   <  2:1624805/150‑1 (MQ=255)

                    cTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAg                                                                                                                                   >  1:2083740/1‑150 (MQ=255)

                          aaTCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCcgc                                                                                                                             >  1:5064891/1‑150 (MQ=255)

                           aTCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCcgcg                                                                                                                            <  1:4677818/150‑1 (MQ=255)

                           aTCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCcgcg                                                                                                                            >  2:1532448/1‑150 (MQ=255)

                             cgcgAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAGGCCCGCGTc                                                                                                                          >  1:952341/1‑150 (MQ=255)

                             cgcgAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTc                                                                                                                          <  1:2668975/150‑1 (MQ=255)

                             cgcgAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTc                                                                                                                          <  1:1664609/150‑1 (MQ=255)

                                     tGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCt                                                                                                                  <  1:4344075/150‑1 (MQ=255)

                                         ccTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGaa                                                                                                              <  1:897890/150‑1 (MQ=255)

                                           ttttGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAAt                                                                                                            <  2:51784/150‑1 (MQ=255)

                                            tttGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATc                                                                                                           <  1:4739335/150‑1 (MQ=255)

                                             ttGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCg                                                                                                          <  2:3543748/150‑1 (MQ=255)

                                             ttGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCg                                                                                                          <  2:1463475/150‑1 (MQ=255)

                                              tgtgGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGa                                                                                                         <  1:1203506/150‑1 (MQ=255)

                                               gtggtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGaa                                                                                                        <  2:2766429/150‑1 (MQ=255)

                                               gtggtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGaa                                                                                                        <  1:2878884/150‑1 (MQ=255)

                                                tggtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAg                                                                                                       >  2:1719359/1‑150 (MQ=255)

                                                 ggtgCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGt                                                                                                      >  2:2409925/1‑150 (MQ=255)

                                                     cGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCa                                                                                                  <  2:2462254/150‑1 (MQ=255)

                                                      gTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAg                                                                                                 >  2:2326785/1‑150 (MQ=255)

                                                       tCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGa                                                                                                <  1:1015704/150‑1 (MQ=255)

                                                        cTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGaa                                                                                               >  2:3177029/1‑150 (MQ=255)

                                                                cgAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACa                                                                                       >  1:3830090/1‑150 (MQ=255)

                                                                 gAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAg                                                                                      <  2:86131/150‑1 (MQ=255)

                                                                  aaCCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGc                                                                                     >  2:3472189/1‑150 (MQ=255)

                                                                    ccGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGt                                                                                   >  2:3743891/1‑150 (MQ=255)

                                                                       ggTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCtt                                                                                >  1:3662611/1‑150 (MQ=255)

                                                                        gTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTg                                                                               <  1:2290454/150‑1 (MQ=255)

                                                                                gAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTg                                                                       <  1:917156/150‑1 (MQ=255)

                                                                                   aaCCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTg                                                                    >  1:4129640/1‑150 (MQ=255)

                                                                                     ccGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGc                                                                  <  1:1577153/150‑1 (MQ=255)

                                                                                      cGCTACATGGATATTTTCAGTGGTGAGGTTGAAGATCAGCCGGGGGTGACGGATATGTCGTCCGCGGTTGACGGTGATTTACAGGGCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTTATTCGTTGGTGGcc                                                                 <  2:721133/150‑1 (MQ=255)

                                                                                       gCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGccc                                                                >  1:4434727/1‑150 (MQ=255)

                                                                                       gCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGccc                                                                <  1:4686340/150‑1 (MQ=255)

                                                                                            aTGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTg                                                           >  1:4215225/1‑150 (MQ=255)

                                                                                            aTGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTg                                                           <  1:410469/150‑1 (MQ=255)

                                                                                            aTGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATGTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTg                                                           <  2:3657325/150‑1 (MQ=255)

                                                                                             tGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTgg                                                          <  1:4222185/150‑1 (MQ=255)

                                                                                             tGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTgg                                                          <  1:1767929/150‑1 (MQ=255)

                                                                                              gCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGCAATCGAAGTGGCACAACGGAAACAGCGTCATGAGTCGGTGCTGGCCCATCTggg                                                         >  2:3241573/1‑150 (MQ=255)

                                                                                              gCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCCTCTggg                                                         >  2:4005003/1‑150 (MQ=255)

                                                                                              gCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTAACAGCCCCGCGTCGAAACCCAGGAAATCGACGAGGAAGAACAGAAACAGCATCTAGAAACGAAGCAGGCCCAACTggg                                                         >  2:3241690/1‑150 (MQ=255)

                                                                                               cATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGga                                                        <  1:2127445/150‑1 (MQ=255)

                                                                                                ctcgtgtCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGTCGGATATGTCGCCCTCGGTTGACGGTGATGTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTGGCTGGCCCATCTGGgag                                                       <  2:4888700/144‑1 (MQ=255)

                                                                                                aTCTTTTCTGTGTTGAGGTTGATGATCAGCCGGCGGTGTGGGATATGTCGCACGCGGTTGACGGTGATTTACTGGCCCGCGTCGAAGCCCTGGACATCGTAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGgag                                                       <  2:2707626/150‑1 (MQ=255)

                                                                                                  cTTGTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGAt                                                     <  2:3827853/150‑1 (MQ=255)

                                                                                                    tttCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTa                                                   <  2:3262252/150‑1 (MQ=255)

                                                                                                      tCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTaaa                                                 <  1:567145/150‑1 (MQ=255)

                                                                                                      tCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCGGGGAGATTaaa                                                 >  1:3162890/1‑150 (MQ=255)

                                                                                                       cAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAg                                                <  2:4966559/150‑1 (MQ=255)

                                                                                                       cAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAg                                                >  2:81744/1‑150 (MQ=255)

                                                                                                        aGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGt                                               >  2:4457670/1‑150 (MQ=255)

                                                                                                         gtggtgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTg                                              >  2:4591400/1‑150 (MQ=255)

                                                                                                         gtggtgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTg                                              <  2:1831654/150‑1 (MQ=255)

                                                                                                          tggtgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGa                                             >  2:2637486/1‑150 (MQ=255)

                                                                                                             tgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaa                                          >  1:4148558/1‑150 (MQ=255)

                                                                                                             tgAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaa                                          <  1:4005425/150‑1 (MQ=255)

                                                                                                              gAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaaa                                         >  2:4704150/1‑150 (MQ=255)

                                                                                                               aGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaaaa                                        <  1:2238906/150‑1 (MQ=255)

                                                                                                                 gTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAAtt                                      <  2:2218525/150‑1 (MQ=255)

                                                                                                                 gTTGAAGAACAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAAtt                                      <  2:2926519/150‑1 (MQ=255)

                                                                                                                   tGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTAc                                    <  2:3904588/150‑1 (MQ=255)

                                                                                                                         tCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCg                              >  1:5026516/1‑150 (MQ=255)

                                                                                                                          ccgCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCgg                             <  1:2606672/148‑1 (MQ=255)

                                                                                                                          cAGCCGGCGGTGACGGATATGTCGAACTCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCgg                             >  1:1420523/1‑150 (MQ=255)

                                                                                                                          cAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCgg                             <  2:468808/150‑1 (MQ=255)

                                                                                                                          cAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCgg                             >  2:1274788/1‑150 (MQ=255)

                                                                                                                            gCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCg                           <  1:1365452/150‑1 (MQ=255)

                                                                                                                               ggcggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAg                        <  1:2340428/150‑1 (MQ=255)

                                                                                                                               ggcggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAg                        <  1:3331946/150‑1 (MQ=255)

                                                                                                                                gcggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGt                       <  1:1563644/150‑1 (MQ=255)

                                                                                                                                 cggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTg                      <  1:277191/150‑1 (MQ=255)

                                                                                                                                 cggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTg                      <  1:1666671/150‑1 (MQ=255)

                                                                                                                                  ggTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCGGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTgg                     >  2:104178/1‑150 (MQ=255)

                                                                                                                                   gTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTggg                    >  2:4316820/1‑150 (MQ=255)

                                                                                                                                       cGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTa                >  1:4573573/1‑150 (MQ=255)

                                                                                                                                         gATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAgg              >  1:220985/1‑150 (MQ=255)

                                                                                                                                          atatGTCGAACGCTGTTGACGGTGATTTACAGGCCCGCGTCGTAGCCCTTGAAATCGAAGTGGCATAACTGAAACAGCGTCTTGATTCGTTGCTGTCCCATCTGGGAGATTAAAGTGAAAAAATTAAGTATCTGCGTAGTGGGATTAGGt             <  2:80179/150‑1 (MQ=255)

                                                                                                                                          atatGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGt             >  1:691044/1‑150 (MQ=255)

                                                                                                                                          atatGTCGAACGCGGTTGACGGTGATGTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGt             <  2:4303702/150‑1 (MQ=255)

                                                                                                                                               tCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCAt        <  2:4144309/150‑1 (MQ=255)

                                                                                                                                                  aaCGCGGGTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTgc     <  2:1137429/150‑1 (MQ=255)

TGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGCTACATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCA  >  CP009273/1122010‑1122308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`