BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,129,182	G→A	8.3%	A385T (GCC→ACC)	flgE →	flagellar hook protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,129,182	0	G	A	8.3%	1129.3 / 64.6	335	A385T (GCC→ACC)	flgE	flagellar hook protein
Reads supporting (aligned to +/- strand): ref base G (139/168); new base A (19/9); total (158/177)
Fisher's exact test for biased strand distribution p-value = 2.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

Only 100 of 338 total aligned reads displayed.

CGACGCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTA  >  CP009273/1129033‑1129330

cGACGCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACg                                                                                                                                                      <  1:3015024/150‑1 (MQ=255)

cGACGCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACg                                                                                                                                                      <  2:4077532/150‑1 (MQ=255)

   cGCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGccc                                                                                                                                                   <  2:238681/150‑1 (MQ=255)

    gCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCa                                                                                                                                                  <  2:2317552/150‑1 (MQ=255)

      aaTCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGa                                                                                                                                                >  1:671615/1‑150 (MQ=255)

      aaTCTTCTGGCGTGGCGCTGGTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGa                                                                                                                                                >  2:2039059/1‑150 (MQ=255)

       aTCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGAc                                                                                                                                               >  2:130472/1‑150 (MQ=255)

       aTCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGAc                                                                                                                                               <  1:1804003/150‑1 (MQ=255)

          ttctGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCAt                                                                                                                                            >  1:708608/1‑150 (MQ=255)

                cgtggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAAc                                                                                                                                      <  1:4985128/150‑1 (MQ=255)

                cgtggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAAc                                                                                                                                      <  1:2528392/150‑1 (MQ=255)

                cgtggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAAc                                                                                                                                      <  2:3550255/150‑1 (MQ=255)

                cgtggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACCGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAAc                                                                                                                                      <  1:1174690/150‑1 (MQ=255)

                 gtggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAAcc                                                                                                                                     >  1:1550735/1‑150 (MQ=255)

                  tggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAAccc                                                                                                                                    >  2:2469874/1‑150 (MQ=255)

                   ggcgCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCa                                                                                                                                   >  2:3825802/1‑150 (MQ=255)

                    gcgcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAg                                                                                                                                  <  1:4730131/150‑1 (MQ=255)

                     cgcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAgg                                                                                                                                 <  1:1438049/150‑1 (MQ=255)

                      gcTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGa                                                                                                                                >  1:122082/1‑150 (MQ=255)

                              ggACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATcc                                                                                                                        <  1:3462797/150‑1 (MQ=255)

                                aCAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTc                                                                                                                      <  1:4011059/150‑1 (MQ=255)

                                    ccGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATATCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAaca                                                                                                                  <  2:4783263/150‑1 (MQ=255)

                                     cgggacgggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAacac                                                                                                                 <  2:2235120/150‑1 (MQ=255)

                                         acgggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTg                                                                                                             <  2:1686589/150‑1 (MQ=255)

                                           gggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGt                                                                                                           >  2:4835053/1‑150 (MQ=255)

                                           gggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGt                                                                                                           <  1:3044495/150‑1 (MQ=255)

                                           gggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGt                                                                                                           <  1:2159760/150‑1 (MQ=255)

                                            ggaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGtt                                                                                                          >  1:2642050/1‑150 (MQ=255)

                                             gaAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTa                                                                                                         <  1:421214/150‑1 (MQ=255)

                                              aaaCTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTaa                                                                                                        >  1:2591553/1‑150 (MQ=255)

                                              aaaCTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTaa                                                                                                        >  2:3294022/1‑150 (MQ=255)

                                               aaCTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAAc                                                                                                       >  2:4889311/1‑150 (MQ=255)

                                                 cTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACtt                                                                                                     <  1:543573/150‑1 (MQ=255)

                                                    tGGCACCCTGACCATCGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACg                                                                                                  <  2:5079266/150‑1 (MQ=255)

                                                    tGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACg                                                                                                  <  1:2806025/150‑1 (MQ=255)

                                                        aCCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTaa                                                                                              >  1:1580354/1‑150 (MQ=255)

                                                         cacTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAAt                                                                                             <  1:4486767/148‑1 (MQ=255)

                                                          ccTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATc                                                                                            <  1:3247155/150‑1 (MQ=255)

                                                          ccTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATc                                                                                            <  1:3913438/150‑1 (MQ=255)

                                                          ccTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATc                                                                                            <  1:4836876/150‑1 (MQ=255)

                                                           cTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCg                                                                                           >  1:3582484/1‑150 (MQ=255)

                                                            tGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGc                                                                                          >  2:2545864/1‑150 (MQ=255)

                                                             gACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCt                                                                                         >  2:1635918/1‑150 (MQ=255)

                                                             gACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCt                                                                                         <  1:3107587/150‑1 (MQ=255)

                                                              aCCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTg                                                                                        >  2:3391394/1‑150 (MQ=255)

                                                               ccAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGa                                                                                       <  1:1529663/150‑1 (MQ=255)

                                                                    ggTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGa                                                                                  >  1:1460497/1‑150 (MQ=255)

                                                                         gcTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCt                                                                             >  2:2880535/1‑150 (MQ=255)

                                                                          cTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTc                                                                            >  2:4576636/1‑150 (MQ=255)

                                                                           tGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCa                                                                           >  2:4416638/1‑150 (MQ=255)

                                                                           tGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCa                                                                           >  2:427936/1‑150 (MQ=255)

                                                                             gAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAAt                                                                         <  1:4163797/150‑1 (MQ=255)

                                                                             gAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAAt                                                                         <  2:212017/150‑1 (MQ=255)

                                                                             gAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAAt                                                                         <  1:5085995/150‑1 (MQ=255)

                                                                              aaGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATg                                                                        >  2:3043933/1‑150 (MQ=255)

                                                                              aaGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATg                                                                        >  1:3347666/1‑150 (MQ=255)

                                                                                 cGTCCACCGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGAt                                                                     <  1:3171093/150‑1 (MQ=255)

                                                                                 cGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGAt                                                                     >  1:4769791/1‑150 (MQ=255)

                                                                                 cGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGAt                                                                     >  1:5168365/1‑150 (MQ=255)

                                                                                  gTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATc                                                                    <  2:4346615/150‑1 (MQ=255)

                                                                                   tCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCa                                                                   <  2:2406752/150‑1 (MQ=255)

                                                                                    ccAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCAc                                                                  >  1:1976479/1‑150 (MQ=255)

                                                                                     cAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGGAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACg                                                                 >  2:557355/1‑150 (MQ=255)

                                                                                      aaCGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGc                                                                >  2:1846715/1‑150 (MQ=255)

                                                                                         gTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAAt                                                             >  2:367053/1‑150 (MQ=255)

                                                                                         gTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAAt                                                             >  1:3843225/1‑150 (MQ=255)

                                                                                         gTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGACAAACGCCCAGACCAACAAAACCCAGGACCAGATCCTCAACACGCAGGTAAACTTACGCTAATACCTGACGGCATACCTCAATGTATCACTCaag                                                             >  2:2696476/1‑149 (MQ=255)

                                                                                          tCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAAtt                                                            <  1:748028/150‑1 (MQ=255)

                                                                                             aTCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTtat                                                         <  1:5081099/150‑1 (MQ=255)

                                                                                             aTCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTtat                                                         >  1:787303/1‑150 (MQ=255)

                                                                                             aTCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACACCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTtat                                                         >  1:10152/1‑150 (MQ=255)

                                                                                               ctcAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATAc                                                       >  2:2869742/1‑150 (MQ=255)

                                                                                                  aGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACcgc                                                    >  1:4708745/1‑150 (MQ=255)

                                                                                                     aaaGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGAt                                                 <  1:3517729/150‑1 (MQ=255)

                                                                                                     aaaGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGAt                                                 <  2:3876685/150‑1 (MQ=255)

                                                                                                     aaaGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGAt                                                 <  2:2772761/150‑1 (MQ=255)

                                                                                                      aaGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGTTAGCTCAATGGATCACGCAATTTATACCGCGATg                                                <  2:192484/150‑1 (MQ=255)

                                                                                                        gAACTGGTCAAGATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCATGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATggg                                              <  2:1578200/150‑1 (MQ=255)

                                                                                                         aaCTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGa                                             <  2:5210590/150‑1 (MQ=255)

                                                                                                         aaCTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGa                                             <  1:4398684/150‑1 (MQ=255)

                                                                                                          aCTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGag                                            >  2:2430347/1‑150 (MQ=255)

                                                                                                          aCTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGAACATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGag                                            <  2:4943745/150‑1 (MQ=255)

                                                                                                           cTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGagc                                           >  1:1079486/1‑150 (MQ=255)

                                                                                                              gTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGagcagc                                        >  2:1167145/1‑150 (MQ=255)

                                                                                                                cAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGcagcca                                      <  2:3147648/150‑1 (MQ=255)

                                                                                                                  atatGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGcagccagc                                    <  1:4089566/150‑1 (MQ=255)

                                                                                                                   tatGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCagccagcc                                   <  1:459214/150‑1 (MQ=255)

                                                                                                                    atGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAgccagcca                                  <  1:3965280/150‑1 (MQ=255)

                                                                                                                          gTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCTGACACt                            >  2:766657/1‑150 (MQ=255)

                                                                                                                          gTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACt                            >  2:1771643/1‑150 (MQ=255)

                                                                                                                           ttGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTg                           >  1:2843466/1‑150 (MQ=255)

                                                                                                                           ttGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGAAAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTg                           >  1:1773683/1‑150 (MQ=255)

                                                                                                                              cccAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAAt                        <  1:3703306/150‑1 (MQ=255)

                                                                                                                              cccAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAAt                        <  1:1084949/150‑1 (MQ=255)

                                                                                                                                cAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCa                      >  1:2257303/1‑150 (MQ=255)

                                                                                                                                  gCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAAc                    <  2:3094528/150‑1 (MQ=255)

                                                                                                                                     tAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAgg                 >  1:1283761/1‑150 (MQ=255)

                                                                                                                                     tAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAgg                 <  2:4454643/150‑1 (MQ=255)

                                                                                                                                               tCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGc       <  1:3524966/150‑1 (MQ=255)

                                                                                                                                                  aaCGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAg    <  1:2354556/150‑1 (MQ=255)

CGACGCAATCTTCTGGCGTGGCGCTGTTGGGGACAGCCGGGACGGGAAACTTTGGCACCCTGACCAACGGTGCGCTGGAAGCGTCCAACGTCGATCTCAGTAAAGAACTGGTCAATATGATCGTTGCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAACACGCTGGTTAACTTACGCTAATCGCTGACGGGATAGCTCAATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACACTGAATCAACAGGCGGTAACCGCCAGTA  >  CP009273/1129033‑1129330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`