BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,152,278	G→T	72.3%	E16* (GAA→TAA)	ycfH →	putative DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,152,278	0	G	T	72.3%	421.2 / 245.4	278	E16* (GAA→TAA)	ycfH	putative DNase
Reads supporting (aligned to +/- strand): ref base G (38/39); new base T (94/107); total (132/146)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 282 total aligned reads displayed.

GCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGG  >  CP009273/1152131‑1152426

gCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTaa                                                                                                                                                     <  2:311084/150‑1 (MQ=255)

 cATCAACCGCAAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAAt                                                                                                                                                    <  1:490062/150‑1 (MQ=255)

  aTCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATc                                                                                                                                                   >  1:3301425/1‑150 (MQ=255)

   taaaCCGCGAGCTTCTCATCAACGATCTTTTACTGAGTATTGAGGATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACTTCATGTTTTTAGTCGACTCACTCTGCCATCTCTATGGTCTTGATTATGAATCt                                                                                                                                                  <  2:3210751/148‑1 (MQ=255)

   tCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTAGTAATCt                                                                                                                                                  >  1:2734605/1‑150 (MQ=255)

     aaCCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCttt                                                                                                                                                <  1:477510/150‑1 (MQ=255)

         gcgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCAt                                                                                                                                            <  2:4645707/150‑1 (MQ=255)

          cgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATa                                                                                                                                           <  1:1457738/150‑1 (MQ=255)

          cgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATa                                                                                                                                           <  2:2522220/150‑1 (MQ=255)

          cgAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATa                                                                                                                                           <  2:1728294/150‑1 (MQ=255)

           gAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATaa                                                                                                                                          >  1:3598594/1‑150 (MQ=255)

                tctcATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACg                                                                                                                                     <  2:3363226/150‑1 (MQ=255)

                 ctcATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑t                                                                                                                                   >  1:4349426/1‑150 (MQ=255)

                  tcatcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑Tg                                                                                                                                  <  1:2400854/150‑1 (MQ=255)

                    atcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGa                                                                                                                                <  2:966499/150‑1 (MQ=255)

                     tcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGAt                                                                                                                               <  2:1835017/150‑1 (MQ=255)

                     tcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGAt                                                                                                                               <  2:3041781/150‑1 (MQ=255)

                     tcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGAt                                                                                                                               <  1:449508/150‑1 (MQ=255)

                     tcaCCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGAt                                                                                                                               <  2:3878975/150‑1 (MQ=255)

                        ccGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGAc                                                                                                                            >  2:766152/1‑150 (MQ=255)

                          gATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGt                                                                                                                          <  1:3198435/150‑1 (MQ=255)

                          gATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGt                                                                                                                          <  1:3810635/150‑1 (MQ=255)

                          gATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGt                                                                                                                          >  2:1641787/1‑150 (MQ=255)

                           aTCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGtt                                                                                                                         <  1:4368515/150‑1 (MQ=255)

                                ttACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGc                                                                                                                    <  1:627769/150‑1 (MQ=255)

                                   cTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGaa                                                                                                                 >  1:4436298/1‑150 (MQ=255)

                                    tGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGaaa                                                                                                                >  1:1754453/1‑150 (MQ=255)

                                     gCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAg                                                                                                               <  1:1181648/150‑1 (MQ=255)

                                         aTTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAgccgc                                                                                                           >  1:3443669/1‑150 (MQ=255)

                                         aTTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGAGTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAgccgc                                                                                                           >  2:4573413/1‑150 (MQ=255)

                                          ttGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAgccgcc                                                                                                          >  1:5232971/1‑150 (MQ=255)

                                                 ttACCTGCATCCGGGCGTTGAGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCAcgcg                                                                                                   <  2:4931152/150‑1 (MQ=255)

                                                  tACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGa                                                                                                  >  2:3272019/1‑150 (MQ=255)

                                                        cAACCGGGCGTTGTGCGACCGGTTCCTCATCTATAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCAACTCGATGCACGGCAGTAATAAGCTTTGCAGCAGCACG‑TGCATCACGTTCTGGCGAAAGCCGCCGCCCGCGCTGTGaa                                                                                            >  2:4372875/1‑150 (MQ=255)

                                                         aaCCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGaaa                                                                                           >  1:2315010/1‑150 (MQ=255)

                                                            cGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCTTAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGACAttt                                                                                        >  2:2980908/1‑150 (MQ=255)

                                                            cGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAttt                                                                                        >  2:4139297/1‑150 (MQ=255)

                                                             gggCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAAtttt                                                                                       >  1:5011226/1‑150 (MQ=255)

                                                                    gtgCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGc                                                                                <  1:2185160/150‑1 (MQ=255)

                                                                        tACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTc                                                                            <  2:5237196/150‑1 (MQ=255)

                                                                        tACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATGTTGTCTGGCAGTc                                                                            >  2:336703/1‑150 (MQ=255)

                                                                          ccGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGc                                                                          <  2:4045591/150‑1 (MQ=255)

                                                                          ccGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGc                                                                          <  1:4072461/150‑1 (MQ=255)

                                                                            ggTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCca                                                                        >  2:2837402/1‑150 (MQ=255)

                                                                            ggTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCca                                                                        >  2:4768073/1‑150 (MQ=255)

                                                                              ttCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCcaca                                                                      >  2:4358053/1‑150 (MQ=255)

                                                                              ttCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCcaca                                                                      >  2:1259070/1‑150 (MQ=255)

                                                                                ccTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaac                                                                    <  2:3566951/150‑1 (MQ=255)

                                                                                ccTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaac                                                                    >  1:1035062/1‑150 (MQ=255)

                                                                                 cTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCacaaca                                                                   >  1:3705063/1‑150 (MQ=255)

                                                                                   catatatAAGAGAGACATCATGTTTTAAGTCAACAAACACAGCAATCTCGATGTTATGGAATAATAAACTAAGCAAAAAGACGATGAATAAC‑TTCTGGCGAAATCCGCCGCACGCGATGTGAAATATTGACTGGCATTCGCAACAACAtt                                                                 >  2:2383136/7‑150 (MQ=255)

                                                                                   cATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACAtt                                                                 <  2:3008736/150‑1 (MQ=255)

                                                                                    aTCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTa                                                                <  1:4942175/150‑1 (MQ=255)

                                                                                    aTCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTa                                                                <  1:4298488/150‑1 (MQ=255)

                                                                                     tCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTAc                                                               <  2:2235756/150‑1 (MQ=255)

                                                                                      cTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTAcc                                                              <  1:766152/150‑1 (MQ=255)

                                                                                          aaGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGt                                                          >  2:5003640/1‑150 (MQ=255)

                                                                                           agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGtt                                                         <  2:2758428/150‑1 (MQ=255)

                                                                                           agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGtt                                                         >  2:3247529/1‑150 (MQ=255)

                                                                                           agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGtt                                                         >  2:4671370/1‑150 (MQ=255)

                                                                                           agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGtt                                                         <  2:339244/150‑1 (MQ=255)

                                                                                           agagagACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGtt                                                         <  2:2426668/150‑1 (MQ=255)

                                                                                               agaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAttt                                                     >  2:1559265/1‑150 (MQ=255)

                                                                                               agaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGAGTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTAttt                                                     <  2:212008/150‑1 (MQ=255)

                                                                                                gaCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTa                                                    <  1:1845459/150‑1 (MQ=255)

                                                                                                 aCATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTAc                                                   <  1:3119883/150‑1 (MQ=255)

                                                                                                  catcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACa                                                  >  2:4220697/1‑150 (MQ=255)

                                                                                                  catcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGACGCCACAACATTACCGGGTTATTTACa                                                  >  1:1425108/1‑150 (MQ=255)

                                                                                                   atcatGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACat                                                 <  1:1564991/150‑1 (MQ=255)

                                                                                                     catGTTTTTAGTCGACTCACACTGCCATCTCGAGGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACatat                                               <  2:1594270/150‑1 (MQ=255)

                                                                                                      atGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATg                                              <  2:261553/150‑1 (MQ=255)

                                                                                                              aGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCt                                      >  1:1938332/1‑150 (MQ=255)

                                                                                                              aGTCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCt                                      <  1:1537138/150‑1 (MQ=255)

                                                                                                              aGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCt                                      <  2:664644/150‑1 (MQ=255)

                                                                                                                tCGACTCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCTGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTgg                                    <  1:5213903/150‑1 (MQ=255)

                                                                                                                    cTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGATCTGGCGAAAGCCGCCGAACGCGATGTGAAATGTTGTCTGGCAGTCGCCACAACATTACCGTGTTATTTACATATGCGGGATCTGGAAgg                                >  1:900025/1‑150 (MQ=255)

                                                                                                                     tCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGc                               <  1:2636664/150‑1 (MQ=255)

                                                                                                                     tCACACTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGc                               >  1:1270027/1‑150 (MQ=255)

                                                                                                                     tCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGc                               >  1:1365230/1‑150 (MQ=255)

                                                                                                                      cacaCTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCg                              <  1:3074012/150‑1 (MQ=255)

                                                                                                                      cacaCTGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCg                              >  1:3521687/1‑150 (MQ=255)

                                                                                                                         acTGCCATCTCGATTGTCTGGATTATTAATCTTTTCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCAGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAAc                           <  2:1189000/150‑1 (MQ=255)

                                                                                                                          cTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACg                          >  2:22891/1‑150 (MQ=255)

                                                                                                                           tGCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGt                         <  2:3777698/150‑1 (MQ=255)

                                                                                                                            gCCATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTg                        <  1:4300520/150‑1 (MQ=255)

                                                                                                                             ccATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGa                       <  2:1366282/150‑1 (MQ=255)

                                                                                                                             ccATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGa                       >  2:1793888/1‑150 (MQ=255)

                                                                                                                              cATCTCGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGAc                      >  1:2618798/1‑150 (MQ=255)

                                                                                                                                  tcGATGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcg                  >  1:3897721/1‑150 (MQ=255)

                                                                                                                                     aTGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcg               >  2:5150265/1‑150 (MQ=255)

                                                                                                                                      tGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcgt              >  2:428188/1‑150 (MQ=255)

                                                                                                                                      tGGTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAcgtcgt              >  2:618913/1‑150 (MQ=255)

                                                                                                                                       ggTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTa             <  2:2399689/150‑1 (MQ=255)

                                                                                                                                        gTCTGGATTATTAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTAt            >  2:4018155/1‑150 (MQ=255)

                                                                                                                                              attattAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCtt      <  1:2799952/150‑1 (MQ=255)

                                                                                                                                              aTTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCtt      >  1:2068990/1‑150 (MQ=255)

                                                                                                                                              aTTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCtt      >  1:4672898/1‑150 (MQ=255)

                                                                                                                                              aTTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCtt      >  1:4180164/1‑150 (MQ=255)

                                                                                                                                               ttattaATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTtg     >  1:2913195/1‑150 (MQ=255)

                                                                                                                                                  tGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTgg  <  1:4094207/150‑1 (MQ=255)

GCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACG‑TGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGG  >  CP009273/1152131‑1152426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`