Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,229,460 G→T 17.3% F238L (TTC→TTA)  nhaB ← sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,229,4600GT17.3% 834.0 / 131.8 293F238L (TTC→TTA) nhaBsodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (112/130);  new base T (34/17);  total (146/147)
Fisher's exact test for biased strand distribution p-value = 8.91e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 312 total aligned reads displayed.

CGGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTT  >  CP009273/1229311‑1229609
                                                                                                                                                     |                                                                                                                                                     
cggcTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACCTGCGCAGgaag                                                                                                                                                       >  2:4729144/1‑150 (MQ=255)
cggcTGTGCTCGTCACACGGTAGCACCACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGGAGCCAAACCACCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGgaag                                                                                                                                                       <  2:3962289/150‑1 (MQ=255)
 tgcTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGgaaga                                                                                                                                                      <  1:2075956/149‑1 (MQ=255)
   cTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATaaa                                                                                                                                                    >  1:3624863/1‑150 (MQ=255)
   cTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGACAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGaaa                                                                                                                                                    >  2:2788437/1‑150 (MQ=255)
     ttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATc                                                                                                                                                  <  1:4251157/150‑1 (MQ=255)
     ttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATc                                                                                                                                                  <  2:1493683/150‑1 (MQ=255)
      tGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCg                                                                                                                                                 >  2:650519/1‑150 (MQ=255)
      tGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACAGGCGCAGGAATAAATCg                                                                                                                                                 >  2:623050/1‑150 (MQ=255)
        aTCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGcc                                                                                                                                               >  1:3734751/1‑150 (MQ=255)
          cGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTCACAGGCCACAAATCAGAACCGGACCGGTCACCGGCGACATGCGCAGGAAGAAATCGCCaa                                                                                                                                             >  1:2810038/1‑150 (MQ=255)
               aaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCAACGGCGACATGCGCAGGAAGAAATCGCCAAAATgc                                                                                                                                        >  1:2161184/1‑150 (MQ=255)
                 cTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgcca                                                                                                                                      >  1:600967/1‑150 (MQ=255)
                 cTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgcca                                                                                                                                      <  1:1310325/150‑1 (MQ=255)
                  tGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgccag                                                                                                                                     <  2:4652031/150‑1 (MQ=255)
                   gTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATgccagc                                                                                                                                    >  1:2898755/1‑150 (MQ=255)
                   gTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgccagc                                                                                                                                    >  2:921923/1‑150 (MQ=255)
                       cAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCcagccagc                                                                                                                                >  1:2287069/1‑150 (MQ=255)
                        aaCACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCagccagcc                                                                                                                               <  2:4605980/150‑1 (MQ=255)
                            cTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCtt                                                                                                                           <  1:768635/150‑1 (MQ=255)
                              tCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTa                                                                                                                         <  2:5028158/150‑1 (MQ=255)
                                gcgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGc                                                                                                                       <  1:1615251/150‑1 (MQ=255)
                                   gACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgat                                                                                                                    >  1:2781195/1‑150 (MQ=255)
                                     cTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatga                                                                                                                  >  2:680815/1‑150 (MQ=255)
                                        tctcCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCa                                                                                                               >  1:3518884/1‑150 (MQ=255)
                                           ccGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGt                                                                                                            <  1:4809899/150‑1 (MQ=255)
                                           ccGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGt                                                                                                            >  1:2147383/1‑150 (MQ=255)
                                            cGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGtt                                                                                                           <  1:3436121/150‑1 (MQ=255)
                                            cGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGtt                                                                                                           <  2:1343909/150‑1 (MQ=255)
                                             ggCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCCCCGGCGACATGCGCCGGAAGAAATCGCCAAAATGCCAGCCAGCCGCGTTAGCGATGATCAGGTTc                                                                                                          >  1:5027293/1‑150 (MQ=255)
                                             ggCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTc                                                                                                          <  2:1833755/150‑1 (MQ=255)
                                              gCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCCGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCt                                                                                                         <  1:973976/150‑1 (MQ=255)
                                              gCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCt                                                                                                         <  1:1037746/150‑1 (MQ=255)
                                              gCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCt                                                                                                         <  1:4584871/150‑1 (MQ=255)
                                              gCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCt                                                                                                         <  1:3861091/150‑1 (MQ=255)
                                                aGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCtgt                                                                                                       <  2:4911463/150‑1 (MQ=255)
                                                 tcGTTTCACCGGAGCCAAACCAACGCATCTTCTCGACCAGCAGGCAGGTTAACAGTCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCTAAATGCCAGCCTGCCGCTTTTGAGATGATCAGGTTCtgtg                                                                                                      <  2:3990637/149‑1 (MQ=255)
                                                 gCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCtgtg                                                                                                      <  1:4296434/150‑1 (MQ=255)
                                                 gCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCtgtg                                                                                                      <  1:2397962/150‑1 (MQ=255)
                                                   gTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGt                                                                                                    >  2:1593972/1‑150 (MQ=255)
                                                   gTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGt                                                                                                    >  1:3341829/1‑150 (MQ=255)
                                                     ttCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCTATGATCAGGTTCTGTGGTTc                                                                                                  <  2:3538202/150‑1 (MQ=255)
                                                     ttCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTc                                                                                                  <  1:1369813/150‑1 (MQ=255)
                                                     ttCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTc                                                                                                  <  2:930054/150‑1 (MQ=255)
                                                        aCCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGcc                                                                                               >  1:1511785/1‑150 (MQ=255)
                                                        aCCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGcc                                                                                               <  2:338264/150‑1 (MQ=255)
                                                        aCCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGcc                                                                                               <  2:2976924/150‑1 (MQ=255)
                                                            tAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGCCATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCccacc                                                                                           >  2:2528254/1‑150 (MQ=255)
                                                             aGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCccacca                                                                                          >  2:3160652/1‑150 (MQ=255)
                                                               ccAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATg                                                                                        <  1:4717616/150‑1 (MQ=255)
                                                                cAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATgg                                                                                       >  1:125359/1‑150 (MQ=255)
                                                                 aaaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGt                                                                                      >  2:2368552/1‑150 (MQ=255)
                                                                 aaaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGt                                                                                      <  1:4093092/150‑1 (MQ=255)
                                                                 aaaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGt                                                                                      <  2:2286912/150‑1 (MQ=255)
                                                                  aaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGACGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTc                                                                                     >  2:4848163/1‑150 (MQ=255)
                                                                   aCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTGTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCa                                                                                    >  2:1234029/1‑150 (MQ=255)
                                                                        cGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcg                                                                               <  2:857709/150‑1 (MQ=255)
                                                                        cGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcg                                                                               >  2:1681213/1‑150 (MQ=255)
                                                                          cAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgcc                                                                             <  1:488639/150‑1 (MQ=255)
                                                                          cAGCGTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgcc                                                                             <  2:2664720/150‑1 (MQ=255)
                                                                           aGCTTCTCTACCAGCAGGCATGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGAGGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgccg                                                                            <  2:4361719/150‑1 (MQ=255)
                                                                            gCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgccgc                                                                           >  1:3230273/1‑150 (MQ=255)
                                                                                      cagcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGt                                                                 <  1:1422351/150‑1 (MQ=255)
                                                                                      cagcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGt                                                                 >  1:1696035/1‑150 (MQ=255)
                                                                                        gcaggcagGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGc                                                               >  1:2653477/1‑150 (MQ=255)
                                                                                         caggcaggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGcc                                                              <  1:3462634/150‑1 (MQ=255)
                                                                                              aggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCAATCCAGCCGCTTTAGCGATGATCAGTTTCTGTGGATCGCACACCATGGTCATTACGCCGCCTAATGAGGTGCCGacac                                                         >  2:2896545/1‑150 (MQ=255)
                                                                                               ggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACAcc                                                        >  2:1397261/1‑150 (MQ=255)
                                                                                               ggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACAcc                                                        <  2:221912/150‑1 (MQ=255)
                                                                                                    aCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgcat                                                   <  2:1961946/150‑1 (MQ=255)
                                                                                                           acaAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatcat                                            >  2:4141999/1‑150 (MQ=255)
                                                                                                           acaAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatcat                                            >  1:4409360/1‑150 (MQ=255)
                                                                                                            caAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCatcatc                                           >  1:201468/1‑150 (MQ=255)
                                                                                                              aaTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAg                                         <  1:527629/150‑1 (MQ=255)
                                                                                                               aTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAgg                                        >  2:1120629/1‑150 (MQ=255)
                                                                                                               aTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAgg                                        <  1:1922522/150‑1 (MQ=255)
                                                                                                                tCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGc                                       >  2:1788388/1‑150 (MQ=255)
                                                                                                                  aGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTa                                     <  2:4005736/150‑1 (MQ=255)
                                                                                                                  aGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTa                                     <  2:1284900/150‑1 (MQ=255)
                                                                                                                      ccGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCa                                 >  2:3767725/1‑150 (MQ=255)
                                                                                                                       cGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAg                                <  2:3579302/150‑1 (MQ=255)
                                                                                                                        ggAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGa                               >  2:5181781/1‑150 (MQ=255)
                                                                                                                         taaCGGTCACCGGAGACATGCGCATGTAGAAATCGCCATAATGCCTGCCAGCCGATTTAGCGTTTATCAGGTTCTGTGGTTCGGCCACCATGGTCAGTTCGCCGCCTTATGCGGTGCCGACACCGGCATGCTTCATCTGGCTACGCAGaa                              <  2:2875835/149‑1 (MQ=255)
                                                                                                                            cGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGc                           <  1:2595268/150‑1 (MQ=255)
                                                                                                                            cGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGc                           <  1:5103877/150‑1 (MQ=255)
                                                                                                                             ggTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGcc                          <  2:5098902/150‑1 (MQ=255)
                                                                                                                               tCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCAc                        <  2:719539/150‑1 (MQ=255)
                                                                                                                               tCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCAc                        <  2:2478323/150‑1 (MQ=255)
                                                                                                                                   cGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGaa                    <  2:1787240/150‑1 (MQ=255)
                                                                                                                                      cGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTg                 >  2:237987/1‑150 (MQ=255)
                                                                                                                                       gACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGt                >  2:4275846/1‑150 (MQ=255)
                                                                                                                                       gACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGt                >  1:925843/1‑150 (MQ=255)
                                                                                                                                        aCATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGtt               >  1:2427188/1‑150 (MQ=255)
                                                                                                                                          aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTcc             >  1:1148216/1‑150 (MQ=255)
                                                                                                                                           tGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCa            <  1:3086685/150‑1 (MQ=255)
                                                                                                                                             cgcAGGAATAAATCGCCAAAATGCCAGCCAGACGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGa          >  2:1581917/1‑150 (MQ=255)
                                                                                                                                             cgcAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGa          >  1:4041610/1‑150 (MQ=255)
                                                                                                                                             cgcAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGa          <  1:2373202/150‑1 (MQ=255)
                                                                                                                                                aGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAcc       >  1:853739/1‑150 (MQ=255)
                                                                                                                                                     gaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACttt  >  1:2478125/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTTT  >  CP009273/1229311‑1229609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑