Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,506,689 A→T 5.5% Q260L (CAG→CTG)  ydcS → polyhydroxybutyrate (PHB) synthase, ABC transporter periplasmic binding protein homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,506,6890AT 5.5% 1304.6 / 44.6 366Q260L (CAG→CTG) ydcSpolyhydroxybutyrate (PHB) synthase, ABC transporter periplasmic binding protein homolog
Reads supporting (aligned to +/- strand):  ref base A (191/155);  new base T (8/12);  total (199/167)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 376 total aligned reads displayed.

GTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCATTAACGCCAA  >  CP009273/1506540‑1506838
                                                                                                                                                     |                                                                                                                                                     
gTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCa                                                                                                                                                       <  2:1341067/150‑1 (MQ=255)
gTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCa                                                                                                                                                       <  1:1019428/150‑1 (MQ=255)
  aTCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAgg                                                                                                                                                     >  2:4670812/1‑150 (MQ=255)
   tCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCTGGc                                                                                                                                                    >  1:4209994/1‑150 (MQ=255)
     aaCTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCa                                                                                                                                                  <  1:4120696/150‑1 (MQ=255)
         cACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGc                                                                                                                                              >  1:551237/1‑150 (MQ=255)
           ccGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGccc                                                                                                                                            <  2:2445528/150‑1 (MQ=255)
              aagaaCAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGa                                                                                                                                         >  2:1578744/1‑150 (MQ=255)
               agaaCAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGaa                                                                                                                                        >  1:514977/1‑150 (MQ=255)
               agaaCAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGaa                                                                                                                                        >  1:1106414/1‑150 (MQ=255)
                  ccAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGc                                                                                                                                     <  2:4468437/149‑1 (MQ=255)
                    aGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCg                                                                                                                                   >  1:2985667/1‑150 (MQ=255)
                    aGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCg                                                                                                                                   >  2:2062436/1‑150 (MQ=255)
                    aGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCg                                                                                                                                   >  1:3595795/1‑150 (MQ=255)
                      tACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGaa                                                                                                                                 >  2:2927902/1‑150 (MQ=255)
                       aCCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAg                                                                                                                                <  1:274684/150‑1 (MQ=255)
                        ccAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAgg                                                                                                                               >  2:1840899/1‑150 (MQ=255)
                          aGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGcc                                                                                                                             >  2:1279908/1‑150 (MQ=255)
                          aGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGcc                                                                                                                             >  1:4191838/1‑150 (MQ=255)
                            gCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCTGTGCCTGGCCCTATCTGGCCAACGCCCTGAAAGCCGAAGGCCAg                                                                                                                           <  1:954476/150‑1 (MQ=255)
                              ggTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCCGcc                                                                                                                         >  2:4406223/1‑150 (MQ=255)
                              ggTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGcc                                                                                                                         <  1:1392850/150‑1 (MQ=255)
                              ggTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGcc                                                                                                                         <  2:139356/150‑1 (MQ=255)
                              ggTGCTGAAAGTGCTGCGCGCTCAACACAGTGTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCGATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGcc                                                                                                                         >  1:3419850/1‑150 (MQ=255)
                                  cTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGtt                                                                                                                     <  1:1172665/150‑1 (MQ=255)
                                          gctgcGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGt                                                                                                             >  2:1769547/1‑150 (MQ=255)
                                           ctgcTCGCTCAACAGAGTTTGATCCTTCGCTTCTGGCATGACTCTTCCGTGCAAATGTGCGATTTCATAATCGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGACCTGATAGACGATGTCCTGCCTGTTGGTACCGtt                                                                                                            <  2:1494553/150‑1 (MQ=255)
                                           ctgcGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGtt                                                                                                            >  1:1471845/1‑150 (MQ=255)
                                            tgcGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGttt                                                                                                           >  2:787706/1‑150 (MQ=255)
                                              cgcgcTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGGGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGATGGCCAGCCTGTTGCTACCGTTTTc                                                                                                         >  2:3956668/1‑150 (MQ=255)
                                               gcgcACAACAAAGTTTTAAACATAGCAACTTGAATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGAACTCGTTGATACCAGTGACTGGCCCTATCTGGCCACCGCCCAGAAAGCCGAATGACAGACTGTAGCTAACGtggtcc                                                                                                        >  2:3672263/1‑145 (MQ=255)
                                                ctcTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTccc                                                                                                       <  2:1218755/148‑1 (MQ=255)
                                                 gcTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCg                                                                                                      >  2:4038215/1‑150 (MQ=255)
                                                 gcTCAACACAGGTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCg                                                                                                      >  2:687693/1‑150 (MQ=255)
                                                   tCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGaa                                                                                                    >  1:2007883/1‑150 (MQ=255)
                                                         caGTTTGATCCTTCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAggg                                                                                              <  1:2333570/150‑1 (MQ=255)
                                                          aGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGgt                                                                                             <  1:4901796/150‑1 (MQ=255)
                                                           gTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGgtg                                                                                            <  1:5065848/150‑1 (MQ=255)
                                                             ttGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCTGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGtt                                                                                          >  1:1805258/1‑150 (MQ=255)
                                                                aTCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTAcc                                                                                       >  2:147335/1‑150 (MQ=255)
                                                                aTCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTAcc                                                                                       >  2:2651740/1‑150 (MQ=255)
                                                                 tCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCg                                                                                      >  1:4095418/1‑150 (MQ=255)
                                                                  ccATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCgg                                                                                     >  1:81765/1‑150 (MQ=255)
                                                                   cATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGt                                                                                    >  1:1071810/1‑150 (MQ=255)
                                                                   cATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGt                                                                                    >  1:3845419/1‑150 (MQ=255)
                                                                    aTCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGtt                                                                                   >  2:2134425/1‑150 (MQ=255)
                                                                      cGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTgg                                                                                 >  1:1973504/1‑150 (MQ=255)
                                                                      cGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTgg                                                                                 <  2:3096609/150‑1 (MQ=255)
                                                                      cGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTgg                                                                                 >  1:306175/1‑150 (MQ=255)
                                                                        cTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGc                                                                               >  1:369840/1‑150 (MQ=255)
                                                                         tACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCt                                                                              <  1:2042042/150‑1 (MQ=255)
                                                                         tACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCt                                                                              <  2:603015/150‑1 (MQ=255)
                                                                          aCTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTg                                                                             <  1:3981417/150‑1 (MQ=255)
                                                                            tGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGAt                                                                           <  1:169697/150‑1 (MQ=255)
                                                                              gCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATac                                                                         >  2:2891882/1‑150 (MQ=255)
                                                                                     acTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCAtg                                                                  >  2:4574282/1‑150 (MQ=255)
                                                                                          cGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCa                                                             >  2:5149154/1‑150 (MQ=255)
                                                                                          cGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCa                                                             <  2:3718763/150‑1 (MQ=255)
                                                                                           gTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCAt                                                            <  2:3338933/150‑1 (MQ=255)
                                                                                            tGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATa                                                           <  1:4507185/150‑1 (MQ=255)
                                                                                             gCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATag                                                          <  1:349044/150‑1 (MQ=255)
                                                                                             gCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATag                                                          <  1:1760418/150‑1 (MQ=255)
                                                                                              cAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATagc                                                         <  1:3156016/150‑1 (MQ=255)
                                                                                               aaaTGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATagcg                                                        <  2:5078111/150‑1 (MQ=255)
                                                                                                  tGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCTGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATagcgaag                                                     <  1:4585449/150‑1 (MQ=255)
                                                                                                   gAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATagcgaagc                                                    <  2:2242842/150‑1 (MQ=255)
                                                                                                      cGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAgcgaagcgaa                                                 <  1:923339/150‑1 (MQ=255)
                                                                                                      cGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAgcgaagcgaa                                                 <  2:2013918/150‑1 (MQ=255)
                                                                                                      cGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAgcgaagcgaa                                                 <  2:4067675/150‑1 (MQ=255)
                                                                                                       naTTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGaaa                                                >  2:3812063/2‑150 (MQ=255)
                                                                                                            cAAAAACGAGGGTGTGTTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCATGAAAGCAGAAGGACAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACAACCATGCTGCATAGCGAAGCGAAACATcc                                           >  2:2980438/1‑150 (MQ=255)
                                                                                                            cAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATcc                                           >  1:608271/1‑150 (MQ=255)
                                                                                                             aacaaCGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCg                                          <  2:1691688/147‑1 (MQ=255)
                                                                                                             aaaaaCGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCg                                          <  2:2536911/150‑1 (MQ=255)
                                                                                                              aaaaCGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCgg                                         >  1:1234698/1‑150 (MQ=255)
                                                                                                               aaaCGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGt                                        >  1:1582300/1‑150 (MQ=255)
                                                                                                                  cGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTg                                     >  1:4392506/1‑150 (MQ=255)
                                                                                                                  cGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTg                                     >  1:2490295/1‑150 (MQ=255)
                                                                                                                   gAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTgc                                    <  2:1345625/150‑1 (MQ=255)
                                                                                                                     gggTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTgcgc                                  >  1:4674501/1‑150 (MQ=255)
                                                                                                                       gtgtGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCt                                >  1:3891450/1‑150 (MQ=255)
                                                                                                                         gtgGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTAc                              <  2:2396612/150‑1 (MQ=255)
                                                                                                                         gtgGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTAc                              <  2:4989751/150‑1 (MQ=255)
                                                                                                                             ttGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACACAt                          >  1:1028190/1‑150 (MQ=255)
                                                                                                                             ttGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAAt                          >  1:3264155/1‑150 (MQ=255)
                                                                                                                               gCTTCCATTTCCTGGCACTATCAGGCCTACGCCCTGAAAGCAGAAGGCCATCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTTGATAGCGAAGCGAAACATCCGGTTTGAGCCTACAAATgg                        <  2:3422453/150‑1 (MQ=255)
                                                                                                                               gCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATgg                        <  2:3422306/150‑1 (MQ=255)
                                                                                                                                cTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGa                       >  2:1111586/1‑150 (MQ=255)
                                                                                                                                  tCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATg                     <  1:2928138/150‑1 (MQ=255)
                                                                                                                                   ccAGTGCCTGGCCCTATCTGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGa                    >  1:1554659/1‑150 (MQ=255)
                                                                                                                                    cAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGaa                   >  1:314558/1‑150 (MQ=255)
                                                                                                                                      gTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACt                 <  1:499952/150‑1 (MQ=255)
                                                                                                                                            ggCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCAt           >  1:1788112/1‑150 (MQ=255)
                                                                                                                                            ggCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCAt           <  2:3493769/150‑1 (MQ=255)
                                                                                                                                            ggCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCAt           >  1:2144388/1‑150 (MQ=255)
                                                                                                                                                  aTCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCATTAACGc     <  1:4231506/150‑1 (MQ=255)
                                                                                                                                                    cAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCATTAACGCCa   >  2:4724541/1‑150 (MQ=255)
                                                                                                                                                    cAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTGACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCATTAACGCCa   >  2:3228425/1‑150 (MQ=255)
                                                                                                                                                     aGGCCACCGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTGTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCATTAACGCCaa  <  1:2822113/150‑1 (MQ=255)
                                                                                                                                                     aGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATCCCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCATTAACGCCaa  >  2:4891868/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGAAGGAGGGTGTTACCGGTTGGGCTGATACCACCATGCTGCATAGCGAAGCGAAACATCCGGTTTGCGCCTACAAATGGATGAACTGGTCATTAACGCCAA  >  CP009273/1506540‑1506838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑