BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,524,654	G→T	18.7%	W159L (TGG→TTG)	ydcD →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,524,654	0	G	T	18.7%	832.4 / 153.3	313	W159L (TGG→TTG)	ydcD	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (127/127); new base T (30/29); total (157/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 316 total aligned reads displayed.

TATGGAGTTCTGCGCTACAAAACAACGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTT  >  CP009273/1524505‑1524803

    gAGTTCTGCGCTACAAAACAACGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCa                                                                                                                                                   <  2:2000677/150‑1 (MQ=255)

    gAGTTCTGCGCTACAAAACAACGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCa                                                                                                                                                   <  2:4604578/150‑1 (MQ=255)

      gTTCTGCGCTACAAAACAACGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATa                                                                                                                                                 >  2:1071818/1‑150 (MQ=255)

         cTGCGCTACAAAACAACGGGTGTATTTTAGTGATAAAAACAAGAAGTCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATg                                                                                                                                              <  2:2392124/150‑1 (MQ=255)

         cTGCGCTACAAAACAACGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATg                                                                                                                                              >  1:920967/1‑150 (MQ=255)

              cTACAAAACAACGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGaacaa                                                                                                                                         <  2:1627502/150‑1 (MQ=255)

               tACAAAACAACGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGaacaac                                                                                                                                        >  1:178650/1‑150 (MQ=255)

                    aacaacGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATGATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAg                                                                                                                                   <  2:1469369/150‑1 (MQ=255)

                     acaacGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGa                                                                                                                                  >  1:1526968/1‑150 (MQ=255)

                         cGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAaga                                                                                                                              <  2:300081/150‑1 (MQ=255)

                          gggTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAagag                                                                                                                             >  2:668714/1‑150 (MQ=255)

                            gtgtATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGATaa                                                                                                                           >  2:4993025/1‑150 (MQ=255)

                            gtgtATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGaa                                                                                                                           <  1:300723/150‑1 (MQ=255)

                              gtATTTTAGTGATAAAAACAAGAAGGCTAGTTATCAAATAATTAATTATGGAGACTATTATGATGTTGATTAGTATGATAACAATTTGAAAAATGAAGTTTTGGACTGGATTGGTCAATGGTCATAATGTACGACACCCGCAAGAGaaaa                                                                                                                         >  2:4540002/1‑150 (MQ=255)

                              gtATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGaaaa                                                                                                                         >  2:1933966/1‑150 (MQ=255)

                                aTTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAat                                                                                                                       >  1:2161629/1‑150 (MQ=255)

                                  tttAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAatat                                                                                                                     >  1:3027761/1‑150 (MQ=255)

                                   ttAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAAtata                                                                                                                    >  1:5160595/1‑150 (MQ=255)

                                    tAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATaa                                                                                                                   >  2:2187206/1‑150 (MQ=255)

                                    tAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATaa                                                                                                                   >  1:3933011/1‑150 (MQ=255)

                                    tAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATaa                                                                                                                   >  1:261566/1‑150 (MQ=255)

                                     aGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATCTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATaaa                                                                                                                  >  1:1032833/1‑150 (MQ=255)

                                      gTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATaaaa                                                                                                                 <  1:4083801/150‑1 (MQ=255)

                                        gATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATCAAata                                                                                                               >  2:884994/1‑150 (MQ=255)

                                          tAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATATTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAataca                                                                                                             <  1:451261/150‑1 (MQ=255)

                                           aaaaaCAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAatacat                                                                                                            <  1:3576181/150‑1 (MQ=255)

                                            aaaaCAAGAATGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATTTAAAatacata                                                                                                           <  2:807865/150‑1 (MQ=255)

                                            aaaaCAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAatacata                                                                                                           <  2:4276285/150‑1 (MQ=255)

                                             aaaCAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAatacatac                                                                                                          >  1:194484/1‑150 (MQ=255)

                                                    tatgCTAGTTATAAAATAATTAATTATGGATACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGa                                                                                                   <  2:4827627/147‑1 (MQ=255)

                                                    aagGATATTTATAAAATAATTAATTTTGGAGACTATTATGTTGTTGATTATTATTATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGa                                                                                                   <  2:2228272/150‑1 (MQ=255)

                                                       gCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACAt                                                                                                <  1:1946140/150‑1 (MQ=255)

                                                          aGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATgag                                                                                             >  1:106511/1‑150 (MQ=255)

                                                          aGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATgag                                                                                             >  1:1908151/1‑150 (MQ=255)

                                                            ttATAAAATAATTAATTATGGAGAATATTATGATTTTGATTATTATTATAACAATTTTAAAAATGAAGTTTTTGACTGGATTGGTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGagag                                                                                           <  1:2752094/150‑1 (MQ=255)

                                                                aaaaTAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAg                                                                                       >  1:2504798/1‑150 (MQ=255)

                                                                aaaaTAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAg                                                                                       >  1:5096322/1‑150 (MQ=255)

                                                                aaaaTAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAg                                                                                       >  1:1307675/1‑150 (MQ=255)

                                                                aaaaTAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAAAAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAg                                                                                       >  2:711730/1‑150 (MQ=255)

                                                                  aataatTATTTATGGATAATATTAAGATGTTTATTATTATGATAAAAATTTGAATAATGAAGTTTTTGACTGGATTGTTAAATGGTCATATTGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAggg                                                                                     <  2:4878525/150‑1 (MQ=255)

                                                                    taattaatTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGAc                                                                                   <  1:415163/150‑1 (MQ=255)

                                                                     aattaattATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACg                                                                                  <  2:1424168/150‑1 (MQ=255)

                                                                     aattaattATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATAAAGAAAGACATGAGAGCAAGGGACg                                                                                  >  2:537194/1‑150 (MQ=255)

                                                                     aattaattATGGAGAATATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACg                                                                                  <  2:2870754/150‑1 (MQ=255)

                                                                         aattaTGGAGACTATTATGATTTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGgggc                                                                              <  2:2650947/150‑1 (MQ=255)

                                                                           ttaTGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGgggcag                                                                            >  2:5157146/1‑150 (MQ=255)

                                                                             aTGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGgggcaggg                                                                          >  2:433716/1‑150 (MQ=255)

                                                                             aTGGAGAATATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGgggcaggg                                                                          <  1:4360315/150‑1 (MQ=255)

                                                                               ggAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGgggcagggca                                                                        >  1:2340217/1‑150 (MQ=255)

                                                                                gagaCTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCaa                                                                       >  1:535650/1‑150 (MQ=255)

                                                                                gagaCTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGAAAGGGACGGGGCAGGGCaa                                                                       >  2:3152382/1‑150 (MQ=255)

                                                                                 agaCTATTATGATGTTGATTATTATGATAACAATTTGAAAAATTAAGTTTTTGACTGGATTTGTAAATTTTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAg                                                                      <  2:4142212/150‑1 (MQ=255)

                                                                                 agaCTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAg                                                                      <  1:245341/150‑1 (MQ=255)

                                                                                  gaCTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGa                                                                     >  2:1719069/1‑150 (MQ=255)

                                                                                   aatattatGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACAGGAGAGCAAGGGACGGGGCAGGGCAAGAt                                                                    >  2:36911/3‑150 (MQ=255)

                                                                                   aCTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGAt                                                                    >  2:1963440/1‑150 (MQ=255)

                                                                                   aCTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGAt                                                                    >  2:37113/1‑150 (MQ=255)

                                                                                   aCTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACAGGAGAGCAAGGGACGGGGCAGGGCAAGAt                                                                    >  1:1161734/1‑150 (MQ=255)

                                                                                    cTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATc                                                                   >  2:1272541/1‑150 (MQ=255)

                                                                                    cTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATc                                                                   <  1:4008947/150‑1 (MQ=255)

                                                                                       ttatGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATg                                                                >  1:3592256/1‑150 (MQ=255)

                                                                                        tatGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGa                                                               <  2:2513629/150‑1 (MQ=255)

                                                                                        tatGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGa                                                               <  1:2288679/150‑1 (MQ=255)

                                                                                         atgatgTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGaa                                                              >  1:2229173/1‑150 (MQ=255)

                                                                                          tgatgTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGaaa                                                             >  2:4618132/1‑150 (MQ=255)

                                                                                          tgatgTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGaaa                                                             <  2:5186233/150‑1 (MQ=255)

                                                                                              gTTTATTATTATTATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAACACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtg                                                         <  1:4722816/150‑1 (MQ=255)

                                                                                              gTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtg                                                         <  1:839885/150‑1 (MQ=255)

                                                                                               ttGATTATTATTATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtgt                                                        <  1:4573650/150‑1 (MQ=255)

                                                                                               ttGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtgt                                                        >  2:682998/1‑150 (MQ=255)

                                                                                                 gATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGa                                                      >  1:3610674/1‑150 (MQ=255)

                                                                                                      ttatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTaa                                                 <  1:1585695/150‑1 (MQ=255)

                                                                                                      ttatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTaa                                                 <  2:2089960/150‑1 (MQ=255)

                                                                                                         tGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATc                                              <  1:3139006/150‑1 (MQ=255)

                                                                                                          gATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCa                                             <  1:3715856/150‑1 (MQ=255)

                                                                                                           aTAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGACAATTGTGATGTAAATCAc                                            >  1:3098034/1‑150 (MQ=255)

                                                                                                            tAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACg                                           >  1:4800084/1‑150 (MQ=255)

                                                                                                              aCAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAATTATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACTAt                                         >  2:3304535/1‑150 (MQ=255)

                                                                                                                  tttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTc                                     <  1:1260441/150‑1 (MQ=255)

                                                                                                                    tGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCAt                                   <  1:2794759/150‑1 (MQ=255)

                                                                                                                     gAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATc                                  >  1:1449622/1‑150 (MQ=255)

                                                                                                                          aTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGc                             >  1:3813500/1‑150 (MQ=255)

                                                                                                                             aattttttGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCttt                          <  2:4658610/147‑1 (MQ=255)

                                                                                                                              aGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTa                         >  2:952441/1‑150 (MQ=255)

                                                                                                                               gTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTaa                        <  2:3369961/150‑1 (MQ=255)

                                                                                                                                  tttGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGc                     >  1:1725844/1‑150 (MQ=255)

                                                                                                                                    tGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGACCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCt                   <  2:5070991/150‑1 (MQ=255)

                                                                                                                                      aCTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTAc                 <  1:5083775/150‑1 (MQ=255)

                                                                                                                                        tGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAg               >  1:4494610/1‑150 (MQ=255)

                                                                                                                                         tgATTGGTAAATGGACAAAAAGAACCACACCACAAAGAGAAACATATAAAATACATACAGAACCACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAgg              <  2:3556425/149‑1 (MQ=255)

                                                                                                                                         ggATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAgg              <  1:2891453/150‑1 (MQ=255)

                                                                                                                                          gtttGGTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGt             <  2:4281567/148‑1 (MQ=255)

                                                                                                                                            ttGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGa           <  2:2113869/150‑1 (MQ=255)

                                                                                                                                             tGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGAt          >  1:2699625/1‑150 (MQ=255)

                                                                                                                                              ggTAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATCCAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATc         <  2:4409638/150‑1 (MQ=255)

                                                                                                                                              ggTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATc         <  2:608396/150‑1 (MQ=255)

                                                                                                                                                tAAATTGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaa       >  2:3876572/1‑150 (MQ=255)

                                                                                                                                                  aaTGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaaaa     >  1:3314270/1‑150 (MQ=255)

                                                                                                                                                   aTGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAAt    <  2:4081208/150‑1 (MQ=255)

                                                                                                                                                    tGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAAtt   <  1:668714/150‑1 (MQ=255)

TATGGAGTTCTGCGCTACAAAACAACGGGTGTATTTTAGTGATAAAAACAAGAAGGCTAGTTATAAAATAATTAATTATGGAGACTATTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTT  >  CP009273/1524505‑1524803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`