Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,784,836 A→T 10.3% V222V (GTT→GTA)  ydiU ← UPF0061 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,784,8360AT10.3% 1080.8 / 88.9 330V222V (GTT→GTA) ydiUUPF0061 family protein
Reads supporting (aligned to +/- strand):  ref base A (148/148);  new base T (19/15);  total (167/163)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 348 total aligned reads displayed.

AAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGG  >  CP009273/1784688‑1784985
                                                                                                                                                    |                                                                                                                                                     
 agtAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGacaac                                                                                                                                                     >  1:2176416/3‑150 (MQ=255)
  aTAAAACCGGGTTCGTAATCATCAAGAAAACCAACCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGacaaca                                                                                                                                                    <  2:2842580/150‑1 (MQ=255)
    aaaaCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATc                                                                                                                                                  >  1:2331189/1‑150 (MQ=255)
     aaaCCGGTTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCg                                                                                                                                                 <  1:1014422/150‑1 (MQ=255)
     aaaCCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCg                                                                                                                                                 <  1:4405599/150‑1 (MQ=255)
       aCCGTGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCt                                                                                                                                               <  2:1515260/150‑1 (MQ=255)
        ccGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTa                                                                                                                                              <  1:2866018/150‑1 (MQ=255)
         cGGGTTCGTAATCATCAAGAAAACCACACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTaa                                                                                                                                             <  2:3069336/150‑1 (MQ=255)
            gTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAcc                                                                                                                                          >  1:3645227/1‑150 (MQ=255)
              tCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCAGGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAAcccg                                                                                                                                        <  2:417378/150‑3 (MQ=255)
               cGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCaga                                                                                                                                       >  2:736331/1‑150 (MQ=255)
               cGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCaga                                                                                                                                       >  2:1637321/1‑150 (MQ=255)
                gTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCagag                                                                                                                                      >  2:1816167/1‑150 (MQ=255)
                gTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACAACGCTAAACCagag                                                                                                                                      >  2:4771978/1‑150 (MQ=255)
                 tAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAgaga                                                                                                                                     >  2:5193671/1‑150 (MQ=255)
                  aaTCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGAc                                                                                                                                    >  2:4512311/1‑150 (MQ=255)
                    tcatcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACgg                                                                                                                                  <  2:263178/150‑1 (MQ=255)
                    tcatcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACgg                                                                                                                                  >  1:4480231/1‑150 (MQ=255)
                    tcatcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACgg                                                                                                                                  <  2:573729/150‑1 (MQ=255)
                     catcaAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGt                                                                                                                                 <  2:3762362/150‑1 (MQ=255)
                        caAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTAtt                                                                                                                              >  2:3665916/1‑150 (MQ=255)
                         aaGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTAttt                                                                                                                             >  2:5238302/1‑150 (MQ=255)
                           gAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGt                                                                                                                           <  1:234545/150‑1 (MQ=255)
                            aaaaCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTc                                                                                                                          >  2:1286321/1‑150 (MQ=255)
                             aaaCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTcc                                                                                                                         >  2:2459028/1‑150 (MQ=255)
                             aaaCCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTcc                                                                                                                         <  2:1381072/150‑1 (MQ=255)
                                 cAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGTGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcat                                                                                                                     <  2:2240032/150‑1 (MQ=255)
                                  aaaCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatc                                                                                                                    <  1:1002631/150‑1 (MQ=255)
                                     cGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCatcatc                                                                                                                 >  1:4349241/1‑150 (MQ=255)
                                      ggCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCt                                                                                                                >  2:2340364/1‑150 (MQ=255)
                                       gCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTg                                                                                                               <  2:322612/150‑1 (MQ=255)
                                       gCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTAGTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGGCCTCATCATCTg                                                                                                               <  2:149743/150‑1 (MQ=255)
                                           gTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAg                                                                                                           <  2:1250019/150‑1 (MQ=255)
                                             aaTCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGAt                                                                                                         >  2:866042/1‑150 (MQ=255)
                                              aTCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGAtg                                                                                                        >  2:4756624/1‑150 (MQ=255)
                                               tCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGAtgt                                                                                                       >  2:1020712/1‑150 (MQ=255)
                                                    cGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCa                                                                                                  <  1:2021935/150‑1 (MQ=255)
                                                      tCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGt                                                                                                >  2:4557713/1‑150 (MQ=255)
                                                        aGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTaa                                                                                              >  2:1472268/1‑150 (MQ=255)
                                                        aGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTaa                                                                                              >  1:1924331/1‑150 (MQ=255)
                                                        aGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTaa                                                                                              >  2:1340633/1‑150 (MQ=255)
                                                           cccAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGa                                                                                           <  1:2681367/150‑1 (MQ=255)
                                                             cagcagCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACg                                                                                         <  1:2268601/150‑1 (MQ=255)
                                                                 agcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATa                                                                                     <  1:1205160/150‑1 (MQ=255)
                                                                 agcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATa                                                                                     >  1:3466624/1‑150 (MQ=255)
                                                                   cGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGc                                                                                   <  1:1348034/150‑1 (MQ=255)
                                                                    gACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCa                                                                                  >  1:3591756/1‑150 (MQ=255)
                                                                    gACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCa                                                                                  >  2:4441345/1‑150 (MQ=255)
                                                                     aCATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCaa                                                                                 >  2:334651/1‑150 (MQ=255)
                                                                        tgttgtCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGt                                                                              <  1:1580136/150‑1 (MQ=255)
                                                                             tCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCt                                                                         <  1:1308301/150‑1 (MQ=255)
                                                                               ggTATTCATCACCCCATGAGCAAAGCAGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTaa                                                                       <  1:313151/150‑1 (MQ=255)
                                                                                gTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAAc                                                                      >  1:3924545/1‑150 (MQ=255)
                                                                                gTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAAc                                                                      <  2:1924331/150‑1 (MQ=255)
                                                                                    tcatcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGAc                                                                  >  2:196956/1‑150 (MQ=255)
                                                                                    tcatcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGAc                                                                  >  1:3387744/1‑150 (MQ=255)
                                                                                      atcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGa                                                                >  1:4951596/1‑150 (MQ=255)
                                                                                      atcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGa                                                                >  1:3187712/1‑150 (MQ=255)
                                                                                       tcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGaa                                                               >  1:2003585/1‑150 (MQ=255)
                                                                                         aCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAcc                                                             <  1:1233298/150‑1 (MQ=255)
                                                                                            ccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCttt                                                          >  1:5088146/1‑150 (MQ=255)
                                                                                            ccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCttt                                                          <  2:3518581/150‑1 (MQ=255)
                                                                                             cATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCtttt                                                         <  1:814585/150‑1 (MQ=255)
                                                                                                gAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCg                                                      <  1:5028779/150‑1 (MQ=255)
                                                                                                 aGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGa                                                     <  2:758948/150‑1 (MQ=255)
                                                                                                     aaGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTcg                                                 >  1:5243129/1‑150 (MQ=255)
                                                                                                     aaGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTcg                                                 >  2:2753920/1‑150 (MQ=255)
                                                                                                         cGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCgcggc                                             >  2:3993771/1‑150 (MQ=255)
                                                                                                           aCCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGcggcgg                                           <  1:736331/150‑1 (MQ=255)
                                                                                                             cGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgta                                         <  1:4200487/150‑1 (MQ=255)
                                                                                                              gTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgtag                                        <  2:3452364/150‑1 (MQ=255)
                                                                                                               tCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgtagt                                       <  1:3829655/150‑1 (MQ=255)
                                                                                                               tCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgtagt                                       <  1:4152543/150‑1 (MQ=255)
                                                                                                                cTGCCATTGGGCAATTAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgtagta                                      >  1:1380957/1‑150 (MQ=255)
                                                                                                                 tGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTaa                                     <  1:3226021/150‑1 (MQ=255)
                                                                                                                 tGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTaa                                     >  1:4694995/1‑150 (MQ=255)
                                                                                                                    cATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTaaaaa                                  >  2:1752202/1‑150 (MQ=255)
                                                                                                                     aTTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAAt                                 <  2:3004247/150‑1 (MQ=255)
                                                                                                                      ttGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATg                                <  1:4386367/150‑1 (MQ=255)
                                                                                                                       tGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGt                               <  2:3808480/150‑1 (MQ=255)
                                                                                                                       tGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGt                               <  2:4200886/150‑1 (MQ=255)
                                                                                                                        gggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGtt                              <  1:4441345/150‑1 (MQ=255)
                                                                                                                           cAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGAAGAACCTTTTCCGACTCGCGGAGGTAGTAAAAATGTTCGa                           >  1:2412745/1‑150 (MQ=255)
                                                                                                                               tAACGACGCGGTACGTGCGACTACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATg                       >  2:948865/1‑150 (MQ=255)
                                                                                                                               tAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATg                       <  2:5006015/150‑1 (MQ=255)
                                                                                                                                  cgacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGAcc                    >  2:2675654/1‑150 (MQ=255)
                                                                                                                                  cgacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGAcc                    >  2:2252007/1‑150 (MQ=255)
                                                                                                                                     cgcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCaaa                 <  1:4618087/150‑1 (MQ=255)
                                                                                                                                      gcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAg                <  2:3902933/150‑1 (MQ=255)
                                                                                                                                       cgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAgc               >  1:2581425/1‑150 (MQ=255)
                                                                                                                                       cgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAgc               <  1:384068/150‑1 (MQ=255)
                                                                                                                                        ggTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAGATGTTCGAAATGACCAAAgcg              >  2:3637743/1‑150 (MQ=255)
                                                                                                                                        ggTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAgcg              <  1:1531271/150‑1 (MQ=255)
                                                                                                                                          tACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCa            >  1:3563332/1‑150 (MQ=255)
                                                                                                                                           aCGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAg           <  1:3541933/150‑1 (MQ=255)
                                                                                                                                             gTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGAt         <  2:3147970/150‑1 (MQ=255)
                                                                                                                                               gCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGa       <  1:782352/150‑1 (MQ=255)
                                                                                                                                                 gACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACt     <  2:4309092/150‑1 (MQ=255)
                                                                                                                                                 gACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACt     >  1:4760160/1‑150 (MQ=255)
                                                                                                                                                  acaacaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACtt    >  1:595758/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAATAAAACCGGGTTCGTAATCATCAAGAAAACCAAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGG  >  CP009273/1784688‑1784985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑