Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,832,413 C→A 52.3% L315L (CTC→CTA)  ynjC → inner membrane putative ABC superfamily transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,832,4130CA52.3% 20.2 / inf 347L315L (CTC→CTA) ynjCinner membrane putative ABC superfamily transporter permease
Reads supporting (aligned to +/- strand):  ref base C (65/100);  new base A (88/94);  total (153/194)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 367 total aligned reads displayed.

ACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTAC  >  CP009273/1832264‑1832560
                                                                                                                                                     |                                                                                                                                                   
  tATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCTACGATCAATAGTGAAGCGCtcat                                                                                                                                                   >  1:3457446/1‑150 (MQ=255)
  tATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtcat                                                                                                                                                   >  1:2522059/1‑150 (MQ=255)
  tATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtcat                                                                                                                                                   >  1:1738400/1‑150 (MQ=255)
  tATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtaat                                                                                                                                                   >  1:3726839/1‑150 (MQ=255)
  tATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGGGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtcat                                                                                                                                                   >  1:5113461/1‑150 (MQ=255)
    ttCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtcatca                                                                                                                                                 <  2:1508786/150‑1 (MQ=255)
    ttCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtcatca                                                                                                                                                 <  2:3657468/150‑1 (MQ=255)
    ttCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtcatca                                                                                                                                                 >  2:3162636/1‑150 (MQ=255)
      ccccGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAAc                                                                                                                                               >  1:3067329/1‑150 (MQ=255)
       cccGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACa                                                                                                                                              <  1:2282831/150‑1 (MQ=255)
         cgcgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGc                                                                                                                                            >  2:1674249/1‑150 (MQ=255)
         cgcgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGc                                                                                                                                            <  2:4075004/150‑1 (MQ=255)
          gcgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGcc                                                                                                                                           <  2:1963382/150‑1 (MQ=255)
          gcgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGcc                                                                                                                                           <  2:4568111/150‑1 (MQ=255)
             tAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGTGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGa                                                                                                                                        <  2:2422489/150‑1 (MQ=255)
              aGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGAc                                                                                                                                       >  2:482860/1‑150 (MQ=255)
              aGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGAc                                                                                                                                       >  2:2322837/1‑150 (MQ=255)
                    cGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATggg                                                                                                                                 <  2:727867/150‑1 (MQ=255)
                     gTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATgggg                                                                                                                                >  1:754086/1‑150 (MQ=255)
                     gTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATgggg                                                                                                                                >  1:4077898/1‑150 (MQ=255)
                     gTTCGCAAGCCTGCCACGCCTTGATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATgggg                                                                                                                                <  2:3511990/150‑1 (MQ=255)
                       tCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCt                                                                                                                              <  2:1744547/150‑1 (MQ=255)
                       tCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCt                                                                                                                              <  1:4612057/150‑1 (MQ=255)
                                  ccACGCCTTTATTGCCGGGCAATACGCTTGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGACGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACAt                                                                                                                   <  2:2796875/150‑1 (MQ=255)
                                       ccTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCCGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATc                                                                                                              >  1:3946612/1‑150 (MQ=255)
                                           tATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCtt                                                                                                          >  2:570729/1‑150 (MQ=255)
                                             ttGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTg                                                                                                        >  2:2871086/1‑150 (MQ=255)
                                                ccGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTc                                                                                                     <  2:4454926/150‑1 (MQ=255)
                                                   ggCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTg                                                                                                  <  1:5241742/150‑1 (MQ=255)
                                                     cAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGGGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGGCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGtt                                                                                                >  2:1621666/1‑150 (MQ=255)
                                                      aaTACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTa                                                                                               <  2:958355/150‑1 (MQ=255)
                                                      aaTACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTAATCGCTTTGCTCCTGTTa                                                                                               >  2:5027328/1‑150 (MQ=255)
                                                      aaTACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTa                                                                                               >  2:4204566/1‑150 (MQ=255)
                                                      aaTACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTCTTa                                                                                               >  2:3767453/1‑150 (MQ=255)
                                                            cTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActgctg                                                                                         >  2:4905122/1‑150 (MQ=255)
                                                            cTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActgctg                                                                                         >  1:248540/1‑150 (MQ=255)
                                                               gCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTgg                                                                                      >  2:4288232/1‑150 (MQ=255)
                                                                 gATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTTGCt                                                                                    <  1:337473/150‑1 (MQ=255)
                                                                 gATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCt                                                                                    >  2:1292980/1‑150 (MQ=255)
                                                                  aTTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACAGTCATCGCTTTGCTCCTGTTACTGCTGTGGCTg                                                                                   >  2:2621321/1‑150 (MQ=255)
                                                                   tttttttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTgg                                                                                  >  1:2157798/1‑150 (MQ=255)
                                                                     tttttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGaa                                                                                <  1:2418441/150‑1 (MQ=255)
                                                                       tttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATg                                                                              <  1:4725917/150‑1 (MQ=255)
                                                                       tttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATg                                                                              >  1:1272980/1‑150 (MQ=255)
                                                                        ttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATgg                                                                             <  1:2098178/150‑1 (MQ=255)
                                                                        ttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATgg                                                                             <  1:5023569/150‑1 (MQ=255)
                                                                           cccTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATggggg                                                                          <  1:4282048/150‑1 (MQ=255)
                                                                           cccTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATggggg                                                                          >  2:3305214/1‑150 (MQ=255)
                                                                                aaCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCcaca                                                                     >  2:4252965/1‑150 (MQ=255)
                                                                                aaCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCcaca                                                                     <  1:4823113/150‑1 (MQ=255)
                                                                                aaCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCcaca                                                                     <  1:4143345/150‑1 (MQ=255)
                                                                                 aCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAg                                                                    <  1:3162636/150‑1 (MQ=255)
                                                                                 aCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAg                                                                    <  1:999358/150‑1 (MQ=255)
                                                                                 aCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAg                                                                    <  1:3516502/150‑1 (MQ=255)
                                                                                 aCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAg                                                                    <  1:1379607/150‑1 (MQ=255)
                                                                                 aCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAg                                                                    <  1:1346370/150‑1 (MQ=255)
                                                                                     gtgtGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTc                                                                <  1:2399219/150‑1 (MQ=255)
                                                                                     gtgtGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTc                                                                <  1:4518580/150‑1 (MQ=255)
                                                                                     gtgtGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTc                                                                <  1:146175/150‑1 (MQ=255)
                                                                                         gCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCa                                                            <  1:852369/150‑1 (MQ=255)
                                                                                          cTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAg                                                           <  1:3665413/150‑1 (MQ=255)
                                                                                           tgtgtgtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGt                                                          <  2:2563695/150‑1 (MQ=255)
                                                                                            gtgtgtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGtt                                                         <  1:3642168/150‑1 (MQ=255)
                                                                                                gtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTgg                                                     >  1:4484388/1‑150 (MQ=255)
                                                                                                  ggTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCACCAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCt                                                   <  2:2481597/150‑1 (MQ=255)
                                                                                                  ggTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCt                                                   >  2:809591/1‑150 (MQ=255)
                                                                                                  ggTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCt                                                   >  2:1090356/1‑150 (MQ=255)
                                                                                                      ctgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCTGTTGTGGCTATgg                                               <  1:3880511/150‑1 (MQ=255)
                                                                                                       tgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGt                                              <  1:69987/150‑1 (MQ=255)
                                                                                                        gctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGtt                                             <  2:119923/150‑1 (MQ=255)
                                                                                                        gctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGtt                                             <  1:1166488/150‑1 (MQ=255)
                                                                                                         ctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTg                                            <  1:1283438/150‑1 (MQ=255)
                                                                                                         ctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGACGCCAGTTGTGGCTATGGTTg                                            >  1:509388/1‑150 (MQ=255)
                                                                                                          tgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGc                                           >  1:2818078/1‑150 (MQ=255)
                                                                                                              gATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCAt                                       <  1:3112471/150‑1 (MQ=255)
                                                                                                               aTCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTCTGGTTGCCCagt                                      >  2:2716460/1‑148 (MQ=255)
                                                                                                                tCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCAttt                                     <  1:2697637/150‑1 (MQ=255)
                                                                                                                 ccTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACCGCGTCGCCAGTTGTGGCTATGGTTGCCCAtttt                                    <  2:3007297/150‑1 (MQ=255)
                                                                                                                      cgGAACAGACGACTATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGTCTGGTGGCGACATTCATCGCTTTGCTCCGGTTACTGCTGTGGCTGGAATGGGGGCCAAATCGTCTCCAGTTGTGGCTATGGTTGCCCATATTATTAc                               >  2:2202555/1‑150 (MQ=255)
                                                                                                                        gATCAGTCGACGATCAATAGAGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCt                             <  2:4690055/150‑1 (MQ=255)
                                                                                                                         aTCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTg                            <  1:1073688/150‑1 (MQ=255)
                                                                                                                          tCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGc                           <  1:2370111/150‑1 (MQ=255)
                                                                                                                          tCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGc                           >  1:2745604/1‑150 (MQ=255)
                                                                                                                             gtagacgaTCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGctct                        <  2:3227780/147‑1 (MQ=255)
                                                                                                                             gTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGctct                        <  1:4396727/150‑1 (MQ=255)
                                                                                                                             gTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGctct                        <  1:3196279/150‑1 (MQ=255)
                                                                                                                               cgacgaTCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGc                      >  1:3858388/1‑150 (MQ=255)
                                                                                                                                gacgaTCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGcc                     <  2:448235/150‑1 (MQ=255)
                                                                                                                                 acgaTCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCa                    <  1:1963905/150‑1 (MQ=255)
                                                                                                                                 acgaTCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCa                    <  1:1848041/150‑1 (MQ=255)
                                                                                                                                  cgaTCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCAc                   >  2:3876910/1‑150 (MQ=255)
                                                                                                                                  cgaTCAAGAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCAc                   >  2:2473415/1‑150 (MQ=255)
                                                                                                                                    aTCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtg                 >  2:1873541/1‑150 (MQ=255)
                                                                                                                                     tCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACTGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtgg                <  2:4133445/150‑1 (MQ=255)
                                                                                                                                     tCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtgg                >  2:1419340/1‑150 (MQ=255)
                                                                                                                                      cAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtggt               >  1:3608632/1‑150 (MQ=255)
                                                                                                                                        aTAGTGAAGCGCTCATCAACAGCCTGACAATGTGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtggtgg             <  2:3663448/150‑1 (MQ=255)
                                                                                                                                         tAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGc            >  2:677761/1‑150 (MQ=255)
                                                                                                                                               aGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCa      <  1:905812/150‑1 (MQ=255)
                                                                                                                                                  gcTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTa   >  2:3733300/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTAC  >  CP009273/1832264‑1832560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑