Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,909,342 T→A 66.4% E150D (GAA→GAT)  proQ ← RNA chaperone, putative ProP translation regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,909,3420TA66.4% 371.4 / 283.1 270E150D (GAA→GAT) proQRNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand):  ref base T (45/46);  new base A (104/75);  total (149/121)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTG  >  CP009273/1909195‑1909488
                                                                                                                                                   |                                                                                                                                                  
gTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCcatc                                                                                                                                                  <  1:2216663/150‑1 (MQ=255)
gTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCcatc                                                                                                                                                  >  1:1628810/1‑150 (MQ=255)
   ttGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCttt                                                                                                                                               <  2:779007/150‑1 (MQ=255)
    tGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTg                                                                                                                                              <  2:2323375/150‑1 (MQ=255)
     gACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTgc                                                                                                                                             <  2:2090249/150‑1 (MQ=255)
          gCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTGTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCggcgt                                                                                                                                        >  2:4821543/1‑150 (MQ=255)
           cTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCggcgtg                                                                                                                                       >  1:2935973/1‑150 (MQ=255)
           cTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCggcgtg                                                                                                                                       <  2:3999706/150‑1 (MQ=255)
            tttCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTGTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCggcgtgg                                                                                                                                      >  1:2642451/1‑150 (MQ=255)
              tCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGATCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGACATCTTTGCggcgtggcg                                                                                                                                    >  2:646218/1‑150 (MQ=255)
                     cAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCgg                                                                                                                             >  1:4895973/1‑150 (MQ=255)
                      aGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGa                                                                                                                            >  1:4356305/1‑150 (MQ=255)
                       gggCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGAc                                                                                                                           <  1:4107041/150‑1 (MQ=255)
                        ggCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACg                                                                                                                          <  2:609706/150‑1 (MQ=255)
                           ttGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTgg                                                                                                                       >  1:1339262/1‑150 (MQ=255)
                            tGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGc                                                                                                                      >  2:4637434/1‑150 (MQ=255)
                            tGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGc                                                                                                                      >  2:889438/1‑150 (MQ=255)
                              tCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCtt                                                                                                                    >  1:2111140/1‑150 (MQ=255)
                              tCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCtt                                                                                                                    >  1:1503807/1‑150 (MQ=255)
                               ccGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTg                                                                                                                   >  1:2850922/1‑150 (MQ=255)
                                  aCAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGt                                                                                                                >  2:3228604/1‑150 (MQ=255)
                                   cAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGtt                                                                                                               <  1:2729414/150‑1 (MQ=255)
                                   cAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGtt                                                                                                               <  1:1865700/150‑1 (MQ=255)
                                    aGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTc                                                                                                              <  2:5111877/150‑1 (MQ=255)
                                    aGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTc                                                                                                              >  1:3997153/1‑150 (MQ=255)
                                     gTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcg                                                                                                             <  2:30173/150‑1 (MQ=255)
                                     gTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcg                                                                                                             <  2:30223/150‑1 (MQ=255)
                                     gTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcg                                                                                                             <  1:4873466/150‑1 (MQ=255)
                                       cAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTCGCTTGCGTTcgcg                                                                                                           >  2:3631227/1‑150 (MQ=255)
                                         gagCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                                                                                                         >  1:3005524/1‑150 (MQ=255)
                                         gagCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                                                                                                         <  2:3468468/150‑1 (MQ=255)
                                            cTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgcg                                                                                                      >  2:2266327/1‑150 (MQ=255)
                                            cTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgcg                                                                                                      <  1:775101/150‑1 (MQ=255)
                                            cTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgcg                                                                                                      <  2:4893952/150‑1 (MQ=255)
                                            cTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgcg                                                                                                      <  2:1525878/150‑1 (MQ=255)
                                             tGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgcgg                                                                                                     >  1:1062974/1‑150 (MQ=255)
                                                aaTGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGc                                                                                                  <  2:186609/150‑1 (MQ=255)
                                                aaTGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGc                                                                                                  >  1:837633/1‑150 (MQ=255)
                                                       gAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGtcttct                                                                                           >  1:1364907/1‑150 (MQ=255)
                                                        aaaCCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTcttctt                                                                                          >  1:2254294/1‑150 (MQ=255)
                                                              gggTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCAc                                                                                    >  1:2461612/1‑150 (MQ=255)
                                                                 tgtgCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACcag                                                                                 >  2:3915208/1‑150 (MQ=255)
                                                                   tgCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACcagca                                                                               >  1:1517820/1‑150 (MQ=255)
                                                                      tGTTCTTCGCGAGGCGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGtt                                                                            <  2:4791352/150‑1 (MQ=255)
                                                                         tcttcGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCg                                                                         >  2:3538876/1‑150 (MQ=255)
                                                                         tcgtcGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCg                                                                         <  2:585324/147‑1 (MQ=255)
                                                                          cttcGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCTCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCgg                                                                        >  2:3191665/1‑150 (MQ=255)
                                                                          cttcGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCgg                                                                        >  2:3035595/1‑150 (MQ=255)
                                                                                gAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCtt                                                                  >  1:3099411/1‑150 (MQ=255)
                                                                                 aGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTc                                                                 >  2:5008217/1‑150 (MQ=255)
                                                                                  ggTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTcg                                                                <  1:2185410/150‑1 (MQ=255)
                                                                                       ttttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttt                                                           >  1:4588434/1‑150 (MQ=255)
                                                                                       ttttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttt                                                           >  2:1017236/1‑150 (MQ=255)
                                                                                       ttttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttt                                                           >  1:1983341/1‑150 (MQ=255)
                                                                                        tttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttt                                                          <  1:3308086/150‑1 (MQ=255)
                                                                                          tACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttt                                                        <  2:170615/150‑1 (MQ=255)
                                                                                          tACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttt                                                        >  2:2777059/1‑150 (MQ=255)
                                                                                           aCTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTc                                                       >  2:2019870/1‑150 (MQ=255)
                                                                                              gTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCt                                                    >  1:3470772/1‑150 (MQ=255)
                                                                                                ttttGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTg                                                  <  1:3141900/150‑1 (MQ=255)
                                                                                                ttttGGCGCTTTCTCTACCGGATTTTGCGGACGAGGGTTACGGTCAGCGCCATCTTTGCGGCGTGGCGGAGTCGGACGTGGCTTGCGGTCGCGGCGCGGTGCGTCTTCTGTCTCACCAGCTGTTGCGGCAGCTTCGCGTTTTTTCGCTTg                                                  <  2:2855129/150‑1 (MQ=255)
                                                                                                  ttGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCt                                                >  2:4137426/1‑150 (MQ=255)
                                                                                                   tGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTg                                               >  1:1575398/1‑150 (MQ=255)
                                                                                                   tGGCGCTTTCTCGACCGGCTTGTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTg                                               <  2:2952984/150‑1 (MQ=255)
                                                                                                    ggCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGt                                              >  1:643657/1‑150 (MQ=255)
                                                                                                    ggCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGt                                              <  2:1503807/150‑1 (MQ=255)
                                                                                                    ggCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGt                                              >  1:4784834/1‑150 (MQ=255)
                                                                                                      cgcTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTc                                            <  2:3479450/150‑1 (MQ=255)
                                                                                                       gcTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCa                                           <  2:2528981/150‑1 (MQ=255)
                                                                                                            ctctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACg                                      <  2:4649424/150‑1 (MQ=255)
                                                                                                            ctctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACg                                      <  1:2573553/150‑1 (MQ=255)
                                                                                                            ctctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACg                                      >  2:2542280/1‑150 (MQ=255)
                                                                                                            ctctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCAACTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTACTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACg                                      >  2:2598209/1‑150 (MQ=255)
                                                                                                             tctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGc                                     >  2:3745685/1‑150 (MQ=255)
                                                                                                               tACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtg                                   >  1:3677989/1‑150 (MQ=255)
                                                                                                               tACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtg                                   <  2:1777254/150‑1 (MQ=255)
                                                                                                               tACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtg                                   >  1:3795643/1‑150 (MQ=255)
                                                                                                               tACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtg                                   >  1:3680700/1‑150 (MQ=255)
                                                                                                               tACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtg                                   <  2:4386523/150‑1 (MQ=255)
                                                                                                                 ccGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtgtg                                 >  1:3036690/1‑150 (MQ=255)
                                                                                                                  cGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGc                                >  2:332281/1‑150 (MQ=255)
                                                                                                                   ggCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGcc                               <  2:3235699/150‑1 (MQ=255)
                                                                                                                    gCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCt                              >  2:4102767/1‑150 (MQ=255)
                                                                                                                     cTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                             <  1:2055581/150‑1 (MQ=255)
                                                                                                                     cTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                             <  2:3084344/150‑1 (MQ=255)
                                                                                                                     cTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                             <  2:4894271/150‑1 (MQ=255)
                                                                                                                            gcACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcgc                      <  1:5235602/150‑1 (MQ=255)
                                                                                                                             cACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcgcg                     >  1:425280/1‑150 (MQ=255)
                                                                                                                             cACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcgcg                     >  1:2359553/1‑150 (MQ=255)
                                                                                                                                 aGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCttt                 <  2:4227637/150‑1 (MQ=255)
                                                                                                                                  ggTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc                >  2:3621638/1‑150 (MQ=255)
                                                                                                                                  ggTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc                >  2:1774118/1‑150 (MQ=255)
                                                                                                                                  ggTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc                <  1:3180862/150‑1 (MQ=255)
                                                                                                                                   gTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCg               <  1:3056960/150‑1 (MQ=255)
                                                                                                                                        cGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGcttct          <  2:4721034/150‑1 (MQ=255)
                                                                                                                                         gTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGcttctt         <  2:3818340/150‑1 (MQ=255)
                                                                                                                                            cAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCaa      <  2:1476015/150‑1 (MQ=255)
                                                                                                                                             aGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAg     >  2:1243713/1‑150 (MQ=255)
                                                                                                                                             aGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAg     <  1:1940383/150‑1 (MQ=255)
                                                                                                                                               cgcCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgct   <  2:3976342/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTG  >  CP009273/1909195‑1909488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑