Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,094,530 C→T 7.1% V74M (GTG→ATG)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,094,5300CT 7.1% 1165.6 / 51.9 336V74M (GTG→ATG) gnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base C (167/145);  new base T (7/17);  total (174/162)
Fisher's exact test for biased strand distribution p-value = 3.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 340 total aligned reads displayed.

GATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGC  >  CP009273/2094381‑2094679
                                                                                                                                                     |                                                                                                                                                     
    aaGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCAtt                                                                                                                                                   <  2:2392959/150‑1 (MQ=255)
       ttAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAAc                                                                                                                                                >  1:504720/1‑150 (MQ=255)
        tAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACa                                                                                                                                               >  2:678636/1‑150 (MQ=255)
        tAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACa                                                                                                                                               <  2:2967231/150‑1 (MQ=255)
         aaaGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCATCATTAACAg                                                                                                                                              >  2:157810/1‑150 (MQ=255)
           aGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGa                                                                                                                                            >  2:5198467/1‑150 (MQ=255)
            gCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGAt                                                                                                                                           >  1:2639320/1‑150 (MQ=255)
            gCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGAt                                                                                                                                           <  2:3308883/150‑1 (MQ=255)
             cccTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATg                                                                                                                                          <  2:1074719/150‑1 (MQ=255)
                 ctgctgAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGcgac                                                                                                                                      >  1:4242799/1‑150 (MQ=255)
                    ctgAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAg                                                                                                                                   <  2:3588277/150‑1 (MQ=255)
                    ctgAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAg                                                                                                                                   <  2:3055278/150‑1 (MQ=255)
                     tgAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAgg                                                                                                                                  <  1:167254/150‑1 (MQ=255)
                        aaGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCATCATTAACAGGATGCGACGAGGCGt                                                                                                                               <  1:3659838/150‑1 (MQ=255)
                        aaGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGt                                                                                                                               >  1:3800199/1‑150 (MQ=255)
                          gCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTCACAGGATGCGACGAGGCGttt                                                                                                                             >  2:1324531/1‑150 (MQ=255)
                          gCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGttt                                                                                                                             >  2:1455322/1‑150 (MQ=255)
                            tCACGTTTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTcc                                                                                                                           <  2:4788428/150‑1 (MQ=255)
                                gATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCATCATTAACAGGATGCGACGAGGCGTTTCCagag                                                                                                                       <  2:267956/150‑1 (MQ=255)
                                gATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCATCATTAACAGGATGCGACGAGGCGTTTCCagag                                                                                                                       <  2:2060012/150‑1 (MQ=255)
                                gATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCagag                                                                                                                       <  2:4738853/150‑1 (MQ=255)
                                gATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCagag                                                                                                                       <  2:1492832/150‑1 (MQ=255)
                                 aTTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAgaga                                                                                                                      <  2:4667286/150‑1 (MQ=255)
                                   tACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGAtt                                                                                                                    >  1:4766766/1‑150 (MQ=255)
                                    acgacgAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTc                                                                                                                   >  1:7113/1‑150 (MQ=255)
                                     cgacgaATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCg                                                                                                                  <  1:678805/150‑1 (MQ=255)
                                     cgacgaATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCg                                                                                                                  >  2:4096930/1‑150 (MQ=255)
                                           aTAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAAc                                                                                                            >  2:4129813/1‑150 (MQ=255)
                                           aTAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAAc                                                                                                            >  1:1603828/1‑150 (MQ=255)
                                            tAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAAct                                                                                                           <  2:1760026/150‑1 (MQ=255)
                                             aGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAActc                                                                                                          >  2:787542/1‑150 (MQ=255)
                                               tgtCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCtt                                                                                                        >  1:3982694/1‑150 (MQ=255)
                                                gtCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCttt                                                                                                       <  2:4594801/150‑1 (MQ=255)
                                                  ccTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCa                                                                                                     >  1:1418974/1‑150 (MQ=255)
                                                    tGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGCCAATCAAAACCAGCATCCGAGCCTGCACCTGCATACACCAATAACAGGAAGCGACGACGCCATTCCACAGATACGACAAACTCTTTCAcc                                                                                                   >  2:3211959/1‑150 (MQ=255)
                                                      gaagaaGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGAGATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTACACCATTAACAGGATGCGACGAGGCGAATCCAGAGAATCGACAAACACTTTCACCGt                                                                                                 >  1:2498848/1‑150 (MQ=255)
                                                      gaagaaGGTGTTACCACCATCAATGATGATGTCTCCTGTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCATCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGt                                                                                                 <  2:4378573/150‑1 (MQ=255)
                                                       aagaagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGta                                                                                                >  1:1514227/1‑150 (MQ=255)
                                                       aagaagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGta                                                                                                <  2:3325462/150‑1 (MQ=255)
                                                       aagaagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGta                                                                                                >  1:1320722/1‑150 (MQ=255)
                                                        agaagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGtat                                                                                               >  1:3484731/1‑150 (MQ=255)
                                                        agaagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGtat                                                                                               <  1:928365/150‑1 (MQ=255)
                                                         gaagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGtata                                                                                              >  2:4458492/1‑150 (MQ=255)
                                                         gaagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGtata                                                                                              <  2:1651264/150‑1 (MQ=255)
                                                          aagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAg                                                                                             >  1:3890198/1‑150 (MQ=255)
                                                          aagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAg                                                                                             >  2:3332024/1‑150 (MQ=255)
                                                          aagGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAg                                                                                             >  2:4962905/1‑150 (MQ=255)
                                                           agGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGt                                                                                            <  1:3369491/150‑1 (MQ=255)
                                                              tgtTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAg                                                                                         <  2:2454841/150‑1 (MQ=255)
                                                              tgtTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAg                                                                                         <  1:2164218/150‑1 (MQ=255)
                                                                 tACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGaa                                                                                      >  1:5195461/1‑150 (MQ=255)
                                                                  accaccATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAAc                                                                                     >  2:3452735/1‑150 (MQ=255)
                                                                    caccaTCACTTTGGAGGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCa                                                                                   <  2:4909343/150‑1 (MQ=255)
                                                                       caTCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGtt                                                                                <  2:4290783/150‑1 (MQ=255)
                                                                         tCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTc                                                                              <  1:4596205/150‑1 (MQ=255)
                                                                            ctgatgatgTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTg                                                                           <  2:2276686/149‑1 (MQ=255)
                                                                            attatgatgTCTCCGTTATCGAGATATGGTTTGTGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTg                                                                           <  2:273480/147‑1 (MQ=255)
                                                                            atgatgatgTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTg                                                                           <  2:3858781/150‑1 (MQ=255)
                                                                            atgatgatgTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTg                                                                           >  1:2975993/1‑150 (MQ=255)
                                                                                tgatgTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTg                                                                       >  1:1857285/1‑150 (MQ=255)
                                                                                tgatgTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTg                                                                       <  1:896585/150‑1 (MQ=255)
                                                                                tgatgTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTg                                                                       >  2:3360657/1‑150 (MQ=255)
                                                                                  atgTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGa                                                                     >  1:3483123/1‑150 (MQ=255)
                                                                                    gTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGAtt                                                                   >  2:2945760/1‑150 (MQ=255)
                                                                                     tctcCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGAttt                                                                  >  1:4509550/1‑150 (MQ=255)
                                                                                      ctcCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGAtttt                                                                 <  1:591848/150‑1 (MQ=255)
                                                                                            tATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCaa                                                           >  2:4227670/1‑150 (MQ=255)
                                                                                             aTCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCact                                                          >  2:3195380/1‑148 (MQ=255)
                                                                                             aTCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAAt                                                          >  2:565100/1‑150 (MQ=255)
                                                                                                gtgaTATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCAc                                                       <  2:2851333/148‑1 (MQ=255)
                                                                                                gagaTATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCAc                                                       >  1:1180002/1‑150 (MQ=255)
                                                                                                   atatGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCActtc                                                    <  2:3957623/150‑1 (MQ=255)
                                                                                                   atatGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGAGTTTCGGCAATCActtc                                                    >  2:3493335/1‑150 (MQ=255)
                                                                                                    tatGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCActtct                                                   <  1:2016927/150‑1 (MQ=255)
                                                                                                     atGGTGTGAGGGACTCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCActtctt                                                  <  1:2795956/150‑1 (MQ=255)
                                                                                                       ggTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTcc                                                >  2:2566435/1‑150 (MQ=255)
                                                                                                         tttGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGt                                              <  1:863821/150‑1 (MQ=255)
                                                                                                            gAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGtctt                                           <  2:463313/150‑1 (MQ=255)
                                                                                                               ggAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTtctc                                        <  1:4681723/150‑1 (MQ=255)
                                                                                                                 aaTCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCAc                                      <  2:5038029/150‑1 (MQ=255)
                                                                                                                  aTCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACg                                     <  1:1906532/150‑1 (MQ=255)
                                                                                                                     aaTAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGa                                  >  2:1379704/1‑150 (MQ=255)
                                                                                                                     aaTAGCAGCATCCGTGCCTGCACCTGCTTTCACAATTAACAGGATGCGACGAGGCGTTTCCAGAGAGTCGACAAACACTTTCACCGTATAGTAAGGAACCAGATTCTTGCCTGGAATTTCGGCAATCACTTCTTCCGTCTTCTCACGGGa                                  >  1:4274296/1‑150 (MQ=255)
                                                                                                                        agcagcATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGAGTCGACAAACTCTTTCACCGGATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCACTCACTTCTTCCGTCTTCTCACGGGACCg                               >  1:2236000/1‑150 (MQ=255)
                                                                                                                          cagcaTCCGTGCCTGCACCTGCTTTCATCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGt                             <  1:2766626/150‑1 (MQ=255)
                                                                                                                           agcaTCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCACTCACTTCTTCCGTCTTCTCACGGGAACGGtt                            >  2:4872801/1‑150 (MQ=255)
                                                                                                                              aTCCGTGCCTGCACCTGCTTTCATCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGaa                         <  1:4905080/150‑1 (MQ=255)
                                                                                                                              aTCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGaa                         <  1:3070137/150‑1 (MQ=255)
                                                                                                                                 cGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAAt                      <  2:4989179/150‑1 (MQ=255)
                                                                                                                                  gTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATa                     >  1:2985680/1‑150 (MQ=255)
                                                                                                                                   tGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATag                    >  1:497773/1‑150 (MQ=255)
                                                                                                                                       tGCACCTGCTTTCACCATTAACAGGATGCGATGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGAc                <  2:4317631/150‑1 (MQ=255)
                                                                                                                                        gCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACg               >  1:3605540/1‑150 (MQ=255)
                                                                                                                                          aCCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGt             >  2:54217/1‑150 (MQ=255)
                                                                                                                                           ccTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGta            >  1:1000527/1‑150 (MQ=255)
                                                                                                                                             tGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGtata          >  1:2329226/1‑150 (MQ=255)
                                                                                                                                             tGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGtata          >  1:4992019/1‑150 (MQ=255)
                                                                                                                                              gCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATaa         >  1:1722308/1‑150 (MQ=255)
                                                                                                                                              gCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATaa         >  1:3199495/1‑150 (MQ=255)
                                                                                                                                                  tCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCAc     >  2:3780811/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGATGCGACGAGGCGTTTCCAGAGATTCGACAAACTCTTTCACCGTATAGTAAGGAACCAGTTTCTTGCCTGGATTTTCGGCAATCACTTCTTCCGTCTTCTCACGGGAACGGTTGAAAATAGAGACGGTATAACCACGGC  >  CP009273/2094381‑2094679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑