| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 161,171 | G→T | 13.7% | intergenic (+150/‑46) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 161,171 | 0 | G | T | 13.7% | 618.5 / 67.9 | 215 | intergenic (+150/‑46) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base G (101/83); new base T (14/16); total (115/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
Only 100 of 228 total aligned reads displayed.
TTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGA > CP009273/161024‑161320 | ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAAACCACACCCACCAGGGGGAGGGGACCGATCGAGCACAAATTTTGTGAGATATCTTATTATTTATT‑GTAAAAgaataa > 2:1287500/1‑150 (MQ=25) ggttTTCTCCCCCCCCCTGTGGGAGTGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACa < 1:880329/150‑1 (MQ=255) ggttTACTCCCTCTCCCTGTTGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACa < 2:188754/150‑1 (MQ=255) ctccctctccctGGGGGTGTGGTCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAAt < 2:77583/150‑1 (MQ=255) tccctctccctGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATc < 2:5059024/150‑1 (MQ=255) tccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTACACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATc < 2:4496693/150‑1 (MQ=255) ctccctGTGGGATCGGGCCGGGGGGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCt < 2:2406959/150‑1 (MQ=255) ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCACACCCAAACCCACTCCCTCAAGGGCGGGGGGGCCGAACGAGCCCAAATTTTGAGATATATCTTCTTATGGCTTGGTAAC‑TCAGAACAGAAAATCGGGcg > 2:3709407/1‑149 (MQ=255) tccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCtt < 2:4980850/150‑1 (MQ=255) ccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCttt < 1:1589424/150‑1 (MQ=255) cctGGGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCtttt < 1:2926819/150‑1 (MQ=255) cctGGGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCtttt < 2:509332/150‑1 (MQ=255) tgtgggtgtgggCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCTTTTgc < 1:1455012/150‑1 (MQ=255) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGAACCCCCAACCGAACACACACCCCCCAGGGAGAGGGGGCCCCTCCAGCCCCAAATTTGATGGAGATCTTCTTATTTCGT‑TTTACAGAAGAACAGAAAATAGGGCtttttagc > 2:1905691/1‑146 (MQ=255) gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCACGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata < 2:4115143/150‑1 (MQ=255) ggCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAatat < 1:820246/150‑1 (MQ=255) ggCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAatat < 1:3566043/150‑1 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAatat < 2:1668328/150‑1 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAatat < 2:1620343/150‑1 (MQ=255) gCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACACCCTCAAGGGAGAGGGGACCCATCGAGCACAAAATTTGAGAGATATATTATTCTGTCTT‑GTAACAGAATAACACAAAAACGGGCTTTTTCGCCGGAAAAtt > 2:3400817/1‑150 (MQ=255) ccGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCGCCCACAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg < 1:3959733/150‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:799784/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACACCCTCCAGGGAGAGGGGACCGATCGAGCACAAAATTTGAGAGAAATATTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTTCGCCGGAATATTTCg > 2:4146543/1‑150 (MQ=255) gTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGgag < 1:3372666/150‑1 (MQ=255) gccATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:1954365/148‑1 (MQ=255) gactCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACAGATCGAGCACATATTCTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg < 1:4748511/147‑1 (MQ=255) cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATg < 2:3101041/150‑1 (MQ=255) cACGTTCACCCACAACCTAAACCTCTCCCACAAGGGAGAGGGGACCGATCGAGCACAAATTTTTAGAGATATCTTATTATGTCTT‑GTAACAGAAGAAAAGAAAATCGGGCTTTTGCGCCTGAATATTTCGGAGAAAAAGCATGGCCGGGa > 2:2071048/1‑150 (MQ=255) cACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGTATATTGCGGAGAAAAATCATGGcacgga > 2:3253554/1‑145 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGaa < 1:4858246/150‑1 (MQ=255) gTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATg < 2:5152491/150‑1 (MQ=255) gTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATg < 1:1000395/150‑1 (MQ=255) ttCACCCTCACCCGAACCCTCTCCCGCAAGGGAGAGGGGACCGATACAGCCCAAATTTTTAGAGAAATATTAATATGTCTT‑TTTACAGAAGAACAGAAAATCCGGCGTTTGCGCCTGAATATTGCGGAGAAAAAACAAGGCCCGGactta > 2:1105649/1‑146 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGAc < 1:4505683/150‑1 (MQ=255) ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa > 1:2714358/1‑150 (MQ=255) caccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGc > 2:4012917/1‑150 (MQ=255) caccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGc > 1:1354184/1‑150 (MQ=255) ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGACAAAGCATGGCCGGGAATGACCGCGAGCCa < 2:1748666/150‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGACTATTGCGGAGAAAAAGCATGGCCGGGACTGACCGCGAGCCaa > 1:4664563/1‑150 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 1:714392/1‑150 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 2:4617270/1‑150 (MQ=255) ctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAt > 1:3395374/1‑150 (MQ=255) tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt > 1:3890013/1‑150 (MQ=255) tctcCCTCACGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCGTCTGTCTT‑GTAACAGAAGAACAGAAACTCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGc < 1:4839687/150‑1 (MQ=255) cTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAGAAGCATGGCCGGGACTGACCGCGAGCCAATTGGACGCAAAgg > 2:2241447/1‑150 (MQ=255) cTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg > 2:1346538/1‑150 (MQ=255) cTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg > 2:910490/1‑150 (MQ=255) cAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGa < 2:1230215/150‑1 (MQ=255) cAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGa > 1:3868349/1‑150 (MQ=255) aGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCCATTGGACGCAAAGGGaaa > 2:3028988/1‑150 (MQ=255) aGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGaaa > 1:2407237/1‑150 (MQ=255) ggAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAAcc > 1:1793240/1‑150 (MQ=255) gagaGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCg > 1:2074825/1‑150 (MQ=255) gagaGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCg > 1:2074968/1‑150 (MQ=255) gagGGGACCGCTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAGCAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGAc < 2:4811437/150‑1 (MQ=255) gagGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGAc < 2:2591064/150‑1 (MQ=255) gggACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGAcgcg < 1:3195280/150‑1 (MQ=255) aCCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTcc > 1:4973265/1‑150 (MQ=255) ccGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCg < 2:1767924/150‑1 (MQ=255) cGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCgg > 2:1928910/1‑150 (MQ=255) aTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCGTTTGCGCCTGAATATTGCGTAGAAAAAGCATGGCCGGGAATGACAGCGAGCCAATTGGACGCAAAGGGAACCCGACGCGTCCgggc > 2:2726265/1‑148 (MQ=255) cGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTcaa < 2:1929942/150‑1 (MQ=255) gAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTcaaa > 1:5229616/1‑150 (MQ=255) aGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTcaaac > 1:1214229/1‑150 (MQ=255) gCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTcaaaca < 2:1553850/150‑1 (MQ=255) gCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTcaaaca > 1:2619406/1‑150 (MQ=255) cacaAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAACCCGACGCGTCCGGTcaaacaa > 1:4292748/1‑150 (MQ=255) cacaAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCGGAATATTGCGGAGAAAAAGCATGGCCGGGACTGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTcaaacaa > 2:1799059/1‑150 (MQ=255) caAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACaaaa > 1:2692369/1‑150 (MQ=255) aaaTTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAACCAAAAg > 2:5212024/1‑150 (MQ=255) aaaTTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAg > 2:1824367/1‑150 (MQ=255) aaTTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCACAGGGAAACCGACGCGTCCGGTCAAACCACAgg > 2:5017999/1‑150 (MQ=255) aaTTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAgg > 1:283285/1‑150 (MQ=255) aTTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGTCGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGt > 2:3849755/1‑150 (MQ=255) aTTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGt > 2:200789/1‑150 (MQ=255) tttGAGAGATATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTaa > 1:3571386/1‑150 (MQ=255) tGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGc > 1:2145020/1‑150 (MQ=255) tGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGc > 2:393807/1‑150 (MQ=255) agagaTATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCACAGGGAAACCGACGCGTCCGGTCAAACCAAAGGTAAGCcg > 2:3020743/1‑150 (MQ=255) agagaTATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCcg > 1:2597897/1‑150 (MQ=255) agaTATCTTCTTCTGTCTT‑GTAACAGAATAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCcgtc > 1:5239022/1‑150 (MQ=255) gaTATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCCAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCcgtcg > 2:3504751/1‑150 (MQ=255) gaTATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCcgtcg < 2:4692455/150‑1 (MQ=255) atatCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCcgtcgt > 1:3031909/1‑150 (MQ=255) cttctGTCTT‑GTAACAGAATAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACg > 1:3734241/1‑150 (MQ=255) cttctGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACg > 2:2087080/1‑150 (MQ=255) tGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGa < 2:2881601/150‑1 (MQ=255) tGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGa > 2:4176324/1‑150 (MQ=255) tCTT‑GTAACAGAAGAACAGCAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATg < 2:3176543/150‑1 (MQ=255) tt‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGAc < 2:578774/150‑1 (MQ=255) t‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACg > 1:305235/1‑150 (MQ=255) tAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACgat > 1:4929425/1‑150 (MQ=255) aaCAGAATAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACgatg < 1:1287500/150‑1 (MQ=255) aCAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACgatga > 1:5005062/1‑150 (MQ=255) aCAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACgatga < 1:2871749/150‑1 (MQ=255) agaagaACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGAtt > 1:4180357/1‑150 (MQ=255) agaagaACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGAtt < 1:128448/150‑1 (MQ=255) gaagaaCAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTa < 1:377311/150‑1 (MQ=255) aagaaCAGAAAATCGGGCTTTTGCTCATTAATATTGCGGAGAAAAAGCATGGACTGGAATGACATCGAGCCACTTTGACGCAAAGGGAATCCTACGCGTCCGGTCAAACAAAAGGTAAGCAGTCGTCGTTACGAAGATGACGATGATTac < 1:3663372/150‑1 (MQ=255) aagaaCAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTac < 1:5104779/150‑1 (MQ=255) | TTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTT‑GTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGAAACCGACGCGTCCGGTCAAACAAAAGGTAAGCCGTCGTCGTTACGAAGATGACGATGATTACGA > CP009273/161024‑161320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |