Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,403,195 G→T 7.7% S546* (TCG→TAG)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,403,1950GT 7.7% 1139.3 / 51.3 311S546* (TCG→TAG) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base G (159/128);  new base T (18/6);  total (177/134)
Fisher's exact test for biased strand distribution p-value = 8.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 311 total aligned reads displayed.

GATAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCA  >  CP009273/2403047‑2403342
                                                                                                                                                    |                                                                                                                                                   
gATAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGa                                                                                                                                                    >  2:3145169/1‑150 (MQ=255)
 aTAATGGTGAACGGTACCCCCAACTTCACAAATTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAATGCGGCGGATGCTGCCATCGCCACTACCATCGCGAATGGATAAGGCGac                                                                                                                                                   >  1:1837409/1‑150 (MQ=255)
 aTAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTac                                                                                                                                                   >  1:2076709/1‑150 (MQ=255)
 aTAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGac                                                                                                                                                   >  2:1997480/1‑150 (MQ=255)
 aTAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGac                                                                                                                                                   >  1:590849/1‑150 (MQ=255)
  tAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGaca                                                                                                                                                  >  2:435645/1‑150 (MQ=255)
    aTGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACAcc                                                                                                                                                >  1:193714/1‑150 (MQ=255)
          aaCGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTTCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATc                                                                                                                                          <  1:3353858/150‑1 (MQ=255)
           aCGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCg                                                                                                                                         >  2:1526260/1‑150 (MQ=255)
            cGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGt                                                                                                                                        >  1:5215638/1‑150 (MQ=255)
              gTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGttt                                                                                                                                      >  2:2437383/1‑150 (MQ=255)
              gTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGttt                                                                                                                                      >  2:1238687/1‑150 (MQ=255)
               tACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGtttt                                                                                                                                     >  1:2287880/1‑150 (MQ=255)
               tACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGtttt                                                                                                                                     >  2:4570167/1‑150 (MQ=255)
               tACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGtttt                                                                                                                                     <  1:4953018/150‑1 (MQ=255)
               tACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGtttt                                                                                                                                     >  2:1485941/1‑150 (MQ=255)
               tACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGtttt                                                                                                                                     <  2:4795948/150‑1 (MQ=255)
                aCCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGTTTTg                                                                                                                                    >  2:1541414/1‑150 (MQ=255)
                    ccAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGgcag                                                                                                                                <  2:2850571/150‑1 (MQ=255)
                      aaCTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCCTCGCGAATGGATAAGGCGACACCCCCATCGTTTTGgcagca                                                                                                                              >  2:1174636/1‑150 (MQ=255)
                      aaCTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGgcagca                                                                                                                              <  1:2667928/150‑1 (MQ=255)
                        cTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCagcagc                                                                                                                            <  1:539778/150‑1 (MQ=255)
                        cTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCagcagc                                                                                                                            <  1:2902696/150‑1 (MQ=255)
                           cacaAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGcagccag                                                                                                                         >  2:2586391/1‑150 (MQ=255)
                           cacaAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGcagccag                                                                                                                         >  2:550295/1‑150 (MQ=255)
                           cacaAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGcagccag                                                                                                                         >  1:1663376/1‑150 (MQ=255)
                           cacaAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGcagccag                                                                                                                         <  1:398507/150‑1 (MQ=255)
                             caAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCAACGTTTTGGCAGCAGCCAgcg                                                                                                                       >  2:3516679/1‑150 (MQ=255)
                              aaaGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAgcgc                                                                                                                      >  2:4640142/1‑150 (MQ=255)
                                 gTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAAt                                                                                                                   >  1:4858951/1‑150 (MQ=255)
                                  tCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATc                                                                                                                  >  2:3363008/1‑150 (MQ=255)
                                    aCTGAAGCTGTGATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCgg                                                                                                                >  1:746869/1‑150 (MQ=255)
                                      tGAAGCTGTAATTTCCCGGACCTACAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCCTCGTTTTGGCAGCAGCCAGCGCAATCGGAg                                                                                                              <  2:4095104/150‑1 (MQ=255)
                                        aaGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGcc                                                                                                            <  1:5081220/150‑1 (MQ=255)
                                          gCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCAt                                                                                                          <  2:3300497/150‑1 (MQ=255)
                                           cTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATc                                                                                                         >  1:2144716/1‑150 (MQ=255)
                                            tGTAATTTTCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCa                                                                                                        <  1:3919410/150‑1 (MQ=255)
                                             gTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCaa                                                                                                       <  2:1056675/150‑1 (MQ=255)
                                             gTAATGTCCCGGACCTAACACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCaa                                                                                                       <  2:5031710/150‑1 (MQ=255)
                                              tAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAac                                                                                                      >  1:2244335/1‑150 (MQ=255)
                                               aaTTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAaca                                                                                                     <  1:2755839/150‑1 (MQ=255)
                                               aaTTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAaca                                                                                                     >  2:1599620/1‑150 (MQ=255)
                                                aTTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAacac                                                                                                    <  1:274578/150‑1 (MQ=255)
                                                  ttCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACAccg                                                                                                  <  1:2029888/150‑1 (MQ=255)
                                                   tCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACAccgc                                                                                                 >  2:173739/1‑150 (MQ=255)
                                                    cccGGACCCAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACAccgcc                                                                                                <  1:3059388/150‑1 (MQ=255)
                                                      cGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGt                                                                                              <  1:1261466/150‑1 (MQ=255)
                                                       ggACCTAAAACCAGTGTGTTAACAGGTGAAGAATCCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCTTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTc                                                                                             <  1:2520460/150‑1 (MQ=255)
                                                       ggACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTc                                                                                             <  1:5114880/150‑1 (MQ=255)
                                                       ggACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTc                                                                                             >  1:660975/1‑150 (MQ=255)
                                                         aCCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTcgc                                                                                           >  1:1604024/1‑150 (MQ=255)
                                                          ccTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTcgcg                                                                                          <  1:342366/150‑1 (MQ=255)
                                                           cTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCgg                                                                                         >  1:115462/1‑150 (MQ=255)
                                                             aaaaCCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTa                                                                                       <  1:296690/150‑1 (MQ=255)
                                                             aaaaCCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTa                                                                                       <  1:3548125/150‑1 (MQ=255)
                                                               aaCCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTAtt                                                                                     <  1:1575229/150‑1 (MQ=255)
                                                               aaCCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTAtt                                                                                     <  2:1691618/150‑1 (MQ=255)
                                                                   aGTGTGTTAACCGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGCAGGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTagag                                                                                 <  2:1513473/150‑1 (MQ=255)
                                                                   aGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTagag                                                                                 <  1:1893667/150‑1 (MQ=255)
                                                                   aGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAAGGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTagag                                                                                 <  2:5077362/150‑1 (MQ=255)
                                                                    gtgtgtTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAgaga                                                                                >  1:4452373/1‑150 (MQ=255)
                                                                     tgtgtTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGAt                                                                               >  2:4126677/1‑150 (MQ=255)
                                                                     tgtgtTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGAt                                                                               >  1:4185657/1‑150 (MQ=255)
                                                                          tAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATa                                                                          <  2:3462403/150‑1 (MQ=255)
                                                                           aaCAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAg                                                                         <  1:1390683/150‑1 (MQ=255)
                                                                             cAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGACATCAACACCGCCGTCGCGGTATTAGAGATAAATAGcc                                                                       >  1:4224121/1‑150 (MQ=255)
                                                                             cAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGcc                                                                       >  1:2348872/1‑150 (MQ=255)
                                                                               ggTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCa                                                                     <  2:5160627/150‑1 (MQ=255)
                                                                               ggTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCa                                                                     >  1:2422353/1‑150 (MQ=255)
                                                                                 tGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAAt                                                                   >  2:2202381/1‑150 (MQ=255)
                                                                                    agaaACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATAAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAAc                                                                <  1:4095510/150‑1 (MQ=255)
                                                                                      aaaCCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCTACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAAccg                                                              >  1:4666753/1‑150 (MQ=255)
                                                                                      aaaCCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATCAATAGCCCAATAAccg                                                              >  2:4998961/1‑150 (MQ=255)
                                                                                      aaaCCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCACTAAccg                                                              >  2:616227/1‑150 (MQ=255)
                                                                                         ccGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAAGGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAAccgccg                                                           <  2:4875783/150‑1 (MQ=255)
                                                                                           ggTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATCAATAGCCCAATAACCGCCgac                                                         >  2:3635347/1‑150 (MQ=255)
                                                                                           ggTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCgac                                                         >  1:4620136/1‑150 (MQ=255)
                                                                                                   aaaGGCGGCGGATGCTGCCATCGCCACGACCATCGCGACTGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGacaaa                                                 <  2:1834656/150‑1 (MQ=255)
                                                                                                    aaGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGacaaac                                                >  1:841951/1‑150 (MQ=255)
                                                                                                     aGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGacaaaca                                               <  1:1687273/150‑1 (MQ=255)
                                                                                                      ggcggcGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGacaaacaa                                              <  2:3933224/150‑1 (MQ=255)
                                                                                                       gcggcgGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGAcaaacaaa                                             <  2:4965329/150‑1 (MQ=255)
                                                                                                         ggcggATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAaacaaaca                                           >  2:3695112/1‑150 (MQ=255)
                                                                                                          tcggATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAacac                                          <  2:922050/149‑1 (MQ=255)
                                                                                                            ggATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACAccc                                        >  1:4289836/1‑150 (MQ=255)
                                                                                                               tgctgcCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAg                                     <  2:4429825/150‑1 (MQ=255)
                                                                                                                    ccATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGcatca                                >  2:1406883/1‑150 (MQ=255)
                                                                                                                       tCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCatat                             >  1:3187282/1‑150 (MQ=255)
                                                                                                                          ccACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATCtgtg                          >  2:4176945/1‑150 (MQ=255)
                                                                                                                             cGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGcc                       <  2:366830/150‑1 (MQ=255)
                                                                                                                                ccATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGt                    <  2:2261752/150‑1 (MQ=255)
                                                                                                                                   tCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAac                 >  1:1772190/1‑150 (MQ=255)
                                                                                                                                      cgAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAaccac              >  1:944720/1‑150 (MQ=255)
                                                                                                                                         aTGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAATCACCCCAGCATCATATGTGGCCCGTAACCACCGc           >  2:2867087/1‑150 (MQ=255)
                                                                                                                                         aTGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGc           >  1:1021204/1‑150 (MQ=255)
                                                                                                                                            gATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCaa        >  1:1298409/1‑150 (MQ=255)
                                                                                                                                              tAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATg      <  2:4682895/150‑1 (MQ=255)
                                                                                                                                               aaGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGt     >  2:3735640/1‑150 (MQ=255)
                                                                                                                                               aaGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCAACATATATGGCCCGTAACCACCTCCAAtat     >  2:2710911/1‑148 (MQ=255)
                                                                                                                                                  gCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCa  <  1:1706630/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GATAATGGTGAACGGTACCCCCAACTTCACAAAGTCACTGAAGCTGTAATTTCCCGGACCTAAAACCAGTGTGTTAACAGGTGAAGAAACCGGTGTCATAAAGGCGGCGGATGCTGCCATCGCCACGACCATCGCGAATGGATAAGGCGACACCCCCATCGTTTTGGCAGCAGCCAGCGCAATCGGAGCCATCAACACCGCCGTCGCGGTATTAGAGATAAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCA  >  CP009273/2403047‑2403342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑