BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	188,863	T→A	6.1%	D174E (GAT→GAA)	pyrH →	uridylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	188,863	0	T	A	6.1%	1142.6 / 44.9	321	D174E (GAT→GAA)	pyrH	uridylate kinase
Reads supporting (aligned to +/- strand): ref base T (147/154); new base A (13/7); total (160/161)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 325 total aligned reads displayed.

CTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGG  >  CP009273/188714‑189012

cTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAt                                                                                                                                                       >  2:1473377/1‑150 (MQ=255)

cTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAt                                                                                                                                                       <  1:175143/150‑1 (MQ=255)

  gTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATcc                                                                                                                                                     >  1:4876040/1‑150 (MQ=255)

  gTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATcc                                                                                                                                                     >  1:3692584/1‑150 (MQ=255)

  gTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATcc                                                                                                                                                     >  1:2279277/1‑150 (MQ=255)

   ttGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCCCCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAACCg                                                                                                                                                    <  2:4350396/150‑1 (MQ=255)

   ttGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCg                                                                                                                                                    <  1:1149669/150‑1 (MQ=255)

   ttGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCg                                                                                                                                                    <  1:1003069/150‑1 (MQ=255)

      cgcAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCg                                                                                                                                                 <  2:2952778/150‑1 (MQ=255)

       gcAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGa                                                                                                                                                <  1:3814634/150‑1 (MQ=255)

            aacCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGAt                                                                                                                                           >  2:5102716/1‑150 (MQ=255)

             acCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATc                                                                                                                                          <  1:1335539/150‑1 (MQ=255)

               cGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCa                                                                                                                                        <  2:3175858/150‑1 (MQ=255)

               cGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCa                                                                                                                                        <  1:2437526/150‑1 (MQ=255)

               cGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCa                                                                                                                                        >  1:4527084/1‑150 (MQ=255)

                gtgtGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCaa                                                                                                                                       >  2:1936963/1‑150 (MQ=255)

                 tgtgGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAAc                                                                                                                                      <  2:132526/150‑1 (MQ=255)

                 tgtgGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAAc                                                                                                                                      <  2:4004779/150‑1 (MQ=255)

                 tgtgGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAAc                                                                                                                                      <  2:3135155/150‑1 (MQ=255)

                     gtgATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAACGATCCAACCGCa                                                                                                                                  >  2:3596489/1‑150 (MQ=255)

                      tgATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCaa                                                                                                                                 >  2:3812709/1‑150 (MQ=255)

                        aTCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAACCGGCGAAAGATCCAACCGCAAcc                                                                                                                               >  1:2565774/1‑150 (MQ=255)

                          ccTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCAt                                                                                                                             >  2:4069488/1‑150 (MQ=255)

                          ccTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCAt                                                                                                                             <  2:1127400/150‑1 (MQ=255)

                           ctcgCCGCCGGTACAGGTATCCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGACGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATg                                                                                                                            <  2:3087929/150‑1 (MQ=255)

                             ctcCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTa                                                                                                                          <  1:1932834/150‑1 (MQ=255)

                                 gccgGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAg                                                                                                                      <  2:2667885/150‑1 (MQ=255)

                                  ccgGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAAATGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGTCGTGTTTACCGCTGATCAGGCGAAAGATCCAACCGCACACAAGTACGAga                                                                                                                     >  2:3894540/1‑149 (MQ=255)

                                    ggTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCaa                                                                                                                   <  1:4277736/150‑1 (MQ=255)

                                    ggTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCaa                                                                                                                   >  1:65936/1‑150 (MQ=255)

                                     gTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAACCGGCGAAAGATCCAACCGCAACCATGTACGAGCAAc                                                                                                                  >  1:146448/1‑150 (MQ=255)

                                      tACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACt                                                                                                                 <  2:565678/150‑1 (MQ=255)

                                         aGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGAc                                                                                                              >  2:765776/1‑150 (MQ=255)

                                          ggTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACt                                                                                                             >  1:3867508/1‑150 (MQ=255)

                                           gTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACtt                                                                                                            >  2:2100196/1‑150 (MQ=255)

                                           gTAACCCGTTCATTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACtt                                                                                                            <  2:4753863/150‑1 (MQ=255)

                                             aaCCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTTGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTAc                                                                                                          <  2:3026381/150‑1 (MQ=255)

                                                   ttCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGaa                                                                                                    >  2:1619867/1‑150 (MQ=255)

                                                        tACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCt                                                                                               >  2:1279535/1‑150 (MQ=255)

                                                        tACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCt                                                                                               <  2:4647540/150‑1 (MQ=255)

                                                        tACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCt                                                                                               <  2:2042774/150‑1 (MQ=255)

                                                         accaccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTg                                                                                              >  2:1785996/1‑150 (MQ=255)

                                                               gACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGaaaaa                                                                                        >  1:5078929/1‑150 (MQ=255)

                                                                       agcTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTTGTGCTGAAAGCAACCAAAGTTGACGGCTTGTTTACCGCTGATCCGGAGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGaa                                                                                <  2:170256/150‑1 (MQ=255)

                                                                          ttGCCTGCGTTGTATCGAAATTGAAGCCGATGTGGTGCTGATAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGt                                                                             <  2:4925581/150‑1 (MQ=255)

                                                                           tGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTc                                                                            >  2:2444568/1‑150 (MQ=255)

                                                                           tGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTc                                                                            >  1:1432384/1‑150 (MQ=255)

                                                                             ccTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCAt                                                                          <  1:2137985/150‑1 (MQ=255)

                                                                               tGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATgg                                                                        <  2:2872993/150‑1 (MQ=255)

                                                                                 cGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGAc                                                                      <  1:4056381/150‑1 (MQ=255)

                                                                                 cGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGAc                                                                      <  2:1026733/150‑1 (MQ=255)

                                                                                  gTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGAcc                                                                     <  2:4192574/150‑1 (MQ=255)

                                                                                     gTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTgg                                                                  >  1:1643418/1‑150 (MQ=255)

                                                                                       aTCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAACCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTggcg                                                                >  2:1954850/1‑150 (MQ=255)

                                                                                             aTTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTc                                                          >  1:3264903/1‑150 (MQ=255)

                                                                                              ttGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAACCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCa                                                         >  2:2625102/1‑150 (MQ=255)

                                                                                               tGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCAc                                                        >  2:3419644/1‑150 (MQ=255)

                                                                                                gAAGCCGATGTGGTGCTGAAAGCAACCACAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACg                                                       <  2:4665285/150‑1 (MQ=255)

                                                                                                 aaGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACGGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGc                                                      >  1:3294308/1‑150 (MQ=255)

                                                                                                 aaGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGc                                                      >  1:2270377/1‑150 (MQ=255)

                                                                                                  aGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCt                                                     <  1:4687882/150‑1 (MQ=255)

                                                                                                  aGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCt                                                     >  1:4607218/1‑150 (MQ=255)

                                                                                                   gCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTg                                                    <  1:5142483/150‑1 (MQ=255)

                                                                                                    ccGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAACCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTgg                                                   >  1:742245/1‑150 (MQ=255)

                                                                                                      gATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGACAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCt                                                 >  2:3130342/1‑150 (MQ=255)

                                                                                                      gATGTGGTGCTGAAAGCAACCAAAGTTGACGGAGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCt                                                 <  1:1524010/150‑1 (MQ=255)

                                                                                                          tggtgCAGAAAGCAACCAAAGTTGACGGCGTGTTAACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTCCAGCGAACTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCAGGCTCGTg                                             >  2:2363844/1‑150 (MQ=255)

                                                                                                           ggtgCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGa                                            >  1:4455451/1‑150 (MQ=255)

                                                                                                           ggtgCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGa                                            <  1:2948194/150‑1 (MQ=255)

                                                                                                           ggtgCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGa                                            <  2:438551/150‑1 (MQ=255)

                                                                                                            gtgCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGAc                                           >  1:2467820/1‑150 (MQ=255)

                                                                                                            gtgCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGAACCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGAc                                           >  1:2919158/1‑150 (MQ=255)

                                                                                                             tgCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGAcc                                          >  1:287644/1‑150 (MQ=255)

                                                                                                              gCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCa                                         <  1:77766/150‑1 (MQ=255)

                                                                                                                tGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATa                                       <  2:3374701/150‑1 (MQ=255)

                                                                                                                 gAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATaa                                      >  2:2566944/1‑150 (MQ=255)

                                                                                                                 gAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATaa                                      >  1:3391127/1‑150 (MQ=255)

                                                                                                                     gCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTa                                  <  2:4415774/150‑1 (MQ=255)

                                                                                                                      cAACCAAAGTTGCCGGCGTGTTTACCGCTGAACCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTAc                                 <  2:4873793/150‑1 (MQ=255)

                                                                                                                       aaCCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTAcc                                <  2:5049824/150‑1 (MQ=255)

                                                                                                                       aaCCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTAcc                                <  1:4363521/150‑1 (MQ=255)

                                                                                                                         ccAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGa                              >  1:574053/1‑150 (MQ=255)

                                                                                                                          cAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGAt                             <  1:1829501/150‑1 (MQ=255)

                                                                                                                             aGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCGCGTGACCATAAATTACCGATTCg                          >  1:2030440/1‑150 (MQ=255)

                                                                                                                              gTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCgt                         <  2:916931/150‑1 (MQ=255)

                                                                                                                              gTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCgt                         <  2:3540987/150‑1 (MQ=255)

                                                                                                                               ttGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGAGTCgtg                        >  2:4596277/1‑150 (MQ=255)

                                                                                                                                   cGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGtttt                    >  1:3287209/1‑150 (MQ=255)

                                                                                                                                    ggCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTc                   >  1:3100690/1‑150 (MQ=255)

                                                                                                                                    ggCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTc                   >  1:1236706/1‑150 (MQ=255)

                                                                                                                                       gtgtTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAat                >  1:3000458/1‑150 (MQ=255)

                                                                                                                                       gtgtTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAat                <  1:5176358/150‑1 (MQ=255)

                                                                                                                                          tttACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATg             <  1:2606447/150‑1 (MQ=255)

                                                                                                                                           ttACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGa            <  1:1616994/150‑1 (MQ=255)

                                                                                                                                            tACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGaa           <  1:4807449/150‑1 (MQ=255)

                                                                                                                                            tACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGaa           >  2:822669/1‑150 (MQ=255)

                                                                                                                                                gCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACaaa       >  1:4229608/1‑150 (MQ=255)

                                                                                                                                                 cTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAAc      >  1:3153764/1‑150 (MQ=255)

                                                                                                                                                 cTGATCCGGCGAAAGATCCAACCGCAAACATGTACGAGAAACTGACTTACAGCGAAGTTCTGGAAAAAGAGCTGACAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCTTTAATTACAGATTCGTGTTTTCAATATGAACAAAc      <  2:1250571/150‑1 (MQ=255)

                                                                                                                                                  tGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAAcc     <  1:282258/150‑1 (MQ=255)

CTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCAACCATGTACGAGCAACTGACTTACAGCGAAGTGCTGGAAAAAGAGCTGAAAGTCATGGACCTGGCGGCCTTCACGCTGGCTCGTGACCATAAATTACCGATTCGTGTTTTCAATATGAACAAACCGGG  >  CP009273/188714‑189012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`