Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,657,523 C→T 5.4% N241N (AAC→AAT)  suhB → inositol monophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,657,5230CT 5.4% 1191.2 / 33.1 330N241N (AAC→AAT) suhBinositol monophosphatase
Reads supporting (aligned to +/- strand):  ref base C (151/160);  new base T (8/10);  total (160/170)
Fisher's exact test for biased strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

CTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCAC  >  CP009273/2657374‑2657672
                                                                                                                                                     |                                                                                                                                                     
   gCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATc                                                                                                                                                    <  1:1806001/150‑1 (MQ=255)
     tgaCGTCGCGGCGGGTCGTGTTGACGGTTTCTTTGACATCGGTCTGCGCCCGTGGGATTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAATATCGt                                                                                                                                                  <  1:3366785/148‑1 (MQ=255)
      tACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGtt                                                                                                                                                 <  2:450544/150‑1 (MQ=255)
      tACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGtt                                                                                                                                                 <  2:1066286/150‑1 (MQ=255)
        cGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACGTCACCGGTGGTCATAACTACATGCAGACCGGTAACATCGATGc                                                                                                                                               >  2:684927/1‑150 (MQ=255)
         gTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCt                                                                                                                                              <  2:2610282/150‑1 (MQ=255)
         gTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCt                                                                                                                                              >  1:2540707/1‑150 (MQ=255)
           cGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTgg                                                                                                                                            >  1:457415/1‑150 (MQ=255)
              tGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTaa                                                                                                                                         <  1:3796116/150‑1 (MQ=255)
                  ggTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCcg                                                                                                                                     >  2:466490/1‑150 (MQ=255)
                   gTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCcgc                                                                                                                                    >  2:1156347/1‑150 (MQ=255)
                       tgtTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCgt                                                                                                                                <  1:1777252/150‑1 (MQ=255)
                         ttGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCgttg                                                                                                                              <  2:4437613/150‑1 (MQ=255)
                          tGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCgttgt                                                                                                                             >  1:988281/1‑150 (MQ=255)
                           gACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCgttgtt                                                                                                                            >  2:4899658/1‑150 (MQ=255)
                             cGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTaa                                                                                                                          >  1:4345753/1‑150 (MQ=255)
                             cGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTaa                                                                                                                          >  2:1460106/1‑150 (MQ=255)
                                 ttCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGcc                                                                                                                      <  1:5080873/150‑1 (MQ=255)
                                     ttGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGc                                                                                                                  >  2:2177352/1‑150 (MQ=255)
                                      tGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCt                                                                                                                 <  2:5192822/150‑1 (MQ=255)
                                      tGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCt                                                                                                                 <  1:4237861/150‑1 (MQ=255)
                                      tGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCt                                                                                                                 <  1:3194057/150‑1 (MQ=255)
                                        aaaTCGGTCTGCGCCCGTGGGATTTCGCCGCAGGCGAGCTGCTGGTTCGAGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGTTAATAACTACATGCTGACCCGTGATATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCagccagg                                                                                                               >  2:2734612/1‑144 (MQ=255)
                                         aaTCGGTCTGCGCCCGTGGGATTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAATATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGc                                                                                                              >  1:618759/1‑150 (MQ=255)
                                           tCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGa                                                                                                            <  2:23294/150‑1 (MQ=255)
                                           tCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGa                                                                                                            <  2:1994818/150‑1 (MQ=255)
                                            cGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGaa                                                                                                           >  1:1836258/1‑150 (MQ=255)
                                              gTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACa                                                                                                         <  2:338116/150‑1 (MQ=255)
                                               tcagcgcCCGGGGGCCTCCGCCGCAGGCGAGCAGCTGGTTCGCGCAGCGGGCGGCCTCGTCAGCGACTTCACCGGTGGTCATAACTACCTGCTGCCCGGTAACCTCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCAAGCTGGCGAACAt                                                                                                        <  2:4929380/147‑1 (MQ=255)
                                               tCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACAt                                                                                                        >  2:2589853/1‑150 (MQ=255)
                                                cTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATg                                                                                                       <  1:4705169/150‑1 (MQ=255)
                                                 tGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGc                                                                                                      <  1:3455979/150‑1 (MQ=255)
                                                   cgcCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGt                                                                                                    >  1:2143439/1‑150 (MQ=255)
                                                    gcCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTg                                                                                                   >  1:4547937/1‑150 (MQ=255)
                                                     cccGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGa                                                                                                  >  1:5032846/1‑150 (MQ=255)
                                                        gTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGa                                                                                               >  2:1454347/1‑150 (MQ=255)
                                                        gTGGGACTTAGCCGCAGGCGAGCTGAAGGTTCGTGAAGCTGGCGGCATCGTAATCGACTTCACCGGTGGTCATAACAAAATGCAGAACCGTAAAATCAATGATGGTAACCAGCGCGTTGTTAAAGACATTCATGCGAACAAGCGTGActa                                                                                               >  2:1679120/1‑148 (MQ=255)
                                                         tGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAg                                                                                              >  2:4202370/1‑150 (MQ=255)
                                                         tGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAg                                                                                              >  2:1869269/1‑150 (MQ=255)
                                                           ggACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGtt                                                                                            <  1:104452/150‑1 (MQ=255)
                                                              cTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAg                                                                                         >  1:2594066/1‑150 (MQ=255)
                                                               ttCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAATATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAgn                                                                                        <  2:3417700/150‑2 (MQ=255)
                                                               ttCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAATATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGc                                                                                        <  2:2016176/150‑1 (MQ=255)
                                                                tCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCg                                                                                       <  2:1349294/150‑1 (MQ=255)
                                                                  gccgcAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGAc                                                                                     >  2:3978809/1‑150 (MQ=255)
                                                                  gccgcAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGAc                                                                                     >  2:1619774/1‑150 (MQ=255)
                                                                     gcAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGct                                                                                  <  2:3756556/150‑1 (MQ=255)
                                                                     gcAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGct                                                                                  <  2:2241582/150‑1 (MQ=255)
                                                                               tgctgGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGtt                                                                        >  2:861127/1‑150 (MQ=255)
                                                                                gctgGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAATCGTTa                                                                       >  2:2207170/1‑150 (MQ=255)
                                                                                 ctgGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTaa                                                                      <  2:3277120/150‑1 (MQ=255)
                                                                                  tgGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGGTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAAt                                                                     <  2:1188192/150‑1 (MQ=255)
                                                                                    gTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGa                                                                   >  1:4961528/1‑150 (MQ=255)
                                                                                     ttCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGAc                                                                  >  2:1655299/1‑150 (MQ=255)
                                                                                         tGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAg                                                              <  1:2916099/150‑1 (MQ=255)
                                                                                         tGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAg                                                              <  2:1947310/150‑1 (MQ=255)
                                                                                         tGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAg                                                              <  2:3614159/150‑1 (MQ=255)
                                                                                         tGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAg                                                              <  1:4864033/150‑1 (MQ=255)
                                                                                          gAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAgg                                                             <  1:845791/150‑1 (MQ=255)
                                                                                               gggcggCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTCACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGt                                                        >  1:1244352/1‑150 (MQ=255)
                                                                                               gggcggCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGt                                                        >  1:930403/1‑150 (MQ=255)
                                                                                               gggcggCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGt                                                        >  1:663460/1‑150 (MQ=255)
                                                                                               gggcggCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGt                                                        >  1:3732344/1‑150 (MQ=255)
                                                                                                ggcggcATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTg                                                       >  1:1180771/1‑150 (MQ=255)
                                                                                                  cggcATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGat                                                     <  2:1510162/150‑1 (MQ=255)
                                                                                                   ggcATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGata                                                    <  2:4576402/150‑1 (MQ=255)
                                                                                                      aTCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATAtca                                                 <  1:1636161/150‑1 (MQ=255)
                                                                                                       tCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATAtcac                                                <  2:4995926/150‑1 (MQ=255)
                                                                                                       tCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATAtcac                                                <  2:4700231/150‑1 (MQ=255)
                                                                                                         gTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAATATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATAtcactc                                              >  1:2202331/1‑150 (MQ=255)
                                                                                                         gTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATAtcactc                                              >  1:1711991/1‑150 (MQ=255)
                                                                                                          tCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATAtcactca                                             >  1:3156018/1‑150 (MQ=255)
                                                                                                           cAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATAtcactcac                                            >  2:1255382/1‑150 (MQ=255)
                                                                                                           cAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATAtcactcac                                            <  1:3605545/150‑1 (MQ=255)
                                                                                                            aGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCAcc                                           >  2:2125132/1‑150 (MQ=255)
                                                                                                               gACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGc                                        <  1:2009045/150‑1 (MQ=255)
                                                                                                                 cTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGccc                                      <  2:3673212/150‑1 (MQ=255)
                                                                                                                    cACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCg                                   >  2:598575/1‑150 (MQ=255)
                                                                                                                      ccGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGcc                                 <  1:2634593/150‑1 (MQ=255)
                                                                                                                        ggtggtCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCtt                               <  1:1911736/150‑1 (MQ=255)
                                                                                                                           ggtCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCa                            >  1:150625/1‑150 (MQ=255)
                                                                                                                           ggtCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCa                            >  1:299341/1‑150 (MQ=255)
                                                                                                                               aTAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGgcg                        <  2:1215562/150‑1 (MQ=255)
                                                                                                                               aTAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGgcg                        <  1:2325958/150‑1 (MQ=255)
                                                                                                                                 aaCTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCt                      >  2:1762146/1‑150 (MQ=255)
                                                                                                                                  aCTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTa                     >  1:3966521/1‑150 (MQ=255)
                                                                                                                                  aCTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTa                     <  2:1972069/150‑1 (MQ=255)
                                                                                                                                   cTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTAt                    <  2:2724648/150‑1 (MQ=255)
                                                                                                                                     aCATGCTGACCGGTAATATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTc                  <  1:302977/150‑1 (MQ=255)
                                                                                                                                      cATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTcc                 >  1:4415970/1‑150 (MQ=255)
                                                                                                                                             aCCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCATGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATAc          <  2:1884355/150‑1 (MQ=255)
                                                                                                                                              ccGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACt         <  2:4345753/150‑1 (MQ=255)
                                                                                                                                               cGGTAATATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACtt        <  2:3050682/150‑1 (MQ=255)
                                                                                                                                               cGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACtt        >  2:2657042/1‑150 (MQ=255)
                                                                                                                                                 gTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTcc      <  1:4771512/150‑1 (MQ=255)
                                                                                                                                                  tAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGTCTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCt     >  2:410662/1‑150 (MQ=255)
                                                                                                                                                  tAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCt     <  2:3829209/150‑1 (MQ=255)
                                                                                                                                                  tAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCt     <  2:2431879/150‑1 (MQ=255)
                                                                                                                                                    aCATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCa   >  2:885343/1‑150 (MQ=255)
                                                                                                                                                     cATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCAc  <  2:1790743/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAGGCGAGCTGCTGGTTCGTGAAGCGGGCGGCATCGTCAGCGACTTCACCGGTGGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCAC  >  CP009273/2657374‑2657672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑