BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,722,762	G→T	5.5%	noncoding (42/76 nt)	gltW ←	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,722,762	0	G	T	5.5%	757.9 / 21.1	217	noncoding (42/76 nt)	gltW	tRNA‑Glu
Reads supporting (aligned to +/- strand): ref base G (123/82); new base T (7/5); total (130/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 224 total aligned reads displayed.

CAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTC  >  CP009273/2722613‑2722888

 aGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACTGTCTGTTTTGAGATCTTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTAccgc                                                                                                                               >  2:2616471/1‑150 (MQ=255)

  gtgtTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTAccgcc                                                                                                                              >  1:3925461/1‑150 (MQ=255)

  gtgtTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTAccgcc                                                                                                                              >  1:4812405/1‑150 (MQ=255)

    atttaaatttaaaGCTTGATCCAGATTTTTAAAGAGCAAATATAAAAAACAATAATTAACAGTATGTTTTTAGATATTTAGGTCTGAGACTATCACTCACAAAAAACAAAGTGTAGTACCCTAGTGTAATCGAACCCCAGTTACCGCCGt                                                                                                                            >  2:3656763/2‑150 (MQ=12)

     tttCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTg                                                                                                                           <  1:1939075/150‑1 (MQ=255)

       tCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGaa                                                                                                                         >  1:3703391/1‑150 (MQ=255)

       tCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGaa                                                                                                                         <  2:2866623/150‑1 (MQ=255)

        cAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGaaa                                                                                                                        >  2:2514338/1‑150 (MQ=255)

        cAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGaaa                                                                                                                        >  1:3741435/1‑150 (MQ=255)

           ttttCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAggg                                                                                                                     <  1:55008/150‑1 (MQ=255)

            tttCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGc                                                                                                                    >  1:5114424/1‑150 (MQ=255)

            tttCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGc                                                                                                                    <  1:4286062/150‑1 (MQ=255)

            tttCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGc                                                                                                                    >  1:4053539/1‑150 (MQ=255)

             ttCATCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCg                                                                                                                   <  2:3746616/150‑1 (MQ=255)

             ttCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCg                                                                                                                   >  1:1986883/1‑150 (MQ=255)

              tCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCgg                                                                                                                  >  2:899791/1‑150 (MQ=255)

               cAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGgt                                                                                                                 >  2:1878794/1‑150 (MQ=255)

                 gCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGgtgt                                                                                                               <  2:4246581/150‑1 (MQ=255)

                   ttGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTcc                                                                                                             <  1:1036933/150‑1 (MQ=255)

                    tGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTTAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCAAGTGTATACGAAACCATGTTAAAGCAGTGAAATGGCGGTGTCCt                                                                                                            >  2:560737/1‑150 (MQ=18)

                    tGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCt                                                                                                            >  2:560373/1‑150 (MQ=255)

                    tGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCt                                                                                                            <  1:2434285/150‑1 (MQ=255)

                     gATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTGTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTg                                                                                                           >  1:2214394/1‑150 (MQ=255)

                      aTCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTgg                                                                                                          >  1:4788407/1‑150 (MQ=255)

                       tCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTggg                                                                                                         >  2:2236910/1‑150 (MQ=255)

                          aGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCct                                                                                                      <  1:632349/150‑1 (MQ=255)

                          aGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCct                                                                                                      >  2:1630433/1‑150 (MQ=255)

                          aGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCct                                                                                                      >  2:1630474/1‑150 (MQ=255)

                           gATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCctc                                                                                                     >  1:3470678/1‑150 (MQ=255)

                           gATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCctc                                                                                                     >  1:2485659/1‑150 (MQ=255)

                            aTTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCctct                                                                                                    >  2:533152/1‑150 (MQ=255)

                            aTTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCctct                                                                                                    >  2:4281731/1‑150 (MQ=255)

                            aTTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCctct                                                                                                    >  2:532841/1‑150 (MQ=255)

                             tttttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTa                                                                                                   <  2:4112869/150‑1 (MQ=255)

                             tttttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTa                                                                                                   <  2:1080467/150‑1 (MQ=255)

                             tttttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTa                                                                                                   >  1:5208865/1‑150 (MQ=255)

                             tttttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTa                                                                                                   <  2:1520090/150‑1 (MQ=255)

                             tttttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTa                                                                                                   <  2:2481109/150‑1 (MQ=255)

                             tttttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTa                                                                                                   <  2:3440902/150‑1 (MQ=255)

                             tttttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTa                                                                                                   <  2:3441038/150‑1 (MQ=255)

                             tttttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTa                                                                                                   <  1:152881/150‑1 (MQ=255)

                              ttttAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAg                                                                                                  <  1:3589513/150‑1 (MQ=255)

                                 tAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACg                                                                                               <  2:1733749/150‑1 (MQ=255)

                                 tAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACg                                                                                               >  2:3037867/1‑150 (MQ=255)

                                  aaaGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGa                                                                                              >  2:4620103/1‑150 (MQ=255)

                                  aaaGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGa                                                                                              >  2:1110659/1‑150 (MQ=255)

                                    agagCAAATATATCAAACAAGACTTAACAGTCTGTTTTGATATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAg                                                                                            >  2:1201028/1‑150 (MQ=255)

                                      agCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAggg                                                                                          >  1:3776118/1‑150 (MQ=255)

                                       gCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGGGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAgggg                                                                                         >  1:3245809/1‑150 (MQ=255)

                                        cAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCTCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGa                                                                                        <  2:79924/150‑1 (MQ=255)

                                           atatatCAAACTAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGTGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGACTCTAGACGAAGGGGacac                                                                                     <  1:41124/150‑1 (MQ=255)

                                           atatatCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGacac                                                                                     >  1:1000995/1‑150 (MQ=255)

                                           atatatCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGacac                                                                                     >  2:2415585/1‑150 (MQ=255)

                                            tatatCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACg                                                                                    >  1:3289569/1‑150 (MQ=255)

                                            tatatCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACg                                                                                    <  2:3990156/150‑1 (MQ=255)

                                            tatagCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACg                                                                                    <  2:4850673/150‑1 (MQ=255)

                                             atatCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGa                                                                                   >  2:4948481/1‑150 (MQ=255)

                                             atatCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGa                                                                                   >  2:179390/1‑150 (MQ=255)

                                               atCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGaaa                                                                                 >  2:5054346/1‑150 (MQ=255)

                                                tCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCTCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGaaaa                                                                                <  1:3131962/150‑1 (MQ=255)

                                                tCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGaaaa                                                                                >  2:2110691/1‑150 (MQ=255)

                                                 cAAACAAGACTTAACCGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAAt                                                                               <  2:1385502/150‑1 (MQ=255)

                                                  aaaCAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAAtt                                                                              <  1:1216405/150‑1 (MQ=255)

                                                  aaaCAAGACTAAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGAAAGTGGCGTCCCCTAGGGGATACGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCAAGACGCAGGGGACACGaaaagt                                                                              >  2:3101822/1‑148 (MQ=14)

                                                   aaCAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTg                                                                             <  1:1955849/150‑1 (MQ=255)

                                                   aaCAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTg                                                                             <  2:3505939/150‑1 (MQ=255)

                                                    aCAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGc                                                                            >  1:1776050/1‑150 (MQ=255)

                                                    aCAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGc                                                                            >  1:1312293/1‑150 (MQ=255)

                                                      aaGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCtt                                                                          >  1:3512981/1‑150 (MQ=255)

                                                        gACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTAt                                                                        >  1:584550/1‑150 (MQ=255)

                                                        gACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTAt                                                                        >  2:713586/1‑150 (MQ=255)

                                                         aCTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATc                                                                       <  2:4914083/150‑1 (MQ=255)

                                                         aCTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATc                                                                       >  1:4322389/1‑150 (MQ=255)

                                                          cTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCa                                                                      >  1:2488746/1‑150 (MQ=40)

                                                          cTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCa                                                                      >  1:5080819/1‑150 (MQ=40)

                                                           ttAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCAc                                                                     >  1:2760980/1‑150 (MQ=40)

                                                            tAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCAcg                                                                    <  2:455493/150‑1 (MQ=39)

                                                             aaCAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCTCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCAcgc                                                                   >  2:711082/1‑150 (MQ=38)

                                                               cAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGt                                                                 <  1:3997318/150‑1 (MQ=37)

                                                               cAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGt                                                                 <  1:901777/150‑1 (MQ=37)

                                                                aGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGtt                                                                >  1:4625959/1‑150 (MQ=35)

                                                                aGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGtt                                                                >  2:3015762/1‑150 (MQ=35)

                                                                 gTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGCAGGGGACACGAATATTGCTTATCACGCGTTg                                                               <  1:2083015/150‑1 (MQ=25)

                                                                 gTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTg                                                               >  1:4168731/1‑150 (MQ=35)

                                                                  tCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGc                                                              >  1:4728994/1‑150 (MQ=34)

                                                                    tGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGt                                                            <  1:5004496/150‑1 (MQ=32)

                                                                     gTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTg                                                           >  1:2631158/1‑150 (MQ=32)

                                                                       tttGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGat                                                         >  1:2973242/1‑150 (MQ=32)

                                                                         tGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGatat                                                       >  1:2457524/1‑150 (MQ=32)

                                                                         tGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGatat                                                       <  1:451913/150‑1 (MQ=32)

                                                                          gagaTATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATAtt                                                      >  1:4765267/1‑150 (MQ=32)

                                                                           agaTATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATAttt                                                     <  2:4446542/150‑1 (MQ=32)

                                                                            gaTATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATAtttt                                                    <  2:1325570/150‑1 (MQ=32)

                                                                             atatTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTc                                                   <  2:5013425/150‑1 (MQ=32)

                                                                              tatTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCg                                                  >  2:2215689/1‑150 (MQ=32)

                                                                               atTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCgt                                                 >  1:2902583/1‑150 (MQ=32)

                                                                               atTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCgt                                                 >  2:1901066/1‑150 (MQ=32)

                                                                               atTGAGGTCGGAGACTTTCACTCACAAACCAGCAATTGGCGTCCCCTTGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCgt                                                 <  1:3656666/150‑1 (MQ=12)

                                                                                 tGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCgtgt                                               <  1:710335/150‑1 (MQ=31)

                                                                                 tGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCATCTAGACGAAGGGGACACTAAAATTGCTTATCACGCGTTGCGTGATATTTTCgtgt                                               >  2:2199157/1‑150 (MQ=14)

CAGTGTTTCAATTTTCAGCTTGATCCAGATTTTTAAAGAGCAAATATATCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTC  >  CP009273/2722613‑2722888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`