BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,793,020	C→T	6.9%	E112K (GAA→AAA)	ygaC ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,793,020	0	C	T	6.9%	1114.1 / 45.4	319	E112K (GAA→AAA)	ygaC	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (149/148); new base T (9/13); total (158/161)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

AACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCA  >  CP009273/2792871‑2793169

aaCATCGTTGTTTCGTTGTTGATGGGGGCGGTTTATGGCTACTTCCTCGTTTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTc                                                                                                                                                       <  1:254492/150‑1 (MQ=255)

aaCATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTc                                                                                                                                                       >  1:1204575/1‑150 (MQ=255)

   aTCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGcc                                                                                                                                                    >  1:4236695/1‑150 (MQ=255)

   aTCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGcc                                                                                                                                                    <  2:1067370/150‑1 (MQ=255)

    tCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGACGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCa                                                                                                                                                   >  1:197094/1‑150 (MQ=255)

       ttgttTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAAt                                                                                                                                                >  1:3582720/1‑150 (MQ=255)

       ttgttTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAAt                                                                                                                                                <  1:4174039/150‑1 (MQ=255)

          tttCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa                                                                                                                                             >  1:4730126/1‑150 (MQ=255)

            tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAcc                                                                                                                                           <  1:4669237/150‑1 (MQ=255)

            tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAcc                                                                                                                                           <  1:717815/150‑1 (MQ=255)

             cGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCa                                                                                                                                          >  2:2797271/1‑150 (MQ=255)

               aTGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAtt                                                                                                                                        <  1:3357460/150‑1 (MQ=255)

                 gTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCa                                                                                                                                      >  1:1864178/1‑150 (MQ=255)

                 gTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCa                                                                                                                                      >  1:591275/1‑150 (MQ=255)

                       gggggCGGTTTATGGCTACGTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAAc                                                                                                                                <  2:1209283/150‑1 (MQ=255)

                        ggggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACg                                                                                                                               <  1:2932396/150‑1 (MQ=255)

                        ggggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACg                                                                                                                               >  2:828964/1‑150 (MQ=255)

                           gCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTc                                                                                                                            <  1:495608/150‑1 (MQ=255)

                           gCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTc                                                                                                                            <  2:5129676/150‑1 (MQ=255)

                           gCGGTTTATGGCTACTTCCTCGATTATTGCCGCAGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTc                                                                                                                            <  2:9768/150‑1 (MQ=255)

                            cgttttATCTCTACTTCCTCGATTATAGACGCCGACTTTTTATAGTCAGCCGTTAACATCATGTAAATGACTGACTCTTCTTTTCGCGACTGGAATCGCCAGTCGCGTCATTTACTGCCTTCGCCAAATAAACCATTCAAATAACGTTCa                                                                                                                           <  2:4091335/147‑1 (MQ=255)

                            cGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCa                                                                                                                           <  2:3193819/150‑1 (MQ=255)

                             ggTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa                                                                                                                          <  2:235689/150‑1 (MQ=255)

                             ggTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa                                                                                                                          <  2:4710580/150‑1 (MQ=255)

                              gTTAATTGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAACATTCAAATAACGTTCAAg                                                                                                                         >  2:2201995/1‑150 (MQ=255)

                                     gCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAaca                                                                                                                  >  1:1162267/1‑150 (MQ=255)

                                     gCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAGTCAAATAACGTTCAAGCGCAaca                                                                                                                  >  2:1299869/1‑150 (MQ=255)

                                      cTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCCGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTACAAGCGCAacac                                                                                                                 >  2:2665460/1‑150 (MQ=255)

                                      cTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAacac                                                                                                                 >  1:683842/1‑150 (MQ=255)

                                      cTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAacac                                                                                                                 >  2:367107/1‑150 (MQ=255)

                                       tACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcg                                                                                                                >  1:1555604/1‑150 (MQ=255)

                                         cTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTATTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTATCTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcgcg                                                                                                              <  2:706979/150‑1 (MQ=255)

                                            ccTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAAc                                                                                                           <  2:1148556/150‑1 (MQ=255)

                                             cTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACt                                                                                                          <  2:2411806/150‑1 (MQ=255)

                                               cGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaa                                                                                                        >  2:2685415/1‑150 (MQ=255)

                                                gATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaaa                                                                                                       <  1:2039047/150‑1 (MQ=255)

                                                   tagtGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGcc                                                                                                    <  1:465453/147‑1 (MQ=255)

                                                    attGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTTGCCAAATAAACCATACAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCa                                                                                                   >  2:3576563/1‑150 (MQ=255)

                                                    attGCCGCCGACTGTTTAAAGTCAGACGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAGTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCa                                                                                                   >  1:1353034/1‑150 (MQ=255)

                                                     ttGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCAt                                                                                                  <  1:2082245/150‑1 (MQ=255)

                                                      tGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATg                                                                                                 <  1:4422074/150‑1 (MQ=255)

                                                          gccgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCggg                                                                                             <  1:1009713/150‑1 (MQ=255)

                                                            cgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACGGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATACCGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGAt                                                                                           <  1:5172352/150‑1 (MQ=255)

                                                             gACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATa                                                                                          <  2:4760161/150‑1 (MQ=255)

                                                               cTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAcc                                                                                        >  1:4343518/1‑150 (MQ=255)

                                                                  tttAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTa                                                                                     >  1:1244269/1‑150 (MQ=255)

                                                                  tttAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTa                                                                                     <  1:328954/150‑1 (MQ=255)

                                                                    tAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAGGCCATGCGGGATACCGTAAt                                                                                   <  2:2977222/150‑1 (MQ=255)

                                                                     aaaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATg                                                                                  <  1:4636768/150‑1 (MQ=255)

                                                                      aaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGc                                                                                 <  1:4044281/150‑1 (MQ=255)

                                                                        gTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTc                                                                               >  2:2576191/1‑150 (MQ=255)

                                                                        gTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTACGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTc                                                                               <  2:2128969/150‑1 (MQ=255)

                                                                           aGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCgtg                                                                            >  1:3734396/1‑150 (MQ=255)

                                                                               gTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgc                                                                        >  1:3313709/1‑150 (MQ=255)

                                                                                ttACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgct                                                                       >  1:289847/1‑150 (MQ=255)

                                                                                 tACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgctc                                                                      >  1:4961065/1‑150 (MQ=255)

                                                                                 tACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACGGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgctc                                                                      <  1:891008/150‑1 (MQ=255)

                                                                                  aCCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCCAATATACCATTCAAATAACGTTCAAGCGCAACACGCGAAATATAGCCATGCGGGCTCCAGTAATGCTCGTGtggctcg                                                                     >  1:4163570/1‑150 (MQ=255)

                                                                                  aCCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgctcg                                                                     <  1:3025455/150‑1 (MQ=255)

                                                                                    cagcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGATTCGCCAAATAAACCAGTCAAATAACGTTCAAGAGCAACACGCGACCTAAAGCCATGCGGGATACCGTAATGCGCGTGtagctcgac                                                                   >  2:2743980/1‑148 (MQ=255)

                                                                                     agcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCt                                                                  >  1:2417944/1‑150 (MQ=255)

                                                                                      gcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTg                                                                 <  1:3538629/150‑1 (MQ=255)

                                                                                        agGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCt                                                               >  1:2764731/1‑150 (MQ=255)

                                                                                              aaGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATag                                                         >  2:653495/1‑150 (MQ=255)

                                                                                               aGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATaga                                                        <  1:872267/150‑1 (MQ=255)

                                                                                                   tGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTTGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCgg                                                    <  1:3938265/150‑1 (MQ=255)

                                                                                                    gACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGa                                                   <  2:440419/150‑1 (MQ=255)

                                                                                                        cttcttTTCGCGACTGTCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAtt                                               <  2:4936744/150‑1 (MQ=255)

                                                                                                        cttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAtt                                               <  2:1947220/150‑1 (MQ=255)

                                                                                                         ttcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTg                                              <  1:3068836/150‑1 (MQ=255)

                                                                                                          tcttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGc                                             >  2:1415358/1‑150 (MQ=255)

                                                                                                           cttTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCt                                            >  1:1677919/1‑150 (MQ=255)

                                                                                                            ttttCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTg                                           >  1:49076/1‑150 (MQ=255)

                                                                                                                cgcgACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa                                       >  2:1658789/1‑150 (MQ=255)

                                                                                                                  cgACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATg                                     <  2:4981082/150‑1 (MQ=255)

                                                                                                                   gACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGa                                    >  1:2325373/1‑150 (MQ=255)

                                                                                                                    aCTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCTCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGTTACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGAt                                   <  2:4362012/150‑1 (MQ=255)

                                                                                                                     cTGGCATCGCCAGTCGCGTCTTTAACTGGCTTTGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATc                                  <  1:2664964/150‑1 (MQ=255)

                                                                                                                     cTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGACATTGCTGGTAATGATc                                  >  1:4818649/1‑150 (MQ=255)

                                                                                                                       ggCATCGCCAGTCGCGTCTTTAACTGGCTTTGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGc                                <  2:3404505/150‑1 (MQ=255)

                                                                                                                       ggCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGc                                >  1:2889307/1‑150 (MQ=255)

                                                                                                                         cATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCaa                              <  2:1804012/150‑1 (MQ=255)

                                                                                                                          aTCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAg                             <  1:3818483/150‑1 (MQ=255)

                                                                                                                           tCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATACAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGt                            >  1:2641359/1‑150 (MQ=255)

                                                                                                                             gCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGttt                          <  1:214643/150‑1 (MQ=255)

                                                                                                                              ccAGTCGCGTCTTTAACGGGCTTCGCCAAATAAACCATTCAAATCACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGtttt                         <  2:4118114/150‑1 (MQ=255)

                                                                                                                               cAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGAGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCTCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTa                        >  2:4506021/1‑150 (MQ=255)

                                                                                                                                 gTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAat                      >  1:4849024/1‑150 (MQ=255)

                                                                                                                                  tCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAata                     <  2:591275/150‑1 (MQ=255)

                                                                                                                                    gcgTCATTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATc                   <  1:2472946/150‑1 (MQ=255)

                                                                                                                                     cgTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATATAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCg                  >  2:3961131/1‑150 (MQ=255)

                                                                                                                                      gTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCgg                 >  2:1165394/1‑150 (MQ=255)

                                                                                                                                      gTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCgg                 <  2:3313922/150‑1 (MQ=255)

                                                                                                                                         tttAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAg              <  2:4639586/150‑1 (MQ=255)

                                                                                                                                           tAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAGATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCg            >  1:283405/1‑150 (MQ=255)

                                                                                                                                           tAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCg            >  1:5090871/1‑150 (MQ=255)

                                                                                                                                             aCTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGACGCggg          >  2:4574264/1‑150 (MQ=255)

                                                                                                                                              cTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGt         >  1:322337/1‑150 (MQ=255)

                                                                                                                                                  cTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAg     >  2:2140462/1‑150 (MQ=255)

                                                                                                                                                     cGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCa  <  1:4412018/150‑1 (MQ=255)

AACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCA  >  CP009273/2792871‑2793169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`