BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,811,566	C→A	5.4%	noncoding (68/77 nt)	argY ←	tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,811,566	0	C	A	5.4%	621.0 / 14.4	186	noncoding (68/77 nt)	argY	tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base C (77/97); new base A (5/7); total (82/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

ATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGAT  >  CP009273/2811417‑2811715

atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                                                                                       <  1:2077705/150‑1 (MQ=40)

  ggTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCgg                                                                                                                                                     <  2:1461138/150‑1 (MQ=40)

      cATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCAGggagg                                                                                                                                                 <  2:2087916/150‑1 (MQ=255)

       aTCCGGGAGGATTCCAAACTACGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGggagga                                                                                                                                                <  1:1458174/150‑1 (MQ=255)

         ccGGGAGGCTTCTAACCTCAGACCGCTAGGTTCGTAGACGAGTACTCGATCCAGGTGTGCTTCGGATGCATCGGT‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCAGGGAGGAtt                                                                                                                                              <  2:4029766/150‑1 (MQ=14)

                 aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCCGGAGGATACGAACCTc                                                                                                                                      >  2:4690864/1‑150 (MQ=255)

                  ttCGAACCTCCGACCGCTCTGTTCGTAGCCGTGTACTCTATCCAGCTGAGCTACGGATGCATCGGT‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTcc                                                                                                                                     <  1:4732666/150‑1 (MQ=255)

                       aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCAGGGAGGATTCGAACCTCCGACCg                                                                                                                                <  2:4620606/150‑1 (MQ=255)

                         cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGcg                                                                                                                              >  2:3067038/1‑149 (MQ=255)

                         cTCCGACCGCTCGGTTCGAAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCGGATATTGATACCGATAATAAAGCACTATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGTAACTCAGAgcgca                                                                                                                              >  2:4752241/1‑149 (MQ=14)

                          tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGTG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                                             <  2:1832345/150‑1 (MQ=255)

                          tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGcgc                                                                                                                             >  2:3389509/1‑148 (MQ=255)

                              aCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCACGTAAGAGAGGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                                                                                         >  2:4952837/1‑150 (MQ=255)

                               ccGCTCGGTTCGTAGCCGTGTTCTCTATCCTGCTGCGCTTCGGATGCATCGGG‑ATATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATGTAGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                                        <  1:59706/150‑1 (MQ=18)

                               ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGTGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                                        <  1:1635456/150‑1 (MQ=255)

                               ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATTCCGCTACTAAAGCCATATCAAGTAAGTGTTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                                        <  1:1160397/150‑1 (MQ=255)

                                cGCACGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCg                                                                                                                       <  2:2509856/150‑1 (MQ=255)

                                  cTCGGTTCGTAGCCGTGTACTCTATCCAGCTGAGCTACTTATGCATCGGT‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                                                     <  2:1042652/150‑1 (MQ=255)

                                  cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                                                     <  2:2369995/150‑1 (MQ=255)

                                   tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                                                                                    >  1:430779/1‑150 (MQ=255)

                                     ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                                                  <  1:401884/150‑1 (MQ=255)

                                      gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTCGCCg                                                                                                                 >  2:1364500/1‑150 (MQ=255)

                                      gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTCGCCg                                                                                                                 >  2:1868275/1‑150 (MQ=255)

                                          gTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa                                                                                                             >  1:737412/1‑150 (MQ=255)

                                             gCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTActc                                                                                                          <  1:4469358/150‑1 (MQ=255)

                                              ccGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTAACTGCAGATAATTTTAACGCTAATAAATCCATATAAATTAAGAGATGGTTCATAAGGGAGGAATAGAACCACGTACCGCTCGGATCGTTGCCCAGTCctat                                                                                                         >  1:3133636/1‑148 (MQ=9)

                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                                                        >  1:2445867/1‑150 (MQ=255)

                                               cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACACTa                                                                                                        >  2:4964475/1‑150 (MQ=255)

                                                 aGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGGTCGTAGCCGAGTACTCTATc                                                                                                      >  2:3248229/1‑150 (MQ=255)

                                                  gTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAtac                                                                                                     >  2:2717361/1‑148 (MQ=255)

                                                   tACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa                                                                                                    >  2:3829604/1‑150 (MQ=255)

                                                    aCTCGATCCAGATGTGCTACGTATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                                                   <  2:359180/150‑1 (MQ=255)

                                                      tctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                                                 >  1:3052125/1‑150 (MQ=255)

                                                       ctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                                                >  1:4077979/1‑150 (MQ=255)

                                                         aTCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTAATAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                                              <  1:1689446/150‑1 (MQ=255)

                                                          tCCAGCTGAGCTCCGGATGCATCGGG‑AAAATTATATAACTCCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGCAGGATACGAACCTCCGACCCCTCCGGTCGTAGCCGAGTCCTCTAACCAGcaaagc                                                                                             >  2:1865385/1‑145 (MQ=18)

                                                           ccAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                                                            >  1:1483243/1‑150 (MQ=255)

                                                           ccAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                                                            >  1:1483175/1‑150 (MQ=255)

                                                             atcTGATCTTCCGATTCATCGGG‑AAATTTATTTTACTGCAGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                                                                                          <  1:4719149/148‑1 (MQ=25)

                                                              gcagttcTACCGCTGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTAATACCGCCATATAAAGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                                                                         <  2:3742205/144‑1 (MQ=21)

                                                              gCTGTGCAACGGCTGCATCGGG‑AAAGTTATTTTACTGCTGATATTGAGTCCGCTACTTTCGCCATATGAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                                                                         <  1:3849118/150‑1 (MQ=21)

                                                                    cTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCa                                                                                   >  1:154237/1‑150 (MQ=255)

                                                                     tACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATAAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAt                                                                                  <  2:629843/150‑1 (MQ=255)

                                                                           tGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaa                                                                            >  2:664541/1‑150 (MQ=255)

                                                                             cATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                                                     >  1:4962716/1‑108 (MQ=14)

                                                                             cATCGGG‑AAATTTATTTTACTGCTGATCTTGATTCCGCTACTATCGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAac                                                                          <  1:1731254/150‑1 (MQ=255)

                                                                             cATCGGG‑AAATTTATATGACGGCTGATATTGATACCGCTACTAACGCCATATCTAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAac                                                                          <  2:414353/150‑1 (MQ=21)

                                                                              aTCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTATAGCCATATCAAGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAact                                                                         <  2:197480/150‑1 (MQ=255)

                                                                              aTCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAACGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAact                                                                         <  1:3425342/150‑1 (MQ=255)

                                                                                cGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAactta                                                                       >  1:1025008/1‑150 (MQ=255)

                                                                                 ggg‑ACATGTATTTTACTGCTGATATTGATACCGCTACTAACGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttac                                                                      <  2:1555636/150‑1 (MQ=255)

                                                                                  gg‑ATATTTTTATTACGGTTGATATTGATACCGTTACTATAGCAATATCTAGTATGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttact                                                                     <  1:757595/150‑1 (MQ=17)

                                                                                       atttatttTACTGCTGATATTGCTTCCGCTACTATCGCCATATCTAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttac                                                                 <  2:2917523/150‑1 (MQ=18)

                                                                                        tttatttTACTGCTGATATTGATAACGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACttactttact                                                                <  1:1995606/150‑1 (MQ=255)

                                                                                           atttTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCAGAGCTACGGATGCATCGGGAAACTTACTTTACTGCa                                                             >  1:4184418/1‑150 (MQ=255)

                                                                                           attcTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTc                                                                                                                                             <  1:3539383/70‑1 (MQ=14)

                                                                                           attcTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTc                                                                                                                                             >  2:3539383/1‑70 (MQ=14)

                                                                                                aCTGCTGATATTGTTACCGCTACTACAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAttt                                                        <  1:3214658/150‑1 (MQ=255)

                                                                                                aCTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAttt                                                        >  2:3147107/1‑150 (MQ=255)

                                                                                                aCTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAttt                                                        >  2:217059/1‑150 (MQ=255)

                                                                                                aCTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAttt                                                        <  1:3215089/150‑1 (MQ=255)

                                                                                                 ctgctgATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCAGAGTACTATATACAGGTGGGCTACGGGTGCATCGGGTGACTTAGTTGACTGCGGAtgtg                                                       >  2:4502411/1‑147 (MQ=21)

                                                                                                   gctgATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAACAAGAAGATCTACGTATGCATCGGGAAACTTACTTTACTGCAGAtttttt                                                     >  1:5141791/1‑150 (MQ=21)

                                                                                                   gatgATATTGATACCGCTACTAAAGCCATATCAAGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAtttttt                                                     <  2:4162871/148‑1 (MQ=255)

                                                                                                     tgATATTTCTACCGCTTCTTAATCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGa                                                   <  1:277323/150‑1 (MQ=18)

                                                                                                       atatTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCCGCTGAGCTACGGATGCATCGGGAAACTTACGTTACTGCAGATTTTTTGATa                                                 >  2:4546766/1‑150 (MQ=255)

                                                                                                        ttttGATACCGCTACTAAATCAATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATAc                                                <  2:5216227/148‑1 (MQ=255)

                                                                                                         atTGATACCGCTACTAAAGCAATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATAcc                                               <  2:501722/150‑1 (MQ=255)

                                                                                                              tACCGCTACTAAAGCCATATCAAGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGctac                                          <  2:4357084/150‑1 (MQ=255)

                                                                                                                ccGCTACTATAGCCATATCAAGTAAGAGATGGTGCATCATGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGctacta                                        <  2:3133555/150‑1 (MQ=255)

                                                                                                                   ctactaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                     <  1:2890633/150‑1 (MQ=255)

                                                                                                                    tactaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCCTCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGc                                    >  2:2759248/1‑150 (MQ=255)

                                                                                                                    tactaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGc                                    >  2:3471280/1‑150 (MQ=255)

                                                                                                                    tactaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGc                                    <  1:3837068/150‑1 (MQ=255)

                                                                                                                     actaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGcc                                   >  1:5128080/1‑150 (MQ=255)

                                                                                                                      ctaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTGCTGCAGATTTTTTGATACCGCTACTAAAGCCg                                  >  2:785797/1‑150 (MQ=255)

                                                                                                                        acaGCCATATCAAGTAAGAGATGGTGCATCAGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTa                                <  1:900340/148‑1 (MQ=255)

                                                                                                                           ggcATATCTCGTAAGAGATGGTGCATCCGGGATTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGATTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                             <  2:3028753/148‑1 (MQ=17)

                                                                                                                           gCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTATCCGAGGACACAAACCAGCTGATCAACGCAAGCGTCTGGAAAAGTAATTTCCTGCAGATGTTTTGATACCGCTACTAAAGCCGTATCa                             >  1:1198820/1‑150 (MQ=17)

                                                                                                                           gCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                             >  1:3332018/1‑150 (MQ=255)

                                                                                                                             cATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAg                           >  2:4172870/1‑150 (MQ=255)

                                                                                                                              atatCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAACGGGAAACTTACTGTACTGCAGATGTGATGATCCCGCTACTAAAGCCGTATAAAGt                          >  1:412499/1‑150 (MQ=17)

                                                                                                                                atctcttAAGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTaa                        <  2:4039510/144‑1 (MQ=255)

                                                                                                                                atCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTaa                        <  1:3948813/150‑1 (MQ=255)

                                                                                                                                    aGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa                                                                                                    <  2:3427362/70‑1 (MQ=255)

                                                                                                                                    aGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCa                                                                                                    >  1:3427362/1‑70 (MQ=255)

                                                                                                                                    aGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAACCAGCTGAGCTCCGGATGCATCGGGAAACTTACTGTACTGCATATATTTTGATACCGCTACTAAATCCGTATCAAGTAAgaga                    >  2:4625378/1‑150 (MQ=17)

                                                                                                                                      tatgagaTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATg                  <  2:1777698/147‑1 (MQ=255)

                                                                                                                                         gagaTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTg               <  1:1037004/150‑1 (MQ=255)

                                                                                                                                         aataTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGc                                                              <  1:3162212/100‑1 (MQ=17)

                                                                                                                                          ataTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATAcc                                               <  2:320752/115‑1 (MQ=255)

                                                                                                                                          ataTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATAcc                                               >  1:320752/3‑117 (MQ=255)

                                                                                                                                            aTGGTGCCTCCGGGTGTATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCAt            <  2:4664338/150‑1 (MQ=255)

                                                                                                                                            aTGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCAt            <  2:4664386/150‑1 (MQ=255)

                                                                                                                                             tGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATc           <  1:3539586/150‑1 (MQ=255)

                                                                                                                                              ggTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTACTTTACTGCAGATATTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATcc          <  2:2889510/150‑1 (MQ=255)

                                                                                                                                              ggTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAATCTATCCAGCTGAGCTACGGATGCATCGGTAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATcc          <  1:2288385/150‑1 (MQ=255)

                                                                                                                                                     ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGggagga   <  2:4796642/150‑1 (MQ=255)

                                                                                                                                                      cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGAt  >  1:1398418/1‑150 (MQ=255)

                                                                                                                                                      cGGGAGGATTCGAACCTCCGACCGCTAGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGAt  >  1:4417678/1‑150 (MQ=255)

ATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGAT  >  CP009273/2811417‑2811715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`