Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,893,420 C→A 7.0% M71I (ATG→ATT)  ygcW ← putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,893,4200CA 7.0% 1042.0 / 42.6 284M71I (ATG→ATT) ygcWputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (142/122);  new base A (9/11);  total (151/133)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 289 total aligned reads displayed.

ATCGGATCCCAGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAAT  >  CP009273/2893271‑2893569
                                                                                                                                                     |                                                                                                                                                     
atcGGATCCCTGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACCAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGc                                                                                                                                                       <  2:3996388/150‑1 (MQ=255)
   ggATCCCAGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAACCGCTCACAGCACGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATg                                                                                                                                                    >  2:4515520/1‑150 (MQ=255)
   ggATCCCAGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATg                                                                                                                                                    <  2:944760/150‑1 (MQ=255)
    gATCCCAGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGa                                                                                                                                                   <  1:2212815/150‑1 (MQ=255)
    gATCCCAGTCGGCACGACCGAAGTCCAGCACCTTATTCAACTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGa                                                                                                                                                   <  1:106328/150‑1 (MQ=255)
     aTCCCAGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGaa                                                                                                                                                  <  1:4705473/150‑1 (MQ=255)
      tCCCAGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAg                                                                                                                                                 <  1:1277675/150‑1 (MQ=255)
         cAGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTcc                                                                                                                                              >  2:1796230/1‑150 (MQ=255)
          aGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCa                                                                                                                                             >  2:752884/1‑150 (MQ=255)
            tCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCAcc                                                                                                                                           >  1:1033625/1‑150 (MQ=255)
            tCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCAcc                                                                                                                                           >  1:1988617/1‑150 (MQ=255)
             cGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCt                                                                                                                                          <  1:848972/150‑1 (MQ=255)
              ggCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGAATGAAGTCCACCTc                                                                                                                                         <  2:192132/150‑1 (MQ=255)
                 aCGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAac                                                                                                                                      >  1:3101727/1‑150 (MQ=255)
                   gACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAacac                                                                                                                                    >  1:1113273/1‑150 (MQ=255)
                         aGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGtt                                                                                                                              <  2:655795/150‑1 (MQ=255)
                          gTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGttt                                                                                                                             <  1:4622306/150‑1 (MQ=255)
                           tCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATACTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGtttt                                                                                                                            <  2:1288544/150‑1 (MQ=255)
                           tCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGtttt                                                                                                                            <  2:248910/150‑1 (MQ=255)
                           tCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGtgtt                                                                                                                            >  2:3591873/1‑150 (MQ=255)
                           tCCAGCACCTAATTCAGCTGACAAAGACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGtttt                                                                                                                            <  2:2813981/150‑1 (MQ=255)
                                 aCCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATc                                                                                                                      >  1:3585243/1‑150 (MQ=255)
                                   cTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCAt                                                                                                                    <  1:2239405/150‑1 (MQ=255)
                                   cTTATTCAGCTTACAAAGACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCAt                                                                                                                    <  2:3114861/150‑1 (MQ=255)
                                     tATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCAttt                                                                                                                  <  1:5048094/150‑1 (MQ=255)
                                     tATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTATGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTTTTTTTCAATCAttt                                                                                                                  <  1:2564135/150‑1 (MQ=255)
                                      aTTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTc                                                                                                                 >  2:906280/1‑150 (MQ=255)
                                         cAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCtt                                                                                                              >  2:1412283/1‑150 (MQ=255)
                                               aCAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTc                                                                                                        <  1:333879/150‑1 (MQ=255)
                                                       cGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTAt                                                                                                <  2:3368377/150‑1 (MQ=255)
                                                       cGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTAt                                                                                                <  2:5051925/150‑1 (MQ=255)
                                                        ggCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATc                                                                                               >  2:2291782/1‑150 (MQ=255)
                                                        ggCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATc                                                                                               >  2:5084328/1‑150 (MQ=255)
                                                         gCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCt                                                                                              >  2:394385/1‑150 (MQ=255)
                                                           aTTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCttt                                                                                            >  2:1614646/1‑150 (MQ=255)
                                                           aTTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCttt                                                                                            >  2:4126623/1‑150 (MQ=255)
                                                           aTTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCttt                                                                                            >  1:694375/1‑150 (MQ=255)
                                                           aTTGTTAACCAGAATATCAACGGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCttt                                                                                            <  1:1881453/150‑1 (MQ=255)
                                                             tGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGa                                                                                          >  1:3421954/1‑150 (MQ=255)
                                                             tGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGa                                                                                          >  2:518268/1‑150 (MQ=255)
                                                              gTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCACGACGCCCCCCCCCCCCCCCAGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGAc                                                                                         <  2:409451/150‑1 (MQ=255)
                                                                tAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGa                                                                                       >  1:4941799/1‑150 (MQ=255)
                                                                 aaCCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGaa                                                                                      <  1:250315/150‑1 (MQ=255)
                                                                   ccAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAAc                                                                                    <  2:2419326/150‑1 (MQ=255)
                                                                    cAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACt                                                                                   >  2:5142662/1‑150 (MQ=255)
                                                                      gAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAg                                                                                 >  1:992652/1‑150 (MQ=255)
                                                                        atatCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGa                                                                               >  2:1765528/1‑150 (MQ=255)
                                                                        atatCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGa                                                                               >  2:88551/1‑150 (MQ=255)
                                                                         tatCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGaa                                                                              >  2:2055579/1‑150 (MQ=255)
                                                                         tatCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGaa                                                                              >  1:2549592/1‑150 (MQ=255)
                                                                            cAACTGGACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATaa                                                                           <  2:1726213/150‑1 (MQ=255)
                                                                              aCTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAg                                                                         >  1:1617776/1‑150 (MQ=255)
                                                                                tGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGAATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGat                                                                       <  1:2280808/150‑1 (MQ=255)
                                                                                  tACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGatat                                                                     <  1:4326591/150‑1 (MQ=255)
                                                                                     cGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTg                                                                  >  1:1576019/1‑150 (MQ=255)
                                                                                     cGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTg                                                                  <  1:4893800/150‑1 (MQ=255)
                                                                                       aaaCGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGAATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTgcg                                                                >  1:235424/1‑150 (MQ=255)
                                                                                        aaCGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTgcgc                                                               >  1:4259907/1‑150 (MQ=255)
                                                                                        aaCGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTgcgc                                                               >  1:1735863/1‑150 (MQ=255)
                                                                                          cGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGAATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTAGTTTCGCCGTTATCTTTGACGAAACTAGGACTAAAGATATTTGCGCCa                                                             >  2:3242807/1‑150 (MQ=255)
                                                                                             tCACAGCAAGCAGCGATCATCGTCTGCGGCGCGCCTTCTGCGGTGATTCCCACCTGCATGAAGTCCACCTAAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAATGATATTTGCGCCAGCt                                                          <  2:5241297/150‑1 (MQ=255)
                                                                                                 agcaagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTgg                                                      <  1:2175509/150‑1 (MQ=255)
                                                                                                   caagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGcc                                                    >  1:3989183/1‑150 (MQ=255)
                                                                                                   caagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGcc                                                    >  2:4245925/1‑150 (MQ=255)
                                                                                                    aagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCa                                                   >  2:1773398/1‑150 (MQ=255)
                                                                                                    aagcaGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTACACCTCAACACCCTGTTTTTCAATCATTTCATTTGTTTCGCCGTTATATTTTACGAAACTATGAATAAAGATATATGCGCCAGCTTTGGACa                                                   >  2:4852769/1‑150 (MQ=255)
                                                                                                     agcagcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCaa                                                  >  2:1956741/1‑150 (MQ=255)
                                                                                                        agcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGc                                               >  2:2093967/1‑150 (MQ=255)
                                                                                                        agcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGc                                               >  1:906864/1‑150 (MQ=255)
                                                                                                         gcGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGcc                                              <  1:713105/150‑1 (MQ=255)
                                                                                                          cGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCa                                             >  1:3706860/1‑150 (MQ=255)
                                                                                                           gATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCAt                                            >  1:6074/1‑150 (MQ=255)
                                                                                                           gATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACACCAt                                            >  2:1187720/1‑150 (MQ=255)
                                                                                                           gATAATCTTCTGCGGCGCGCCTACTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCAt                                            >  2:1544481/1‑150 (MQ=255)
                                                                                                            aTAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGAATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATg                                           >  2:3791696/1‑150 (MQ=255)
                                                                                                              aaTCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGc                                         >  2:4139094/1‑150 (MQ=255)
                                                                                                                 cttctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCaaa                                      >  2:2607612/1‑150 (MQ=255)
                                                                                                                  ttctGCGGCTCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAAt                                     <  1:2674230/150‑1 (MQ=255)
                                                                                                                  ttctGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAAt                                     >  2:1325104/1‑150 (MQ=255)
                                                                                                                       cggcgCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTg                                <  2:273727/150‑1 (MQ=255)
                                                                                                                          cgcgcCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGcc                             <  1:1677398/150‑1 (MQ=255)
                                                                                                                           gcgcCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCt                            <  1:2327651/150‑1 (MQ=255)
                                                                                                                            cgcCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTa                           >  1:2902278/1‑150 (MQ=255)
                                                                                                                              ccTTCTGCGGTGATACCCACCTGCATGAAGTCCACCCCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTaaa                         <  2:4721377/150‑1 (MQ=255)
                                                                                                                               cTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAAc                        <  2:612016/150‑1 (MQ=255)
                                                                                                                               cTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTGTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAACCTAGGAATAAAGAGATTTGCGCCAGCTGTGGCCAACGCCATGGCTAATGCCTGGCCTCAAc                        >  2:4986321/1‑150 (MQ=255)
                                                                                                                                 tCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCg                      >  2:2255522/1‑150 (MQ=255)
                                                                                                                                   tGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCt                    <  2:4317089/150‑1 (MQ=255)
                                                                                                                                   tGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCt                    >  1:3459667/1‑150 (MQ=255)
                                                                                                                                   tGCGGTGATACCCACCTGAATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCt                    <  1:2477498/150‑1 (MQ=255)
                                                                                                                                   tGCGGTGATACCCACCTGAATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCt                    <  2:2009832/150‑1 (MQ=255)
                                                                                                                                    gCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTa                   <  2:3342325/150‑1 (MQ=255)
                                                                                                                                       gTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTc                >  2:4789372/1‑150 (MQ=255)
                                                                                                                                         gATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTccc              <  2:4462228/150‑1 (MQ=255)
                                                                                                                                             cccACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCg          <  1:828018/150‑1 (MQ=255)
                                                                                                                                              ccACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCgg         >  1:319665/1‑150 (MQ=255)
                                                                                                                                               cACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGAGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGt        >  1:2014139/1‑150 (MQ=255)
                                                                                                                                                aCCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTa       >  1:1377418/1‑150 (MQ=255)
                                                                                                                                                    gCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACaa   <  1:5084328/150‑1 (MQ=255)
                                                                                                                                                     cATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAAt  <  1:4066168/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCGGATCCCAGTCGGCACGACCGAAGTCCAGCACCTTATTCAGCTTACAAATACCGGCATTGTTAACCAGAATATCAACTGTACCGAAACGCTCACAGCAAGCAGCGATAATCTTCTGCGGCGCGCCTTCTGCGGTGATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCCAGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAAT  >  CP009273/2893271‑2893569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑